Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,554,0000TG45.0% 55.8 / 10.1 61Y557S (TAC→TCC) maeAmalate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme
Reads supporting (aligned to +/- strand):  ref base T (15/18);  new base G (27/0);  total (43/18)
Fisher's exact test for biased strand distribution p-value = 1.43e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTAC  >  NC_000913/1553858‑1554148
                                                                                                                                              |                                                                                                                                                    
atgTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGc                                                                                                                                               <  1:1129645/150‑1 (MQ=255)
atgTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTTgg                                                                                                                                                >  1:1168816/1‑149 (MQ=255)
     cTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGGAGTTGCCGGATTCGGCTTGcg                                                                                                                                          >  2:1084527/1‑149 (MQ=255)
          tAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGGAGTCGGGGGATTCGGCTTGCCAGAAAt                                                                                                                                    >  1:683630/1‑151 (MQ=255)
               ggTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTAttg                                                                                                                               >  1:599469/1‑149 (MQ=255)
                   aGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCa                                                                                                                            <  2:72509/150‑1 (MQ=255)
                     gCAAAGGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAAt                                                                                                                          <  2:328373/150‑1 (MQ=255)
                      cAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATgg                                                                                                                        <  1:729190/151‑1 (MQ=255)
                        aaTGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCCTGCCAGAAATTATCGTCAATGGc                                                                                                                       >  1:703243/1‑150 (MQ=255)
                             gaTTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGGCCGAAATTATCGTCCATGGCCTGGTg                                                                                                                 >  2:324527/1‑151 (MQ=255)
                                  aTTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTGCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAAGGGCCTGTTGCAggg                                                                                                            <  2:49951/151‑1 (MQ=255)
                                   ttCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAATTAGCCCGGTAGCCTTCACTACTGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGc                                                                                                           <  2:1171715/151‑1 (MQ=255)
                                       ccACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTTCAGGGCTTCg                                                                                                       >  1:680714/1‑151 (MQ=255)
                                       ccACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAAAGGCCTGTTGCAGGGCTTCg                                                                                                       >  1:178117/1‑151 (MQ=255)
                                            aCCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCCGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCaga                                                                                                  >  1:95031/1‑151 (MQ=255)
                                            aCCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGAGCCGGAATTTGGGCTGCCAGAAATTATGGTCGATTGGCCGTTGTGGGGGTTCGGctgg                                                                                                  >  1:82323/1‑148 (MQ=255)
                                               cAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGCAGTCGCGGGATTCGGCGTGGCCGAAAATTTCGGCAATGGACTGTTGCAGGGCTTTGGCAGAGGt                                                                                               >  2:972769/1‑151 (MQ=255)
                                                 gTATGGATGAGATCTGAACAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGAGTGGCAGAAATTATTCTCCCCGGCGTGTTTCAAGGGTTCGGCCGGGGttt                                                                                             >  2:858977/1‑151 (MQ=255)
                                                 gTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTTTGCTTTCCCGAAATTTTCCTCAAAGGCCTTTTTCAGGGCCTCGGCAGAGGgtt                                                                                             >  1:1119614/1‑151 (MQ=255)
                                                 gTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTTCCAGAAATTATCGTCAATGGCCTGTTGCAGGGGTTCGGCAGAGGgtt                                                                                             >  1:711515/1‑151 (MQ=255)
                                                 gTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGgtt                                                                                             >  1:779958/1‑151 (MQ=255)
                                                 gTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGgtt                                                                                             >  1:701615/1‑151 (MQ=255)
                                                 gTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGGGGCGGGATTCGGCCTGCCAGAAATTATAGTTAATGGGCCGCTGCAggggctctggcgggggtt                                                                                             >  2:1110076/1‑136 (MQ=255)
                                                 gTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGGAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTTCAGGGGTTCGGGAGAGGgtt                                                                                             >  1:641573/1‑151 (MQ=255)
                                                      gATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGGCTGTTGGAGGGCTTCGGGGGAGGGTTTTAc                                                                                         >  1:400609/1‑150 (MQ=255)
                                                      gATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCAc                                                                                         <  2:95003/150‑1 (MQ=255)
                                                      gATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCAc                                                                                         <  2:528456/150‑1 (MQ=255)
                                                      gATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCCCGGGATTCGGCTTGCCAGAAATTATTGTCAATGGCCCGTTGCAGGGCTTCGGCAGAGGGTTTCAc                                                                                         >  1:356351/1‑150 (MQ=255)
                                                      gATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAATCGGGGTATTCGGCCTGCCAGAAATTATCGTCAAAGGCCTGGTGGAGGGCTTCGGGAGAGGGTTTTAc                                                                                         >  1:990685/1‑150 (MQ=255)
                                                      gATGAGATCGGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCAc                                                                                         <  2:688447/150‑1 (MQ=255)
                                                           gaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGCACGGCGGTCGCTGCCGTATTTGGCTTGCCCGCAAATATCGCCCATCGGCTCTTGCAGGGCCTCCGCAGAGGGCTTCTTCGCCa                                                                                    >  2:362606/1‑150 (MQ=255)
                                                               tGAAAAAGGGAGAGGAAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGAGGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTACCGTAAATGGCCTTTTGCAGGGTTTCGGCAGAGGTTTTCACCGCCACGCCt                                                                               <  2:932726/151‑1 (MQ=255)
                                                                gAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGGCAGAAAATATCGTCAAAGGCCTGTTGCCGGGCTTCGGCAGAGGTTTTCACCGCCACGcc                                                                                >  2:279637/1‑149 (MQ=255)
                                                                   aaaGGGAGAGGAAAATAGCCCGGTAGCCTCCACTACCGGGCGCAGGCTTAGATGGAGGGACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTtgct                                                                           <  2:711181/151‑1 (MQ=255)
                                                                       ggAGAGGGAAATCCCCCGGTAGCCTGCCCGCCCGGGCGAAGGCTTAGATGGAGGTAGGGCGGTGGTCGCGGTATTCGGCTTGCAAGAAATTATCGTAAAGGCCTTGTTCAAGGTGTTCGGCAGGGTTTTTCACCGCCACGCCTtgctgc                                                                         <  1:852020/149‑1 (MQ=255)
                                                                            gggAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGGTTGCCCGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTCACCGCCACGCCTTGCTGCTGCGcc                                                                   >  2:59872/1‑150 (MQ=255)
                                                                             ggAAATAGCCCGGTAGCCTGAACTCCCGGGCGCAGGCTTAGATGGAGGTAAGGCGGTTGGCGCCGGATTCCGCTTTGCCGAAAATAACTTTAAAGGGCTGGCGGCGGGTCTCGGCAGAGGGTTTCACCGCCCGGGCTACCTCCTGCGcc                                                                   >  2:682943/1‑149 (MQ=255)
                                                                               aaaTAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTCACCGCCCCCCCTTGCTGCTGCGCCCttt                                                               >  1:147689/1‑151 (MQ=255)
                                                                                         gTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTTACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCg                                                      >  1:273450/1‑150 (MQ=255)
                                                                                            gCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTTGGCCTGCCAGAAATTATCGTTCAAGGCCTGTTGGAGGGCTTCGGCAGAGGGTTTCACCGCCCCGCCCTGCTGCTGGGCCCTTTTGCCCACCGCcaa                                                  >  2:382746/1‑151 (MQ=255)
                                                                                              cTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAAAGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCCTGCTGCTGCGCCATTTTGCCAACCGCCAACg                                                >  1:750823/1‑151 (MQ=255)
                                                                                                tCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAAATATCGTCAATGGTCCGTTGCAGGGCTTCGGCCGAGGGTTTCACCGGCCCGCCCTGCTGCTGGGCCATTTTTCCAACCGCAAAAGCa                                              >  2:7640/1‑151 (MQ=255)
                                                                                                     cccGGGCGCAGGCTTAGATGGGGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCGGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTgc                                         <  1:105716/150‑1 (MQ=255)
                                                                                                               ggCTTAGATGGAGGTACGGCGGTATCAGCGGGATTCCGGTTGCCACCAATTATCGGCAAAGGGCTGTTGCAGGGCTTCGGCAGAGGGTTTTACCGCCCCGCCCTGGTGCCGCGCCATTTTGCCCAACGCACACGCAATTGCGCCGgggaat                               >  2:1100243/1‑149 (MQ=255)
                                                                                                               ggCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCTGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTTCCAACCGCAAACGCAATTGCGCGGgaga                                 >  1:470433/1‑149 (MQ=255)
                                                                                                               ggCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTCCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGggga                                 >  1:182545/1‑149 (MQ=255)
                                                                                                               ggCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAATGGGCTGTTGCAGGGCTTCGGCAGAGGGTTTTACCGCCACGCCTTGCTGCTGCGCCATTTTTCCAAACGCAAAAGCAATTGCGCGGggga                                 >  1:336679/1‑149 (MQ=255)
                                                                                                               ggCTTAGATGGAGGTACGGCGGTAGTCGCGGGATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTCCCCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCCCAAACGCAATTGCGCGGggga                                 >  1:538605/1‑149 (MQ=255)
                                                                                                                gtttAGATGGAGGTCCGGCGGTAGTCGCGGTATTCGGCTTGCCAAAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGGGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACt                               <  1:718820/148‑1 (MQ=255)
                                                                                                                gCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTTTCGTCAATGGCCTGTTGCCGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCCTTTTTCCAACCGCAAACGCAAATGCGCGGGGGACtt                              >  1:560765/1‑151 (MQ=255)
                                                                                                                           ggTACGGCGGTAGTCGCGGGATTCGGCTTGCCCGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTTACCGCCACGCCCTGCTGCTGCGCCCTTTTTCCAAACCCCAAAGCAATTTCGCGGGGGACTTTTTTAatattt                   >  1:326520/1‑149 (MQ=255)
                                                                                                                             tACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCtt                  <  1:655628/150‑1 (MQ=255)
                                                                                                                                 gcggTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGt             >  2:272573/1‑151 (MQ=255)
                                                                                                                                  cggTAGTCGGGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGTTTCGGAAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGt             <  2:300204/150‑1 (MQ=255)
                                                                                                                                    gTAGTGGCGGTATTCGGCTTGCCAGAAATTATCGTAAATGCCCGGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTc           <  1:859347/150‑1 (MQ=255)
                                                                                                                                    gTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTc           >  2:554997/1‑150 (MQ=255)
                                                                                                                                             gTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTAc  >  1:172061/1‑150 (MQ=255)
                                                                                                                                             gTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTAc  >  1:933659/1‑150 (MQ=255)
                                                                                                                                             gTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTAc  >  1:1065835/1‑150 (MQ=255)
                                                                                                                                             gTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTAc  >  1:443152/1‑150 (MQ=255)
                                                                                                                                             gTATTCGGCTTGCCAGAAATTATCGTAAAGGGCCTTTTGCGGGGTTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTAc  <  2:750651/150‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
ATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTAC  >  NC_000913/1553858‑1554148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑