Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,144,312	A→G	V19A (GTA→GCA)	rne ←	endoribonuclease; RNA‑binding protein;RNA degradosome binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,144,312	0	A	G	100.0%	188.6 / NA	52	V19A (GTA→GCA)	rne	endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/30);  total (22/30)

AGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTACAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAGTCG  >  NC_000913/1144165‑1144458                                                                                                                                                    |                                                                                                                                                   aggaAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCa                                                                                                                                                  <  2:645220/150‑1 (MQ=255)     aaCCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGc                                                                                                                                             <  2:307217/151‑1 (MQ=255)     aaCCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGc                                                                                                                                             <  2:476006/151‑1 (MQ=255)            tcGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACgcgc                                                                                                                                      <  2:879732/150‑1 (MQ=255)              gTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCaa                                                                                                                                    >  1:11022/1‑151 (MQ=255)                    cgcCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAActc                                                                                                                                 <  1:763978/148‑1 (MQ=255)                        gTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCctg                                                                                                                           >  1:556092/1‑150 (MQ=255)                          aaTCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCctgctg                                                                                                                        <  2:490803/151‑1 (MQ=255)                                         cTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTaa                                                                                                         >  2:649474/1‑151 (MQ=255)                                          ttCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCct                                                                                                                            <  2:528630/131‑1 (MQ=255)                                          ttCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCct                                                                                                                            >  1:528398/1‑131 (MQ=255)                                          ttCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTaa                                                                                                         >  1:490000/1‑150 (MQ=255)                                            ccAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACAt                                                                                                      <  1:670413/151‑1 (MQ=255)                                            ccAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACAt                                                                                                      <  2:688232/151‑1 (MQ=255)                                                aCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCtt                                                                                                  >  1:1009633/1‑151 (MQ=255)                                                     gTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCAt                                                                                             >  1:340597/1‑151 (MQ=255)                                                                gTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTAc                                                                                   <  1:441559/150‑1 (MQ=255)                                                                 tGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTc                                                                                 <  2:179108/151‑1 (MQ=255)                                                                  gATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCa                                                                                >  2:647882/1‑151 (MQ=255)                                                                  gATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCa                                                                                >  1:34266/1‑151 (MQ=255)                                                                  gATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCa                                                                                >  1:527359/1‑151 (MQ=255)                                                                  gATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCa                                                                                >  1:948027/1‑151 (MQ=255)                                                                     tttACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCatta                                                                             <  2:327080/151‑1 (MQ=255)                                                                         ccTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCattatt                                                                           >  1:828324/1‑149 (MQ=255)                                                                            ttGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTa                                                                       >  1:52869/1‑150 (MQ=255)                                                                              gTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACAt                                                                    >  1:351254/1‑151 (MQ=255)                                                                               tAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACAt                                                                    <  2:770264/150‑1 (MQ=255)                                                                               tAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACAt                                                                    <  2:814845/150‑1 (MQ=255)                                                                                          cTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGc                                                        <  2:128689/151‑1 (MQ=255)                                                                                          cTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGc                                                        <  2:225648/151‑1 (MQ=255)                                                                                          cTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGc                                                        <  2:56673/151‑1 (MQ=255)                                                                                                       tGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGTAAAGt                                            <  2:889893/150‑1 (MQ=255)                                                                                                            tGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGcc                                      <  2:97315/151‑1 (MQ=255)                                                                                                            tGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGcc                                      <  2:333353/151‑1 (MQ=255)                                                                                                            tGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGcc                                      <  2:280909/151‑1 (MQ=255)                                                                                                              gACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCt                                     <  1:694837/150‑1 (MQ=255)                                                                                                                  ttCGATATCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCg                                >  1:103368/1‑151 (MQ=255)                                                                                                                        atCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGtgtg                          >  1:986716/1‑151 (MQ=255)                                                                                                                        atCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGtgtg                          >  1:837114/1‑151 (MQ=255)                                                                                                                        atCCAGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGtgtg                          >  1:206203/1‑151 (MQ=255)                                                                                                                            aGGTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAAc                       >  1:381630/1‑150 (MQ=255)                                                                                                                             ggTCATACAGACGCTGCCCATCTGCAAGGGCCACGCGCAACTCTTCCTGCTGCGTTGCGTTGATTAACATTCTTTTCATCGTCACTTACTCATTATTCTTACATTGACGACTACGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGCACCg                     >  1:484094/1‑151 (MQ=255)                                                                                                                              gTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAAc                       <  2:718818/148‑1 (MQ=255)                                                                                                                              gTCATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAAc                       <  2:96810/148‑1 (MQ=255)                                                                                                                                cATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATg                  <  1:65694/151‑1 (MQ=255)                                                                                                                                cATACAGACGCTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATg                  <  2:22528/151‑1 (MQ=255)                                                                                                                                  tACAGACGCGGCCCATCTGCAAGGGCAACGCGCACCTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGCGTGAACCGATgg                 >  1:334181/1‑150 (MQ=255)                                                                                                                                          cTGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTAgtgt         <  2:12764/150‑1 (MQ=255)                                                                                                                                           tGCCCATCTGCAAGGGCAACGCGCATCTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCt       <  1:662971/151‑1 (MQ=255)                                                                                                                                           tGCCCATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCt       <  2:11641/151‑1 (MQ=255)                                                                                                                                              ccATCTGCAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAgt    <  2:537516/151‑1 (MQ=255)                                                                                                                                                ctCTGCAGGGGCAACGCGCCACTCTTCCTGCTGAGTTGCGTTGCTTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAgtcg  >  1:98736/2‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                   AGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGACTTTCGATATCCAGGTCATACAGACGCTGCCCATCTACAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATCGTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAGTCG  >  NC_000913/1144165‑1144458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑