Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	701,381	(C)5→4	coding (214/1221 nt)	nagC ←	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	701,377	0	C	.	100.0%	245.2 / NA	56	coding (218/1221 nt)	nagC	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (32/24);  total (32/24)

GGGTACGTTCCGGCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAAT  >  NC_000913/701231‑701516                                                                                                                                                   |                                                                                                                                            gggTACGTTCCGGCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGccccg                                                                                                                                         >  1:861409/1‑150 (MQ=255) gggTACGTTCCGGCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGccccg                                                                                                                                         >  2:289979/1‑150 (MQ=255)  ggTACGTTCCGGCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGGGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGccccgg                                                                                                                                        >  2:1050140/1‑149 (MQ=255)    tACGTTCCGGCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGccccggtg                                                                                                                                      <  2:973703/151‑5 (MQ=255)      cGTTCCGGCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGa                                                                                                                                   >  1:931325/1‑151 (MQ=255)         tCCGGCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGc                                                                                                                                <  2:225455/151‑1 (MQ=255)         tCCGGCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGc                                                                                                                                <  1:360915/151‑1 (MQ=255)          ccGGCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGcc                                                                                                                               <  2:183802/151‑1 (MQ=255)                   ggTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATc                                                                                                                       <  1:620523/150‑1 (MQ=255)                   ggTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCa                                                                                                                      >  1:713042/1‑151 (MQ=255)                       aTGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAActt                                                                                                                  >  2:341975/1‑151 (MQ=255)                               ctGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGAt                                                                                                           >  2:293466/1‑150 (MQ=255)                               ctGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATc                                                                                                          <  1:696985/151‑1 (MQ=255)                               ctGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATc                                                                                                          <  1:650502/151‑1 (MQ=255)                                 gCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAg                                                                                                        >  1:397676/1‑151 (MQ=255)                                 gCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAg                                                                                                        >  2:765274/1‑151 (MQ=255)                                        cTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGt                                                                                                 >  2:837276/1‑151 (MQ=255)                                                 tgAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGc                                                                                        <  1:783361/151‑1 (MQ=255)                                                  gagaTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAg                                                                                         >  2:388494/1‑149 (MQ=255)                                                  gagaTCAAACAGAGTGATGGGGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAg                                                                                         >  1:850203/1‑149 (MQ=255)                                                   agaTCAAGCAGAGTGATGGGGGCGTCATGACGACAAAGACGTACGCCGATTGCGTGGAAATGGCGGTTTTCGGTGAGGATGAAGAGAGCGCGGCGG‑CCCCGGTGGGGGCCTGCTGATCAGCTTCTTTGATCAGCCCGCGTTCGAGAAggt                                                                                       <  1:12887/150‑3 (MQ=255)                                                   agaTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAg                                                                                         >  1:63417/1‑148 (MQ=255)                                                   agaTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGGTTCGGTGACGATGGAGATAGCGCGGCGG‑GCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCt                                                                                       <  1:533314/150‑1 (MQ=255)                                                    gaTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGa                                                                                     >  2:837446/1‑151 (MQ=255)                                                    gaTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGa                                                                                     >  2:807747/1‑151 (MQ=255)                                                    gaTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGa                                                                                     >  1:505517/1‑151 (MQ=255)                                                     aTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGa                                                                                     >  1:627654/1‑150 (MQ=255)                                                     aTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGa                                                                                     >  1:851119/1‑150 (MQ=255)                                                             gagTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAAtt                                                                            >  1:516347/1‑151 (MQ=255)                                                             gagTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAAtt                                                                            >  1:311582/1‑151 (MQ=255)                                                             gagTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAAtt                                                                            >  2:1020125/1‑151 (MQ=255)                                                               gTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAAtttt                                                                          >  1:37719/1‑151 (MQ=255)                                                                  aTGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGt                                                                       >  2:744322/1‑151 (MQ=255)                                                                  aTGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGt                                                                       >  1:444624/1‑151 (MQ=255)                                                                            cATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCg                                                             >  1:957988/1‑151 (MQ=255)                                                                            cATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCg                                                             >  1:1044368/1‑151 (MQ=255)                                                                                 cgacCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTACTTTTGGTTACGCTGGCggggg                                                         >  2:780360/1‑150 (MQ=255)                                                                                      aaGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCt                                                   <  1:18412/151‑1 (MQ=255)                                                                                       aGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAg                                                     >  1:508248/1‑148 (MQ=255)                                                                                        gCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTgg                                                 >  2:561638/1‑151 (MQ=255)                                                                                        gCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTgg                                                 >  2:255035/1‑151 (MQ=255)                                                                                        gCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTgg                                                 >  2:271402/1‑151 (MQ=255)                                                                                        gCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGAGCAACTTCTTTGATCAGACCGCGTTCGATAAGCTGACGCGTAATTTTTGTTACGCTGGCCGCGGCAAGCTgg                                                 >  1:763009/1‑151 (MQ=255)                                                                                            tACGCCGATTGCGTAGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAGCTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTGCGCTGGCGGGGGGAAGTTGGCtt                                              <  1:743958/150‑1 (MQ=255)                                                                                              cGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATTAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCCAGCTGGCTTTg                                            >  2:982110/1‑150 (MQ=255)                                                                                                ccGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCt                                          <  1:1053679/150‑1 (MQ=255)                                                                                                            aaaTTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGaa                              <  2:444791/150‑1 (MQ=255)                                                                                                                      tttCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGAtt                   >  1:213448/1‑151 (MQ=255)                                                                                                                      tttCGGTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTGGATCAGCCCGCGTTCGATAAGCTGGCGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGAt                    <  2:713300/150‑1 (MQ=255)                                                                                                                          ggTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGc                >  1:344533/1‑150 (MQ=255)                                                                                                                           gTGACGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGcc               <  2:657933/150‑1 (MQ=255)                                                                                                                               cGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTAc          <  1:834882/151‑1 (MQ=255)                                                                                                                               cGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTAc          <  1:723757/151‑1 (MQ=255)                                                                                                                               cGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTAc          <  1:435298/151‑1 (MQ=255)                                                                                                                               cGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTAc          <  1:229783/151‑1 (MQ=255)                                                                                                                               cGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTAc          <  2:907223/151‑1 (MQ=255)                                                                                                                               cGATGGAGATAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTAc          <  1:118016/151‑1 (MQ=255)                                                                                                                                     agaTAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTc     <  2:710257/150‑1 (MQ=255)                                                                                                                                     agaTAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGAGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTc     <  1:435370/150‑1 (MQ=255)                                                                                                                                       aTAGCGCGGCGG‑CCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAAt  <  1:222332/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                            GGGTACGTTCCGGCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAAT  >  NC_000913/701231‑701516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑