Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,346,530 C→A intergenic (+70/‑48) hpf → / → ptsN ribosome hibernation promoting factor HPF; stabilizes 100S dimers/sugar‑specific enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,346,5300CA98.6% 276.8 / ‑4.7 72intergenic (+70/‑48)hpf/ptsNribosome hibernation promoting factor HPF; stabilizes 100S dimers/sugar‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (33/38);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

TGTACGCTGCCATTGATGGCTTAATTGATAAGCTGGCACGCCAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGCTCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAA  >  NC_000913/3346381‑3346677
                                                                                                                                                     |                                                                                                                                                   
tGTACGCTGCCATTGATGGCTTAATTGATAAGCTGGCACGCCAGTTGACCAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATc                                                                                                                                                   <  2:350154/151‑1 (MQ=255)
  tACGCTGCCATTGATGGCTTAATTGATAAGCTGGCACGCCAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATc                                                                                                                                                   <  1:710407/150‑1 (MQ=255)
  tACGCTGCCATTGATGGCTTAATTGATAAGCTGGCACGCCAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATc                                                                                                                                                   <  1:1043621/150‑1 (MQ=255)
  tACGCTGCCATTGATGGCTTAATTGATAAGCTGGCACGCCAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATc                                                                                                                                                   <  2:204844/150‑1 (MQ=255)
  tACGCTGCCATTGATGGCTTAATTGATAAGCTGGCACGCCAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATc                                                                                                                                                   <  1:244925/150‑1 (MQ=255)
       tGCCATTGATGGCTTAATTGATAAGCTGGCACGCCAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGcc                                                                                                                                             <  1:374697/151‑1 (MQ=255)
        gCCATTGATGGCTTAATTGATAAGCTGGCACGCCAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGc                                                                                                                                              >  1:671548/1‑149 (MQ=255)
        gCCATTGATGGCTTAATTGATAAGCTGGCACGCCAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGc                                                                                                                                              >  2:239841/1‑149 (MQ=255)
            atGATGGCTTAATTGATAAGCTGGCACGCCAGTTGACCAAACATAAAGATAAAATAAATCAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTc                                                                                                                                        <  1:291190/150‑1 (MQ=255)
               aTGGCTTAATTGATAAGCTGGCGCGCCAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGCTCCGAGCCTGTTCCAc                                                                                                                                     <  1:785168/151‑1 (MQ=255)
               aTGGCTTAATTGATAAGCTGGCACGCCAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCAc                                                                                                                                     <  2:950171/151‑1 (MQ=255)
                       aTTGATAAGCTGGCACGCCAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAg                                                                                                                             <  2:690039/151‑1 (MQ=255)
                        ttGATAAGCTGGCACGCCAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGt                                                                                                                            >  1:173377/1‑151 (MQ=255)
                        ttGATAAGCTGGCACGCCAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGt                                                                                                                            <  2:349830/151‑1 (MQ=255)
                                   gCACGCCAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTc                                                                                                                  <  1:249315/150‑1 (MQ=255)
                                        ccAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAggg                                                                                                            >  1:155423/1‑150 (MQ=255)
                                        ccAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAgg                                                                                                             <  2:81244/150‑1 (MQ=255)
                                        ccAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAgg                                                                                                             <  2:295490/150‑1 (MQ=255)
                                        ccAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAgg                                                                                                             <  1:435533/150‑1 (MQ=255)
                                        ccAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGt                                                                                                            >  2:145649/1‑151 (MQ=255)
                                             tGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAAt                                                                                                       <  1:874925/151‑1 (MQ=255)
                                                   aaCATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGa                                                                                                  >  2:510382/1‑150 (MQ=255)
                                                   aaCATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGAc                                                                                                 <  1:884705/151‑1 (MQ=255)
                                                     cATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACaa                                                                                               >  1:175978/1‑151 (MQ=255)
                                                      aTAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACaaa                                                                                              >  2:993943/1‑151 (MQ=255)
                                                         aaGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAaataa                                                                                           >  1:812630/1‑151 (MQ=255)
                                                                aCTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACa                                                                                    >  2:739102/1‑151 (MQ=255)
                                                                  tGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAAc                                                                                  <  2:168579/151‑1 (MQ=255)
                                                                         acacTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAg                                                                           >  1:1033174/1‑151 (MQ=255)
                                                                            cTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCGATTTGTTCGGTTAATGATCCGAGCCTGTTCCACGGTTTGGGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGCTATAACTCTACAGCtt                                                                        >  2:497297/1‑151 (MQ=255)
                                                                            cTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCtt                                                                        >  2:256414/1‑151 (MQ=255)
                                                                                 tGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACACCGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGCCAAATAATGATACAACTCTACAGCTTAGCAg                                                                   <  1:564112/151‑1 (MQ=255)
                                                                                 tGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAg                                                                   <  1:827202/151‑1 (MQ=255)
                                                                                 tGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAg                                                                   <  1:509654/151‑1 (MQ=255)
                                                                                 tGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAg                                                                   <  2:851767/151‑1 (MQ=255)
                                                                                 tGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAg                                                                   <  1:969628/151‑1 (MQ=255)
                                                                                 tGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAg                                                                   <  2:242326/151‑1 (MQ=255)
                                                                                 tGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAg                                                                   <  1:30732/151‑1 (MQ=255)
                                                                                         cAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTa                                                           >  2:740850/1‑151 (MQ=255)
                                                                                         cAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTa                                                           >  1:1057089/1‑151 (MQ=255)
                                                                                           aTTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTaa                                                          >  1:844878/1‑150 (MQ=255)
                                                                                           aTTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTaa                                                          >  1:12944/1‑150 (MQ=255)
                                                                                           aTTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAAc                                                         <  2:736572/151‑1 (MQ=255)
                                                                                           aTTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAAc                                                         <  1:750674/151‑1 (MQ=255)
                                                                                           aTTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAAc                                                         <  1:674438/151‑1 (MQ=255)
                                                                                           aTTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAAc                                                         <  2:907010/151‑1 (MQ=255)
                                                                                           aTTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAAc                                                         <  1:815906/151‑1 (MQ=255)
                                                                                                  tgtgCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAAt                                                  <  2:54328/151‑1 (MQ=255)
                                                                                                   gtgCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATg                                                 >  2:311251/1‑151 (MQ=255)
                                                                                                      cATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTa                                               <  2:1052008/150‑1 (MQ=255)
                                                                                                      cATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTa                                               <  1:881196/150‑1 (MQ=255)
                                                                                                          gCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACCCGa                                          >  2:851208/1‑151 (MQ=255)
                                                                                                               cTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATg                                                 <  1:108733/139‑1 (MQ=255)
                                                                                                               cTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATg                                                 >  2:108749/1‑139 (MQ=255)
                                                                                                                 gttgtGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCcgcg                                   >  1:459692/1‑151 (MQ=255)
                                                                                                                 gttgtGCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCcgcg                                   >  1:782601/1‑151 (MQ=255)
                                                                                                                    gtgCGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTc                                 >  1:328483/1‑150 (MQ=255)
                                                                                                                       cGGCACAACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCAc                              <  1:127432/150‑1 (MQ=255)
                                                                                                                          cacaACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTc                          <  1:863546/151‑1 (MQ=255)
                                                                                                                          cacaACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTc                          <  2:148337/151‑1 (MQ=255)
                                                                                                                            caACGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCag                        >  2:218705/1‑151 (MQ=255)
                                                                                                                              aCGGGCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCagag                      <  1:917948/151‑1 (MQ=255)
                                                                                                                                 ggCCATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCaa                   >  1:445886/1‑151 (MQ=255)
                                                                                                                                    cATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCaaaaa                >  2:530503/1‑151 (MQ=255)
                                                                                                                                    cATTTGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCaaaaa                >  2:979934/1‑151 (MQ=255)
                                                                                                                                      tttGTACGGTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGCCAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCaaaaaa               >  2:755889/1‑150 (MQ=255)
                                                                                                                                              gTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGcc         >  2:514541/1‑148 (MQ=255)
                                                                                                                                              gTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGcc         >  2:402892/1‑148 (MQ=255)
                                                                                                                                              gTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGcc         >  2:1056600/1‑148 (MQ=255)
                                                                                                                                              gTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGcc         >  1:1019216/1‑148 (MQ=255)
                                                                                                                                              gTTAATGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTATACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGAGTCCACTGTCAGAGCACAAAACGCGcc         >  1:793473/1‑148 (MQ=255)
                                                                                                                                                  aTGATCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACCCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGaaa  <  2:235228/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGTACGCTGCCATTGATGGCTTAATTGATAAGCTGGCACGCCAGTTGACCAAACATAAAGATAAACTGAAACAACACTAATTGTCCGGGCAATTAGCATGTGCATGGCGGTCTGTTGTGCGGCACAACGGGCCATTTGTACGGTTAATGCTCCGAGCCTGTTCCACTGTTTGAGTGGGCAGGTTCTTAGGTGAAATTATGACAAATAATGATACAACTCTACAGCTTAGCAGTGTTCTTAACAGGGAATGTACGCGAAGCCGCGTCCACTGTCAGAGCAAAAAACGCGCCCTGGAAA  >  NC_000913/3346381‑3346677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: