Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,996,015 (T)8→9 pseudogene (6/261 nt) ygeN ← pseudogene, orgB family, part of T3SS PAI ETT2 remnant

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,996,0071.T95.1% 314.8 / 6.0 82pseudogene (14/261 nt)ygeNpseudogene, orgB family, part of T3SS PAI ETT2 remnant
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base T (32/46);  total (33/49)
Fisher's exact test for biased strand distribution p-value = 6.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

TTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTA‑‑TTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTTATATAGGGTAACAAC  >  NC_000913/2995858‑2996151
                                                                                                                                                       |                                                                                                                                                  
tttCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTA‑‑t                                                                                                                                                  <  2:894571/151‑1 (MQ=255)
  tcttctCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTA‑‑ttt                                                                                                                                                <  1:409236/151‑1 (MQ=255)
     tctctcTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTA‑‑tttttt                                                                                                                                            >  1:63203/1‑151 (MQ=255)
     tctctcTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTA‑‑tttttt                                                                                                                                            <  2:321733/151‑1 (MQ=255)
        ctctTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTA‑‑ttttttttt                                                                                                                                         >  2:93644/1‑150 (MQ=255)
          ctTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAACTTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTA‑‑tttttttttcg                                                                                                                                       >  2:888239/1‑148 (MQ=255)
          ctTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAATCTCATCTCTA‑‑tttttttttcg                                                                                                                                       >  1:1376091/1‑148 (MQ=255)
          ctTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTA‑‑tttttttttcg                                                                                                                                       >  2:791078/1‑148 (MQ=255)
               ccTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATaa                                                                                                                                   >  1:1203628/1‑151 (MQ=255)
               ccTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATaa                                                                                                                                   >  1:973486/1‑151 (MQ=255)
               ccTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATaa                                                                                                                                   >  2:1374787/1‑151 (MQ=255)
               ccTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTA‑‑TTTTTTTTCGCATaaa                                                                                                                                  >  1:1459237/1‑151 (MQ=255)
                   aaTACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATg                                                                                                                               <  2:880910/151‑1 (MQ=255)
                    aTACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGt                                                                                                                              >  2:423420/1‑151 (MQ=255)
                      aCTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTaa                                                                                                                            >  1:483873/1‑151 (MQ=255)
                      aCTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTaa                                                                                                                            >  1:550681/1‑151 (MQ=255)
                      aCTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTaa                                                                                                                            <  2:938868/151‑1 (MQ=255)
                         ctGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATtc                                                                                                                         <  1:933361/151‑1 (MQ=255)
                         ctGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATtc                                                                                                                         <  1:297189/151‑1 (MQ=255)
                         ctGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATtc                                                                                                                         <  1:60421/151‑1 (MQ=255)
                           gAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATtct                                                                                                                        >  1:433731/1‑150 (MQ=255)
                           gAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATtct                                                                                                                        >  2:85841/1‑150 (MQ=255)
                                  tGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTA‑‑TTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAg                                                                                                               <  2:1423058/151‑1 (MQ=255)
                                    cGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAg                                                                                                               <  2:596369/150‑1 (MQ=255)
                                    cGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAg                                                                                                               <  1:1496733/150‑1 (MQ=255)
                                    cGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAg                                                                                                               <  2:237021/150‑1 (MQ=255)
                                    cGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAg                                                                                                               <  2:1454941/150‑1 (MQ=255)
                                        ggggtgaagaggGATTTTTTCGTGGAAAGAAACATTTTCTTTCAATCCATCAGAAGGTTTTATTTTAATCACAAAACCATTTTCAGGAGATTTTATCAAGCTCTTCTCTATTTTTTTTTCGCATAAAATGTACTTCACTCTatcagcgttt                                                                                                          >  1:769180/7‑142 (MQ=255)
                                           ttCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATg                                                                                                       <  1:1312093/151‑1 (MQ=255)
                                           ttCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATg                                                                                                       <  2:548470/151‑1 (MQ=255)
                                                   tgATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGAt                                                                                                        <  1:1170882/141‑1 (MQ=255)
                                                   ggATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCTTCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGAt                                                                                                        >  2:1171429/1‑142 (MQ=255)
                                                    gATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTgg                                                                                                <  2:760771/149‑1 (MQ=255)
                                                     aTTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTaa                                                                                             <  2:92543/151‑1 (MQ=255)
                                                       tttttCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACg                                                                                           <  2:732698/151‑1 (MQ=255)
                                                            cTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAAt                                                                                                                           >  2:1211752/1‑114 (MQ=255)
                                                            cTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAAt                                                                                                                           <  1:1211177/114‑1 (MQ=255)
                                                            cTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGAt                                                                                      >  2:499134/1‑151 (MQ=255)
                                                                aaGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTa                                                                                  >  1:298213/1‑151 (MQ=255)
                                                                   agaAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTAtg                                                                                <  1:826428/150‑1 (MQ=255)
                                                                   agaAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTAtg                                                                                <  1:252998/150‑1 (MQ=255)
                                                                   agaAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTCAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTAtg                                                                                <  1:352550/150‑1 (MQ=255)
                                                                     aaaCTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTAtg                                                                                <  2:248973/149‑1 (MQ=255)
                                                                              ctttcAATCCATCAGAATTTTTTCTTTTAATCACAACACCATTTGCAGGTGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCTTAAACTGTAATACTCTCTAACAGTGATGATGCAAGTGGTAACGGCGAACGTATTCGGTCAaaaaaa                                                                    >  1:499222/1‑151 (MQ=255)
                                                                               tttcAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAAt                                                                   <  1:1106910/151‑1 (MQ=255)
                                                                                             aaaTTTTTCTTTTAATCACAACTCCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAAtaataa                                                      <  2:600263/150‑1 (MQ=255)
                                                                                               aTTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAAtaaatt                                                   <  2:1341672/151‑1 (MQ=255)
                                                                                                tttttCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAAtaaatta                                                  >  1:373465/1‑151 (MQ=255)
                                                                                                    tCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATc                                              <  2:910987/151‑1 (MQ=255)
                                                                                                            tCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTtgctg                                       >  2:556855/1‑150 (MQ=255)
                                                                                                            tCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTtgctg                                       >  2:958491/1‑150 (MQ=255)
                                                                                                                  cacCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGc                                 >  2:1047672/1‑150 (MQ=255)
                                                                                                                   acCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCa                               >  1:407727/1‑151 (MQ=255)
                                                                                                                   acCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCa                               <  1:85805/151‑1 (MQ=255)
                                                                                                                    ccATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCaa                              >  2:656225/1‑151 (MQ=255)
                                                                                                                      aTTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATg                            >  2:834434/1‑151 (MQ=255)
                                                                                                                        ttGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAGATAATAAATTAAATCGTTGCTGCATTGCCAATGgt                          <  1:1118343/151‑1 (MQ=255)
                                                                                                                        ttGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGgt                          <  2:425310/151‑1 (MQ=255)
                                                                                                                        ttGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGgt                          <  2:1494379/151‑1 (MQ=255)
                                                                                                                        ttGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGgt                          <  1:692624/151‑1 (MQ=255)
                                                                                                                        ttGCAGGAGATTTTATCAAACTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGGTGATGCAAGTGGTAACGGCGATGGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATgat                          <  1:340821/151‑3 (MQ=255)
                                                                                                                          gCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGgttg                        >  2:1231585/1‑151 (MQ=255)
                                                                                                                           cAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGGAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGgttgt                       >  1:407242/1‑151 (MQ=255)
                                                                                                                               agaTTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGc                    >  1:769995/1‑150 (MQ=255)
                                                                                                                               agaTTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGc                    >  1:1366998/1‑150 (MQ=255)
                                                                                                                                gaTTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGc                    >  1:31071/1‑149 (MQ=255)
                                                                                                                                 aTTTTATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCtt                 >  2:349847/1‑151 (MQ=255)
                                                                                                                                 aTTGTATCAAGCTTCTCTCTATTTTTTTTTCGCATAAAATGTAATTCTCACTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGACAACAAAATCAGGAAATCATAAATTTAATCGTTGCTACATTGCCAATGGTTGTTGcc                   <  1:650779/149‑1 (MQ=255)
                                                                                                                                    ttatcaccatcctctcTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGGGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGATGTTGCCTtata              >  1:831484/13‑151 (MQ=255)
                                                                                                                                     tATCAAGCTCTTCTTTTT‑‑TTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTtatat             <  2:63236/151‑1 (MQ=255)
                                                                                                                                     tATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTtatat             <  2:818309/151‑1 (MQ=255)
                                                                                                                                     tATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTtatat             <  1:1089645/151‑1 (MQ=255)
                                                                                                                                     tATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTtatat             <  1:582127/151‑1 (MQ=255)
                                                                                                                                     tATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTtatat             <  1:596783/151‑1 (MQ=255)
                                                                                                                                     tATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTtatat             <  2:1280700/151‑1 (MQ=255)
                                                                                                                                     tATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTtatat             <  2:137258/151‑1 (MQ=255)
                                                                                                                                     tATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTtatat             <  2:2027/151‑1 (MQ=255)
                                                                                                                                     tATCAAGCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGGCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTtatat             <  1:1198727/151‑1 (MQ=255)
                                                                                                                                           gCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCa                               >  2:319128/1‑127 (MQ=255)
                                                                                                                                           gCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTTATATAGGGTa       >  2:226081/1‑151 (MQ=255)
                                                                                                                                           gCTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATNGCCa                               <  1:319040/127‑1 (MQ=255)
                                                                                                                                            cTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTTATATAGGGt        <  2:1275874/149‑1 (MQ=255)
                                                                                                                                            cTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTTATATAGGGt        <  1:878990/149‑1 (MQ=255)
                                                                                                                                            cTCATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTTATATAGGGt        <  1:57619/149‑1 (MQ=255)
                                                                                                                                              cATCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTTATATAGGGTaaaa    >  1:1298021/1‑149 (MQ=255)
                                                                                                                                               aTCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTTATATAGGGTaacaa   <  2:202690/151‑1 (MQ=255)
                                                                                                                                               aTCTCTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTTATATAGGGTaacaa   <  2:951670/151‑1 (MQ=255)
                                                                                                                                               aTCTCGA‑‑TTTTGTGTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATGAAATCGTTGCTGCATTGCCAATGGTTGTTGCCGTATATAGGGTaacaa   <  1:1078831/151‑1 (MQ=255)
                                                                                                                                                tctcTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTTATATAGGGTaacaac  <  2:1006281/151‑1 (MQ=255)
                                                                                                                                                tctcTATTTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTTATATAGGGTaacaa   >  2:821638/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                  
TTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTA‑‑TTTTTTTTCGCATAAAATGTAATTCTCTCTAAAAGTGTTGATGCAAGTGGTAACGGCGATTGTATGTGGTCAACAAAATCAGGAAATAATAAATTAAATCGTTGCTGCATTGCCAATGGTTGTTGCCTTATATAGGGTAACAAC  >  NC_000913/2995858‑2996151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: