Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,554,0000TG47.5% 91.0 / 19.4 101Y557S (TAC→TCC) maeAmalate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme
Reads supporting (aligned to +/- strand):  ref base T (27/25);  new base G (47/0);  total (76/25)
Fisher's exact test for biased strand distribution p-value = 2.71e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

GATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCG‑CCACGCCTTGCTGCTGCGCCATTTTGCCAACC‑GCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTAC  >  NC_000913/1553854‑1554148
                                                                                                                                                    |                                                                                                                                                       
gatgatGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGAttt                                                                                                                                                     >  2:1535530/1‑150 (MQ=255)
    atgTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑TG‑CGGGATTCGGc                                                                                                                                                  >  1:1380163/1‑150 (MQ=255)
    atgTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGc                                                                                                                                                  <  2:464204/150‑1 (MQ=255)
    atgTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCgg                                                                                                                                                   >  1:1356420/1‑149 (MQ=255)
    atgTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCgg                                                                                                                                                   >  1:955774/1‑149 (MQ=255)
    atgTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAAT‑CC‑CGGGATTCgg                                                                                                                                                   >  1:1074542/1‑149 (MQ=255)
      gTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTTGGCTTg                                                                                                                                               >  1:758769/1‑151 (MQ=255)
      gTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCTTg                                                                                                                                               >  1:761588/1‑151 (MQ=255)
        tCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGcc                                                                                                                                             <  1:738199/151‑1 (MQ=255)
        tCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCTTGc                                                                                                                                              >  1:261067/1‑150 (MQ=255)
         cTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCCTggc                                                                                                                                             >  2:61935/1‑148 (MQ=255)
         cTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTTGGCTTGCCa                                                                                                                                            >  1:999305/1‑151 (MQ=255)
         cTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCTTGcc                                                                                                                                             >  1:1027548/1‑150 (MQ=255)
         cTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGAAGGCTTAGATGGAGGTACGGCGGAAGT‑CG‑CCGTATTCGGCTTGTCa                                                                                                                                            >  2:910241/1‑151 (MQ=255)
           gCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCCG‑CGGATTTCGGCTGGccga                                                                                                                                           >  2:1182254/1‑149 (MQ=255)
           gCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCCGa                                                                                                                                          >  2:795112/1‑151 (MQ=255)
           gCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCTTGCCAGa                                                                                                                                          >  1:1512802/1‑151 (MQ=255)
           gCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCGTGCCAGa                                                                                                                                          >  2:680150/1‑151 (MQ=255)
           gCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGGAGG‑CG‑CGGGATTTGGCTTGCCCGa                                                                                                                                          >  1:284429/1‑151 (MQ=255)
              tAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCTTGCCCGaaaa                                                                                                                                       >  1:569723/1‑150 (MQ=255)
                gCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTTGGCTTTCCCGAAATTa                                                                                                                                     >  1:281601/1‑151 (MQ=255)
                   ggTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGcc                                                                                                                                             <  2:1073968/140‑1 (MQ=255)
                   ggTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAAATATCg                                                                                                                                  >  2:296526/1‑151 (MQ=255)
                   ggTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTTGGCTTTccctttctctttt                                                                                                                                  >  1:1074477/1‑140 (MQ=255)
                    gTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCg                                                                                                                                  <  1:1514432/150‑1 (MQ=255)
                       aGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGGAGT‑CG‑CGGGATTCGGCTTGCCAGAAATTATCGTCaa                                                                                                                              >  1:583292/1‑151 (MQ=255)
                            aaTGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGcc                                                                                                                        <  1:837469/151‑1 (MQ=255)
                            aaTGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAAT‑CG‑CGGGATTCGGCTTGCCCGAAATTATCGTCAA‑TGGc                                                                                                                         >  1:312097/1‑150 (MQ=255)
                                 gctttATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGT‑GC‑CGACGGGATTCGGCTTGGCAGAAAATATTGGCAA‑TGGCCTGTTg                                                                                                                   >  2:436278/3‑151 (MQ=255)
                                 gaTTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGGAGT‑CG‑CGGGATTCGGCTTGGCCGAAATTTTAGTCAA‑TGGCCTGTTg                                                                                                                   >  1:1540982/1‑151 (MQ=255)
                                  aTTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGc                                                                                                                  <  2:761155/151‑1 (MQ=255)
                                      aTTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAggg                                                                                                              <  1:226843/151‑1 (MQ=255)
                                      aTTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAggg                                                                                                              <  1:1531362/151‑1 (MQ=255)
                                      aTTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGG‑CG‑CGGGATTTGGCTTGCCAGAAAATATCGTCAA‑TGGGCTGTTGCAggg                                                                                                              >  1:372784/1‑151 (MQ=255)
                                       ttCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGc                                                                                                             <  1:295490/151‑1 (MQ=255)
                                           ccACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCTTGCCCGAAATTATTGTCAA‑TGGCCTGTTGCCGGGCCTCg                                                                                                         >  2:935181/1‑151 (MQ=255)
                                           ccACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGG‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCg                                                                                                         >  1:408257/1‑151 (MQ=255)
                                           ccACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGG‑AG‑CGGCATTTGGCTTGCCAGCAAATATCGTCTA‑CGGCCTGTTGCAgggggttg                                                                                                         >  2:1041277/1‑146 (MQ=255)
                                             aCTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCGTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGGTTCggg                                                                                                       >  2:26240/1‑150 (MQ=255)
                                                aCCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTTCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCaga                                                                                                    >  2:549744/1‑151 (MQ=255)
                                                aCCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGGCTCGGCagg                                                                                                    >  2:43632/1‑150 (MQ=255)
                                                aCCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCGGTTGCAGGGATTTGGGagg                                                                                                    >  2:513405/1‑150 (MQ=255)
                                                aCCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCTTGTCAGAAATTATCGTCAA‑TGGGCTGTTGCAGGGCTTTGGCagg                                                                                                    >  1:1506315/1‑150 (MQ=255)
                                                aCCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCTTGCCAGAAATTATCGTCAA‑AGGCCCGTTGCAGGGCTTCGGCaga                                                                                                    >  2:1425516/1‑151 (MQ=255)
                                                aCCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCTTGCCAGAAAATATCGTCAA‑TGGGCTGTTTCAGGGCTTCGGCagg                                                                                                    >  2:714369/1‑150 (MQ=255)
                                                aCCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGG‑CG‑CGGTATTTGGCTTGCCAGAAATTTTCGTCAA‑TGGACCGTTTCCAGGCTTCGGccgg                                                                                                    >  2:148986/1‑148 (MQ=255)
                                                aCCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGAAGA‑CG‑CGGGATTCGGCTTTCCCGAAATTATCGTCAA‑AGGCCTGTTGCAGGGCTTTGGCaga                                                                                                    >  1:1588351/1‑151 (MQ=255)
                                                     gTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTTGGCTTGCCAGAAATTATTGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGttt                                                                                               >  2:804615/1‑151 (MQ=255)
                                                     gTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑AGGCCTGTTGCAGGGCTTCGGCAGAGGgtt                                                                                               >  1:839875/1‑151 (MQ=255)
                                                     gTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGgtt                                                                                               >  1:953844/1‑151 (MQ=255)
                                                      tATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGttt                                                                                               <  2:1370526/150‑1 (MQ=255)
                                                       aTGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGGTTCGGCAGAGGgttt                                                                                              >  1:373349/1‑150 (MQ=255)
                                                          gATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCAc                                                                                           <  1:1095088/150‑1 (MQ=255)
                                                          gATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCAc                                                                                           <  2:1454026/150‑1 (MQ=255)
                                                          gATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGC‑CG‑CGGTATTCGGCCTGCCAGAAATTATCGTCCA‑AGGGCCGTTTCAGGGCTTCGGCAGAGGtttttca                                                                                           >  2:915742/1‑147 (MQ=255)
                                                            tGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCG‑c                                                                                       <  1:354750/151‑1 (MQ=255)
                                                               gaTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTTGGCCTGGCAGGAAATATCGTCAA‑AGGGCTGGTGCAGGGCCTCGGCAGAGGGTTTTAACG‑ccc                                                                                     >  2:491398/1‑149 (MQ=255)
                                                                aTCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCCGAAAATATCGGCAA‑TGGCCTGTTGCAGGGCTTCGGCCGAGGGTTTTACCG‑CCAc                                                                                    >  2:448073/1‑150 (MQ=255)
                                                                  cTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCG‑CCACGcc                                                                                 <  1:26246/151‑1 (MQ=255)
                                                                    gAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTTCAGGGCTTCGGCAGAGGGTTTTACCG‑CCACCCCtt                                                                               >  1:1100892/1‑151 (MQ=255)
                                                                      aaaaGGGAGAGGGAAATAGCCCGGTAGCCTTCACTCCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCG‑CCACGCCTtgc                                                                             <  1:107603/151‑1 (MQ=255)
                                                                          gggAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCTTTCCCGAAATTATCGTCAA‑TGGCCGGGTGCAGGGCTTCGGGAGAGGTTTTCACCG‑CCACGCCTttctgc                                                                          >  2:1498646/1‑150 (MQ=255)
                                                                            gaggGGGAAATAGCCCGGTAGCCTTCACTCCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCG‑CCACGCCTtgctgctg                                                                        <  1:703377/150‑1 (MQ=255)
                                                                              gagGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTTGGCTTGCCAGAAATTATCGTCTA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTCACCG‑CCCCGCCCTGCTGCTgcg                                                                      >  1:254443/1‑150 (MQ=255)
                                                                              gagGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGG‑CG‑CGGTTTTCGGCTTGCCCGAAATTTTTGTCAATTGGCCTTTTG‑AGGGGTTCGGCAGGGGGTTTTCCCC‑CCCCGCCTTTCTGGTgcg                                                                      >  1:621774/1‑150 (MQ=255)
                                                                               agGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTGGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCG‑CCACGCCTTGCTGCTgcgc                                                                     <  2:754479/150‑1 (MQ=255)
                                                                                gggAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGG‑CG‑CGGGATTCGGCTTGCCCGAAATTATCGTCAA‑TGGCCTGTTGCAGGGGTTCGGCAGAGGGTTTTACCG‑CCCCGCCTTGCTGCTGCGcc                                                                    >  2:210662/1‑150 (MQ=255)
                                                                                  gAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTTGGCCTTCCAGAAATTATCGTCAA‑ATGCCTGTTGCAGGGCTTCGGCAGAGGGTTTTACCG‑CCACGGCCTGCTGCTGCGCCAtt                                                                 >  2:1185563/1‑151 (MQ=255)
                                                                                        gCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCCGAAATTTTCGTCAA‑TGGGCTGTTGCAGGGCTTTGGCAGAGGTTTTTCCCG‑CCCCCCCTTGCTGCTGCGCCCTTTTGCCa                                                           >  2:595802/1‑151 (MQ=255)
                                                                                         cccGGTACCCTCCCCTACCGGGCGCAGGCTTAGATGGAGGTACGGCGTTAGT‑CG‑CGTTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCG‑CCACGCCTTGCTGCTGCGCCATTTTGCCa                                                           <  1:500987/150‑1 (MQ=255)
                                                                                          ccGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGGTTGCCAGAAATTATTGTCAA‑TGGCCCGTTTGCGGGCTTTGGCAGGGGTTTTCACCG‑CCACGCCCTGCTGCTGCGCCCTTTTTccca                                                          >  1:1139726/1‑148 (MQ=255)
                                                                                             gTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGGAGAGGGTTTTACCG‑CCACCCCTTGCTGCTGCGCCCTTTTTCCAAccc                                                       >  1:359245/1‑149 (MQ=255)
                                                                                             gTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGA‑CG‑CGGTATTCGGCTTGCCCGAAATTATCGTTAA‑AGGCCCGTTGCAGGGGTTCCGCAGAGGTGTTCACCG‑CCACGCCTTGCTGCTCCGCCCTTTTTCCAACC‑g                                                      >  2:258179/1‑150 (MQ=255)
                                                                                                  cTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCTTGCCAGAAAATATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTCACCG‑CCACGCCTTGCTGCTGCGCCATTTTTCCAACC‑GCCAACg                                                >  2:816155/1‑151 (MQ=255)
                                                                                                    tCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCCGAAATTATTGGCAA‑AGGCCCGTTGCAGGGCTTCGGGAGAGGGTTTTACCG‑CCACGCCTTGCTGCTGCGCCATTTTGCCAACC‑CCCAACGCa                                              >  2:742202/1‑151 (MQ=255)
                                                                                                    tCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCCGAAATTATCGTCAA‑TGGCCTGTTGCAGGGGTTCGGCAGAGGGTTTCACCG‑CCACGCCTTGCTGCTGCGCCATTTTGTCAACC‑GCAAACCCa                                              >  2:804555/1‑151 (MQ=255)
                                                                                                       cTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCCGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTCACCG‑CCACGCCTTTCTGCTGCGCCATTTTTCCAACC‑GCAAACGCAAtt                                           >  2:339398/1‑151 (MQ=255)
                                                                                                       cTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCCGAAAATATCGTCCA‑TGGCCCTGTGCCGGGCCTCGGCCGAGGGTTTTACCG‑CCACCCCTTGGTGGTGGCCCCTTTTGCCCACC‑GCCAACGCaa                                             >  2:122895/1‑149 (MQ=255)
                                                                                                        tcccGGGCGAAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCG‑CCACGCCTTGCTGCTGCGCCATTTTGCCAACC‑GCAAACGCAATTg                                          <  1:1198021/149‑1 (MQ=255)
                                                                                                        tACCGGGCGCAGGTTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATGCGGCTTGCAAGAAATTATCGTCAA‑TGCCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCG‑CCACGCCTTGCTGCTGCGCCATTTTGCCAACC‑GCAAACGCAATTg                                          <  2:927698/151‑1 (MQ=255)
                                                                                                         aCCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTTCCAGAAAATATTGTCAA‑TGGGCCGGTGCAGGGCTTCGGCAGAGGGTTTTACCG‑CCACGCCTTGCTGCTGCGCCCTTTTGCCAACC‑GCCAACGCCATTg                                          >  2:368756/1‑150 (MQ=255)
                                                                                                             ggCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTTGGCTTGCCCGAAATTATCGTCCA‑AGGGCTGTTGCAGGGCCTCGGCAGAGGGTTTTACCG‑CCACGCCCTGCTGCTGCGCCCTTTTTCCAACC‑GCAAACGCAATTGCGCgg                                     >  2:532145/1‑151 (MQ=255)
                                                                                                                   ggCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCG‑CCACGCCTTGCTGCTGCGCCATTTTGCCAACC‑GCAAACGCAATTGCGCGGGAGAc                                <  2:408143/150‑1 (MQ=255)
                                                                                                                   ggCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGGTTTTACCG‑CCACGCCTTGCTGCTGCGCCATTTTTCCAACC‑GCAAACGCAATTGCGCGGggga                                 >  1:101172/1‑149 (MQ=255)
                                                                                                                   ggCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑AGGGCTGTTGCAGGGCTTCGGCAGAGGGTTTCAACG‑CCAAGCCTTGCTGCTGCGCCATTTTGCCAAAC‑GCAAACGCAATTGGGCGGggga                                 >  1:860557/1‑149 (MQ=255)
                                                                                                                   ggCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCTTGCCCGAAATTTTCCGCCA‑TGGCCTGTTGCAGGGCTTTCGCCGAGGGTTTTACCC‑CCACGCCTTGCTGCTGCGCCCTTTTTCCCACC‑GCGAACGCCAATGCGCgggggg                                 >  2:261984/1‑146 (MQ=255)
                                                                                                                   ggCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCTTGCCAGAAATTATTGTCAA‑TGGCCTGTTTCAGGGGTTCGGCAGAGGGTTTTACCG‑CCACGCCTTGCTGCTGCGCCCTTTTGCCAACC‑GCAAACGCAATTGCGCGGgaga                                 >  2:756202/1‑149 (MQ=255)
                                                                                                                   ggCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCCGAGGGTTTTACCG‑CCACGCCTTGCTGCTGCGCCATTTTTCCAACC‑GCAAAAGCAAATTCGCGGggga                                 >  1:1093884/1‑149 (MQ=255)
                                                                                                                   ggCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCCCCG‑CCACGCCTTGCTGCTGCGCCATTTTTCCAACC‑GCAAACGCCATTGCGCGGgaga                                 >  1:1598724/1‑149 (MQ=255)
                                                                                                                   ggCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTCGGCCTTCCAGAAAATATCGTCAA‑TGGCCTGTTGCAGGGCCTTGGCAGAGGTTTTCACCC‑CCACGCCCTGCTGCTGCGCCCTTTTGCCAACC‑GCCAACGCCATTGCGCGGgaga                                 >  2:405273/1‑149 (MQ=255)
                                                                                                                     cTTAGATGGAGGTACGGCGGTAAT‑CG‑CGGGATTTGGCGTTCCCAAAAATATTGTCCA‑TAGCCCCTTGGCGGGCCTCGGCAGGGGTTTTCACCGCCCCCGCCTT‑CTTCCTCGCCCTTTTTTCAACC‑GCCAACACACTTTCGCGGGAGACctt                             >  2:262238/1‑151 (MQ=255)
                                                                                                                        aGATGGAGGTACGGCGGTAGT‑TG‑CGGGATTTGGGTTTGCAGAAAATATTCTCAA‑AGGCCTGGTGGAGGGCTTTGGCCGGGGTTTTTCCCG‑CCCAGCCCTTCTGCTGCGCCATTTTTCCCACCGGCAACCCCAATT‑CGCGGGGGACTTTTTg                          >  2:1521693/1‑151 (MQ=255)
                                                                                                                        aGATGGAGGTACGGCGGTAGT‑CG‑CGGGATTTGGGGTGGCCGAAATTATCGTCAA‑AGGCCTGTTTCAAGGCTTCGGGCGAGGTTTTCACCG‑CCACGCCCTGGTGCCGCGGCCTTTTGTCAACC‑GCCAAAGCAAATGCGCCGGAGACTTTTTg                          >  2:1410310/1‑151 (MQ=255)
                                                                                                                          agggAGGTACGGCGGTAGT‑CG‑GGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTTTTGCAGGGCTTCGGCAGAGGTTTTCACCG‑CCACGCCTTGCTGCTGCGCCATTTTGCCAACC‑GCAAACGCAATTGCGCGGGAGACTTTCTg                          <  1:333889/147‑1 (MQ=255)
                                                                                                                             gAGGTACGGCGGTAGT‑CG‑CGGGATTTGGCTTGCCAGAAATTTTCGTCCA‑TGGCCTGTTGCAGGGCTTTCGCAGAGGGTTTCACCG‑CCCCGCCCTGCTGCTGCGCCCCTTTTGCAACC‑CCAAACGCCATTGCGCGGGGGGCCTTTTGAatat                     >  2:60622/1‑151 (MQ=255)
                                                                                                                               ggTACGGCGGTAGT‑CG‑CGGTATTCGGTTTGCCAGAAATTATCGTCAA‑GGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCG‑CCACGCCTTGCTGCTGCGCCATTTTGCCAACC‑GCAAACGCAATTGCGCGGGAGACTTTCTGAATATc                    <  1:1239837/150‑1 (MQ=255)
                                                                                                                                  aCGGCGGTAGT‑CG‑GGTTATTCGGCTCGCCAGAAATTATCGTCAA‑GGGCATTTGCCAGGGCTCCGGCAGGGGTTTTCTCCG‑CCACGCCTTGCTGCTGCGCCATTTTGCCAACC‑GCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTc                <  1:303102/151‑1 (MQ=255)
                                                                                                                                                   gTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCG‑CCACGCCTTGCTGCTGCGCCATTTTGCCAACC‑GCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTAc  >  1:784365/1‑150 (MQ=255)
                                                                                                                                                   gTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCG‑CCACGCCTTGCTGCTGCGCCATTTTGCCAACC‑GCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTAc  >  2:372131/1‑150 (MQ=255)
                                                                                                                                                   gTATGCGGCTTGCAAAAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGGGTTTTTCACCG‑CCACGCCTTGCTGCTGCGCCATTTTGCCAACC‑GCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTAc  <  2:284359/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
GATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT‑CG‑CGGTATTCGGCTTGCCAGAAATTATCGTCAA‑TGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCG‑CCACGCCTTGCTGCTGCGCCATTTTGCCAACC‑GCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTAC  >  NC_000913/1553854‑1554148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑