Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,826,379	0	T	C	51.2%	24.8 / 52.1	43	E182G (GAG→GGG)	astE	succinylglutamate desuccinylase
Reads supporting (aligned to +/- strand):  ref base T (13/8);  new base C (0/22);  total (13/30)
Fisher's exact test for biased strand distribution p-value = 5.56e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.23e-04

CTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGTTCC‑AGCGTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTAAA‑CGTACCACCAGGTTCCTGATGGAA‑CACCAGCGCCTCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCC  >  NC_000913/1826235‑1826519                                                                                                                                                    |                                                                                                                                             cTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGTTCC‑AGCGTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTAAA‑CGTACCACCAGGTTCCTGATGGAA‑CACCAGCGCCTCCAGcc                                                                                                                                        <  2:193196/151‑1 (MQ=255)           cTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGTTCC‑AGCGTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTAAA‑CGTACCACCAGGTTCCTGATGGAA‑CACCAGCGCCTCCAGCCCCGCCGCAc                                                                                                                               >  2:329857/1‑150 (MQ=255)                              tttttGCCCAAAGGCCACCGTTTGGCAAAGTCCC‑GGCGTACAGGCCAGCGCGCCAAAATGTTGGGCGTTAAATTGGGTAAA‑CGTCCCCCCAGTTTCTTGATGGAA‑CCCCAGCGCCCCCACCCCCGCCGCACCCAGCCACGTCAGAAATTtc                                                                                                           <  2:400079/150‑1 (MQ=255)                                   gCCCAAAGGGCAACGCTTTGCCAAGTTCC‑AGCGTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTAAA‑CGTACCACCAGGTTCCTGATGGAA‑CACCAGCGCCTCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTTGt                                                                                                       >  1:734333/1‑150 (MQ=255)                                   gCCCAAAGGGCAACGCTTTGCCAAGTTCC‑AGCGTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTAAA‑CGTACCACCAGGTTCCTGATGGAA‑CACCAGCGCCTCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGt                                                                                                       >  1:245801/1‑150 (MQ=255)                                           gccacggttttGCAAATTCCC‑AGCGAACGGGCCAGCGCGCCAAAATGCCGGGCGCT‑AAAAGGGTAAACCGCCCCACCAGGTTCCGGAGGAAC‑CCCCAGCCCCCCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAggg                                                                                                <  1:255172/141‑1 (MQ=255)                                              caccgttttgcAAGTTCCAAGCGAACGGGCCAGCGCGCAAAAATGTCGGGCGCGGAAATGGGTAAC‑CGTCCCCCCGGGTCCCGGATGAAA‑CCCCGGCCCCCCCGGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAAt                                                                                             <  1:375291/140‑1 (MQ=255)                                                cGCTTTGCCAAGTTCC‑AGCGTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTAAA‑CGTACCACCAGGTTCCTGCTGGAA‑CACCAGCGACTCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTTCCGTCCCAGGGaaactcg                                                                                         >  1:358859/1‑146 (MQ=255)                                                            ttCC‑AGCGTACAGGCCAGCGCGCAAAAATGTCTGGCGCGAAAATGGGTAAC‑CGTACCCCCAGGTTCCTGATGAAA‑CCCCAGCGCCCCCACCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTaa                                                                              <  2:376623/150‑1 (MQ=255)                                                             ccc‑AGCGAACAGGCCAGCGCGAAAAAATGTCTGCCGCTGAAAGGGGAAAA‑CGTACCACCAGTTCCCTAATGGAA‑CACCAGCGCCTCCGCCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATa                                                                            <  1:771612/150‑1 (MQ=255)                                                              cc‑AGCGTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTAAA‑CGTACCACCAGGTTCCTGATGGAA‑CACCAGCGCCTCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATa                                                                            >  1:140511/1‑150 (MQ=255)                                                              cc‑AGCGAACGGGCCGGCGCGCAAAAAGGTCTGGCGCTAAAATGGGAAAA‑CGCCCCCCCAGGTTCCTGGTGGAA‑CCCCAGCGCCCCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATac                                                                           <  2:755172/151‑1 (MQ=255)                                                              cc‑AGCATAAAGGCGAGGGCGAAAAAATGTCTGGCGCTGAAATGGCAAAA‑CGTCCCACCAGTTTCCTGGGGGAA‑CACCCGCGCCCCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATac                                                                           <  1:226338/151‑1 (MQ=255)                                                                   cGTACGGGCCGGCGCCCCAAAATGTCTGGCGCAGAAAGGGGAAAA‑CGTCCCACCAGGTCCCGGAGGAAA‑CCCCGGCCCCTCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATacac                                                                         <  2:252320/149‑1 (MQ=255)                                                                   cGTACAGCCAAGCGCGCAAAAATGTCGGGCGC‑GGAATGTGTAAACCGTCCCCCCAGGTTCCTGATGGAA‑CCCCCGCACCTCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATacac                                                                         <  1:259396/149‑1 (MQ=255)                                                                    gTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTAAA‑CGTACCACCAGGTTCCTGATGGAA‑CACCAGCGCCTCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGa                                                                      >  1:669761/1‑151 (MQ=255)                                                                    gTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTAAA‑CGTACCACCAGGTTCCTGATGGAA‑CACCAGCGCCTCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGa                                                                      >  2:200933/1‑151 (MQ=255)                                                                    gTACAGGCCAGAGCGCCAAAATAGCTGGCGCTGTAATTGGTAAA‑CGTACCACCCAGATCCTGCTGGAA‑CACCATCGCCTCCAGCCCCGCCGCACCCAGCCGAGTTAGAAATAACTCGTCCCCGGGAGTGTCACTTGAACGTAATACACCGa                                                                      >  1:781025/1‑151 (MQ=255)                                                                                 cgcAAAAAGGTCGGGCGCTAAAAGGGGTAAA‑CGTCCCCCCAGTTCCCTGAGGGAA‑CCCCAGCGCCCCCACCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCa                                                          <  2:376758/150‑1 (MQ=255)                                                                                  gcCAAAATGTCTGGCGCTGAAATGGGTAAA‑CGTACCCCCAGGTTCCTGATGGAA‑CACCAGCGCCTCCAGCCCCGCCGCACCCAGCCACGTCCGAAATCTCTCGTCCCAGTGGATGTCGCGTTGCGGTAATCCACCGAACTCCCGAtcccag                                                        >  1:207631/1‑146 (MQ=255)                                                                                   caaaaaTGTCGGGCGCAAAAAGGGGTAAA‑CGTCCCCCCGGTTTCCTGAGGGAA‑CCCCAGCGCCCCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAgg                                                       <  2:191541/149‑1 (MQ=255)                                                                                   caaaaaGGTCGGGCGCTAAAATGGTTAAC‑CGTCCCACCAGGTTCCGGATGGAA‑CACCGGCGCCCCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAgg                                                       <  2:457040/149‑1 (MQ=255)                                                                                          ctctgcggcagAAAGGGGTAAC‑CCCACCCCCGGGTTCCTGAGGGAA‑CCCCGGCGCCTCCGGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGACCTGCGGATGCAAGGAGCCACg                                                <  1:460697/141‑1 (MQ=255)                                                                                           tcgggCGCTAAAAGGGGAAAA‑CGTCCCCCCAGTTTCCTGATGAAA‑CCCCGGCCCCCCCACCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGa                                               <  2:807469/148‑1 (MQ=255)                                                                                           tcgggCCCTAAAATGGGTAAA‑CGTCCCCCCAGGTTCCTGATGAAA‑CCCCGGCGCCCCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACg                                                <  2:456073/147‑1 (MQ=255)                                                                                                         gggTAAA‑CGTACCACCAGGTTCCTGATGGAA‑CACCAGCGCCTCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGCTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGCATTGCGGTATGTAg                                 >  1:451276/1‑151 (MQ=255)                                                                                                           ttAAA‑CGCACCACCAGTTTCCTGAGGGAA‑CACCAGCGCCTCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGa                                <  1:62710/149‑1 (MQ=255)                                                                                                           gaaaa‑CGTCCCCCCAGGTTCCTGAGGAAA‑CCCCAGCGCCCCCACCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGa                                <  2:355391/148‑1 (MQ=255)                                                                                                            taaaccGTCCCCCCAGGTCCATGATGAAA‑CCCCAGCCCCCCCCGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGa                                <  1:514825/145‑1 (MQ=255)                                                                                                            tAAA‑CGGCCCCCCAGGTTCCGGAGGGAA‑CACCAGCGCCCCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATc                              <  2:636450/151‑1 (MQ=255)                                                                                                            gaaaccgcccccccaGTTCCCTGATGAAA‑CCCCAGCCCCTCCGGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGa                                <  1:366980/138‑1 (MQ=255)                                                                                                               a‑CGTACCACCAGGTTCCTGATGGAA‑CACCAGCGCCTCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAg                           >  2:622051/1‑151 (MQ=255)                                                                                                                 cttccccccaGGTTCCTGAGGGAA‑CACCAGCGCCCCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAgg                          <  2:403856/144‑1 (MQ=255)                                                                                                                 cgcaccacgAGGTGCCTGATAAAA‑CCCGGGCACCCAAAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAg                           <  1:675259/147‑1 (MQ=255)                                                                                                                 cGTCCCCCCGGGTTCCTGATGAAC‑CCCCAGCGCCCCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAgg                          <  2:616955/151‑1 (MQ=255)                                                                                                                 cGTCCCCCCAGTTCCCGGAGGGAA‑CCCCAGCCCCCCCACCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAgg                          <  2:500485/151‑1 (MQ=255)                                                                                                                 cGTCCCCCCAGGTTCCTGATGGAA‑CCCCAGCGCCCCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAgg                          <  2:670063/151‑1 (MQ=255)                                                                                                                     ccaccaGGTTCCTGATGGAA‑CACCAGCGCCTCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGc                       >  2:185170/1‑150 (MQ=255)                                                                                                                      cccccggTTCCTTAAGGAACCCCCAGCCCCCCCGCCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAGTTGCGGTATGTAGATCAAGGTGcc                      <  2:212233/146‑1 (MQ=255)                                                                                                                            gTTCCTGATGGAA‑CACCAGCGCCTCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGca                >  2:334068/1‑150 (MQ=255)                                                                                                                                  gaggaaa‑CCCCGGCCCCCCCACCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGAtt          <  2:184974/145‑1 (MQ=255)                                                                                                                                      aaa‑CCCCCGCGCCCCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTg     <  2:134434/150‑1 (MQ=255)                                                                                                                                          cacCGGCCCCTCCCGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGccc  <  1:195971/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                             CTGGCAGTTACTGCAAACTGGCGAAGATCGTTTTGCCCAAAGGGCAACGCTTTGCCAAGTTCC‑AGCGTACAGGCCAGCGCGCCAAAATGTCTGGCGCTGAAATGGGTAAA‑CGTACCACCAGGTTCCTGATGGAA‑CACCAGCGCCTCCAGCCCCGCCGCACCCAGCCACGTCAGAAATTTCTCGTCCCAGGGAATGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCC  >  NC_000913/1826235‑1826519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑