Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	949,526	C→T	A15T (GCC→ACC)	ycaN ←	LysR family putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	949,526	0	C	T	100.0%	248.9 / NA	69	A15T (GCC→ACC)	ycaN	LysR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/36);  total (33/36)

GAACCCGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGCCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCGTAAAGTGCGCTCCAGAACTTAACGTGGAGGTAAAATTATGC  >  NC_000913/949378‑949671                                                                                                                                                     |                                                                                                                                                  gAACCCGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCa                                                                                                                                                 >  2:537928/1‑151 (MQ=255)     ccGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGc                                                                                                                                             <  2:460972/151‑1 (MQ=255)          tCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCaaaga                                                                                                                                        <  2:53015/151‑1 (MQ=255)           ccGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCaaagaa                                                                                                                                       >  1:655116/1‑151 (MQ=255)           ccGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCaaagaa                                                                                                                                       <  1:291814/151‑1 (MQ=255)             gTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCaaagaaag                                                                                                                                     >  2:900814/1‑151 (MQ=255)                 aCGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTgg                                                                                                                                  >  1:122388/1‑150 (MQ=255)                        gCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCa                                                                                                                          >  1:629432/1‑151 (MQ=255)                            cGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACa                                                                                                                      <  1:969317/151‑1 (MQ=255)                            cGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACa                                                                                                                      <  1:190446/151‑1 (MQ=255)                            cGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACa                                                                                                                      <  1:414548/151‑1 (MQ=255)                                 tGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTc                                                                                                                 <  1:994907/151‑1 (MQ=255)                                  gTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCa                                                                                                                >  2:907926/1‑151 (MQ=255)                                  gTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCa                                                                                                                >  1:36157/1‑151 (MQ=255)                                   tCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCa                                                                                                                <  1:956838/150‑1 (MQ=255)                                    ccGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATc                                                                                                              <  2:508060/151‑1 (MQ=255)                                     cGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATc                                                                                                              >  1:117332/1‑150 (MQ=255)                                       aTTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGc                                                                                                           <  2:176798/151‑1 (MQ=255)                                              aaGCGAATTTTAAGACGTTGTTCCAGGGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTg                                                                                                    <  2:1025553/151‑1 (MQ=255)                                                    aTTTTAAGACGTTGTTCCAGTGTTTGAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGCAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATc                                                                                              <  1:756508/151‑1 (MQ=255)                                                     ttttAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATTGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATcc                                                                                             <  1:798068/151‑1 (MQ=255)                                                     ttttAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATcc                                                                                             <  2:577138/151‑1 (MQ=255)                                                               ttgttCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATTGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTaa                                                                                     >  1:349487/1‑149 (MQ=255)                                                               ttgttCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTaa                                                                                     >  1:445018/1‑149 (MQ=255)                                                               ttgttCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTaa                                                                                     >  2:602679/1‑149 (MQ=255)                                                               ttgttCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAAc                                                                                    <  1:97943/150‑1 (MQ=255)                                                               ttgttCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACt                                                                                   <  2:477223/151‑1 (MQ=255)                                                               ttgttCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGAATTAGTGAAAATCCTTCATTaa                                                                                     >  2:698764/1‑149 (MQ=255)                                                                    ccAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATcc                                                                              <  1:324271/151‑1 (MQ=255)                                                                    ccAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATcc                                                                              <  2:923037/151‑1 (MQ=255)                                                                        tgtTTTAATACTATGGATAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGaa                                                                          <  2:512930/151‑1 (MQ=255)                                                                         gtTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCCCGGCAAAGAAAGGGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAat                                                                         >  2:613771/1‑151 (MQ=255)                                                                         gtTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAat                                                                         >  1:62518/1‑151 (MQ=255)                                                                         gtTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAat                                                                         >  1:825549/1‑151 (MQ=255)                                                                         gtTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAat                                                                         >  1:990150/1‑151 (MQ=255)                                                                         gtTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAat                                                                         >  1:23450/1‑151 (MQ=255)                                                                         gtTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAat                                                                         >  2:112293/1‑151 (MQ=255)                                                                         gtTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGGGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAat                                                                         >  2:602927/1‑151 (MQ=255)                                                                              aaTTCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTGCATTAACTCATCCTGAAtcatca                                                                    <  1:839081/151‑1 (MQ=255)                                                                                 aCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGt                                                                 >  1:688807/1‑151 (MQ=255)                                                                                 aCTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGt                                                                 >  1:250520/1‑151 (MQ=255)                                                                                      ggatAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACa                                                            >  1:813053/4‑151 (MQ=255)                                                                                          aaTGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGAt                                                        <  2:117350/151‑1 (MQ=255)                                                                                          aaTGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGAt                                                        <  1:879560/151‑1 (MQ=255)                                                                                               cGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTTCAGGATTATgc                                                   <  2:445185/151‑1 (MQ=255)                                                                                               cGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATgc                                                   <  2:789471/151‑1 (MQ=255)                                                                                               cGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATgc                                                   <  1:366020/151‑1 (MQ=255)                                                                                               cGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATgc                                                   <  2:772144/151‑1 (MQ=255)                                                                                                   cGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATgccgc                                                >  1:549363/1‑150 (MQ=255)                                                                                                      ggATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTg                                             >  1:1044058/1‑150 (MQ=255)                                                                                                      ggATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTg                                             >  1:961533/1‑150 (MQ=255)                                                                                                       gATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCg                                           >  2:140996/1‑151 (MQ=255)                                                                                                       gATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCg                                           >  1:136976/1‑151 (MQ=255)                                                                                                          aaGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCGTaa                                        <  1:323851/151‑1 (MQ=255)                                                                                                            gCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCGTAAAg                                      >  2:475306/1‑151 (MQ=255)                                                                                                            gCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCGTAAAg                                      >  2:366550/1‑151 (MQ=255)                                                                                                            gCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCGTAAAg                                      >  1:813786/1‑151 (MQ=255)                                                                                                              cTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCGTAAAGTg                                    <  2:499512/151‑1 (MQ=255)                                                                                                              cTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCGTAAAGTg                                    <  2:297679/151‑1 (MQ=255)                                                                                                              cTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCGTAAAGTg                                    <  1:907612/151‑1 (MQ=255)                                                                                                                 aCTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCGTAAAGTgcgc                                 <  1:175077/151‑1 (MQ=255)                                                                                                                  cTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCGTAAAGTgcg                                  >  2:331290/1‑149 (MQ=255)                                                                                                                         cccGCTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCGTAAAGTGCGCTCCAGAAc                         <  1:1018591/151‑1 (MQ=255)                                                                                                                             cTGCACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCGTAAAGTGCGCTCCAGAACTTaa                     >  2:992598/1‑151 (MQ=255)                                                                                                                                cACGAAAGCTTTGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCGTAAAGTGCGCTCCAGAACTTAACGt                  <  1:537694/151‑1 (MQ=255)                                                                                                                                          ttGATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCGTAAAGTGCGCTCCAGAACTTAACGTGGAGGTaaaa        <  1:698507/151‑1 (MQ=255)                                                                                                                                            gATTACGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCGTAAAGTGCGCTCCCGAACTTAACGTGGAGGTAAAAtt      >  1:611655/1‑151 (MQ=255)                                                                                                                                                aCGGGTCCCGGCAAAGAAAGTGGCAAAGGCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCGTAAAGTGCGCTCCAGAACTTAACGTGGAGGTAAAATTATGc  <  2:531912/151‑1 (MQ=255)                                                                                                                                                aCGGGTCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCGTAAAGTGCGCTCCAGAACTTAACGTGGAGGTAAAATTATGc  <  2:505053/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GAACCCGCTTCCGTTAACGATACGCTTCGGGTTGTCCGATTGAATAAGCGAATTTTAAGACGTTGTTCCAGTGTTTTAATACTATGGCTAATGGCGGACGAGGATAAGCCTAACTCATCGCCCGCTGCACGAAAGCTTTGATTACGGGCCACGGCAAAGAAAGTGGCAAAGTCAGACATATTCATCCGCATTAGTGAAAATCCTTCATTAACTCATCCTGAATCATCACGTTTACAGGATTATGCCGCCTGCGTAAAGTGCGCTCCAGAACTTAACGTGGAGGTAAAATTATGC  >  NC_000913/949378‑949671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑