Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,386,862	0	G	A	62.8%	32.6 / 88.7	87	intergenic (+31/‑72)	yfbL/yfbM	putative M28A family peptidase/DUF1877 family protein
Reads supporting (aligned to +/- strand):  ref base G (6/26);  new base A (54/0);  total (61/26)
Fisher's exact test for biased strand distribution p-value = 1.26e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.01e-12

ACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAA  >  NC_000913/2386712‑2387005                                                                                                                                                         |                                                                                                                                                            acCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAg                                                                                                                                                             <  2:768725/151‑1 (MQ=255)    gCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCCCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCC‑GAAAAAACGa                                                                                                                                                          >  1:1222769/1‑151 (MQ=255)              gTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCC‑GAAAAAACGAC‑AAGCACatt                                                                                                                                               >  1:31015/1‑151 (MQ=255)              gTAATAAACAATACCACTTGCCCGGTGAAACCGCAGACAGATTGAATTATCAGAAAATGGCCCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGGCA‑GAAAAAAAGAC‑Ataaaaatt                                                                                                                                               >  1:1567448/1‑144 (MQ=255)               taataaACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGCCA‑GAAAAAAAGAC‑ATGCACattt                                                                                                                                              >  1:1444311/1‑151 (MQ=255)                    aaCAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACatttta‑ttt                                                                                                                                        <  2:772487/151‑1 (MQ=255)                      cAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTGCC‑GAAAAAAAGAA‑ATTACCatttta‑ttttt                                                                                                                                      >  2:1130533/1‑150 (MQ=255)                      cAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGCCC‑GAAAAAAAGAC‑AAGCACaatttt‑ttttt                                                                                                                                      >  1:853465/1‑150 (MQ=255)                           ccACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGCCC‑AAAAAAAAGAC‑AAGCACctttta‑tttttaccca                                                                                                                                 >  1:1304401/1‑145 (MQ=255)                             aCTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATc                                                                                                                               <  1:497265/151‑1 (MQ=255)                                  cccGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTTCC‑GAAAAAAAGAA‑AAGCACATTTTT‑TTTTTCCTCATCTG‑ttt                                                                                                                         >  2:1146980/1‑151 (MQ=255)                                      gTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCC‑GAAAAAAAGAC‑AAACACAATTTT‑TTTTTCCTCCTCTG‑tttttaa                                                                                                                     >  1:1182107/1‑148 (MQ=255)                                      gTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGCCC‑CAAAAAAAAACAATCCCAATTTTT‑TTTTCCCTC‑CCTG‑tttttta                                                                                                                     >  1:829819/1‑148 (MQ=255)                                      gTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGCCA‑GAAAAAATGAA‑ATGCAAATTTTT‑TTTTTCCTCATCTG‑tttttaa                                                                                                                     >  1:1147023/1‑148 (MQ=255)                                      gTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATAAAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTTGT‑GAAAAAAAGAC‑AAGCAAATTTTT‑TTTTTCCTCTTCTG‑TTTTaaa                                                                                                                     >  1:680929/1‑150 (MQ=255)                                       tGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGa                                                                                                                    <  1:1209308/151‑1 (MQ=255)                                       tGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGa                                                                                                                    <  2:478918/151‑1 (MQ=255)                                           aCCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCC‑GAAAAAACGAC‑ATGCACATTTTT‑TTTTTCCTCCCCTT‑TTTTTAAA‑T‑GCg                                                                                                              >  1:1190825/1‑151 (MQ=255)                                                 gacagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAAAGAA‑ATGCACATTTTT‑TTTTTCCTCCTCTT‑TTTTTAAA‑T‑GCG‑AAAAAt                                                                                                       >  1:771105/1‑151 (MQ=255)                                                      aTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑t                                                                                               <  2:1016554/151‑1 (MQ=255)                                                       ttGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑tt                                                                                              <  1:1567617/151‑1 (MQ=255)                                                        tGAATTATCAGCAAATTGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGCGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCCCATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTc                                                                                             <  1:168474/151‑1 (MQ=255)                                                                  gAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCC‑GAAAAAAAGAC‑AAGCACATTTTT‑TTTTTTCTCCTCTG‑TTTTTAAA‑T‑GGG‑AAAAAA‑T‑AAA‑ATT‑AAC‑ggg‑ggga                                                                                >  1:676609/1‑149 (MQ=255)                                                                     aaTGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGaaaaa                                                                             <  1:1089789/151‑1 (MQ=255)                                                                        ggCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAAAGAC‑AAGCCCATTTTA‑TTTTTCCTCATCTG‑TTTTTAAA‑T‑GCG‑GAAAAA‑G‑AAA‑ATT‑TAA‑AGA‑GGaaaaaaaa                                                                          >  1:336265/1‑151 (MQ=255)                                                                         gCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGACCA‑GGAAAAAAGAA‑AAGGACAATTTT‑TTTTTTCTTCTCTT‑TTTTTAGA‑T‑GAG‑GAACAT‑G‑AAATTTC‑AAC‑GGG‑GGaaaaaaaa                                                                          >  2:137293/1‑151 (MQ=255)                                                                           tCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATg                                                                       <  2:1181556/151‑1 (MQ=255)                                                                             aGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAAAGAA‑ATGCACAATTTT‑TTTTTTCTCATCTG‑TTTTTAGA‑A‑ACG‑AAAAAT‑G‑AAA‑ATT‑AAA‑GGA‑GGAAAAATAAATggg                                                                     >  2:499571/1‑151 (MQ=255)                                                                             aGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGCCA‑AAAAAAAAAAC‑AATTAAAATTTT‑TTTTTCCTTATCTG‑TTTTTAAA‑A‑GGG‑AAAAATAA‑AAA‑TTA‑AAA‑GAG‑GGAAAAAAAAATgg                                                                      >  1:491919/1‑151 (MQ=255)                                                                              ggTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGTCCG‑GAAAAA‑AGAA‑AAACAAAATTTA‑TTTTTCCTCCTCTG‑TTTTTAAA‑A‑GCG‑AAAAAT‑G‑AAA‑ATT‑ACA‑GGA‑GGGAAAAAAAAAgggg                                                                    >  2:173829/1‑150 (MQ=255)                                                                              ggTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCC‑GAAAAAACGAC‑ATGGACATTTTT‑TTTTTCCTTCTCTT‑TTTTTAAA‑T‑GCC‑GAACCT‑G‑AAA‑TTT‑AAC‑GGA‑GGAAAAATAAATtggg                                                                    >  2:460907/1‑150 (MQ=255)                                                                              ggTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCACGAAAAAG‑GAA‑AAGCGCATTTTT‑TTTTTCCCTATCTC‑TTTTTATA‑A‑GCGCAAAAAT‑T‑AAT‑ATC‑AAA‑GGG‑GAAAAAAAAAATggg                                                                     >  1:1101042/1‑151 (MQ=255)                                                                              ggTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATc                                                                                                                               <  2:1495515/102‑1 (MQ=255)                                                                              ggTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAACGAC‑ATGCACATTTTT‑TTTTTCCTCATCTT‑TTTTTAAA‑T‑TCG‑AAAAAA‑A‑AAA‑ATT‑AACGGGG‑GGAAAAAAAAATggg                                                                     >  1:867184/1‑151 (MQ=255)                                                                              ggTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑CAAAAAAAGAA‑CAGAACATTTTT‑TTTTTCCTCATCTT‑TTTTTTGA‑T‑GCG‑ACAAAT‑A‑AAA‑ATA‑AACAGGG‑GGAAAAAAAAATggg                                                                     >  1:1209569/1‑151 (MQ=255)                                                                              ggTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTACA‑GAAAAAAGGCC‑ATTCACAATTTT‑TTTTTCCTTTACTG‑TTTTTAGA‑A‑GTG‑GAAAAA‑G‑AAA‑TTT‑AAA‑GGG‑GGGAAAAAAAATTGGa                                                                    >  1:781684/1‑151 (MQ=255)                                                                              ggTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGCCA‑GAAAAAAAGGC‑AAGCAAATTTTT‑TTTTTCCCCCTCTT‑TTTTTAAA‑A‑GGG‑AAAAAA‑GAAAA‑TTC‑ACG‑GGG‑GGAAAAAAAAATggg                                                                     >  1:69843/1‑151 (MQ=255)                                                                               gTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAACGGA‑AAGGACATTTTT‑TTTTTCCTCATCTG‑TTTTTAGA‑A‑GCG‑AAAAAA‑G‑AAA‑TTT‑AAA‑AGG‑GGAAAAAAAAATAGGaa                                                                   >  1:1469403/1‑151 (MQ=255)                                                                                  gTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGCGCA‑GAAAAAAAGAC‑ACGTAAATTTTA‑TTTTTTCTTATTTG‑TTTTATGA‑T‑TCG‑GAAAAC‑G‑AAA‑ATC‑AAC‑AGG‑GGAAAAATAAATGGGGATGa                                                                >  2:847086/1‑151 (MQ=255)                                                                                    ggATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGCCA‑AAAAAAACGAA‑ATGCAAAATTTT‑TTTTTCCTCATCTG‑TTTTTAAA‑A‑TAG‑AAAAAT‑G‑AAA‑ATT‑AAC‑GGA‑GGGAAAAAAAAAGGGGAAGAtt                                                              >  1:1460165/1‑151 (MQ=255)                                                                                     gATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGAtt                                                              <  1:1355369/150‑1 (MQ=255)                                                                                         gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGTTTCC‑GAAAAAAGGGC‑ATGCCCATTTTT‑TTTTTCCTCATTTT‑TTTTTAGATT‑TCA‑AAAAAT‑G‑AAA‑TTA‑ACC‑GGG‑GGAAAAA‑AAAAGGGAATGATTgggtt                                                         >  1:218333/1‑148 (MQ=255)                                                                                         gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTTCC‑GGAAAAAAAAC‑AAACAAATTTTA‑TTTTCTCCCATCTG‑TTTTTATA‑T‑GAG‑GAACAT‑A‑AAA‑ATCTAAC‑GGG‑GGAAAAATAAATggaatgttttggta                                                          >  2:1023518/1‑139 (MQ=255)                                                                                         gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTTCA‑GAAAAAAAAAC‑ATGAAAAATTTA‑TTTTTCCTCATCTG‑CTTTAATA‑A‑GCG‑AAAAAA‑G‑AAA‑ATC‑AAA‑AGA‑GGAAAAAAAAATAGGGAGGATTgggtt                                                         >  2:1478027/1‑148 (MQ=255)                                                                                         gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCC‑GAAAAAACGAC‑ATGCCCATTTTT‑TTTTTCCCCATCTT‑TTTTTAAA‑A‑GCC‑AAACAA‑A‑AAA‑TTT‑AAC‑CGA‑GGAAAAAAAAAATGGGATGATTGGCTa                                                         >  1:336507/1‑151 (MQ=255)                                                                                         gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCC‑GAAAAAACGAC‑ATGCACATTTTT‑TTTTTCCTCATCTT‑TTTTTAGA‑TGGGG‑AAAAAG‑A‑AAA‑TTA‑AAC‑GGG‑GGAAAAAAAATTGGGaagatttgggt                                                          >  1:895989/1‑142 (MQ=255)                                                                                         gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCC‑GAAAAAACGAA‑AAGCACATTTTA‑TTTTTCCTCATCTG‑TTTTTAGA‑A‑AGG‑AAAAAA‑G‑AAA‑ATT‑AAA‑GGA‑GGAAAAAAAAATGGGGATGGTTTGCTa                                                         >  2:1253946/1‑151 (MQ=255)                                                                                         gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCC‑GAAAAAAAGAA‑AATGACATTTTT‑TTTTTCCCCCTCTT‑TTTTTAAA‑TGGGG‑AAAAAT‑A‑AAA‑TCA‑AAA‑GGG‑GGAAAAAAAAAGGGGAAGGTTTggtt                                                          >  1:245181/1‑149 (MQ=255)                                                                                         gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCC‑AAAAAAAAGGC‑AAGCACATTTTT‑TTTTTCCCCCTCTGTTTTTAAAA‑G‑GGG‑AAAAAT‑A‑AAA‑TTA‑AAG‑GGG‑GGAAAAAAAAAAGGGAA‑GATTGGGTa                                                         >  1:942132/1‑151 (MQ=255)                                                                                         gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCt                                                          <  2:37048/150‑1 (MQ=255)                                                                                         gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAACGAC‑AAGACAATTTTT‑TTTTTCCCCATCTT‑TTTTTAAA‑A‑GCG‑AAAAAA‑G‑AAA‑ATT‑AAC‑AGAGGGAAAAAAAAAGGGGaagttttggtt                                                          >  1:1163924/1‑142 (MQ=255)                                                                                         gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAAAGAC‑AAGCACATTTTT‑TTTTTCCCCATCTT‑TTTTTAAA‑A‑GGG‑GAAAAA‑G‑AAA‑ATT‑AAA‑GGA‑GGGAAAAAAAATGGGGATGAtttggta                                                         >  1:1494375/1‑146 (MQ=255)                                                                                         gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAAAGAC‑AAGCAAAATTTATTTTTCCCCCCCCGG‑TTTTATAA‑T‑‑CG‑GAACAT‑A‑AAA‑TTC‑AAA‑AGA‑GGGAAAAAAAATAGGGATGATTGGCtt                                                         >  1:1050563/1‑150 (MQ=255)                                                                                         gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGGCA‑GAAAAAGGGAA‑ATGCACATTTTT‑TTTTCCCCCATCTG‑TTTTTAGA‑T‑CGG‑CAAAAT‑G‑AAA‑TTT‑AAA‑AGA‑GGAAAAAAAAAAAGGAATGATTgggtt                                                         >  1:1231080/1‑148 (MQ=255)                                                                                         gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGCCC‑TGAAAAAAGAG‑AATCACATTTTA‑ATTTTTCTTCTCTT‑GTTTAATA‑T‑GAG‑AAACAT‑G‑AAA‑ATT‑AAC‑GGC‑GGGAAAATAAAATGGAATGGTTTGCt                                                          >  2:1264079/1‑150 (MQ=255)                                                                                         gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGCCC‑GGAAAAAAGGC‑ATTCACACTTTA‑ATTTTGCTTCTCCT‑TTTTTAGA‑G‑ACG‑GAAAAA‑G‑AAA‑ATC‑ATC‑GAA‑GGAGAAATTAATgggcaatgttggccg                                                         >  2:749772/1‑139 (MQ=255)                                                                                         gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGCCC‑AAAAAAAAGAC‑AAGCAAATTTTT‑TTTTTTCTCATCTG‑TTTTAAGA‑T‑GCG‑AAAAAA‑G‑AAA‑ATC‑AAC‑GGA‑GGTAAAATAAATGGGAATGATTggtgt                                                         >  2:521522/1‑148 (MQ=255)                                                                                          tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTa                                                         <  2:1469811/150‑1 (MQ=255)                                                                                           gtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAACGAC‑AAGCAGAATTTA‑TTTTTTCTCCTCTG‑TTTTTAGA‑T‑ACG‑AAAAAA‑G‑AAA‑ATT‑AAC‑CGAGGGAAAAAAAAATGGG‑ATGATTTGCTAtt                                                       >  2:1091188/1‑151 (MQ=255)                                                                                                 aCTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTATTTTGCAg                                                 <  2:212002/151‑1 (MQ=255)                                                                                                 aCTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGCCC‑GAAAAAACGAC‑AAGCACCATTTA‑TTTTTTCTCACCCG‑GTTTTTGA‑T‑GCG‑AAAAAT‑G‑AAA‑ATT‑AAC‑AGG‑GGAAAAAAAAAAGGGAAAGATTTGGTAttttttag                                                 >  2:1614456/1‑147 (MQ=255)                                                                                                    ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAACGAC‑ATGCACATTTTT‑TTTTTCCTCCTCTG‑TTTTTAGA‑T‑GGG‑AAAAAT‑G‑AAA‑ATT‑AAC‑GGA‑GGGAAAAAAAATGGGAATGATTTGCTATTTTTTAGaaa                                              >  1:1591229/1‑151 (MQ=255)                                                                                                    ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGCCC‑GAAAAAACGAC‑AAGCACAATTTT‑TTTTTTCTCCTCTT‑TTTTTAGA‑T‑GGG‑GAAAAT‑G‑AAA‑ATT‑AAA‑GGA‑GGAAAAATAAAAGGGGATGATTGGGTATTTTTCAGaaa                                              >  2:890734/1‑151 (MQ=255)                                                                                                    ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGCCC‑AAAAAAACGAC‑ACGCACATTTTT‑TTTTTTCCCCTCCT‑TTTTTAAA‑A‑GAG‑GAAAAC‑A‑AAA‑TTC‑AAA‑GGG‑GGAGAAAAAAAAGGGGAGGAATGGGGAttttttcgaaa                                              >  1:343549/1‑144 (MQ=255)                                                                                                    ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGCCA‑GAAAAAAAGAC‑ATGCACATTTTA‑TTTTTTCTCCTCTG‑TTTTTAAA‑T‑GGG‑GAAAAA‑G‑AAA‑ATT‑AAA‑GGA‑GGAAAAAAAAAAGGGAATGATTGGCTATTTTTTAGaaa                                              >  2:246389/1‑151 (MQ=255)                                                                                                      gTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCC‑GAAAAAACGAC‑ATGCACATTTTT‑TTTTTCCTCCTCTT‑TTTTTAAA‑T‑GGGAAAAAAT‑A‑AAA‑TTA‑AAG‑GGG‑GGAAAAA‑AAAAGGGGATGATTTGGTTTTTTTTAGaaaat                                            >  1:33888/1‑149 (MQ=255)                                                                                                      gTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAAAGAA‑ATGTACATTTTT‑TTTTTCCTCCTCTT‑TTTTAAAA‑T‑GCG‑AAAAAT‑G‑AAA‑TTC‑AAC‑GGG‑GGGAAAAAAAATGGGGAAGATTGGCTATTTTGTAGaaaat                                            >  1:933768/1‑149 (MQ=255)                                                                                                      gTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGCCC‑GAAAAAACGAC‑AAGGACATTTTT‑TTTTTCCTCATCTG‑TTTTTAAA‑T‑GGG‑GAACAA‑G‑AAA‑ATC‑AAA‑GGG‑GGGAAAATAAATGGGGATGATTGGGTATTTTTCAGaaaat                                            >  1:388207/1‑149 (MQ=255)                                                                                                         ataCAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGGGCC‑GAAAAAAAGAC‑AAGAACATTTTA‑TTTTTTCTCCTCTG‑TTTTTAGA‑T‑GCG‑AAAAAT‑G‑AAA‑ATT‑AAC‑GGA‑GGGAAAATAAATGGGAAAGATTGGGTATTTTTTAGAAAATGag                                         >  2:38684/1‑150 (MQ=255)                                                                                                           acaacaGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTc                                       <  1:993846/151‑1 (MQ=255)                                                                                                            caacaGTAAATAATAAAAATTTCTCGAGGC‑GGGTTCC‑GGAAAAACGAA‑CAAAACATTTTT‑TTTTTCCTCATCTG‑TTTTTAAA‑A‑TCG‑AAAAAA‑G‑AAA‑ATA‑AAA‑GGG‑GGGAAAAAAAAAGGGAAAGATTGGGTGTTTTTCAaaaaattaggca                                      >  1:437021/1‑143 (MQ=255)                                                                                                              acaGTAAATAATAAAAATTTCTCGAGGC‑GGGGGCC‑CAAAAAAAGAC‑AAACACACTTTT‑TTTTTCCCCTTCTT‑TTTTAAAA‑A‑GCG‑GAACAA‑A‑AAA‑TTC‑AAA‑CGA‑GGGAAAAAAAATAGGGAAGATTGGCTATTTTTTAGAAAATGACTCAGa                                    >  1:980053/1‑151 (MQ=255)                                                                                                              acaGCAAATAATAAAAATTTCTCGAGGC‑GGGGCCA‑GAAAAAACGGC‑AAGGACATTTTT‑TTTTTCCTCCTCTG‑TTTTTAGA‑A‑GGG‑AAAAAT‑G‑AAA‑ATT‑AAA‑GGG‑GGGAAAAAAAATGGGGAAGATTTGCTATTTTTCAGAAAATGACTCaaa                                    >  1:1593256/1‑149 (MQ=255)                                                                                                                    aataatAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGAt                              <  2:370024/151‑1 (MQ=255)                                                                                                                    aataatAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGAt                              <  1:88205/151‑1 (MQ=255)                                                                                                                         taaAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAAcc                         <  2:951104/151‑1 (MQ=255)                                                                                                                          aaaaaTTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCa                        <  1:23191/151‑1 (MQ=255)                                                                                                                              aTTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTg                    <  1:922608/151‑1 (MQ=255)                                                                                                                                        gC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTc            <  2:467245/149‑1 (MQ=255)                                                                                                                                        gC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTc            <  2:254761/149‑1 (MQ=255)                                                                                                                                        gC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTc            <  2:1583174/149‑1 (MQ=255)                                                                                                                                            ggTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTc                                       >  1:641913/1‑119 (MQ=255)                                                                                                                                            ggTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTc                                       <  2:641622/119‑1 (MQ=255)                                                                                                                                             gTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGa      >  2:1203580/1‑151 (MQ=255)                                                                                                                                             gTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGa      >  2:1043894/1‑151 (MQ=255)                                                                                                                                              tCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGa      <  2:1056194/150‑1 (MQ=255)                                                                                                                                                   gAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGaaaaa  >  1:907293/1‑150 (MQ=255)                                                                                                                                                    aaaaaGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGaaaaa  <  2:291972/149‑1 (MQ=255)                                                                                                                                                         |                                                                                                                                                            ACCGCTTTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGC‑GGGTCCA‑GAAAAAGCGAC‑ATGCACATTTTA‑TTTTCCCTCATCTG‑TTTTAAGA‑T‑GCG‑AAACAT‑G‑AAA‑TTC‑AAC‑GGA‑GGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAA  >  NC_000913/2386712‑2387005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑