Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,119,438	A→G	F4L (TTT→CTT)	yceO ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,119,438	0	A	G	100.0%	202.1 / NA	54	F4L (TTT→CTT)	yceO	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/26);  total (28/26)

TATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTC  >  NC_000913/1119290‑1119582                                                                                                                                                     |                                                                                                                                                 tATTTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCGGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCg                                                                                                                                                <  1:49936/151‑1 (MQ=255)           cGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTc                                                                                                                                       <  1:236080/150‑1 (MQ=255)           cGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTc                                                                                                                                       <  2:57276/150‑1 (MQ=255)               aaaCTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAg                                                                                                                                  <  2:435871/151‑1 (MQ=255)               aaaCTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAg                                                                                                                                  <  2:230973/151‑1 (MQ=255)               aaaCTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAg                                                                                                                                  <  1:429663/151‑1 (MQ=255)                       aaTAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAat                                                                                                                          <  1:236714/151‑1 (MQ=255)                         tAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAatga                                                                                                                        <  1:471700/151‑1 (MQ=255)                              cacaTAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGatt                                                                                                                   >  1:790865/1‑151 (MQ=255)                              cacaTAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGatt                                                                                                                   >  1:383083/1‑151 (MQ=255)                               acaTAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGatta                                                                                                                  >  1:850938/1‑151 (MQ=255)                                  tAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTg                                                                                                               <  1:954999/151‑1 (MQ=255)                                    gatcagatcCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGa                                                                                                             >  1:216177/1‑151 (MQ=255)                                         gatcCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCgg                                                                                                        >  2:371866/1‑151 (MQ=255)                                         gatcCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCgg                                                                                                        >  2:892132/1‑151 (MQ=255)                                         gatcCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCgg                                                                                                        >  1:125838/1‑151 (MQ=255)                                                aaTTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGcc                                                                                                 <  1:878853/151‑1 (MQ=255)                                                 aTTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGc                                                                                                  >  2:615674/1‑149 (MQ=255)                                                 aTTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGc                                                                                                  >  2:996580/1‑149 (MQ=255)                                                     ccAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAAt                                                                                            <  2:613282/151‑1 (MQ=255)                                                     ccAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAAt                                                                                            <  1:92379/151‑1 (MQ=255)                                                      caacaaTCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATg                                                                                           <  1:408740/151‑1 (MQ=255)                                                         caaTCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCg                                                                                        >  1:717315/1‑151 (MQ=255)                                                            tCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGtca                                                                                     >  2:931948/1‑151 (MQ=255)                                                                    gaAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCaaaa                                                                             >  2:1009469/1‑151 (MQ=255)                                                                         aGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCg                                                                          >  1:1042022/1‑149 (MQ=255)                                                                           aTAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCttt                                                                      >  2:724119/1‑151 (MQ=255)                                                                                gCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTg                                                                 >  1:845809/1‑151 (MQ=255)                                                                                 cATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGt                                                                <  1:315887/151‑1 (MQ=255)                                                                                   tCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCt                                                              <  1:385879/151‑1 (MQ=255)                                                                                   tCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCt                                                              <  2:835791/151‑1 (MQ=255)                                                                                   tCAGATGCTCGCGAATATTATTGAGGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCt                                                              <  2:617109/151‑1 (MQ=255)                                                                                                      attGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTa                                           >  2:1024068/1‑151 (MQ=255)                                                                                                         gatgaGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTAttt                                        >  1:953826/1‑151 (MQ=255)                                                                                                           tgaGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTcc                                      <  2:287241/151‑1 (MQ=255)                                                                                                             agaTAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTccc                                     >  2:4593/1‑150 (MQ=255)                                                                                                                  gTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTaaaa                               >  2:879119/1‑151 (MQ=255)                                                                                                                  gTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTaaaa                               >  2:88959/1‑151 (MQ=255)                                                                                                                  gTGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTAGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTaaaa                               >  1:770430/1‑151 (MQ=255)                                                                                                                   tGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAAc                              <  1:273799/151‑1 (MQ=255)                                                                                                                   tGCAACAGCCGACGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAAc                              <  1:371744/151‑1 (MQ=255)                                                                                                                              aCGCATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAAtt                   >  2:997884/1‑151 (MQ=255)                                                                                                                                 cATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTaa                >  2:494800/1‑151 (MQ=255)                                                                                                                                 cATTAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTaa                >  2:425379/1‑151 (MQ=255)                                                                                                                                    tAAATACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAAc             <  1:272279/151‑1 (MQ=255)                                                                                                                                     aaaTACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAAc             >  1:180235/1‑150 (MQ=255)                                                                                                                                     aaaTACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAAc             >  1:739211/1‑150 (MQ=255)                                                                                                                                      aaTACTCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCg           <  1:649656/151‑1 (MQ=255)                                                                                                                                           tCCTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGccc      <  2:125857/151‑1 (MQ=255)                                                                                                                                             cTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCa     >  2:819473/1‑150 (MQ=255)                                                                                                                                             cTGTAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCa     >  2:1003677/1‑150 (MQ=255)                                                                                                                                                tAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTc  <  2:464985/150‑1 (MQ=255)                                                                                                                                                tAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTc  <  2:462801/150‑1 (MQ=255)                                                                                                                                                tAAAAGCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTc  <  1:564425/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 TATGTCAGTGCGGAAAACTAAAAATAAAACACATAGATCAGATCCATAATTGCCAACAATCCCCAGAGAAAAAGATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTC  >  NC_000913/1119290‑1119582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑