Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,516,689	T→C	T382A (ACG→GCG)	yfeA ←	putative diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,516,689	0	T	C	100.0%	188.2 / NA	54	T382A (ACG→GCG)	yfeA	putative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/24);  total (30/24)

TTGTTCCAGTGAATTTGCCGACTATTCAGGATGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGTACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAACTCTTGCCCGCCTCTTGCTCCGGCGCTTGTTCCAACGCACGAAAGTTGGGTA  >  NC_000913/2516544‑2516832                                                                                                                                                  |                                                                                                                                                ttGTTCCAGTGAATTTGCCGACTATTCAGGATGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGc                                                                                                                                            <  1:948953/151‑1 (MQ=255)   gTTCCAGTGAATTTGCCGACTATTCAGGATGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCCCCCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCaa                                                                                                                                          >  2:1042577/1‑151 (MQ=255)          tGAATTTGCCGACTATTCAGGATGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTg                                                                                                                                      <  2:22860/148‑1 (MQ=255)          tGAATTTGCCGACTATTCAGGATGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTg                                                                                                                                      <  2:1152316/148‑1 (MQ=255)          tGAATTTGCCGACTATTCAGGATGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAg                                                                                                                                    <  2:620618/150‑1 (MQ=255)                     aCTATTCAGGATGTTAACCTTATGCTGGATACGCCCTTCCGTTTCCGGCCCCCGCAGCACTAACCGCAGTCCAGTACCCGGCAAGTGATACAACTTTTGGTTTTCCTGCATCAACGGCAGCAGCGCGCGTGAAATTGGACGGATACAGTg                                                                                                                         <  2:728418/150‑1 (MQ=255)                     aCTATTCAGGATGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTg                                                                                                                         <  1:920398/150‑1 (MQ=255)                     aCTATTCAGGATGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTg                                                                                                                         <  1:667399/150‑1 (MQ=255)                     aCTATTCAGGATGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTg                                                                                                                         <  1:208696/150‑1 (MQ=255)                        aTTCAGGATGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCCATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAAcg                                                                                                                     >  2:1045790/1‑151 (MQ=255)                        aTTCAGGATGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAAcg                                                                                                                     >  2:357815/1‑151 (MQ=255)                        aTTCAGGATGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAAcg                                                                                                                     >  1:550539/1‑151 (MQ=255)                        aTTCAGGATGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAAcg                                                                                                                     <  2:898147/151‑1 (MQ=255)                        aTTCAGGATGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAAcg                                                                                                                     >  2:911570/1‑151 (MQ=255)                           cAGGATGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAAcgcg                                                                                                                   >  2:403763/1‑150 (MQ=255)                             ggATGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGcat                                                                                                                >  1:808996/1‑151 (MQ=255)                               aTGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGcatca                                                                                                              >  1:171123/1‑151 (MQ=255)                               aTGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGcatca                                                                                                              >  1:556034/1‑151 (MQ=255)                                tGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTCCCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGcatcat                                                                                                             <  2:483371/151‑1 (MQ=255)                                     aCCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTCCCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGc                                                                                                        <  2:649336/151‑1 (MQ=255)                                     aCCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGc                                                                                                        <  1:1261785/151‑1 (MQ=255)                                      ccATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGc                                                                                                        >  1:975380/1‑150 (MQ=255)                                          atGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGGGCGGATACAGTGAACGCGCATCATTAAGCCGTaa                                                                                                   >  1:651164/1‑151 (MQ=255)                                          atGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTaa                                                                                                   >  2:275159/1‑151 (MQ=255)                                             cTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGGGCGGATACAGTGAACGCGCATCATTAAGCCGTAATga                                                                                                <  2:507684/151‑1 (MQ=255)                                              tGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgac                                                                                               <  2:1090666/151‑1 (MQ=255)                                                         ttCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACGACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAAc                                                                                    <  2:931687/151‑1 (MQ=255)                                                           ccGTTTCCGGCCCGCTCAGCACTAACAGCAGGTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTc                                                                                  <  2:365234/151‑1 (MQ=255)                                                              tttCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAg                                                                               >  2:50158/1‑151 (MQ=255)                                                                        gCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATg                                                                     >  1:130677/1‑151 (MQ=255)                                                                        gCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATg                                                                     >  1:1004446/1‑151 (MQ=255)                                                                        gCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATg                                                                     >  1:359057/1‑151 (MQ=255)                                                                           cAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATgcg                                                                   >  1:1083875/1‑150 (MQ=255)                                                                             gCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgcag                                                                >  2:130002/1‑151 (MQ=255)                                                                              cACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATgcgc                                                                  <  1:336942/148‑1 (MQ=255)                                                                                cTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGCTTATCAATGCgcaggca                                                             >  2:981314/1‑151 (MQ=255)                                                                                    cagcagTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCaa                                                         >  1:1016745/1‑151 (MQ=255)                                                                                        agTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCaaaaa                                                      >  2:172517/1‑150 (MQ=255)                                                                                         gTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCaaaaa                                                      >  2:346972/1‑149 (MQ=255)                                                                                          ttCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAActc                                                   <  1:239484/151‑1 (MQ=255)                                                                                          ttCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAAct                                                    <  2:917871/150‑1 (MQ=255)                                                                                          tccACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAGTGCGCAGGAAGCAAAAActc                                                   <  2:4827/149‑1 (MQ=255)                                                                                           tCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAAct                                                    >  1:171536/1‑149 (MQ=255)                                                                                              cTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAACGGAAACAGGGAACGCGCATCATTTAGCCGTAAAGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAACTCTTg                                                >  1:964389/1‑150 (MQ=255)                                                                                                aCCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAACTCTTGccc                                             <  1:1101282/151‑1 (MQ=255)                                                                                                         ttGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAACTCTTGCCCGCCTCTTg                                     >  1:948421/1‑150 (MQ=255)                                                                                                          tGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAACTCTTGCCCGCCTCTTg                                     >  1:238372/1‑149 (MQ=255)                                                                                                                 aCTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGAtt                                                                            >  1:106036/1‑103 (MQ=255)                                                                                                                 aCTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGAtt                                                                            <  2:106012/103‑1 (MQ=255)                                                                                                                 aCTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAACTCTTGCCCGCCTCTTGCTCCGgcg                             >  1:1248943/1‑150 (MQ=255)                                                                                                                      tCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAACTCTTGCCCGCCTCTTGCTCCGGCGCTTGtt                       <  2:937975/151‑1 (MQ=255)                                                                                                                        gTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAACTCTTGCCCGCCTCTTGCTCCGGCGCTTGTTc                      >  2:469287/1‑150 (MQ=255)                                                                                                                                          cagcagCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAACTCTTGCCCGCCTCTTGCTCCGGCGCTTGTTCCAACGCACGAAAGTTGGGt   >  2:721133/1‑151 (MQ=255)                                                                                                                                             cagcGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAACTCTTGCCCGCCTCTTGCTCCGGCGCTTGTTCCAACGCACGAAAGTTGGGTa  <  1:172548/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                TTGTTCCAGTGAATTTGCCGACTATTCAGGATGTTAACCATATGCTGGAGTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGTACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAACTCTTGCCCGCCTCTTGCTCCGGCGCTTGTTCCAACGCACGAAAGTTGGGTA  >  NC_000913/2516544‑2516832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑