Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,189,448 C→T Q1367* (CAG→TAG)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,189,4480CT98.0% 184.2 / ‑4.1 50Q1367* (CAG→TAG) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (20/29);  total (21/29)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

TCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTA  >  NC_000913/4189301‑4189595
                                                                                                                                                   |                                                                                                                                                   
tCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAgg                                                                                                                                                  >  1:170272/1‑151 (MQ=255)
tCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAgg                                                                                                                                                  >  2:429620/1‑151 (MQ=255)
 ccTTCATCTCCGCGGCATCGTTCCAGGAGAGCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGa                                                                                                                                                 <  2:319111/151‑1 (MQ=255)
 ccTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGa                                                                                                                                                 <  2:787786/151‑1 (MQ=255)
 ccTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGa                                                                                                                                                 <  2:672838/151‑1 (MQ=255)
 ccTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGa                                                                                                                                                 <  2:514522/151‑1 (MQ=255)
 ccTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGa                                                                                                                                                 <  2:11330/151‑1 (MQ=255)
  cTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGAt                                                                                                                                                <  2:809266/151‑1 (MQ=255)
  cTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGAt                                                                                                                                                >  2:370314/1‑151 (MQ=255)
  cTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGAt                                                                                                                                                <  1:268060/151‑1 (MQ=255)
  cTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGAt                                                                                                                                                <  2:302453/151‑1 (MQ=255)
  cTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTAACCGAAGCAGCCGTTGCGGGCAAACGCTACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCTTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGAt                                                                                                                                                <  2:172432/151‑1 (MQ=255)
    tCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCg                                                                                                                                              <  1:312801/151‑1 (MQ=255)
      aTCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTa                                                                                                                                            <  2:251997/151‑1 (MQ=255)
      aTCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTa                                                                                                                                            <  1:802339/151‑1 (MQ=255)
        ctcCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATg                                                                                                                                          <  2:43834/151‑1 (MQ=255)
               gCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGc                                                                                                                                    >  1:642416/1‑150 (MQ=255)
                     ttCCTGGAGACCACTCGCGTGCTGACGGTAGCAGCCGTTTCGGGCAAACGCGACGAACCGCGCGGCCTGAAAGAGAACGTTATCGTGGGGGGTCTGACGCAGGCAGGTACCGGCTACGCGTCCCAATAGGAACGGATCCGTCTTCGtgct                                                                                                                              >  1:807266/1‑150 (MQ=255)
                     ttCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAGCGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGtg                                                                                                                                >  1:251971/1‑148 (MQ=255)
                     ttCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGtg                                                                                                                                >  1:298664/1‑148 (MQ=255)
                                 aCTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTc                                                                                                                 <  2:356376/151‑1 (MQ=255)
                                                 aaGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAgg                                                                                                  <  1:700063/150‑1 (MQ=255)
                                                 aaGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAgg                                                                                                  <  1:177501/150‑1 (MQ=255)
                                                    cagcCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTg                                                                                                <  2:140910/149‑1 (MQ=255)
                                                             cGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCagaag                                                                                      <  1:186474/150‑1 (MQ=255)
                                                             cGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCagaag                                                                                      <  2:507775/150‑1 (MQ=255)
                                                                       cgacgaACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGcc                                                                           <  2:659091/151‑1 (MQ=255)
                                                                                     gCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGaa                                                               >  2:92760/1‑149 (MQ=255)
                                                                                     gCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGaa                                                               >  2:658080/1‑149 (MQ=255)
                                                                                     gCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGaa                                                               >  1:255260/1‑149 (MQ=255)
                                                                                               gaACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCGGGGGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCa                                                   >  2:175740/1‑151 (MQ=255)
                                                                                               gaACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGGGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCa                                                   >  2:22692/1‑151 (MQ=255)
                                                                                                 aCGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAgg                                                 >  1:637882/1‑151 (MQ=255)
                                                                                                  cGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGt                                                >  2:310547/1‑151 (MQ=255)
                                                                                                   gTTATCGTGGGTCGTCTGATCCCGGCAGGGACCGGTTACGCGTACCAATAGGATCGTATGTGTCGCCGTGCTGCGGGGGAAGCTCCGGCTGCACCGCAGGGGACTGCAGAAGCAGCATCAGCCAGCCTCGCAGAACAGCTGAACGCAGGt                                                >  1:707101/1‑150 (MQ=255)
                                                                                                    ttATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTc                                               <  2:511698/150‑1 (MQ=255)
                                                                                                      aTCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTgg                                            >  2:163947/1‑151 (MQ=255)
                                                                                                        cGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGc                                          <  2:376614/151‑1 (MQ=255)
                                                                                                        cGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGc                                          <  2:638167/151‑1 (MQ=255)
                                                                                                             gtcgtcTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGTTGAACGCAGGTCTGGGCGGtt                                      >  1:258225/1‑150 (MQ=255)
                                                                                                              tcgtcTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTc                                     <  2:492822/150‑1 (MQ=255)
                                                                                                                  cTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATa                                <  2:765893/151‑1 (MQ=255)
                                                                                                                  cTGATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATa                                <  2:552639/151‑1 (MQ=255)
                                                                                                                    gATCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATaa                               >  1:298989/1‑150 (MQ=255)
                                                                                                                      tCCCGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGGGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGa                            >  2:349063/1‑151 (MQ=255)
                                                                                                                       cccGGCAGGTACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGCACGCAGGTCTGGGCGGTTCTGATAACGAg                           >  1:807090/1‑151 (MQ=255)
                                                                                                                                tACCGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGtt                   <  2:497658/150‑1 (MQ=255)
                                                                                                                                   cGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATc               <  2:80479/151‑1 (MQ=255)
                                                                                                                                   cGGTTACGCGTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATc               <  2:339377/151‑1 (MQ=255)
                                                                                                                                         cgcgTACCACTAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCaaa         >  1:591336/1‑151 (MQ=255)
                                                                                                                                             tcccactaGGATCGAATGCGCCGCCGTGCGGCGGGTGAAGACGAGGCTTCACCGCAGGTGCCTGCAGAAGACGCATCTGCCAGACTGGCAGAATTGCTGCACGCAGGTCTGGGTGGTTCTGATAACGATTCATCGTTAATACGCAACTag      >  1:444717/3‑149 (MQ=255)
                                                                                                                                               ccactaGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGt   >  2:546123/1‑151 (MQ=255)
                                                                                                                                               ccactaGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGt   >  1:37699/1‑151 (MQ=255)
                                                                                                                                               ccactaGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGt   >  1:242866/1‑151 (MQ=255)
                                                                                                                                                cactaGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTa  >  2:609145/1‑151 (MQ=255)
                                                                                                                                                cactaGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTa  >  2:239252/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCAGGTACCGGTTACGCGTACCACCAGGATCGTATGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTA  >  NC_000913/4189301‑4189595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑