Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,197,739	A→G	S167P (TCC→CCC)	ymfE ←	e14 prophage; putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,197,739	0	A	G	100.0%	294.4 / NA	81	S167P (TCC→CCC)	ymfE	e14 prophage; putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/44);  total (37/44)

ACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTT  >  NC_000913/1197596‑1197879                                                                                                                                                 |                                                                                                                                             acttTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTAGTAGGAAGGGAATTAAt                                                                                                                                       <  2:51975/151‑1 (MQ=255)   tttCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGc                                                                                                                                     <  2:433719/151‑1 (MQ=255)    ttCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCa                                                                                                                                    >  1:1134716/1‑151 (MQ=255)             gggCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGaa                                                                                                                           >  2:1283969/1‑151 (MQ=255)             gggCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGaa                                                                                                                           >  1:1200985/1‑151 (MQ=255)                     cTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaa                                                                                                                   >  1:1430882/1‑151 (MQ=255)                       gTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaaaa                                                                                                                 >  1:499112/1‑151 (MQ=255)                       gTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCaaaaa                                                                                                                 >  2:162695/1‑151 (MQ=255)                           aagcaaagATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAatat                                                                                                             >  2:847011/1‑151 (MQ=255)                           aagcaaagATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAatat                                                                                                             >  1:537827/1‑151 (MQ=255)                                 agATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACGGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaa                                                                                                       >  1:664690/1‑151 (MQ=255)                                 agATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaa                                                                                                       >  1:1145100/1‑151 (MQ=255)                                 agATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaa                                                                                                       >  2:40065/1‑151 (MQ=255)                                 agATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaa                                                                                                       >  2:546725/1‑151 (MQ=255)                                  gttaataCCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaaa                                                                                                      >  1:156482/3‑151 (MQ=255)                                  gATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaaa                                                                                                      >  2:1420942/1‑151 (MQ=255)                                  gATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaaa                                                                                                      >  2:813761/1‑151 (MQ=255)                                  gATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaaa                                                                                                      >  2:115893/1‑151 (MQ=255)                                  gATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaaa                                                                                                      >  2:1014365/1‑151 (MQ=255)                                  gATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCaaaaa                                                                                                      >  2:872862/1‑151 (MQ=255)                                    taataCCACTCACTGAGAGTGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATa                                                                                                    >  2:704948/1‑151 (MQ=255)                                    taataCCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATa                                                                                                    >  1:834812/1‑151 (MQ=255)                                      ataCCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGt                                                                                                  >  2:809810/1‑151 (MQ=255)                                        aCCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGtat                                                                                                <  2:815292/151‑1 (MQ=255)                                        aCCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGtat                                                                                                <  2:1440778/151‑1 (MQ=255)                                        aCCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGtat                                                                                                >  2:184689/1‑151 (MQ=255)                                          cactcactGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATAc                                                                                              <  2:636077/151‑1 (MQ=255)                                          cactcactGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATAc                                                                                              <  2:34562/151‑1 (MQ=255)                                            ctcactGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACtt                                                                                            <  2:799174/151‑1 (MQ=255)                                             tcactGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACttt                                                                                           <  2:1174542/151‑1 (MQ=255)                                              cactGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTa                                                                                          >  1:725029/1‑151 (MQ=255)                                                   agaATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTcc                                                                                      <  1:813302/150‑1 (MQ=255)                                                    gaATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCag                                                                                    >  2:1475908/1‑151 (MQ=255)                                                       tGATAAAATTACAAAAAAGAATAAAGACAGCAAAGGACCCGCAACATGAAACATATCCATTTGTTTTC‑AAAAAAAAATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATGGTTTATTCCagaag                                                                                 <  1:953789/151‑1 (MQ=255)                                                       tGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCANNNNNNNTCCAAAAANNNTATACTTTATNCCagaag                                                                                 >  1:52652/1‑151 (MQ=255)                                                       tGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCagaag                                                                                 <  1:599179/151‑1 (MQ=255)                                                         aTAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAagag                                                                               <  1:100276/151‑1 (MQ=255)                                                               ttACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAat                                                                         <  2:1431612/151‑1 (MQ=255)                                                                 aCAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATAACCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAAAATCCAAAAATAGTATACTTTATTCCAGAAGAGTGCAatat                                                                       <  1:372630/151‑1 (MQ=255)                                                                      aaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGt                                                                  <  1:1016840/151‑1 (MQ=255)                                                                      aaaGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGt                                                                  <  1:1368146/151‑1 (MQ=255)                                                                         gAATGAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTg                                                               >  2:1253447/1‑151 (MQ=255)                                                                          aaTAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGt                                                              <  2:418702/151‑1 (MQ=255)                                                                           aTAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTc                                                             <  1:195575/151‑1 (MQ=255)                                                                           aTAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTc                                                             <  1:1237585/151‑1 (MQ=255)                                                                                  cAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAAtt                                                      >  1:612665/1‑151 (MQ=255)                                                                                  cAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAAtt                                                      >  1:1052519/1‑151 (MQ=255)                                                                                    gCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAAtttt                                                    >  2:363966/1‑151 (MQ=255)                                                                                      aaaGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTc                                                  <  2:338615/151‑1 (MQ=255)                                                                                             cTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTc                                           <  2:867259/151‑1 (MQ=255)                                                                                                  aCATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGt                                      <  1:970724/151‑1 (MQ=255)                                                                                                   cATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTa                                     >  1:951711/1‑151 (MQ=255)                                                                                                       aaaaaTATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGTAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAatat                                 <  1:1436574/151‑1 (MQ=255)                                                                                                       aaaaaTATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAatat                                 <  1:1283356/151‑1 (MQ=255)                                                                                                       aaaaaTATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAatat                                 <  2:68767/151‑1 (MQ=255)                                                                                                       aaaaaTATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAatat                                 <  1:1050544/151‑1 (MQ=255)                                                                                                         aaaTATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAg                               <  2:1323012/151‑1 (MQ=255)                                                                                                         aaaTATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAg                               <  2:79439/151‑1 (MQ=255)                                                                                                         aaaTATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAg                               >  2:1502634/1‑151 (MQ=255)                                                                                                         aaaTATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAg                               <  2:699984/151‑1 (MQ=255)                                                                                                         aaaTATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAg                               <  1:1072908/151‑1 (MQ=255)                                                                                                           atatCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGAt                             <  2:1445031/151‑1 (MQ=255)                                                                                                           atatCCATATGTTTAC‑AAAAAAAGATTATTAAGAAGGGAATTAATGCAATTATCGAAAAGTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGTGTAATATAGAt                             <  1:704545/151‑1 (MQ=255)                                                                                                             atCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTg                           >  1:1322300/1‑151 (MQ=255)                                                                                                             atCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTg                           >  1:706426/1‑151 (MQ=255)                                                                                                                cATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATGATCGAAAATTCAAAAAATATCCAAAAATAGTATGCTTTATTCCAGAAGAGTTCAAGATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTCCTc                        <  1:1048321/151‑1 (MQ=255)                                                                                                                      tttgCAAAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTAc                   <  2:1505605/151‑1 (MQ=255)                                                                                                                      tttgC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTan                   <  2:52679/150‑2 (MQ=255)                                                                                                                      tttgC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTAc                   <  2:1193709/150‑1 (MQ=255)                                                                                                                      tttgC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTAc                   <  2:1197156/150‑1 (MQ=255)                                                                                                                      tttgC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTAc                   <  1:823146/150‑1 (MQ=255)                                                                                                                      tttgC‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTAc                   <  1:409346/150‑1 (MQ=255)                                                                                                                          c‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATtg               <  1:575130/150‑1 (MQ=255)                                                                                                                          c‑AAAAAAAGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATtg               <  2:1151226/150‑1 (MQ=255)                                                                                                                            aaaaaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATtgtg             <  2:127839/151‑1 (MQ=255)                                                                                                                               aaaaGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGc          <  1:1278645/151‑1 (MQ=255)                                                                                                                                  aGATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCtt        >  1:335104/1‑150 (MQ=255)                                                                                                                                   gATTATTAGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCa      >  2:762715/1‑151 (MQ=255)                                                                                                                                     ttattaGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCAt     >  2:1164986/1‑150 (MQ=255)                                                                                                                                     ttattaGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATc    <  1:1079145/151‑1 (MQ=255)                                                                                                                                       attaGGAAGGGAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCtt  >  2:517186/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                             ACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGAGAATGATAAAATTACAAAAAAGAATAAAGACAGCAAAGAACCTTCAACATGAAAAATATCCATTTGTTTGC‑AAAAAAAGATTATTAGGAAGGAAATTAATGCAATTATCGAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTT  >  NC_000913/1197596‑1197879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑