Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,072,171	C→A	V161F (GTC→TTC)	yihT ←	6‑deoxy‑6‑sulphofructose‑1‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,072,171	0	C	A	100.0%	245.0 / NA	67	V161F (GTC→TTC)	yihT	6‑deoxy‑6‑sulphofructose‑1‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/37);  total (30/37)

GCACAGAGAAGCTCTTGTTGCGGACCTTTGCCATAAAGCGGCATTTCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGACTGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGAT  >  NC_000913/4072023‑4072309                                                                                                                                                     |                                                                                                                                           gCACAGAGAAGCTCTTGTTGCGGACCTTTGCCATAAAGCGGCATTTCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATg                                                                                                                                          >  1:285903/1‑151 (MQ=255) gCACAGAGAAGCTCTTGTTGCGGACCTTTGCCATAAAGCGGCATTTCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATg                                                                                                                                          >  2:809765/1‑151 (MQ=255) gCACAGAGAACATCTTGTTGCCGACCTTTACCATAAAGCGCCATTTCAACTTTGTAGAGGTCAGCCCCACCGTCACCAAGCTCTTTGGCGGCCTCGATGATAGCATGTTCGCGCTCGACTTTATCGCACCGACGTGCTGGGCGGACGAATg                                                                                                                                          >  2:92402/1‑151 (MQ=255)    caGAGAAGCTCTTGTTGCGGACCTTTGCCATAAAGCGGCATTTCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATgg                                                                                                                                         >  1:930984/1‑149 (MQ=255)    caGAGAAGCTCTTGTTGCGGACCTTTGCCATAAAGCGGCATTTCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATgg                                                                                                                                         >  2:1172620/1‑149 (MQ=255)            ctctTGTTGCGGACCTTTGCCATAAAGCGGCATTTCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAatga                                                                                                                                 <  1:851076/149‑1 (MQ=255)            ctctTGTTGCGGACCTTTGCCATAAAGCGGCATTTCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAatga                                                                                                                                 <  2:878786/149‑1 (MQ=255)             tctTGTTGCGGACCTTTGCCATAAAGCGGCATTTCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGc                                                                                                                              <  2:1144631/151‑1 (MQ=255)                 gttgCGGACCTTTGCCATAAAGCGGCATTTCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTa                                                                                                                           >  1:1113573/1‑150 (MQ=255)                  ttgCGGACCTTTGCCATAAAGCGGCATTTCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTAcc                                                                                                                         <  2:1184323/151‑1 (MQ=255)                  ttgCGGACCTTTGCCATAAAGCGGCATTTCAACTTTGAATCGGTCACCGCCACTGTCGCCCAGCTCTTTGGCCGCATCGATGAACGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGCTGCTTAc                                                                                                                          >  2:446641/1‑150 (MQ=255)                   tgCGGGCCTGTGCCGTAAAGCGGCGTTTCAACATTGTAGAGGGCGGCACCGCTGTCGCACAGCCCTTGGGGGGGGTCGATGACCGCTTGTTCGCGATCGGAGTTGTCGCCACGACGCGGTGGGCGGACGAATGGGTTAATGATGTTTAcc                                                                                                                         <  2:566044/150‑1 (MQ=255)                       gACCTTTGCCATAAAGCGGCATTTCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGAc                                                                                                                     <  1:685315/150‑1 (MQ=255)                       gACCTTTGCCATAAAGCGGCATTTCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGAc                                                                                                                     <  2:151927/150‑1 (MQ=255)                              gCCATAAAGCGGCATTTCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAg                                                                                                             >  1:607647/1‑151 (MQ=255)                               ccATAAAGCGGCATTTCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGt                                                                                                            >  2:592711/1‑151 (MQ=255)                                 aTAAAGCGGCATTTCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCCGCTCTTTGGCGGCATCGAAGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCACTGATGCTTACCAGACCGTGTGAGTgg                                                                                                          >  1:952073/1‑151 (MQ=255)                                    aaGCGGCATTTCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGcac                                                                                                       <  1:736615/151‑1 (MQ=255)                                         gCATTTCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCCCAATGATGCTTACCAGACCGTGTGAGTGGCCCAGtt                                                                                                   >  1:520076/1‑150 (MQ=255)                                              tCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGa                                                                                             <  2:307074/151‑1 (MQ=255)                                              tCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGa                                                                                             <  2:91890/151‑1 (MQ=255)                                               cAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGaa                                                                                            <  1:227958/151‑1 (MQ=255)                                                aaCTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAAc                                                                                           <  1:430860/151‑1 (MQ=255)                                                 aCTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGaa                                                                                            >  1:27788/1‑149 (MQ=255)                                                    ttGTAAAGGTCAGCGCCACTCCCGCCCAGCCCTTTGGCGGCCTCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGCATGGCTCACTGATGCTTACCACACCGCGTGACTGGCACAGTTCGTTGAACTCtt                                                                                       >  2:625299/1‑151 (MQ=255)                                                      gTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAg                                                                                                             <  2:638641/127‑1 (MQ=255)                                                      gTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAg                                                                                                             >  1:638959/1‑127 (MQ=255)                                                      gTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCttt                                                                                      >  1:1121879/1‑150 (MQ=255)                                                          agGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTATTTCAcc                                                                                  <  2:930591/150‑1 (MQ=255)                                                            gTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCa                                                                                 <  2:117299/149‑1 (MQ=255)                                                              cAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCatat                                                                              >  1:274761/1‑150 (MQ=255)                                                                 cgcCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAGTGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATcc                                                                            <  2:116925/149‑1 (MQ=255)                                                                    cACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACg                                                                       <  2:41105/151‑1 (MQ=255)                                                                     aCTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGt                                                                      <  1:154022/151‑1 (MQ=255)                                                                         tCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCGCAGTTCGTTGAACTCTTTCACCATATCCAGACGttaa                                                                   <  2:388087/150‑3 (MQ=255)                                                                         tCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTtgc                                                                   <  1:232179/150‑1 (MQ=255)                                                                             ccAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTgcgc                                                              <  1:468601/151‑1 (MQ=255)                                                                                gCTCTTTGGCGTCATCGATGATCCCTTGTTCACCATAGAATTTAGCGCCACTTCGCGGTGTGCGGACGAATGGCGCAATGATGCTTACCAGACAGTGTGTGTCGCATACCGCGATGAACTCTTTCACCATATCCAGACTTCGCTGCGCAt                                                            >  2:664177/1‑150 (MQ=255)                                                                                gCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTgcggcga                                                           >  1:177238/1‑147 (MQ=255)                                                                                gCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCAt                                                            >  1:686288/1‑150 (MQ=255)                                                                                gCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCAt                                                            >  2:578481/1‑150 (MQ=255)                                                                                gCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCAt                                                            >  2:1157850/1‑150 (MQ=255)                                                                                   ctTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTtc                                                        <  1:547682/151‑1 (MQ=255)                                                                                   ctTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTtc                                                        <  1:930498/151‑1 (MQ=255)                                                                                              tCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCACTGATGCTTACCAGACCGTGTGAGTGGCAAAGGTCGTTGAACTATTTCACCATATCCAAACGTTGCTGCGCATGTTCCTCACTACGc                                              <  2:1043190/150‑1 (MQ=255)                                                                                                gatgatCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCca                                            >  1:467350/1‑150 (MQ=255)                                                                                                 atgatCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCcac                                           <  2:1056193/150‑1 (MQ=255)                                                                                                  tgatCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAg                                         <  1:872200/151‑1 (MQ=255)                                                                                                     tCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCAc                                      <  1:770489/151‑1 (MQ=255)                                                                                                          tGTTCGCGATCGAATTTATCGCCACGTCGCGGTGGGCGGACGAATGGCTCAATGATGCTACCAAGACCGTGTGAGTGGCACGGTTCGTTGAACTCTTTCACCAGATCCAGACGTTGCTGCACCTCTTCATCACTACGCCACAGCACcagca                                 <  2:36531/151‑1 (MQ=255)                                                                                                            ttCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACcagcag                                <  1:517936/150‑1 (MQ=255)                                                                                                                cgATCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTa                           >  2:1121403/1‑151 (MQ=255)                                                                                                                  aTCGAATTTATCGCCACGACGCGGAGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAg                         >  1:32513/1‑151 (MQ=255)                                                                                                                  aTCGAAGTTATCGCCACGACGCGGTGGTCTGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACACTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAg                         >  2:463133/1‑151 (MQ=255)                                                                                                                   tCGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAgg                        <  2:1051809/151‑1 (MQ=255)                                                                                                                    cGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGc                       <  1:593008/151‑1 (MQ=255)                                                                                                                    cGAATTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGc                       <  1:832951/151‑1 (MQ=255)                                                                                                                      aaTTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCtt                     >  1:320017/1‑151 (MQ=255)                                                                                                                       aTTTATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCttt                    >  1:593248/1‑151 (MQ=255)                                                                                                                          tATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGcc                 <  1:294558/151‑1 (MQ=255)                                                                                                                          tATCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGcc                 <  2:1077717/151‑1 (MQ=255)                                                                                                                            tCGCCACGACGCGGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCAc               <  2:374012/151‑1 (MQ=255)                                                                                                                                 acgacgCGGTGGGCGGACGAATGGCCCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGGTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTCTGCCACCGt            >  2:1169995/1‑149 (MQ=255)                                                                                                                                    acgCGGTGGGCGGACGAATGGCTCAATGATGCTTGCCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTg        <  1:593985/150‑1 (MQ=255)                                                                                                                                       cgGTGGGCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGTCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGttt     <  1:1169246/150‑1 (MQ=255)                                                                                                                                           gggCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGAt  >  1:614915/1‑149 (MQ=255)                                                                                                                                           gggCGGACGAATGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGAt  >  1:76732/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                           GCACAGAGAAGCTCTTGTTGCGGACCTTTGCCATAAAGCGGCATTTCAACTTTGTAGAGGTCAGCGCCACTGTCGCCCAGCTCTTTGGCGGCATCGATGATCGCTTGTTCGCGATCGAATTTATCGCCACGACGCGGTGGGCGGACGACTGGCTCAATGATGCTTACCAGACCGTGTGAGTGGCACAGTTCGTTGAACTCTTTCACCATATCCAGACGTTGCTGCGCATCTTCATCACTACGCCACAGCACCAGCAGTTTTAAGGCTTTGCCACCGTCTTGTTTGAT  >  NC_000913/4072023‑4072309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑