Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,103,510	G→A	intergenic (‑314/‑441)	csgD ← / → csgB	csgBAC operon transcriptional regulator/curlin nucleator protein, minor subunit in curli complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,103,510	0	G	A	100.0%	203.8 / NA	52	intergenic (‑314/‑441)	csgD/csgB	csgBAC operon transcriptional regulator/curlin nucleator protein, minor subunit in curli complex
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/21);  total (31/21)

AATCTTAAATCAAGTGTTAAACATGTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTGATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCG  >  NC_000913/1103365‑1103656                                                                                                                                                  |                                                                                                                                                   aaTCTTAAATCAAGTGTTAAACATGTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAAc                                                                                                                                               <  1:1348227/151‑1 (MQ=255) aaTCTTAAATCAAGTGTTAAACATGTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAAc                                                                                                                                               <  2:720403/151‑1 (MQ=255) aaTCTTAAATCAAGTGTTAAACATGTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAAc                                                                                                                                               <  1:389104/151‑1 (MQ=255)    cTTAAATCAAGTGTTAAACATGTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTa                                                                                                                                            >  2:1105662/1‑151 (MQ=255)        aaTCAAGTGTTAAACATGTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACtaa                                                                                                                                        >  2:393925/1‑151 (MQ=255)         aTCAAGTGTTAAACATGTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACtaat                                                                                                                                       <  1:412398/151‑1 (MQ=255)         aTCAAGTGTTAAACATGTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACtaat                                                                                                                                       <  2:809939/151‑1 (MQ=255)           cAAGTGTTAAACATGTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACtaataa                                                                                                                                     <  1:470483/151‑1 (MQ=255)              gtgtTAAACATGTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATg                                                                                                                                   >  2:952118/1‑150 (MQ=255)              gtgcTAAACATGTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGACATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATg                                                                                                                                   >  1:297805/1‑150 (MQ=255)               tgtTAAACATGTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATg                                                                                                                                   <  1:1011920/149‑1 (MQ=255)               tgtTAAACATGTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATg                                                                                                                                   <  2:420505/149‑1 (MQ=255)               tgtTAAACATGTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCa                                                                                                                                 >  2:1437325/1‑151 (MQ=255)                gtTAAACATGTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCat                                                                                                                                >  1:1328745/1‑151 (MQ=255)                      cATGTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATaaaa                                                                                                                          >  2:485923/1‑151 (MQ=255)                      cATGTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATaaaa                                                                                                                          >  2:789842/1‑151 (MQ=255)                         gTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGc                                                                                                                                  <  1:230979/140‑1 (MQ=255)                         gTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGc                                                                                                                                  >  2:231046/1‑140 (MQ=255)                           aaCTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAatat                                                                                                                     >  2:904041/1‑151 (MQ=255)                           aaCTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAatat                                                                                                                     >  2:764223/1‑151 (MQ=255)                            aCTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATAtt                                                                                                                    >  1:1089184/1‑151 (MQ=255)                                    tAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTa                                                                                                            >  2:134946/1‑151 (MQ=255)                                        tCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAGAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTcc                                                                                                        <  2:383689/151‑1 (MQ=255)                                        tCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTcc                                                                                                        <  1:1399519/151‑1 (MQ=255)                                           ttATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCtt                                                                                                      <  2:665078/150‑1 (MQ=255)                                             atatTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCg                                                                                                   <  1:414535/151‑1 (MQ=255)                                                ttAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCa                                                                                                >  2:680722/1‑151 (MQ=255)                                                  aaaaTGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATg                                                                                              <  2:1015063/151‑1 (MQ=255)                                                     aTGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTaa                                                                                           >  2:434167/1‑151 (MQ=255)                                                           aCCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGtt                                                                                     >  1:381307/1‑151 (MQ=255)                                                            ccTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGtt                                                                                     >  2:918912/1‑150 (MQ=255)                                                            ccTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGtt                                                                                     >  1:656703/1‑150 (MQ=255)                                                                             gTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCcaca                                                                    >  1:198045/1‑150 (MQ=255)                                                                             gTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCcaca                                                                    >  1:1250550/1‑150 (MQ=255)                                                                             gTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCcaca                                                                    >  1:1507348/1‑150 (MQ=255)                                                                             gTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCACGTAAAACg                                                                                       >  2:1311625/1‑131 (MQ=255)                                                                             gTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCACGTAAAACg                                                                                       <  1:1310974/131‑1 (MQ=255)                                                                              tttAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACAcc                                                                  <  2:465008/151‑1 (MQ=255)                                                                                       gATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGa                                                          >  1:1382665/1‑150 (MQ=255)                                                                                        aTAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACa                                                        >  2:17481/1‑151 (MQ=255)                                                                                                 ttGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTAc                                               <  2:1258529/151‑1 (MQ=255)                                                                                                   gTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTa                                                >  1:163156/1‑148 (MQ=255)                                                                                                      cATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCaa                                          >  1:1387540/1‑151 (MQ=255)                                                                                                                   ttGCACAATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGttt                             >  2:783533/1‑151 (MQ=255)                                                                                                                       acaATTTTAAAAAATCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCa                         <  2:1416573/151‑1 (MQ=255)                                                                                                                                  aaaTCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTgcg              >  2:960454/1‑151 (MQ=255)                                                                                                                                   aaTCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTgcgc             <  1:731686/151‑1 (MQ=255)                                                                                                                                    aTCATACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCa            >  1:318215/1‑151 (MQ=255)                                                                                                                                        tACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGAc        <  2:155781/151‑1 (MQ=255)                                                                                                                                        tACAAATGGTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGGCCTTTCGTCATGTAAAACGTTCTTGGTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGAc        <  2:648251/151‑1 (MQ=255)                                                                                                                                               ggTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCg  >  1:452100/1‑150 (MQ=255)                                                                                                                                               ggTAATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACATCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATTTCg  >  1:1460269/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                   AATCTTAAATCAAGTGTTAAACATGTAACTAAATGTAACTCGTTATATTAAAATGTTAACCTTAAGGTTTTATTAAGTTTAGAAATGATAGAAAAGTTGTACATTTGGTTTTTATTGCACAATTTTAAAAAATCATACAAATGGTGATAACTTACTAATAATGCATATAAAAAATATTTCGGTGTAGTCCTTTCGTCATGTAAAACGTTCTTGTTTTTTCTCCACACCTCCGTGGACAATTTTTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCG  >  NC_000913/1103365‑1103656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑