Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,556,207 | 0 | T | C | 55.4% | 14.0 / 75.9 | 56 | S215G (AGT→GGT) | rtcA | RNA 3'‑terminal phosphate cyclase |
Reads supporting (aligned to +/- strand): ref base T (24/1); new base C (0/31); total (24/32) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.74e-15 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-08 |
GCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCG‑ACTTCAAGCGAGACGGTATT‑ACCCGG‑CCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCA > NC_000913/3556059‑3556350 | gCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCG‑ACTTCAAGCGAGACGGTATT‑ACCCGG‑CCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTc > 1:805618/1‑150 (MQ=255) ccTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCG‑ACTTCAAGCGAGACGGTATT‑ACCCGG‑CCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAgtgt > 2:489773/1‑151 (MQ=255) cGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCG‑ACTTCAAGCGAGACGGTATT‑ACCCGG‑CCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAg > 1:739584/1‑150 (MQ=255) gcTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCG‑ACTTCAAGCGAGACGGTATT‑ACCCGG‑CCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCt > 1:429762/1‑150 (MQ=255) tccccgagcgaaAAAAGAGGGTCCGTTAATATTTCCACTTGCG‑ACTACAGGCGAGACGTTATT‑‑CCCCG‑CCCCTGGTCCCCGCGGGGGTTTAGAAATTTTCTGTTC‑ATGCGGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCa < 1:580640/143‑1 (MQ=255) accgccaaaaaaaagGCTTTGGGTAATATTTTCATGTGCG‑ATGGAAAGAGAGAGGTTTTT‑CCCCGC‑CCCCGGGC‑CGCGCGGCTGGTTATAAAAATTCTGTAC‑AGGCGGGGAAAA‑T‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACa < 2:200865/142‑1 (MQ=255) aaaaGCGTGGGGTAATTTTTTC‑CTTTCGAACTTCAAGCGAGCCGTTTTC‑CCCCGC‑CCCCTGTC‑CCCGGGGCAGTTTAAAAATATTCTTTTC‑AGGCAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcac < 1:1018845/151‑1 (MQ=255) tgtggtttttttttcCACTTTCG‑ACTTAAGGCGAG‑CGGTTTTCCCCCGC‑CCCCGGGC‑CGCGCGGCAGGTTAGAATTTTTCTGTCC‑AGGAGGGGAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGc < 1:132735/140‑1 (MQ=255) gTGATTTTTCCCCTTGCG‑ACTTCAAGCGAGACGTTATC‑ACCCGG‑CCCCTGGC‑CGCGCGGCAGGTTATAAATTTTCTGTTC‑ATGAAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAAt < 1:1031346/150‑1 (MQ=255) gTGATATTTTCACTTTCG‑ACTTCAAGCGAGACGGTATT‑ACCCGG‑CCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATa > 2:28408/1‑151 (MQ=255) ttatatTTCCCCTTTCG‑ACTTCAAGCGAGACGTTATT‑CCCCGG‑CCCCTGTC‑CGCGCGGCAGTTTAGAAATTTTCTGTTC‑AGGAAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAg < 1:696074/149‑1 (MQ=255) tGATTTTTTCTCTTGCG‑ACTTCAAGCGAGACGTTATT‑CCCCGC‑CCCCTGGT‑CGCGGGGCAGGTTATGAATTTTCTGTTC‑ATGCAGGAAAAA‑A‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAg < 1:608005/151‑1 (MQ=255) aattttttCGCTTGCG‑TCTACAAGCAGGACGTTTTT‑CCCGGC‑CCCCGGGT‑CGGGCGGCAGTTTATGAATTTTCTGTTC‑AGGGAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGa < 1:47712/147‑1 (MQ=255) atatTTTCACTTTCG‑ACTTCAAGCGAGACGGTATT‑ACCCGG‑CCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGaa > 2:866448/1‑151 (MQ=255) tttttccccTTTCC‑ACTTAAGGCGAGGCGTTTTT‑CCCCGC‑CCCCTGGC‑CGCGGGGCAGTTTATGAATTTTCT‑TTT‑AAGGAGGGAAAA‑ACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc < 1:489568/143‑1 (MQ=255) tatTTTCACTTTCG‑ACTTCAAGCGAGACGGTATT‑ACCCGG‑CCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcac > 1:373475/1‑136 (MQ=255) tttCACTTTCG‑ACTTCAAGCGAGACGGTATT‑ACCCGG‑CCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCCCAGCAACATGGCGCGGCACACCAGCTAATAGAACtt > 1:947572/1‑150 (MQ=255) tccG‑ACTTAAAGGGAGTCGTTTTT‑CCCCGG‑CCCCTGGG‑CCCGCGGCAGTTTAGAAATTTTTTGTCC‑AAGCGGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGc < 2:186454/149‑1 (MQ=255) tCG‑ACTTAAAGAGAGACGGTATT‑CCCCGG‑CCCCGGGG‑CGCGGGGCAGTTTATAAATATTCTGTTC‑ATGCGGGGAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCa < 2:44045/151‑1 (MQ=255) ttttaaagagggagggTATG‑CCCGGG‑CGCCGGGG‑CGCGGGGCGGGTTCAGCTTCTTCTGTTC‑ATGGGGGGAAAA‑A‑CCCCCCGCCAGTGTAGCGCTTTCCCGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCa < 2:389532/134‑1 (MQ=255) ttCAAGCGAGGCGGTATC‑CCCCGC‑CCCCGGGT‑CGCGGGGCGGTTTTAGATTATTCTGTTC‑AGGCAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGc < 2:629559/151‑1 (MQ=255) aaagggagggggTTTT‑CCCCGG‑CCCCGGGC‑CGCGCGGCAGGTTATGAATTTTCTGTCC‑AGGCAGGGAAAA‑A‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcac < 2:495450/141‑1 (MQ=255) gCGAGAGGGTTTT‑CCCCGC‑CCCCCGGC‑CGCGCGGCAGTTTTAGAATTTTCTGTTC‑ATCCAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACaa < 2:514584/150‑1 (MQ=255) agcgggTATT‑CCCCGG‑CCCCTGCC‑CGCGCGGCGGTTTATAAATATTCTGTTC‑ATGCAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt < 1:407039/146‑1 (MQ=255) agccgttATT‑CCCCGC‑CCCTTGGC‑CGCGCGGCAGTTTATGAATATTCTGTTC‑ATGCAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt < 1:1000593/144‑1 (MQ=255) agagGGTATC‑CCCCGC‑CCCCGGGC‑CGCGCGGAGGGTTATAAATATTCTGTCC‑AGGAAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt < 1:994573/150‑1 (MQ=255) acaCGGTATT‑CCCCGG‑CCCCTGGT‑CGCGCGGCGGTTTATAATTTTTCTGTTC‑ATGCAGGGAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt < 1:523743/148‑1 (MQ=255) gTATT‑AGCTGG‑CCCCTGGT‑CTCGCGGCAGGTTATGAATATTCTGTTC‑ATGCAGGGCAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg > 1:352773/1‑150 (MQ=255) gTATT‑ACCCGG‑CCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACCCCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg > 1:578864/1‑150 (MQ=255) gTATT‑ACCCGG‑CCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg > 2:13543/1‑150 (MQ=255) gTATT‑ACCCGG‑CCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg > 1:1019294/1‑150 (MQ=255) tATG‑ACCCGG‑CCACTTGT‑CCCCGGGCTAGTTATTAATTTTCTTTTC‑CAGGGGGGAAAACC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg < 1:596814/150‑1 (MQ=255) gtacaCCCGGCCCCCGGGG‑CCCGCGGCAGTTAAAAAAAATTCTGTCC‑AGGCGGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCCCGCCAGCTAATAGAGCTTCTCCACGCATCTGCACAATGTTCCcg < 2:449216/146‑1 (MQ=255) aCCCGG‑CCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTC‑ATCCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTc > 1:489931/1‑150 (MQ=255) tccccAGGT‑CGCGCGGCAGGTTATGAATATCCTGGTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg > 2:9382/2‑151 (MQ=255) ccccTGGC‑CGCGCGGC‑GGTTAATAAATTTTTGGTC‑CAGGAGGGAAAA‑ACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGt < 2:455420/151‑1 (MQ=255) cggtttttaaaTTTTCTGTTC‑AGGAAGGAAAAA‑A‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAc < 2:858459/142‑1 (MQ=255) ggtttttaaaTATTCTTTTC‑ATG‑AGGAAAAA‑ACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAc < 2:435447/142‑1 (MQ=255) ggtttATGAATTTTC‑GTTCAAGGGAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAc < 2:1034521/147‑1 (MQ=255) gggTTATAAATATTCTTTTC‑ATGCAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAc < 2:70765/149‑1 (MQ=255) ggTTATGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa > 2:198999/1‑151 (MQ=255) tttATGAATTTTCTTTTC‑ATGCGGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACg < 2:255692/148‑1 (MQ=255) tttATAAATTTTCTTTTC‑GTGCAGGGAAAA‑A‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa < 2:935728/149‑1 (MQ=255) gTTATGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt > 1:306774/1‑151 (MQ=255) gTTATGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt > 2:874323/1‑151 (MQ=255) gTTATGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt > 1:613418/1‑151 (MQ=255) gTTAAGAATTTTCTGTTC‑AGGGGGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAc < 2:646268/148‑1 (MQ=255) ttATGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATg > 1:995941/1‑151 (MQ=255) aTGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGc > 2:285899/1‑150 (MQ=255) tGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa > 2:810597/1‑151 (MQ=255) atatTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACgg > 2:528287/1‑151 (MQ=255) ttccaggaaGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGAc < 1:942303/143‑1 (MQ=255) tcc‑ATGCAGGAAAAA‑A‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGAc < 1:323467/148‑1 (MQ=255) gCAGGAAAAA‑C‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCg < 1:809516/150‑1 (MQ=255) gAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGGTGCCACGGGCGAGACTTCCGTTGcca > 2:863432/1‑151 (MQ=255) gAAAA‑A‑CTCCACGCCAGTGTAGCGATTTCACGCTAAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTTCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGcca > 2:829118/1‑151 (MQ=255) | GCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCG‑ACTTCAAGCGAGACGGTATT‑ACCCGG‑CCCCTGGT‑CGCGCGGCAGGTTATGAATATTCTGTTC‑ATGCAGGGAAAA‑A‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCA > NC_000913/3556059‑3556350 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |