Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,665,7020CA53.7% 69.0 / 187.9 124A37S (GCG→TCG) gadXacid resistance regulon transcriptional activator; autoactivator
Reads supporting (aligned to +/- strand):  ref base C (35/22);  new base A (0/66);  total (35/89)
Fisher's exact test for biased strand distribution p-value = 5.51e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-12

Only 100 of 125 total aligned reads displayed.

GTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTG  >  NC_000913/3665553‑3665842
                                                                                                                                                         |                                                                                                                                             
gTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGACTT‑TGG‑C‑TTGCATCCGCa                                                                                                                                              >  2:1208818/1‑151 (MQ=255)
gTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCa                                                                                                                                              >  1:546717/1‑151 (MQ=255)
gTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCa                                                                                                                                              >  2:357308/1‑151 (MQ=255)
  gatgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCaaa                                                                                                                                            >  1:895187/1‑151 (MQ=255)
  gatgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCaaa                                                                                                                                            >  1:107158/1‑151 (MQ=255)
      aaCGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAAcc                                                                                                                                        <  1:375671/151‑1 (MQ=255)
      aaCGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAAcc                                                                                                                                        <  2:118992/151‑1 (MQ=255)
      aaCGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAAcc                                                                                                                                        <  2:227872/151‑1 (MQ=255)
      aaCGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑GTGCATCCGCAAAAAcc                                                                                                                                        <  2:908045/151‑1 (MQ=255)
         gAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAgg                                                                                                                                     >  2:886357/1‑151 (MQ=255)
          aGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAgg                                                                                                                                     <  1:432338/150‑1 (MQ=255)
           gTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGt                                                                                                                                    >  1:195224/1‑150 (MQ=255)
            tAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑c                                                                                                                                  <  1:462200/150‑1 (MQ=255)
                 aagaaACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAAAAATAATGAAGGCGTGTCCCTTGACACAAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGc                                                                                                                             <  2:752466/150‑1 (MQ=255)
                       cTTTTTTATGTGCATGAAGATTTAATTCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGAAACGAATCCAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCCGGC‑CACCGCCATTaaa                                                                                                                      >  1:1244544/1‑151 (MQ=255)
                        ttttttATGTCCATGAAGATTTACTGCCTCCCCCTTGAGCATCGGAAAAAATAATAAAAGCGTGTCCCTTCACACGAATACAAATTTTC‑CACCCCACTTACCTCCTCAATTT‑TGC‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTaaaa                                                                                                                     <  2:74697/151‑1 (MQ=255)
                                      aaaGATTTAATCCCTCCTCCTTGAGCATCGGAAAAAATAAGAAAGGCTTGTCCCTTACCACAAATAAAAAATTTC‑CAACCCCTTTATCTACCAAATTT‑TGC‑C‑TTGCACCCGAAAAAACCAGGC‑CACCGCCATTAAAATAGCGATATTCAcc                                                                                                       <  2:932143/150‑1 (MQ=255)
                                         gATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCAtt                                                                                                    >  2:1260460/1‑151 (MQ=255)
                                          aTTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTa                                                                                                   >  2:1239487/1‑151 (MQ=255)
                                          aTTTAATGCCCCCCCCTTGAGAATGGGGAAAAAAAATAAAGGCGTGCCCCTTGACACAAAAACAAAATTTC‑CAACACCCTTATCTATTCAAATT‑TGG‑C‑TTCCATCCGAAAAAACAAGGT‑CACCGCCATTAAAATAGCGATATTCACCAt                                                                                                     <  1:548564/149‑1 (MQ=255)
                                           tttAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGACCCTTGACACGAATACAAAATTTT‑AAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTaa                                                                                                  >  1:1287675/1‑151 (MQ=255)
                                               atccctccacctGGAGCACCGGGAAAAAAAATGAAAGCGTGTCCCTTGACACAAAAAAAAAATTTC‑CAACCCACTTTTCTACAC‑AATT‑TGG‑CTTTCCCCCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCAt                                                                                              <  2:961421/148‑1 (MQ=255)
                                               acgCCCCCCCCTTGAACATCGGGAAAAAAAATAAAAGCGTGTCCCTGGACCAAAATAAAAAATTTC‑CAACCCCCTTACCTACAAGAATT‑GGG‑C‑TTGCACCCAAAAAAACCAGGT‑CCCCGCCATTAAAATAGCGATATTCACCATTAACCAt                                                                                              <  2:827571/149‑1 (MQ=255)
                                                    tcctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTga                                                                                         >  2:794713/1‑151 (MQ=255)
                                                     cctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTgag                                                                                        >  2:402111/1‑151 (MQ=255)
                                                       tcctTGTGCATTGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAACTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAACAACCAGGT‑CCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTAAGaa                                                                                      >  2:878298/1‑151 (MQ=255)
                                                       tcctTGCGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                      >  2:516532/1‑151 (MQ=255)
                                                       tcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                      >  1:684696/1‑151 (MQ=255)
                                                       tcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                      >  2:1054057/1‑151 (MQ=255)
                                                       tcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                      >  1:1007223/1‑151 (MQ=255)
                                                       tcctGGAACATGGGGAAAAAAAAGAAAAGCGTGTCCTTGAACACAAATAAAAAATTTC‑CAACACACTTATCTACTCAATTT‑GGG‑T‑TTGCACCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                      <  2:1459600/151‑1 (MQ=255)
                                                        ctttGAGCATGGGGAAAAATAAAAAAAGCGTCTCCCTTGACACGAATAAAAATTTTT‑CAACACCCTTTTCTCCTCGATTT‑TGG‑T‑TTGCACCCGAAAAAACCGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                                                                                     <  1:825382/149‑1 (MQ=255)
                                                        ccttgggacgggggAAAAATAAAAAAAGGGTGTCCCTTGACACAAATAAAAAATTTT‑AAACCCCCTTATCTACCCAATTT‑TGG‑C‑TTGCATCCGCAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                                                                                     <  1:140769/140‑1 (MQ=255)
                                                           tGAGCATCGGGAAAAAAAATAAAAGCGGGCCCCTTGACACGAATAAAAAATTTA‑AAACCCACTTATCTACTCAATTT‑TGG‑C‑TTGCACCCAAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatata                                                                                  <  1:1563801/151‑1 (MQ=255)
                                                             aacATGGGGAAAAAAAATGAAAGCGTTCCCCTTGACACAAATAAAAAATTTT‑AAACACATTTATCCACCCAAATT‑TGG‑C‑TTCCCCCCAAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatatgtn                                                                                <  1:1567422/149‑4 (MQ=255)
                                                              gCATCGGAAAAAATAATAAAAGCGTTTCCCTTGCAACAAAAAAAAAATTTT‑CAACCCACTTATCTACTCGAATT‑TGT‑T‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                <  2:1606452/150‑1 (MQ=255)
                                                               cttgggaaaaaaTAATAAAATGGTTTCCCTAGACGCGATGACAAAATTTA‑CACCCCTCTTATCTAATCGAATG‑TGT‑T‑GGTCAGCCGCAAAAGCCAGCG‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                <  2:1343116/140‑1 (MQ=255)
                                                               cATGGGGAAAAATAATAAAAGCGTTTCCCTTGACACAAATAAAAAATTTT‑CAACACACTTATCTACTCAAATT‑TGG‑C‑TTGCATCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                <  1:273118/149‑1 (MQ=255)
                                                               cATGGGGAAAAAAAATAAAAGCGTCCCCCTTGACACAAAAAAAAAATTTC‑CAACACACTTATCTCCCCAAATT‑TGG‑C‑TTGCACCCGCAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                <  1:1149104/149‑1 (MQ=255)
                                                               cATGGGGAAAAAAAAAGAAAGCGTGTCCTTTGCCACAAATACAAAATTTT‑AAACACCCTTATCTACTCGAATT‑TGG‑C‑TTGCACCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                <  1:233187/149‑1 (MQ=255)
                                                               cATCGGGAAAAATAATGAAAGCGTGTCCTTTGACACAAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGT‑T‑TTCCACCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                <  2:323241/149‑1 (MQ=255)
                                                               cATCGGGAAAAATAATGAAAGCGTGTCCCTTGACACAAATACAAAATTTT‑CAACACACTTATCTACTCAATTT‑TGG‑C‑TTGCATCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                <  1:250656/149‑1 (MQ=255)
                                                               cATCGGAAGAAATAATGAAAGCGTTCCCCTTGACACAAATAAAAAATTTT‑CAACACATTTATCTACTCAAATT‑GGG‑T‑TTGCATCCAAAAAACCCGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                <  2:1262912/149‑1 (MQ=255)
                                                               actggggAAAAATAAGAAAAGCGTCTCCTTTAACAAAAAAAAAAAATTTA‑AAACACTCTTATCTACAC‑AATT‑TGGTT‑TTCCACCCAAAAAAACCGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                <  2:1379758/145‑1 (MQ=255)
                                                                 tggggAAAAAAAATGAAAGCGTTTCCCTTGACACGAATAAAAAATTTC‑CACCACACTTATCTACTAGATTT‑GGG‑C‑TTGCACCCAAAAAAACGAGGA‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATg                                                                            <  2:601194/149‑1 (MQ=255)
                                                                  cggaaaaaaaaaTGAAGGCGTTCCCTTTGAAACAAATAAAAAATTTT‑CAACACACTTATCTACGCGATTT‑GGT‑T‑TTCCACCCAAAAAAACAAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                           <  2:654795/145‑1 (MQ=255)
                                                                  cggaaaaaaTAATGAAAGCGTTTCCTTTGACAAAAAAAAAAAATTTT‑CACCACATTTATCTACTCAATTT‑TGT‑T‑TTGCACCCAAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATg                                                                            <  2:1014474/144‑1 (MQ=255)
                                                                  cGGGAAAAATAATGAAAGCGTGTCCCTTGACAAGAATAAAAAATTTT‑CAACACACTTATCTCCTCAATTT‑TGG‑T‑TTGCATCCAAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                           <  2:1259749/151‑1 (MQ=255)
                                                                       gaAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTg                                                                       >  1:1250935/1‑150 (MQ=255)
                                                                        aaaTAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCa                                                                     >  1:331471/1‑151 (MQ=255)
                                                                             ataaaaGCGTGTCCCTTGACACAAATAAAAAATTTA‑CAACCCACTTATCTACTCAATTT‑TGG‑C‑TTGCACCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGc                                                                <  1:767023/148‑1 (MQ=255)
                                                                             atGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACg                                                                 >  1:778677/1‑150 (MQ=255)
                                                                                  aGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTa                                                           >  2:612192/1‑151 (MQ=255)
                                                                                  aGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTa                                                           >  1:712060/1‑151 (MQ=255)
                                                                                  aGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTa                                                           >  1:854191/1‑151 (MQ=255)
                                                                                      tttCCTTTGACACAAAAAAAAAATTTT‑CAACACACTTATCTACTCAATTT‑TGG‑T‑TTGCACCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                        <  2:430595/148‑1 (MQ=255)
                                                                                      tgccctttGCCACAAAAAAAAAATTTA‑AAACCCATTTATCAACTGAAATT‑TGG‑C‑TGGCCCCCAAAAAACCCAGGC‑CCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                        <  1:133744/144‑1 (MQ=255)
                                                                                      tgccccTTGACACAAATAAAAAATTTA‑CAACACACTTATCTACTCGATTT‑TGT‑C‑TTGCATCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                        <  2:1006727/147‑1 (MQ=255)
                                                                                        ccccTTGACACAAATACAAAATTTC‑CAACACACTTATCTACTCAATTT‑TGG‑C‑TTGCATCCGAAAAAACCAGGT‑AACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAt                                                     <  2:677712/150‑1 (MQ=255)
                                                                                         cccTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc                                                                  >  1:1034457/1‑137 (MQ=255)
                                                                                           tttGACACAAATAAAAAATTTC‑CAACCCCTTTACCTACTAAATTT‑TGG‑T‑TTGCATCGAAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTcc                                                  <  2:1043416/150‑1 (MQ=255)
                                                                                           cttaacagaaaaaaaaaaTTTC‑CAACACATTTATCTACTCAATTT‑TGT‑C‑TTGCACCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTcc                                                  <  1:4973/137‑1 (MQ=255)
                                                                                            ttGACACAAATAAAAAATTAT‑AAAAACACTTATTTACTCGATTT‑TGG‑T‑TTGCACCCGAAAAAACCGGGC‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTccc                                                 <  2:315090/151‑1 (MQ=255)
                                                                                                cacaattaaaaatTTTT‑CAACACCCTTATCAACAAAAATT‑TGT‑T‑TGGCTCCCGAAAAAACCGGTT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                             <  1:1256097/142‑1 (MQ=255)
                                                                                                cacaaaaaaaaatTTTT‑CAACCCCTTTTTCTACTCAATTT‑TGG‑C‑TTGCACCCAAAAAACCCAGGT‑CCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                             <  1:376750/142‑1 (MQ=255)
                                                                                                caaaaaaaaaaaaTTTT‑AAACACCCTTATCTACTAAATTT‑TGG‑C‑TTCCACCCAAAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                             <  1:1430940/142‑1 (MQ=255)
                                                                                                  aaaaaaaaaaaTTTC‑CAACACACTTATCTTCCCAATTT‑TGG‑T‑TTACATCAAAAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                           <  1:1098177/144‑1 (MQ=255)
                                                                                                  aaaaTAAAAAATTTT‑CAACCCATTTATCTACTCGATTT‑TGG‑C‑TTCCACCCGAAAAACCCAGTT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                           <  2:83907/149‑1 (MQ=255)
                                                                                                   caaaaaaaaaTTTTCCAAACCATTTATCTACCAAATTT‑TGG‑T‑TTCCACCCAAAAAAACCGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                           <  1:1070308/145‑1 (MQ=255)
                                                                                                    aaTAAAAAATTTT‑CAACCCATTTATCTACTCAATTT‑TGG‑C‑TTGCATCCGAAAAAACCAGTT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTg                                         <  2:1602874/151‑1 (MQ=255)
                                                                                                    aaTAAAAAATTTC‑CAACCCACTTATCTACTCAATTT‑TGG‑T‑TTGCACCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTg                                         <  2:224896/151‑1 (MQ=255)
                                                                                                    aaTAAAAAATTTA‑CAACACCTTTATCTACACAATTT‑TGT‑T‑TGGCACCCCCAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                           <  2:578217/149‑1 (MQ=255)
                                                                                                     aaaCAAATTTTC‑CACCCCTCTTTTCTATTC‑AATT‑TGGCC‑TTGCACCCGAAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGa                                        <  2:472460/149‑1 (MQ=255)
                                                                                                      taaaaaaTTTT‑CAACACACTTATCTCCTCAAATT‑TGG‑C‑TTGCCTCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGAt                                       <  1:637581/148‑1 (MQ=255)
                                                                                                        cAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTg                                     >  1:1309934/1‑151 (MQ=255)
                                                                                                           aaTTTT‑CAACCCCTTTATCTCCTCAATTT‑TGG‑C‑TTGCACCCGAAAAAACCAGTT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCAt                                  <  1:595528/151‑1 (MQ=255)
                                                                                                            aTTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATa                                 >  1:1144889/1‑151 (MQ=255)
                                                                                                                      ccacTTATCACCCAGATTT‑TGG‑T‑TTGCCCCCG‑AAAAACCCGGTCCGCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTa                        <  1:150525/150‑1 (MQ=255)
                                                                                                                       cacTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                       >  1:377635/1‑151 (MQ=255)
                                                                                                                         cTTATCTCCTAGAATT‑TGG‑T‑TTGCACCCGAAAAACCCAGAT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                     <  1:1198422/151‑1 (MQ=255)
                                                                                                                          ttctcaccTCAAATT‑GGT‑C‑TTGCCACCAAAAAAGCCAGGT‑CCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  1:1350593/144‑1 (MQ=255)
                                                                                                                          ttATCTACTCAATTT‑TGG‑C‑TTGCACCCAAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  1:162597/151‑1 (MQ=255)
                                                                                                                          ttATCTACT‑GAATTGGGG‑C‑TTCCACCCAAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  2:1203702/151‑1 (MQ=255)
                                                                                                                          ttATCTACACGAATT‑TGG‑C‑TTGCATCAGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  2:1477162/151‑1 (MQ=255)
                                                                                                                          ttACCTACCCGAATT‑TGC‑C‑TTCCCCCCACAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  2:867432/151‑1 (MQ=255)
                                                                                                                          taaccacctaaattt‑tGG‑C‑TTGCATCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                    <  2:178318/138‑1 (MQ=255)
                                                                                                                           taccTACTCAATTT‑TGG‑C‑TTCCATCCGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATAtt                   <  2:674446/148‑1 (MQ=255)
                                                                                                                               tACTAGAATT‑TGG‑C‑TTGCACCCGAAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt               <  2:120120/151‑1 (MQ=255)
                                                                                                                                tactcaaTT‑TGG‑CTTTGCACCCAAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt               <  2:1500337/146‑1 (MQ=255)
                                                                                                                                    aaaTT‑GGG‑T‑TTGCCCCCGCAAAACCCAGGT‑CCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACa          <  1:1476003/150‑1 (MQ=255)
                                                                                                                                     aaTT‑TGG‑C‑TTGCACCAGAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat         <  2:546502/151‑1 (MQ=255)
                                                                                                                                     aaTT‑GGG‑C‑TTCCACCCAAAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat         <  2:669555/151‑1 (MQ=255)
                                                                                                                                      aTT‑TGG‑T‑TACCATCCGAAAAAACGGGTC‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTGCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACata        <  2:350002/151‑1 (MQ=255)
                                                                                                                                        atttgggtttGCACCCAAAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat         <  1:490992/143‑1 (MQ=255)
                                                                                                                                            g‑C‑TTGCACCCAAAAAACCCGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc    <  1:833843/150‑1 (MQ=255)
                                                                                                                                            g‑C‑TTGCACCCAAAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc    <  2:335997/150‑1 (MQ=255)
                                                                                                                                            g‑C‑TTCCACCCGAAAAAGCCAGTT‑AACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc    <  2:474891/150‑1 (MQ=255)
                                                                                                                                              ctttccacccacAAAACCCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc    <  2:849952/139‑1 (MQ=255)
                                                                                                                                              c‑TTGCACCCGCAAAACCCAGTT‑CCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTg  <  1:1144139/151‑1 (MQ=255)
                                                                                                                                              c‑TTCCACCCGAAAAAACAGGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTg  <  1:398206/151‑1 (MQ=255)
                                                                                                                                                         |                                                                                                                                             
GTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTT‑CAACACACTTATCTACTCGAATT‑TGG‑C‑TTGCATCCGCAAAAACCAGGT‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTG  >  NC_000913/3665553‑3665842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑