Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,640,9810CT48.7% 17.1 / 23.3 39F40F (TTC→TTT) dtpBdipeptide and tripeptide permease B
Reads supporting (aligned to +/- strand):  ref base C (6/14);  new base T (19/0);  total (25/14)
Fisher's exact test for biased strand distribution p-value = 3.34e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGA  >  NC_000913/3640837‑3641122
                                                                                                                                                 |                                                                                                                                             
ggATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGGTTTCTTCttt                                                                                                                                         >  1:200402/1‑151 (MQ=255)
    ggTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTtaaa                                                                                                                                     >  2:803925/1‑150 (MQ=255)
    ggTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGGtttttttttttaaa                                                                                                                                     >  2:366510/1‑143 (MQ=255)
     gTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTtaaaa                                                                                                                                    >  2:516141/1‑149 (MQ=255)
     gTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTaaaaa                                                                                                                                    >  2:370078/1‑149 (MQ=255)
     gTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTTAACa                                                                                                                                    >  2:485741/1‑151 (MQ=255)
     gTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTTGTTTAACa                                                                                                                                    >  2:460601/1‑151 (MQ=255)
     gTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGttttaca                                                                                                                                    >  1:570260/1‑146 (MQ=255)
     gTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAAc                                                                                                                                     <  2:160343/150‑1 (MQ=255)
       aTTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTCGTTTAAAAg                                                                                                                                   >  2:465470/1‑150 (MQ=255)
               gTTAATAAATATGAATACAACATCACCCATGGGGATGCTTCCGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGTGCGTCCTGGC‑GCTTTTCTTTCTTCAACAACTTggggtt                                                                                                                          >  1:495712/1‑147 (MQ=255)
               gTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTGTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTTGGAtt                                                                                                                           <  1:348854/150‑1 (MQ=255)
                        tatGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTAAAAAGCCTGGGTTTTCGCCagag                                                                                                                 >  1:442990/1‑151 (MQ=255)
                        tatGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTCCTTAAACAACTTGGGTTTTTGCAagag                                                                                                                 >  2:334600/1‑151 (MQ=255)
                         atGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGc                                                                                                                <  2:540500/151‑1 (MQ=255)
                           gAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTAAAAAACTTTGGTTTTCTCCAGAGCAg                                                                                                              >  2:32207/1‑151 (MQ=255)
                           gAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGGTTTTTTCGTTAAAAAACTTGGGTTTTCGcgaagacag                                                                                                              >  1:580474/1‑143 (MQ=255)
                           gAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTACAGGGCGTACTGGCGGGTTTTTTTCGTTAAA‑AACTTTGGTTTTCCCAAGAACAg                                                                                                              >  2:490282/1‑151 (MQ=255)
                                      cacCCATGGGGACGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGACTTTTTCGTTAAACAGCTTTGACTCCCGCAAAAGGAGGCTTTTGTcc                                                                                                    >  2:689789/1‑149 (MQ=255)
                                       acCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTCGTTTAAAAGCTTGGGTTTTCCCCAAAGCAGGCgttttttt                                                                                                    >  1:232276/1‑145 (MQ=255)
                                        cccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTTAACAACTTGGAGTCTCGCCCAAGCACGCTTTTTTCGCtt                                                                                                 >  1:384125/1‑151 (MQ=255)
                                        cccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGGTTTCTTTGTTTAAAAACTTTGGTTCTCGCCAGAGCCGGCtttttttcc                                                                                                   >  2:475462/1‑144 (MQ=255)
                                              gggATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTTAAAAACTTGGGTTTTTGCCAGAGCAGGGTTTTTTTACtttttggg                                                                                           >  2:579128/1‑147 (MQ=255)
                                               ggATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTg                                                                                           <  1:366317/150‑1 (MQ=255)
                                                 aTGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTTAAAAGCTTTGGTTTTCGCCAGAGGAGGCTTTTTTTACTTTTTGTGGtt                                                                                        >  2:579780/1‑151 (MQ=255)
                                                          caACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTgcgc                                                                               <  2:548875/151‑1 (MQ=255)
                                                                   ccATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATg                                                                      <  2:759860/151‑1 (MQ=255)
                                                                    cATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATgg                                                                     <  1:118233/151‑1 (MQ=255)
                                                                          tCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTTAAAAAGTTGGGTTTTCGCAAAAGCCGGGTTTTGTCCTTTTTTGTGGTTTTTCTTCGCTGGGCTTTGGGCTctt                                                               >  2:545569/1‑149 (MQ=255)
                                                                           cATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCAtt                                                              <  1:155228/151‑1 (MQ=255)
                                                                            aTGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTGCTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCAttt                                                             <  2:144145/151‑1 (MQ=255)
                                                                                tCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCAt                                                         <  2:433004/151‑1 (MQ=255)
                                                                                           gAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGTTAAAAAACTTTGGTTTTCCCAAAAGCAGGGTTTTTTCACTTTTTGGGCTTTTTCTGCGCCGGGCTATTGCCCCCttttcctttgggggtatt                                              >  2:728389/1‑136 (MQ=255)
                                                                                           gAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTGGTAAAAAGCTTTGGTTCTCGCAAAAGGAGGGTTTTTTCTCTTTTGGGGCTTTTTGCGCGCCGGGCTATGGCCTCCTTTTCAtttgggggg                                                 >  2:112597/1‑141 (MQ=255)
                                                                                                             ggCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTTTTTCTTAAAAAGCCTGGATTCTCCCCAGAACAGGGTTTTTTTCCTTTTTGGGGTTTTTCTTCCCCGGTCTCTGGGCCCCTTTTCCTTGGCGGGTTTGTTgggggcccccccccgg                            >  2:61208/1‑137 (MQ=255)
                                                                                                                       gCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAAc                   <  2:38111/150‑1 (MQ=255)
                                                                                                                                   tACTGGC‑GGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTc       <  2:608714/150‑1 (MQ=255)
                                                                                                                                   tACTGGC‑GGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTc       <  1:369881/150‑1 (MQ=255)
                                                                                                                                    aCTGGC‑GGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCtt     <  1:233084/151‑1 (MQ=255)
                                                                                                                                       ggC‑GGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCCGCTGGGGGCCAAAAGCACCATTGTTCTTGGa  >  1:547491/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
GGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGTCGGCGACCACCTGCTGGGGACCAAACGCACCATTGTTCTTGGA  >  NC_000913/3640837‑3641122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑