Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,813,072	0	A	C	58.8%	5.0 / 14.7	17	T114T (ACA→ACC)	dfp	fused 4'‑phosphopantothenoylcysteine decarboxylase/phosphopantothenoylcysteine synthetase, FMN‑binding
Reads supporting (aligned to +/- strand):  ref base A (5/2);  new base C (0/10);  total (5/12)
Fisher's exact test for biased strand distribution p-value = 3.39e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.85e-02

TGGACCCGGCAGCCGAAGCCGCTATGGGCCATATTGAGCTGGGTAAATGGGCTGATTTAGTGATTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGG  >  NC_000913/3812924‑3813206                                                                                                                                                     |                                                                                                                                       tgGACCCGGCAGCCGAAGCCGCTATGGGCCATATTGAGCTGGGTAAATGGGCTGATTTAGTGATTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACAcc                                                                                                                                      <  2:29348/151‑1 (MQ=255)                               atatTGAGCTGGGTAAATGGGCTGATTTAGTGATTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGaa                                                                                                        >  1:121278/1‑151 (MQ=255)                               atatTGAGCTGGGTAAATGGGCTGATTTAGTGATTCTCGCCCCTGCCACGGCAGATTTGATTGCCAGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGaa                                                                                                        >  2:351395/1‑151 (MQ=255)                                         gggTAAATGGGCTGATTTAGTGATTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGACTGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAATACGCAGATg                                                                                              >  1:126157/1‑151 (MQ=255)                                           gTAAATGGGCTGATTTAGTGATTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTa                                                                                            >  2:75681/1‑151 (MQ=255)                                            tAAGTGGGCTGATTTGGTGATTCTCGCCCCTGCCACGGCAGATTGGATGCCCCGTGTTGCTGCGGGAATGGCAAATGCCCTGTTATCGCCGATTTGTCTGGCTACACCGGCGCCTGTGGCCGTGCCCCCCGCCATGAACCAGCAGATGTAc                                                                                           <  1:7865/151‑1 (MQ=255)                                              aaTGGGCTGATTTAGTGATTCTCGCCCCTGCCCCGGCAGATTTGATTGCCCGTGTGGCTGCCGGAATGGGGAATGGCCTGGTATCGCCGATTTGTCTGCCTACCCGTGCGCCTTTAGCCGTGCCCCCCGCCATGAACCAGCAGATGTTCCg                                                                                         <  2:160247/151‑1 (MQ=255)                                                    ctgttttggggttcctcCCCCCGGCCCCGCCAGTTTTGTTTGCCCGTGTTGCTGCCGGAATGGCGATTGCCCTGGTACCCACAATTTTTCTGGCTCCCCCTCCCCCTGTGGCCGTCCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGc                                                                                   <  2:121301/137‑1 (MQ=255)                                                                    ccccccTGCCACGGCAGTTTTGTTTCCCGGTGTTGCTGCCGAAATGGCGAATGCCCTGTTATCGACGATTTGGCGGGCTACCCCTGCGCCTGTGGCCGTGCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAAt                                                                   <  2:53763/149‑1 (MQ=255)                                                                      ccccTGCCCCGGCAGATTTGATTCCCCTTTTTGCTGCCGGAATGGCGAATGCCCTGGTATCGACGATTTTTCTGGCTACCCCTGCGCCTGTAGCCGTGCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAAttt                                                                 <  2:266423/151‑1 (MQ=255)                                                                            ccACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGGGAATGCCCGGGTATCGACGATTTGTCTGGCAACCCCCGCGCCTGTAGCCGGGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAgg                                                            <  2:313899/150‑1 (MQ=255)                                                                            caaCGGCAATTTTGATTGCCCTTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTTTCTGGCTACCCCTGCCCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAgg                                                            <  1:386712/148‑1 (MQ=255)                                                                                             ccccGTGTTGCTGCCGGAAGGGCGAATGCCTTGGTAGCGACTATTTGTCTGGCTACCCCTCCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGtt                                          <  1:351306/150‑1 (MQ=255)                                                                                              cccGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACCCCTGCCCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTgcttgctt                                                   <  2:251891/141‑1 (MQ=255)                                                                                              cccGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTgcttgctt                                                   >  1:251818/1‑141 (MQ=255)                                                                                                   tttGCTGCCGGAAGGGCAAATGACCGGGTACCGACGATTTTTCTGCCTACCCCTGCCCCGGTAGCCGGGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCa                                    <  1:304166/150‑1 (MQ=255)                                                                                                                                     cgttttgttggccTACCCCTGCGCTTGTCGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTgtgg  <  1:341468/139‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                       TGGACCCGGCAGCCGAAGCCGCTATGGGCCATATTGAGCTGGGTAAATGGGCTGATTTAGTGATTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGG  >  NC_000913/3812924‑3813206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑