Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913281,0890AC53.2% 55.3 / 74.0 48Y299D (TAT→GAT) yagACP4‑6 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (17/5);  new base C (0/25);  total (17/31)
Fisher's exact test for biased strand distribution p-value = 9.61e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-08

GTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGT  >  NC_000913/280945‑281232
                                                                                                                                                  |                                                                                                                                               
gTTCTCCCCTGAACGCCTTGCCTGCGCCCAGACTTACCCCTTTCCCGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGCAGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGa                                                                                                                                           >  1:671721/1‑151 (MQ=255)
     ccccTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCg                                                                                                                                      >  2:4043/1‑151 (MQ=255)
         tGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCAGGTAc                                                                                                                                  <  2:13041/151‑1 (MQ=255)
               ccTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCat                                                                                                                            >  1:596865/1‑151 (MQ=255)
               ccTTGCCTGCCCTCAGACTTCCCCCTTCCCCGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCCCCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCa                                                                                                                             <  2:390247/150‑1 (MQ=255)
                   gCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATc                                                                                                                         >  2:331356/1‑150 (MQ=255)
                    ccggcgcCCAGACTTCCCCCTTCCCCCCCCAGTTTTCCGTGGATATCCCCTTCCCGGCCCTCCCCCCCTTCTTCGTATCCGGGGGGCGTGGTGTTGCCGCTGTCCTCCCGCGCTAGCGGCTGATCCCGCGAGCCCGGTACCGCCATAGcc                                                                                                                        <  1:263133/147‑1 (MQ=255)
                    ccTGCGCTCAGACTTCCCCCTTTCCCGCTCAGCTTTCCGCTGATATCCCCTTTCCTGACCACCCCCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATcc                                                                                                                        <  2:65470/150‑1 (MQ=255)
                       gcgcTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAgcg                                                                                                                    >  2:132156/1‑151 (MQ=255)
                        cgcTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAgcg                                                                                                                    >  2:450703/1‑150 (MQ=255)
                        cgcTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAgcg                                                                                                                    >  1:10443/1‑150 (MQ=255)
                              gACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTg                                                                                                             >  2:331363/1‑151 (MQ=255)
                                           cgcgttccccTTCCCGCTTATACCCCTTTTCCTGACAATCCCCCCTCAATCGAATTCGGGAGGCGTTTTGTTGCCGCTTTCCTCCCGGGCGAGCGGCAGAACCCGCGAGGCGGTTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAgg                                                                                                <  1:387108/142‑1 (MQ=255)
                                            ccccccatcTTCCCGCTGATTTCCACTTTCCTGCCCATCCCCCCTTCAGCGTATCCGGGGGGCTTTGTGTTCCCGCTTTACTGCCGCGCTGGCGGCTGATCCCGCGGGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGt                                                                                               <  1:591503/143‑1 (MQ=255)
                                                 cggCTTCCCGCTTATCTCCCCTTCCCTGCCCACCCCCCCTTCATCGTATTCCGGGGGCTTTGTGTTGCCGCTGTACTGCCGCGCTGCCGGCTGATCCCGCGAGCCCGGTCCCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAg                                                                                          <  1:4042/149‑1 (MQ=255)
                                                   tcTTTCCGCTGTTTCCCACTTCCCTGCCCACCCCCCTTGCATCTTATCCCGGGGGCTTGGTTTTCCCTCTGTCCGGCCGCGCGGCCGGGTGATCCCGCGAGCCGGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGAc                                                                                        <  1:744710/150‑1 (MQ=255)
                                                   ctttcccgcgaatatCCACTTCCCTACCCATCCCCCCTCAATGTTATCCGGGGGGCTTGTGGTTGCCGCTCAACCGCCGCGCGGAGGGGTGATCCCGCGCGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGAc                                                                                        <  1:464436/140‑1 (MQ=255)
                                                   ctgtccctctaatttCCCCTTTCCTGACCCCCCCCCCTCACGCGTTTTCGGGGGGCGTGGTGTTGCGGCTGTACTCCGGCGCTAAGGCCGGTGCCCGCGAGCCGGTTGCCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGAc                                                                                        <  1:679554/137‑1 (MQ=255)
                                                   ccTTTCCGCTGATACCCCCTTCCCGGCCCCCCCCCCCTCCATGTTTTTCCGGGGGGTTTGTTTTGCCGCTTTACCCCCGCGCTGCCGGTTGATCCCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGAc                                                                                        <  2:245134/150‑1 (MQ=255)
                                                   ccTTCCCGCTGATATCCCCTTTCCTGCCCACCCCCCCTCCAGCGTATTCGGGGGGCGTTGTTTTGCCGCTGTCCTCCCGCGCTGACGGCTGATCCCGCGACCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGAc                                                                                        <  2:583978/150‑1 (MQ=255)
                                                   ccTTCCCGCGGATACCCTCTTCCCCGACCCCCCCCCCTTCATCTTATTCGGGGGGCGTTGTGTGCCCGCTGTCCTGCCGCGCGGACGGCTGATCCCGGGGGCCGGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGAc                                                                                        <  1:670253/150‑1 (MQ=255)
                                                    ttttCCGCTGATACCCCCTTCCCTGCCCACCCCCCCTTCATCGTATCCGGGGGGCGTTGTTTTGCCGCTGTACTGCCGCGCTGACGGTTGATCCCGCGAGCCCGGTCCCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGAc                                                                                        <  2:695717/149‑1 (MQ=255)
                                                    tttcccGCTTACACCCGCTTTCCCCACCACCCCCCCTTCATTGTTTTCGGGGGGCGTTGTGTGGCCGCTGGACCCCGGCGCTGCCGGTTCTTTCCGCGAGCCGGTTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGAc                                                                                        <  2:24972/146‑1 (MQ=255)
                                                    cTTTCCGTGGATATCCACTTCCCTGACCACCCCCCCTTCATGGTATTCCGGGGGCGTTGTGTTGCCGCTGTACGGCCGCGCTGACGGCTGATCCCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGAc                                                                                        <  2:630229/150‑1 (MQ=255)
                                                            taggtcccACTTTCCGGACCACCCCCCTTTCACCGTCGCCGGGGGGCGTTGTTTTGCCGGTGTCTTGCGGCCCGGGCGGTTGATCCCGCGCCCCCGTTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGcc                                                                                <  1:780756/144‑1 (MQ=255)
                                                             gATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAg                                                                              >  2:335336/1‑151 (MQ=255)
                                                                  cctcTTTCCTACCCACCCCCCCTTCATCGTATCCGGGGGGCTTTGTGTTGCCGCTGTCCTGCCGCGCTGACGGTTGATCCCGCGAGCCCGTTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTc                                                                         <  1:591368/148‑1 (MQ=255)
                                                                         ccTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGgcg                                                                  >  1:262912/1‑151 (MQ=255)
                                                                                    ccccTTCATCGTTTCCGGGGGGCGTTGTGTGGCCGCTGTCCTGCCGCGCTGGCGGCTGATCCCGCGGGCCCGGTCCCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTc                                                       <  2:116956/151‑1 (MQ=255)
                                                                                    ccccTTCATCGTTCCCGGGGGGCGTGGTGTCCCCGGTGTACTGCGGCGCTGGGGGCGGATCCCGCGAGCCCGTTGCCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGGCCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTc                                                       <  1:195750/151‑1 (MQ=255)
                                                                                    ccccTCCATCGTATCCGGGGGGGTTTGTGTGGCCGCTGTACGGCCGGGCGGACGGCGGATCCCGCGAGCCGGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTc                                                       <  2:783227/151‑1 (MQ=255)
                                                                                         tCACCGTATCCGGGGGGCTTTGTTTGGCCGCTGTCCTGCCGGGCGGCGGGCTGATCCCGGGACCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGc                                                  <  2:111351/151‑1 (MQ=255)
                                                                                         ccttcttatccgggggggGTTGTGTGGCGGTTGGCCTCCCGGGCTGACGGCGAACACGGCGCCCCGGGACCCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGc                                                  <  1:107024/139‑1 (MQ=255)
                                                                                         ccagcttttcccGGGGGCGTTTTTTTGCCGCTGTACTGCCGGGGTGAGGGCTGATCCCGGGAGCCGGGACCCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGc                                                  <  2:753150/141‑1 (MQ=255)
                                                                                               tatccggggggCGTTGTTTTGCCGCTGTCCTGCCGCGCTGAGGGCTGATACCGCGAGCCCGGTCCCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTg                                            <  2:328136/145‑1 (MQ=255)
                                                                                                tatCCGGGGGGGTTTGTGTTCCCCCTGTCCTCCCGCGCGGGCGGCTGATCCCGCGCGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTg                                            <  1:331807/149‑1 (MQ=255)
                                                                                                     gggggCGTTGTGTGCCCCCTGTACGCCCGCGCTGCCGGCTGACCCCGCGAGCCAGGTGCCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAAc                                       <  1:16933/150‑1 (MQ=255)
                                                                                                       gggCGTTGTTTTGCCGCTGTACTGCCGCGCGGACGGCTGATCCCGGGAGCCGGTTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCa                                     <  2:102326/150‑1 (MQ=255)
                                                                                                        ggCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGTGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCAtt                                   >  1:84387/1‑151 (MQ=255)
                                                                                                           gTTGGGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGCAGGCGCGCCGCAGTTCACCGCTGTCTGCGAACCAtttt                                 >  1:759186/1‑150 (MQ=255)
                                                                                                              tgtgTTCCCCCTTTCCGGCCGCGCTGAGGGCGGATCCCGGGGCCCCGGTCCCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTccc                              <  1:314007/151‑1 (MQ=255)
                                                                                                               gtgtTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGc                                           >  2:63328/1‑137 (MQ=255)
                                                                                                                tttttcccGCTGTCCCGCCGCGCTGCCGGCTGATCCCGCGACCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGc                                           <  1:63321/131‑1 (MQ=255)
                                                                                                                      ccGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTgcagca                       >  1:23005/1‑151 (MQ=255)
                                                                                                                           gTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTc                   >  2:85810/1‑150 (MQ=255)
                                                                                                                                     gcTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAgg         >  2:122805/1‑150 (MQ=255)
                                                                                                                                      cTGCCGGCTGATCCCGCGACCCGGGTTCCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGc        <  2:625868/150‑1 (MQ=255)
                                                                                                                                           ggCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGt  >  2:456270/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGT  >  NC_000913/280945‑281232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: