Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3500CT59.7% ‑5.4 / 106.8 119S64F (TCT→TTT) bglJbgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (16/32);  new base T (71/0);  total (87/32)
Fisher's exact test for biased strand distribution p-value = 2.24e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-18

Only 100 of 126 total aligned reads displayed.

TAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT  >  NC_000913/4604200‑4604497
                                                                                                                                                       |                                                                                                                                                        
tAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCGGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                                                                                          <  1:1023196/151‑1 (MQ=255)
           gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGaa                                                                                                                                             >  2:71598/1‑151 (MQ=255)
                gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTATTTTTTTTTTTTTGCCCTGAGAAggggg                                                                                                                                        >  1:586040/1‑147 (MQ=255)
                gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCCTGGGAAggggg                                                                                                                                        >  1:484704/1‑147 (MQ=255)
                gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGaaaggag                                                                                                                                        >  2:1048736/1‑146 (MQ=255)
                gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTggg                                                                                                                                        >  1:160138/1‑149 (MQ=255)
                gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGGGAAggggg                                                                                                                                        >  1:65649/1‑147 (MQ=255)
                   tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc                                                                                                                                     <  1:279152/151‑1 (MQ=255)
                     gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATTAGGAGGGGgcggcg                                                                                                                                   >  1:257364/1‑148 (MQ=255)
                     gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGAGAAGGGGgcgccg                                                                                                                                   >  1:576031/1‑149 (MQ=255)
                     gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCag                                                                                                                                   >  1:1144676/1‑151 (MQ=255)
                     gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGAGAAGTGAGCGCag                                                                                                                                   >  2:625964/1‑151 (MQ=255)
                      taTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCag                                                                                                                                   <  2:28352/150‑1 (MQ=255)
                      taTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGCAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTCCCATTAGAAATgggggccgg                                                                                                                                  >  1:666293/1‑143 (MQ=255)
                         cGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTTCCCTTAGAAGGGAgcggcgcgag                                                                                                                               >  2:771794/1‑144 (MQ=255)
                          ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGGGCGCAGGGAgg                                                                                                                              >  1:764343/1‑151 (MQ=255)
                           gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagggaggg                                                                                                                             >  1:158889/1‑151 (MQ=255)
                            tATTGAGCGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTGCTTTTTTTTTTTTTTTCCCTTTGAAGGGAACGCGGTGAgggg                                                                                                                            >  2:636831/1‑150 (MQ=255)
                             aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATTCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGAt                                                                                                                           <  1:1091543/151‑1 (MQ=255)
                             aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGTCATGAGAAGTGAGCGCAGAGAGGGag                                                                                                                           >  2:402603/1‑150 (MQ=255)
                             aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGAt                                                                                                                           <  2:967838/151‑1 (MQ=255)
                                   agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATc                                                                                                                       <  2:1025046/149‑1 (MQ=255)
                                   agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCt                                                                                                                     <  1:939259/150‑1 (MQ=255)
                                   agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCt                                                                                                                     <  2:437836/150‑1 (MQ=255)
                                   agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCt                                                                                                                     <  1:685942/150‑1 (MQ=255)
                                       tATTCAGAAAGTTTACGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGGGTGCGCAGAGAGGGGGTATCTTGc                                                                                                                 >  2:36686/1‑149 (MQ=255)
                                        aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTCTTTTTTTGCCATGTGAAGTGTGCGCAGAGAGGGATTATCTTGc                                                                                                                 >  2:1217191/1‑148 (MQ=255)
                                         ttCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGGGAAGGGAGCGCCGAGAGGGGTTATTTTGCctga                                                                                                             >  1:379499/1‑151 (MQ=255)
                                         ttCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGAGTATCTTGCctga                                                                                                             >  2:962884/1‑151 (MQ=255)
                                                      cGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCTGCGGAGAGGGCGGAGAGAGGGATTATTTTTCCCGCCTGGACTGGGGAt                                                                                               >  1:1232699/1‑151 (MQ=255)
                                                             cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTATTTTTTTTTTTTTTGCCCTGAGAAGTGAGGGCGGAGAGGGGTTATCTTGCCTGGATGTACTGGCGATTTAAttt                                                                                        >  1:640045/1‑151 (MQ=255)
                                                             cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGGGAAGTGGGCGCCGAGAGGGATTTTTTTTCCTGGCTTAAATGGGGGTTAAGttt                                                                                        >  1:804886/1‑151 (MQ=255)
                                                             cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGGGAAGGGAGGGGAGAGAGGGATTATCTTTCCTGACTGAAATGGGGGTTAAGttt                                                                                        >  1:1133422/1‑151 (MQ=255)
                                                             cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGGGAAGTGGGCGGAGGGAGGGGGTTTTTTTCCTGACTTAACTGGCGATTAAAttt                                                                                        >  1:1036886/1‑151 (MQ=255)
                                                             cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCCTGAGGAGTGAGGGCCGAGGGGGGGTTTTTTGCCTGACTGTACTGGCGATTAAGgtt                                                                                        >  2:590922/1‑151 (MQ=255)
                                                             cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACCTAAATGGCGGTTAAAttt                                                                                        >  2:749093/1‑151 (MQ=255)
                                                             cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCATTTGCGGCGGTCACTTTTTTTTTTTTTTCCATGAGGAGTTAGCGCAGAGAGGGATTTTTTTTCCTGACCGGAATGGAGATTTAAttt                                                                                        >  2:968084/1‑151 (MQ=255)
                                                               cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCCGAGGGGGGTTATCTTGCCTGACTGAACTGGCGATTTAGtttt                                                                                       >  1:450538/1‑149 (MQ=255)
                                                               cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGGCCTTGAAAAGGGGGGCCGGGAGGGGTTTTCTTGCCTTACCGAACTGGCGCTTAAGtttt                                                                                       >  1:1118898/1‑149 (MQ=255)
                                                               cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAAGGGGAGCAGGGAGGGGTTTTCTTGCCCGACTGAACTGGCGGTTAAGgttt                                                                                       >  1:1255472/1‑149 (MQ=255)
                                                               cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCATGAGAAGGGGGGGCAGAGAGGGATTTTTTTTCCTGACTGAACCGGCGATTAAGGTTc                                                                                       >  1:733398/1‑150 (MQ=255)
                                                                 tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAAATGGCGGTTTAGTTTTcg                                                                                     >  2:524460/1‑150 (MQ=255)
                                                                 tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCCTGAGGAGGGGGCGGAGAGGGGGGTTTTTTTTCCTGTCTGAACTGGGGGTTAAGTTTTcg                                                                                     >  1:665198/1‑150 (MQ=255)
                                                                      gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTTTTTTTTTTTTTTTGCCATGAGGAGGGAGGGCAGAGAGGGCTTATCTTGCCTGACTGAAATGGCGATTTAGTTTTcgcgggt                                                                                >  1:95401/1‑147 (MQ=255)
                                                                      gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGGGAAGTGAGGGCCGAGAGGGGGTATCTTGCCTGACTGGACTGGCGGTTTAGGTTTCGCGGAc                                                                                >  2:1007400/1‑150 (MQ=255)
                                                                      gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGGGCAGAGGGGGGTTATTTTGCCTGACTGAACTGGCGATTAAGTTTTcgcggaa                                                                                >  1:509439/1‑147 (MQ=255)
                                                                       cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGGTTATTTTTTCTGTCTTAACTGGGGATTTAGGTTCCCCGTaac                                                                               >  2:65935/1‑148 (MQ=255)
                                                                         ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGGAGTGAGCGCAGCGAGGGGTTATCTTGCCTTACTTAACTGGCGATTAAGTTTCCGCGTActccg                                                                            >  2:897591/1‑147 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTTTTTTTTTTTTTTTTCCATGAGAAGGGGGGGCAGGGGGGGGTTTTTTTTCCTGACTGAAATGGGGGTTAAATTTTCGCCGAccccgggt                                                                         >  1:1170822/1‑146 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTTTTTTTTTTTTTTTGCCCTTAGAAGGGGGGGCCGAGGGGGGTTTTTTTTCCTGACTTAACTGGGGGTTAAGGTTCCGCGTACCCggggg                                                                         >  1:1196871/1‑148 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCCTGAGAAGTGGGCGCCGAGAGGGAGTATTTTGCCTTACTGAACTGGGGGTTAAAGTTTCGCGGACCCGGGGt                                                                         >  2:856381/1‑151 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGGGGGGGGTTATCTTGCCTTTCTGAACTGGGGGTTAAGTTTCCGCGGACCCGGCgg                                                                         >  1:548473/1‑150 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTTTTCCATGTGAAGGGGGCGCCGAGAGGGATTTTCTTTTCCTACTGGACTGGCGATTTAGGTTTCCCGTACCCCGCGt                                                                         >  1:781576/1‑151 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGGGCAGGGAGGGGTTTTCTTTGCTGGACTAACTGGGGGGTAAGGTTTCGCCGAACCGGCGt                                                                         >  2:474965/1‑151 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGGAGGGAGCGCAGAGGGGGGATATCTTGCCTGGCTGAACTGGGGGTTAAGTTTTCGCGGACCCCGCGt                                                                         >  1:31845/1‑151 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTCGTCATGGGAAGTGGGCGGAGGGAGGGGTTATCTTGTCTGTCTGTACCTGCGATTAAGGTTTCGCGTACCCGGCGt                                                                         >  1:531312/1‑151 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTTGGAAGGGAGCGCCGGGAGGGGTTATCTTTCCTGTCTGAACCTGCGATTAAGGTTTCGCCGACCCCGCGt                                                                         >  1:1217221/1‑151 (MQ=255)
                                                                            tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCCGACTGAACTGGCGGTTAAGGTTACGCGTACCCGGCgg                                                                         >  2:286998/1‑150 (MQ=255)
                                                                            tACCTATACCAGGCAGGAGTCATTTCAGGAAGCCATGTCGCGGATCTCGTTTGCGGCGGTTCTTTTTTTTTTTTCTGTCCTGGGAAGTGAGCGCAGGGAGGGCGTATTTTGACTGTCTGTACTTGCACTTAAATTTTCGGGTACACggggg                                                                         >  2:387853/1‑148 (MQ=255)
                                                                             aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGGGAAGTGGGGGCAGAGAGGGGTTTTCTTTCCTTACTGAACTGGCGATTAAGTTTCCGCGGACCCGGCGtt                                                                        >  1:209649/1‑151 (MQ=255)
                                                                             aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGCGAAGTGAGGGCAGAGAGGGGTTATCTTTCCTGACTGAACCGGCGATTAAGTTTTCGCGGACCCcgcggt                                                                        >  2:624769/1‑149 (MQ=255)
                                                                             aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATTTTGCCTGACTGAACTGGCGATTAAGGTTCCGCGTACCCGGCGtt                                                                        >  1:797613/1‑151 (MQ=255)
                                                                              ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCACGGGGAAGGGGGGGCGAGAGGGGTTATTTTTCCTGTCTGAACTTGCGGTTTAGGTTTCGCGGAACCgggggtt                                                                       >  1:349198/1‑146 (MQ=255)
                                                                              ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGAGCAGAGAGGGGTTATCTTGGCTGACTGGACTGGCGATTAAGTTTCCGCGGACCCGGCGgtt                                                                       >  2:1130516/1‑151 (MQ=255)
                                                                              ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTGGCGGCGGACCTTTTTTTTTTTTCTGTCCTGATTAGGGAGCGCGAAGGGGGATTTTCTCTTCCTCCTGCACTGGCGATTTATTTTCcgtttctcccgcggtt                                                                       >  2:291873/1‑137 (MQ=255)
                                                                              ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTATGCGGCGGTCTTTTTTTATTTTTTTGCCATGAGAAGGGAGCGCCGGGAGGGGTTTTCTTTCCTTACTTAACTGGCGGTTTAAGTTTCGCGCACCCGGCGttt                                                                       >  1:1142639/1‑151 (MQ=255)
                                                                                 ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGt                                                                    <  2:160107/151‑1 (MQ=255)
                                                                                   aCCAGTCAGGAGTTATTTCTAGATGCCGGGTAGGGAATCTCGTTTGCAGCGGTCATTTTTTCATTTTCTGCCAAGGGAAGTGAGCAGAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTa                                                                  <  2:923110/151‑1 (MQ=255)
                                                                                    ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCATGAGAAGGGGGGGCCGAGGGGGGTTTTTTTTCCTTACTTAACTGGCGATTTAGGTTCCGCGTACCCCGCGGTTTGTTAt                                                                 >  1:1026712/1‑151 (MQ=255)
                                                                                      aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGGGAGGGGTTATCTTTCCTGACTGAACTGGCGGTTAAGTTTTCGCGTACCCGGCGGTTTGTTATTg                                                               >  2:715326/1‑151 (MQ=255)
                                                                                      aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCCTTTTTTTTTTTTCTGCCCTGGGAAGGGAGCGGCGGGGGGGGTTTTCTTTCCTGACTGGACTTGTGGTTTAGTTTTCGCGTACCCCGGGTTTTGTTATTg                                                               >  1:235274/1‑151 (MQ=255)
                                                                                           ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCCTGAGAAGGGAGCGCAGGGGGGGGTTTTCTTTCCTGACTGAACTGGCGGTTAAGTTTCCGCGTACCCGGCGTTTTTTTATTGCgggg                                                          >  1:849200/1‑149 (MQ=255)
                                                                                                cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTTTGCCCTGAGAAAGGTGGGCGCAGGGGGATTATTTTGCCTGCATATAATAGCGGGTTAAATTCCCCGTAACCGGCGTTTTGTGATTGTGgatgattg                                                     >  2:711272/1‑149 (MQ=255)
                                                                                                cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGGGGGGTTATCTTGCCTGACTGAACTGGCGGTTTAGGTTTCGCGGACCCGGCGTTTTGTTATTTCGgaagatga                                                     >  2:179131/1‑151 (MQ=255)
                                                                                                cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGAGAAGTGAGGGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGGTTAAGTTTCCCCGGACCCCGCGGTTTGTTATTGCGgatgatgg                                                     >  2:392489/1‑150 (MQ=255)
                                                                                                cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGGGGGGGCAGGGGGGGGTTTTCTTTCCTGACTGAACTGGCGGTTAAGTTTCCGCGGACCCGGCGTTTTGTTTTTGCGgatggtga                                                     >  1:383680/1‑151 (MQ=255)
                                                                                                  tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTTCCATGGGAAGTGAGCGCAGAGAGGGGTTATTTTGCCTGACTGAACTGGCGATTAAGTTTTCCCGGACCCGGCGTTTTGTTATTGCGGGTGATGata                                                   >  1:243620/1‑151 (MQ=255)
                                                                                                        gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTg                                                <  1:900784/148‑1 (MQ=255)
                                                                                                         atccgaTGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGc                                            <  2:1030944/146‑1 (MQ=255)
                                                                                                         aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCCCTCAGAGTGAGCGCAGGGCGGGGTTATCTTTTCTGACTGAACTTGCGATTAAGTTTCCGCGTACCCCGCGTTTTGTTATTTCCGATGTTGGTAttttag                                             >  2:486758/1‑146 (MQ=255)
                                                                                                         aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGGGAGCGCAGCGAGGGGTTATCTTGCCTTACTTAACTGGCGGTTAAGTTTCCGCGTACCCCGCGGTTTGTTTTTTGGGGTGATGGTATTTAAg                                             >  1:270406/1‑150 (MQ=255)
                                                                                                         aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAg                                             <  2:548253/150‑1 (MQ=255)
                                                                                                         aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAg                                             <  2:1044263/150‑1 (MQ=255)
                                                                                                         aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGc                                            <  2:199655/151‑1 (MQ=255)
                                                                                                         aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGc                                            <  1:95824/151‑1 (MQ=255)
                                                                                                          tGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCt                                           <  2:820709/151‑1 (MQ=255)
                                                                                                                gTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCCTGAGAAAAGAACGCTGGGAGGGGGTTTTTTTCCCTACCGTAATGGCGGTTTAATTTTCGCGTAACCCGCGTTTTTTTTTTACGGATGATGCTTTTTAAACTCgg                                        >  2:372564/1‑148 (MQ=255)
                                                                                                                      gATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCATGGGAAGTGAGCGCAGAGGGGGGGTATCTTTCCTTTTTTAACTTGCGATTAAGTTTTCGCGTACCCGGCGCTTAGGTTTTTGGGATGATGAGATTGAAGATCGGGTGATTGGtt                              >  2:526383/1‑151 (MQ=255)
                                                                                                                        tctcGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTc                             <  1:154501/151‑1 (MQ=255)
                                                                                                                        tctcGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTc                             <  2:1216712/151‑1 (MQ=255)
                                                                                                                        tctcGTTTGCGGCGGTCATTTTTTCTTTTTCCGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTc                             <  2:451385/151‑1 (MQ=255)
                                                                                                                          tcGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAt                           <  2:477880/151‑1 (MQ=255)
                                                                                                                           cGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGGCGGGAGGGATTATTTTTCCTGTCTGAAATTGGGATTAAGATTTCCCCTACCCGGCGGTTAGTTATTTCGGGTGATGTTATTGTAGCTCGGCTGATTGGGTCttt                          >  2:778579/1‑151 (MQ=255)
                                                                                                                             tttGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTg                         <  2:266024/150‑1 (MQ=255)
                                                                                                                             tttGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGt                        <  2:286274/151‑1 (MQ=255)
                                                                                                                                             ttttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTgg         <  1:907517/151‑1 (MQ=255)
                                                                                                                                               ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc       <  2:804563/151‑1 (MQ=255)
                                                                                                                                               ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc       <  2:383584/151‑1 (MQ=255)
                                                                                                                                               ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc       <  2:686963/151‑1 (MQ=255)
                                                                                                                                                    tttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGgtgt  <  2:1254387/151‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                        
TAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGT  >  NC_000913/4604200‑4604497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: