Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,008,3510TG54.5% 29.9 / 45.7 33G25G (GGT→GGG) yedEUPF0394 family sulphur transport domain‑containing inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (4/11);  new base G (18/0);  total (22/11)
Fisher's exact test for biased strand distribution p-value = 7.05e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.15e-04

TAAATTTGCATGGTGTATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGC  >  NC_000913/2008212‑2008499
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taaatTTGCATGGTGTATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCtac                                                                                                                                           <  1:476140/151‑1 (MQ=255)
        cATGGTGTATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTAt                                                                                                                                    <  2:8110/150‑1 (MQ=255)
            gtgtATGATTCGCGCCTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTTTCTCTACTTACTATTTTgg                                                                                                                               >  1:520654/1‑151 (MQ=255)
            gtgtATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGTTTCTCTCTTCTTTCtttttttg                                                                                                                               >  1:380711/1‑149 (MQ=255)
            gtgtATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTTTCTCTTCTTACTAtttttg                                                                                                                               >  2:411498/1‑149 (MQ=255)
            gtgtATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTCTTTCTTCTTAACAtttttg                                                                                                                               >  2:274489/1‑149 (MQ=255)
                          aCTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCAcc                                                                                                                  <  1:146539/150‑1 (MQ=255)
                                gTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTTTCTCTACTTTCTATTTTGGCATTACTTGCCCCTTTTtgg                                                                                                           >  2:150227/1‑151 (MQ=255)
                                 tttCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGc                                                                                                          <  1:450945/151‑1 (MQ=255)
                                 tttCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGc                                                                                                          <  1:162093/151‑1 (MQ=255)
                                           tCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGt                                                                                                <  1:80156/151‑1 (MQ=255)
                                                gTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCCCGGGGGAAtt                                                                                           >  1:349678/1‑151 (MQ=255)
                                                     gagGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTCTCTCTACTTACTATTTTTGCATTACTGGCACCTTTTGGGGTGTCACGGGGGGAttttac                                                                                       >  1:412522/1‑147 (MQ=255)
                                                           aCATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTCTCTCTTCTTTCTCTTTTTGCCTTTCTTGCCCCCTTTGGGCTGTCCCGGGTGAATTTTCCCCTTtgg                                                                                >  2:72941/1‑151 (MQ=255)
                                                           aCATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTCTCTCTACTTACTATTTTTGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTtgg                                                                                >  1:585069/1‑151 (MQ=255)
                                                            cATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTCTCTCTACTTAATATTTTTGCCTTTCCGGGCCCCTTTGGGCTGTCCCGGGGGGATTTAACCGTTtggg                                                                               >  2:401813/1‑151 (MQ=255)
                                                                catgtcatgGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTTTCTCTACTTACTTTTTTTGCATTACTGGCACCCTTTTGGGTGTCCCGGGGGAATTTACCCGTTTgggggg                                                                            >  2:406898/1‑150 (MQ=255)
                                                                  tgtcatgGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTTTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTATCCGTTTGGGCGGCCa                                                                         >  1:9889/1‑151 (MQ=255)
                                                                               aTTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGGGAATTTACCCGTTGGGGCGGCCCGCTCCTCCAACtt                                                            >  2:83839/1‑150 (MQ=255)
                                                                               aTTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTCTCTCTACTTACTATTTTTGCATTTATTGCACCTTTTTGGCTGTCACGGGGGAATTTACCCGGTTGGGCGGCCCGCCCCTGCCAccg                                                            >  1:363182/1‑149 (MQ=255)
                                                                                        cGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTTTCTCTTCTTTCTATTTTTGCCTTACTGGCCCCTTTTTGGCGGTCACCGGTGAAATTTCCCGTTGGGGCGGCCCCCTCCTGCAACCGGTCGGCgg                                                    >  2:267409/1‑149 (MQ=255)
                                                                                            tGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTTTCACGGGGGAATTTACCCCTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATg                                               >  2:436721/1‑151 (MQ=255)
                                                                                                tGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGGATTCTCTCTACTTACTATTTTGGCATTACTGGCACCCTTTGGGCTGTCACGGGGGAATTTATCCGTTTGGGGGGCCCGCTCCTGCCACTGTTCGGCGTCCATGCTgg                                           >  2:315122/1‑150 (MQ=255)
                                                                                                  aTTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAag                                         <  1:177829/151‑1 (MQ=255)
                                                                                                  aTTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAag                                         <  1:150189/151‑1 (MQ=255)
                                                                                                     aaaTTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGt                                      <  1:15320/151‑1 (MQ=255)
                                                                                                              gCCCCCATCCCTGCGGTCATCGCGGCGGGGATTCTCTCTACTTACCATTTTGGCATTAATGGCAACCTTTGGGCTGTCACGGGGGAATTTACCCCTTGGGGCGGCCAGCTCCTGCAACTGGTCGGGGGCCTTGCTGAAGAGAGGGGGTa                               >  1:371851/1‑149 (MQ=255)
                                                                                                               cccccATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCCCCTTTTGGGCTGTCACGGGTGAATTTGCCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACt                             <  2:419317/150‑1 (MQ=255)
                                                                                                               cccccATCCCTGCGGTCATCGCGGCGGGGATTCTCTCTACTTACTATTTTTGCATTACCGGCCCCCTTTTGGGTGTCACGGGGGAATTTACCCCTTGGGGCGGCCAGCTCCTGCAACTGTTCCGCGTCCCTGCTGAAGGGGGGGGGTACtt                            >  1:80124/1‑151 (MQ=255)
                                                                                                                     tCCCTGCGGTCATCGCGGCGGGTATTCTCCCTACTTTCTATTTTGGCCTTTCTGGCACCTTTTGGGCTGGCACGGGGGAATTTACCCGTTGGGGGGGGCAGCTCCTGCAACCGTTCGGCGTCCAAGGTGGAGAGGGGGGTGACTTTACAAt                      >  1:518481/1‑151 (MQ=255)
                                                                                                                            ggTCATCGCGGCGGGGATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTTCCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTTAAATTATcc                 >  1:85401/1‑149 (MQ=255)
                                                                                                                                    cggcggGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAg        <  2:370295/150‑1 (MQ=255)
                                                                                                                                          gTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGc  >  1:352826/1‑150 (MQ=255)
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TAAATTTGCATGGTGTATGATTCGCGACTCTTGTTTCAAACCTTCTGAGTTCAGAGGCTACATTCATGTCATGGCAGCAATTCAAACACGCCTGGTTGATTAAATTCTGGGCCCCCATCCCTGCGGTCATCGCGGCGGGTATTCTCTCTACTTACTATTTTGGCATTACTGGCACCTTTTGGGCTGTCACGGGTGAATTTACCCGTTGGGGCGGCCAGCTCCTGCAACTGTTCGGCGTCCATGCTGAAGAGTGGGGTTACTTTAAAATTATCCATCTGGAAGGATCGC  >  NC_000913/2008212‑2008499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑