Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,750,720	C→T	intergenic (‑12/‑111)	grpE ← / → nadK	heat shock protein/NAD kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,750,720	0	C	T	100.0%	282.4 / NA	75	intergenic (‑12/‑111)	grpE/nadK	heat shock protein/NAD kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/47);  total (28/47)

GCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGAC  >  NC_000913/2750589‑2750867                                                                                                                                    |                                                                                                                                                    gcgcgGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCAt                                                                                                                                    >  1:635119/1‑149 (MQ=255)  cgcgGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCAtct                                                                                                                                  <  1:1103456/150‑1 (MQ=255)    cgGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCg                                                                                                                                >  1:1074701/1‑150 (MQ=255)    cgGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCg                                                                                                                                >  2:1097266/1‑150 (MQ=255)     ggATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCg                                                                                                                                >  2:249074/1‑149 (MQ=255)      gATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCt                                                                                                                              <  1:849874/150‑1 (MQ=255)      gATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTa                                                                                                                             >  2:629497/1‑151 (MQ=255)      gATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTa                                                                                                                             >  2:425789/1‑151 (MQ=255)        tCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAAc                                                                                                                           <  2:99922/151‑1 (MQ=255)          cACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACtt                                                                                                                         >  2:1179686/1‑151 (MQ=255)                  cagcagAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGCTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGcttctt                                                                                                                 >  2:189072/1‑151 (MQ=255)                     cagAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCttatta                                                                                                                <  1:998599/149‑1 (MQ=255)                     cagAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCttatt                                                                                                                 <  1:200221/148‑1 (MQ=255)                          gCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGAt                                                                                                         >  2:277877/1‑151 (MQ=255)                           cTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATc                                                                                                        <  2:14008/151‑1 (MQ=255)                           cTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATc                                                                                                        <  1:658793/151‑1 (MQ=255)                                     aaCTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAggg                                                                                              <  1:210426/151‑1 (MQ=255)                                     aaCTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAggg                                                                                              <  1:422869/151‑1 (MQ=255)                                     aaCTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAggg                                                                                              <  2:292073/151‑1 (MQ=255)                                     aaCTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAggg                                                                                              <  2:7855/151‑1 (MQ=255)                                             cAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCGCCTCAGGCGTTTTCTGGTCTTTACTACTCATGAAGCTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCaaag                                                                                      <  2:820355/151‑3 (MQ=255)                                             cAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAgg                                                                                      <  2:1143137/151‑1 (MQ=255)                                             cAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAgg                                                                                      <  2:228486/151‑1 (MQ=255)                                               aTCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGa                                                                                    >  1:200281/1‑151 (MQ=255)                                                 ctctTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAg                                                                                  <  2:341847/151‑1 (MQ=255)                                                      cGTGCTGATCCATGATAATTTCTTCCGTGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGGTGCAAGGGAAGCACTc                                                                             <  2:684176/151‑1 (MQ=255)                                                       gTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTca                                                                            >  1:816712/1‑151 (MQ=255)                                                       gTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTca                                                                            >  2:1039998/1‑151 (MQ=255)                                                          cTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTcaca                                                                          <  1:1140383/150‑1 (MQ=255)                                                          cTGATCCATGATAATTTCTTCCGGGGCTTGCCACTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTcaca                                                                          <  2:1121618/150‑1 (MQ=255)                                                            gATCCATGATAATTTCGTCCGGGGCTCGCCCCTCCGGCGTTTTCCGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTg                                                                       >  2:552892/1‑151 (MQ=255)                                                             aTCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTg                                                                       <  1:193169/150‑1 (MQ=255)                                                             aTCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTg                                                                       <  2:302837/150‑1 (MQ=255)                                                             aTCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTg                                                                       <  2:988989/150‑1 (MQ=255)                                                             aTCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTg                                                                       <  1:1133354/150‑1 (MQ=255)                                                                cATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGCATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGCGGATCAGTTTCAGGGTTTCAAGTGAAGCACTCACATTGtcat                                                                   >  2:1186852/1‑151 (MQ=255)                                                                  tGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGtcatca                                                                 >  1:648107/1‑151 (MQ=255)                                                                    ttAATTTCTTCCGGTGCTTGCCCCTCAGTCTCGTTCTGTTGTTTGCTACTCATTAATTGCTCCTTGTTGTTTTCGCATTCGTCTCGCTGACTTCGCTTATTATGGGGAGCAGGTTCAGGGTTTCAAGGGAAGCACTCACATTGGCATCAAt                                                               <  2:1017922/150‑1 (MQ=255)                                                                    aTAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAAt                                                               <  1:189125/151‑1 (MQ=255)                                                                     tAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATc                                                              <  2:608396/151‑1 (MQ=255)                                                                     tAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATc                                                              <  1:445367/151‑1 (MQ=255)                                                                      aaTTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAAt                                                               >  1:752628/1‑149 (MQ=255)                                                                           cttcCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGca                                                        >  2:1103828/1‑151 (MQ=255)                                                                           cttcCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGca                                                        <  2:1020135/151‑1 (MQ=255)                                                                             tcCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGcaac                                                      <  2:378376/151‑1 (MQ=255)                                                                                   gCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGa                                                 <  2:381287/150‑1 (MQ=255)                                                                                   gCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCCTCGCAACAAGGa                                                 <  1:765021/150‑1 (MQ=255)                                                                                    cTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGAc                                                <  1:100683/150‑1 (MQ=255)                                                                                    cTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGACATCAATCTTCGCAACAAgggc                                                <  2:848847/150‑3 (MQ=255)                                                                                        ccccTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCgg                                           >  1:512059/1‑151 (MQ=255)                                                                                         cccTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGa                                          <  1:748942/151‑1 (MQ=255)                                                                                               ggCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTcaca                                                                          <  1:196244/113‑1 (MQ=255)                                                                                               ggCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTcaca                                                                          >  2:196189/1‑113 (MQ=255)                                                                                                    tttCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTg                                                                       >  2:507302/1‑111 (MQ=255)                                                                                                    tttCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTg                                                                       <  1:507525/111‑1 (MQ=255)                                                                                                       cTGTTCTTGACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCGTCGCAACAAGGACCTCGGAAAAATGAATAAGCa                            <  2:1048116/151‑1 (MQ=255)                                                                                                         gTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCAtt                          >  1:658838/1‑151 (MQ=255)                                                                                                            cTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCACGGGAAGCCCTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCa                       >  1:353582/1‑151 (MQ=255)                                                                                                            cTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCa                       <  1:917163/151‑1 (MQ=255)                                                                                                            cTTTACTACTCATAACTTTCTCCGTGTTTTTTTCGAATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCCAGGGAATCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCa                       >  2:885422/1‑151 (MQ=255)                                                                                                              ttACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAg                     >  1:1250/1‑151 (MQ=255)                                                                                                                actactCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAgtg                   <  2:752291/151‑1 (MQ=255)                                                                                                                    ctCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTAtt               >  1:19743/1‑151 (MQ=255)                                                                                                                     tCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTg              <  2:79519/151‑1 (MQ=255)                                                                                                                      cATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTgg             >  2:543197/1‑151 (MQ=255)                                                                                                                      cATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTgg             >  1:446914/1‑151 (MQ=255)                                                                                                                       aTGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGc            <  1:928107/151‑1 (MQ=255)                                                                                                                       aTGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGc            <  1:535029/151‑1 (MQ=255)                                                                                                                        tGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCa           >  1:819248/1‑151 (MQ=255)                                                                                                                        tGAATTTCTCCGTGTTTTTGTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCa           <  2:130296/151‑1 (MQ=255)                                                                                                                          aaTTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCAt          <  1:5361/150‑1 (MQ=255)                                                                                                                          aaTTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCAt          <  1:301856/150‑1 (MQ=255)                                                                                                                          aaTTGCTCCGTGTATTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGCGGATCAGTTTCAGAGTTTCAAGGGAAGCACTCAAATTGTCATCAATCGTCGAAACAAGGACCTCAGAAAAACGAATCATAATTTCAAGTGTATTGGCAt          <  2:232589/150‑1 (MQ=255)                                                                                                                              tctcCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTgg     <  1:235829/151‑1 (MQ=255)                                                                                                                                 ccGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGCATTGGCATTGTGGGac  <  1:1180244/151‑1 (MQ=255)                                                                                                                                    |                                                                                                                                                    GCGCGGATCCACCTGCTCAGCAGAAGCTTCTGGCTCAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACAAGGACCTCGGAAAAATGAATAATCATTTCAAGTGTATTGGCATTGTGGGAC  >  NC_000913/2750589‑2750867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑