New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2840164	89 (1.220)	5 (0.070)	5/278	6.9	5.4%	coding (641/1458 nt)	ascF	cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components
	?	NC_000913	= 2840252	91 (1.330)					coding (729/1458 nt)	ascF	cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below cutoff threshold.

CGCTGATGGTCGCGGCATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2840008‑2840164 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggtcaccgcCGGTGTAAT‑‑GCTGTCCACCCAGCGTTCGATATATGACAGGCACCAGGTCATCACCAGCGCCGGGATCACCGTGTAGGTGTATTTCACCGCGGTGACCGGAATCAGGGCAAATTCGACATGTTCACCCTGGGCCGCTTTCGCCATCAGTTC  <  NC_000913/2840252‑2840103                                                                                                                                                                                                                                                                                                                       CGCTGATGGTCGCGGCATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTTGAGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAGCATGTCGAATTTGCCCTGATTCCGGT                                                                                                                                                                <  2:65584/151‑1   CTGATGGTCGCGGCATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCA                                                                                                                                                              >  1:594669/1‑151   CTGATGGTCGCGGCATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTC                                                                                                                                                               <  1:629984/150‑1                                        CCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCCGGTGTAAT‑‑GCTGTCCACCCAGCGTTCGATATA                                                                                                                       >  1:1166273/1‑151                                             ATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCG‑CGGTGAAATACACTGTCCACCCAGCGTTCGATATATGAC                                                                                                                   <  1:230246/151‑1                                                       CGATGGCCATCGGGGGTGGGCTGGTACATCCGATTTTTATTGACCTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCGGGTCACCGCCGGTGTAAT‑‑GCTGTCCACCCAGCGTTCGATATATGACAGGCACCAGG                                                                                                         <  2:1165718/150‑1                                                                                         TTTTATTAACCTGATGGCGAAGGCGGCCAAGGGTGAACATGTCGAATTTCCCCTGATTCGGTTCACCGCCGGTGTAAT‑‑GCTGTCCACCCAGCGTTCGATATATGACAGGCACCAGGTCATCACCAGCGCCGGGATCACCGTGTAGGTGTAT                                                                      <  2:1126078/151‑1                                                                                                GAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGCCGGTGTAAT‑‑GCTGTCCACCCAGCGTTCGATATATGACAGGCACCAGGTCATCACCAGCGCCGGGATCACCGTGTAGGTGTATTTCACCG                                                               >  1:1031505/1‑151                                                                                                                                                        CACCGCCGGTGTAAT‑‑GCTGTCCACCCAGCGTTCGATATATGACAGGCACCAGGTCATCACCAGCGCCGGGATCACCGTGTAGGTGTATTTCACCGCGGTGACCGGAATCAGGGCAAATTCGACATGTTCACCCTGGGCCGCTTTCGCCATC       <  1:550230/151‑1                                                                                                                                                             CCGGTGTAAT‑‑GCTGTCCACCCAGCGTTCGATATATGACAGGCACCAGGTCATCACCAGCGCCGGGATCACCGTGTAGGTGTATTTCACCGCGGTGACCGGAATCAGGGCAAATTCGACATGTTCACCCTGGGCCGCTTTCGCCATCAGTTC  <  1:314912/151‑1                                                                                                                                                                                                                                                                                                                       CGCTGATGGTCGCGGCATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCGGTCACCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2840008‑2840164 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggtcaccgcCGGTGTAAT‑‑GCTGTCCACCCAGCGTTCGATATATGACAGGCACCAGGTCATCACCAGCGCCGGGATCACCGTGTAGGTGTATTTCACCGCGGTGACCGGAATCAGGGCAAATTCGACATGTTCACCCTGGGCCGCTTTCGCCATCAGTTC  <  NC_000913/2840252‑2840103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.