Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,824,686 G→A A297A (GCC→GCT mltB ← membrane‑bound lytic murein transglycosylase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,824,6860GA98.7% 272.8 / ‑4.4 74A297A (GCC→GCTmltBmembrane‑bound lytic murein transglycosylase B
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (36/37);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

TTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCGGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAATAGTTCGCCACGCTACCGATCGCATCAACCGG  >  NC_000913/2824541‑2824832
                                                                                                                                                 |                                                                                                                                                  
ttGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGcagcgg                                                                                                                                                >  1:624804/1‑150 (MQ=255)
   cGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAg                                                                                                                                            >  1:434469/1‑151 (MQ=255)
    gTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAg                                                                                                                                            >  2:202548/1‑150 (MQ=255)
         gggTGCTGCGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACTTCCAGACGCATCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGa                                                                                                                                      >  1:823058/1‑151 (MQ=255)
           gTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGaaa                                                                                                                                    >  1:1005446/1‑151 (MQ=255)
                gtgGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATg                                                                                                                                  >  1:827965/1‑148 (MQ=255)
                gtgGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATg                                                                                                                                  >  2:766088/1‑148 (MQ=255)
                 tgGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTg                                                                                                                               <  1:118984/150‑1 (MQ=255)
                  ggtggtAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTa                                                                                                                             >  1:96087/1‑151 (MQ=255)
                  ggTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAACGGCAAGCTGCGAAATGCTGTa                                                                                                                             >  1:217960/1‑151 (MQ=255)
                  ggTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGGTGCCCAGCGGCAGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTa                                                                                                                             >  1:870451/1‑151 (MQ=255)
                   gTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTa                                                                                                                             >  2:978950/1‑150 (MQ=255)
                   gTTGTAACGGGCGATGGTGTAGAAGTTCGGCAGGCCTTACCAGTACTGGCAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGCTGCCCAGCGGCTCCTGTGGCGCTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTa                                                                                                                             >  1:584602/1‑150 (MQ=255)
                    ttGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTAc                                                                                                                            <  1:322739/150‑1 (MQ=255)
                    ttGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTAc                                                                                                                            <  1:319627/150‑1 (MQ=255)
                    ttGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTAc                                                                                                                            <  2:562416/150‑1 (MQ=255)
                        aaCGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGt                                                                                                                       <  2:229259/151‑1 (MQ=255)
                        aaCGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGt                                                                                                                       <  2:526281/151‑1 (MQ=255)
                        aaCGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGt                                                                                                                       <  2:768882/151‑1 (MQ=255)
                          cGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGttn                                                                                                                     <  2:1955/151‑2 (MQ=255)
                           gggTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGtttt                                                                                                                    >  2:984557/1‑151 (MQ=255)
                                   gtgtAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGCAATGCTGTACTTAGTTTTGAAGCCAt                                                                                                            >  1:836809/1‑151 (MQ=255)
                                   gtgtAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCAt                                                                                                            >  1:566463/1‑151 (MQ=255)
                                   gtgtAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCAt                                                                                                            >  1:380814/1‑151 (MQ=255)
                                   gtgtAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCAt                                                                                                            >  1:803426/1‑151 (MQ=255)
                                   gtgtAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCAt                                                                                                            >  2:698587/1‑151 (MQ=255)
                                   gtgtAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGGGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCAt                                                                                                            >  2:471825/1‑151 (MQ=255)
                                   gtgtAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGGTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCAt                                                                                                            >  1:369155/1‑151 (MQ=255)
                                    tgaaGAAGTTCGGCAGACCGTACAAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCAtt                                                                                                           >  1:328719/4‑151 (MQ=255)
                                         aaGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGc                                                                                                       <  1:946532/150‑1 (MQ=255)
                                                    aCCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAg                                                                                             <  1:799095/149‑1 (MQ=255)
                                                    aCCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGcc                                                                                           <  1:915642/151‑1 (MQ=255)
                                                        tACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCGGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGa                                                                                        >  2:253833/1‑150 (MQ=255)
                                                        tACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGa                                                                                        >  1:1004936/1‑150 (MQ=255)
                                                        tACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGa                                                                                        >  1:353065/1‑150 (MQ=255)
                                                        tACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGAc                                                                                       <  2:762361/151‑1 (MQ=255)
                                                        tACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGAc                                                                                       <  2:290432/151‑1 (MQ=255)
                                                        tACCAGTACGGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGAc                                                                                       <  1:112816/151‑1 (MQ=255)
                                                         tccAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGAcc                                                                                      <  2:1005804/150‑1 (MQ=255)
                                                          ccAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCg                                                                                     <  1:580344/151‑1 (MQ=255)
                                                             gTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGttt                                                                                  >  1:345480/1‑151 (MQ=255)
                                                               aCTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTg                                                                                 >  2:520930/1‑150 (MQ=255)
                                                                       ccGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACcg                                                                        <  2:961549/151‑1 (MQ=255)
                                                                       ccGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACcg                                                                        <  1:1046337/151‑1 (MQ=255)
                                                                            gCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTCGCCATTACCGCGAc                                                                    >  1:842369/1‑150 (MQ=255)
                                                                             ccAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGAcc                                                                   <  2:132040/150‑1 (MQ=255)
                                                                             ccAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGAcc                                                                   <  2:337183/150‑1 (MQ=255)
                                                                                 cATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGAt                                                               >  1:18604/1‑150 (MQ=255)
                                                                                           cagcagGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCAc                                                     >  1:977378/1‑150 (MQ=255)
                                                                                           cagcagGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCAc                                                     >  1:909135/1‑150 (MQ=255)
                                                                                           cagcagGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCAc                                                     >  1:752339/1‑150 (MQ=255)
                                                                                             gcagGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCa                                                  >  1:203995/1‑151 (MQ=255)
                                                                                              cagGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCAccc                                                   <  1:97634/149‑1 (MQ=255)
                                                                                              cagGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCAcc                                                    <  2:1038125/148‑1 (MQ=255)
                                                                                              cagGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCAcc                                                    <  2:758372/148‑1 (MQ=255)
                                                                                               cggctggctTGTTGATGGTTTCCCAGCGGCCGCTGTGGCCTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAg                                                 >  1:668274/2‑150 (MQ=255)
                                                                                                 gctggctTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGc                                                <  1:966109/149‑1 (MQ=255)
                                                                                                              tGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAAt                                 <  2:195161/151‑1 (MQ=255)
                                                                                                              tGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAAt                                 <  2:688731/151‑1 (MQ=255)
                                                                                                                 ttGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAATAg                               <  1:520702/150‑1 (MQ=255)
                                                                                                                  tGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAATAGtt                             <  1:393728/151‑1 (MQ=255)
                                                                                                                  tGCCCAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAATAGtt                             <  1:726237/151‑1 (MQ=255)
                                                                                                                    cccAGCGGCTGCTGTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAATAGTTCg                           >  1:825284/1‑151 (MQ=255)
                                                                                                                             tgctgTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAATAGTTCGCCACGCTAc                  <  1:984207/151‑1 (MQ=255)
                                                                                                                               ctgTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGGGCCTGACCGTTTGCCATTACCGCGCCCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAATAGTTCGCCACGCTACCg                <  2:550282/151‑1 (MQ=255)
                                                                                                                               ctgTGGCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAATAGTTCGCCACGCTACCg                <  1:904752/151‑1 (MQ=255)
                                                                                                                                   ggCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAATAGTTCGCCACGCTACCGATCg            >  2:791018/1‑151 (MQ=255)
                                                                                                                                    gCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAATAGTTCGCCACGCTACCGATCg            >  1:188431/1‑150 (MQ=255)
                                                                                                                                    gCGTTAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAATAGTTCGCCACGCTACCGATCg            >  2:43049/1‑150 (MQ=255)
                                                                                                                                       ttAAACCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAATAGTTCGCCACGCTACCGATCGCATc        <  2:975023/151‑1 (MQ=255)
                                                                                                                                          aaCCTGCAGCGGTAAGCGGCAAAGTGCTGTACTTAGTTTGGAAGCCATTTGGCAAGCCTGGCGCCTGTCCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAATAGTTCGCCACGCTACCGATCGCATCAAc     <  1:139985/151‑1 (MQ=255)
                                                                                                                                           aCCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAATAGTTCGCCACGCTACCGATCGCATCAAcc    <  2:553831/151‑1 (MQ=255)
                                                                                                                                           aCCTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAATAGTTCGCCACGCTACCGATCGCATCAAc     >  2:298678/1‑150 (MQ=255)
                                                                                                                                            ccTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAATAGTTCGCCACGCTACCGATCGCATCAACCg   <  2:138970/151‑1 (MQ=255)
                                                                                                                                             cTGCAGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAATAGTTCGCCACGCTACCGATCGCATCAACCgg  <  2:969863/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGCCGGTGCCAACATCCAGACGCAGCAGGCTGGCTTGTTGATGGTTGCCCAGCGGCTGCTGTGGCGTTAAACCTGCGGCGGCAAGCTGCGAAATGCTGTACTTAGTTTTGAAGCCATTTGGCAAGCCTGGAGCCTGACCGTTTGCCATTACCGCGACCTGATCGCCTTTCACCCAGCCGTGCGCTTTGAAATAGTTCGCCACGCTACCGATCGCATCAACCGG  >  NC_000913/2824541‑2824832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: