Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	263,172	A→C	pseudogene (41/63 nt)	ykfN ←	pseudogene, YdiA family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	263,172	0	A	C	98.6%	263.6 / NA	73	pseudogene (41/63 nt)	ykfN	pseudogene, YdiA family
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (42/30);  minor base T (1/0);  total (43/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTAGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGT  >  NC_000913/263032‑263318                                                                                                                                             |                                                                                                                                                   cAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACCAATTCGCTCATTATg                                                                                                                                          >  1:640072/1‑151 (MQ=255)   aCGAAGATCAATCTTATCTACTGGCCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGAt                                                                                                                                        <  2:22306/151‑1 (MQ=255)   aCGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGTTAGGGCTTCGCTCACTATACTTCCTTGGCTGCAGGTTTAGTTGTACTCCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGAt                                                                                                                                        <  1:788661/151‑1 (MQ=255)   aCGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGAt                                                                                                                                        <  1:398143/151‑1 (MQ=255)   aCGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGAt                                                                                                                                        <  1:82746/151‑1 (MQ=255)   aCGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGAt                                                                                                                                        <  2:705439/151‑1 (MQ=255)   aCGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGAt                                                                                                                                        <  2:632348/151‑1 (MQ=255)           cAATCTTATCTACTGACCAAAAAGGCCGGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGGTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATCAAGCTCGAACAAATTTGCTCATTATGATCGGTTAAt                                                                                                                                >  1:693643/1‑151 (MQ=255)             aTCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATa                                                                                                                               >  2:385224/1‑150 (MQ=255)             aTCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATa                                                                                                                               >  2:627917/1‑150 (MQ=255)             aTCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATa                                                                                                                               >  2:606558/1‑150 (MQ=255)             aTCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATa                                                                                                                               >  2:445232/1‑150 (MQ=255)             aTCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATAc                                                                                                                              <  2:95534/151‑1 (MQ=255)             aTCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCACTCATTATGATCGGTTAATAc                                                                                                                              <  2:371557/151‑1 (MQ=255)             aTCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAAGTCGCTCATTATGATCGGTTAATAc                                                                                                                              <  1:393331/151‑1 (MQ=255)              tCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACt                                                                                                                             <  1:569382/151‑1 (MQ=255)               cTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACtt                                                                                                                            >  2:424043/1‑151 (MQ=255)               cTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACtt                                                                                                                            >  1:479947/1‑151 (MQ=255)               cTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACtt                                                                                                                            >  2:50681/1‑151 (MQ=255)               cTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACtt                                                                                                                            >  1:393469/1‑151 (MQ=255)               cTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACtt                                                                                                                            >  2:527967/1‑151 (MQ=255)               cTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAAGGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACtt                                                                                                                            <  2:750979/151‑1 (MQ=255)               cTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGATTAGTTGTACACCACTCCTAACTTTCATGTGTTGGCAATGCGTTCAATAAAGCTCGAACAAATTCGCTCATTATGCTCGGTTAATACtt                                                                                                                            >  1:42022/1‑151 (MQ=255)                    cTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCaa                                                                                                                         >  1:625753/1‑149 (MQ=255)                     tACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACtt                                                                                                                      <  1:741488/151‑1 (MQ=255)                         gACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTgg                                                                                                                  >  2:390258/1‑151 (MQ=255)                          aCCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGt                                                                                                                 <  1:664194/151‑1 (MQ=255)                                    ccTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGAttg                                                                                                       <  1:265165/151‑1 (MQ=255)                                      tGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGAttgtt                                                                                                     >  1:318332/1‑151 (MQ=255)                                       gATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGAttgttt                                                                                                    >  2:21334/1‑151 (MQ=255)                                       gATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGAttgttt                                                                                                    >  2:391298/1‑151 (MQ=255)                                                     cACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGAt                                                                                      <  2:740261/151‑1 (MQ=255)                                                     cACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGAt                                                                                      >  1:115788/1‑151 (MQ=255)                                                       cTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCACTGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATaa                                                                                    >  1:780275/1‑151 (MQ=255)                                                       cTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATaa                                                                                    >  2:478895/1‑151 (MQ=255)                                                       cTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATaa                                                                                    >  2:492456/1‑151 (MQ=255)                                                       cTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATaa                                                                                    >  2:319069/1‑151 (MQ=255)                                                       cTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATaa                                                                                    >  2:730976/1‑151 (MQ=255)                                                       cTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATaa                                                                                    >  2:106436/1‑151 (MQ=255)                                                       cTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATaa                                                                                    >  1:277129/1‑151 (MQ=255)                                                       cTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATaa                                                                                    >  1:769178/1‑151 (MQ=255)                                                                           gTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGtagtag                                                                 <  1:802740/150‑1 (MQ=255)                                                                           gTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGtagtag                                                                 >  1:238828/1‑150 (MQ=255)                                                                            tttAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGc                                                                <  2:15535/150‑1 (MQ=255)                                                                                gTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGgcg                                                           >  1:130029/1‑151 (MQ=255)                                                                                gTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGgcg                                                           >  2:23943/1‑151 (MQ=255)                                                                                gTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGgcg                                                           >  2:255395/1‑151 (MQ=255)                                                                                 ttGTGCACCACTCCTAACTTTAATATATTGGAAATGTGGTCAATAAAGCGCGAACAAATTCGCTCATTATGATCGGTTAATACTTCACCTTCTGGTTGCGTCATAGTGTGCCAGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGgcgc                                                          <  1:637918/151‑1 (MQ=255)                                                                                 ttGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGgc                                                            <  1:52484/149‑1 (MQ=255)                                                                                        ccacTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACg                                                    >  2:95698/1‑150 (MQ=255)                                                                                        ccacTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGc                                                   <  1:377903/151‑1 (MQ=255)                                                                                           cTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAAt                                                >  2:157634/1‑151 (MQ=255)                                                                                           cTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAAt                                                >  1:198295/1‑151 (MQ=255)                                                                                           cTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAAt                                                >  1:388326/1‑151 (MQ=255)                                                                                             ccTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAg                                              >  1:7728/1‑151 (MQ=255)                                                                                             ccTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAg                                              >  2:664113/1‑151 (MQ=255)                                                                                             ccTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCATAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAg                                              <  1:621441/151‑1 (MQ=255)                                                                                              cTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAgg                                             <  1:14523/151‑1 (MQ=255)                                                                                              cTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAgg                                             <  2:712068/151‑1 (MQ=255)                                                                                                 aaTTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTaa                                          >  1:229633/1‑151 (MQ=255)                                                                                                   tttAATGTGTTGGCGATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAAc                                        <  2:81709/151‑1 (MQ=255)                                                                                                   tttAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAAc                                        <  2:388530/151‑1 (MQ=255)                                                                                                   tttAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAAc                                        <  2:794564/151‑1 (MQ=255)                                                                                                    ttAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACa                                       <  2:626031/151‑1 (MQ=255)                                                                                                    ttAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACa                                       <  2:393678/151‑1 (MQ=255)                                                                                                                   tgtgTTCGATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAg                        <  1:415736/151‑1 (MQ=255)                                                                                                                    gtgtTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGa                       >  1:77383/1‑151 (MQ=255)                                                                                                                    gtgtTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGa                       >  2:206200/1‑151 (MQ=255)                                                                                                                    gtgtTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGa                       >  1:615390/1‑151 (MQ=255)                                                                                                                       ttCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGaaa                     <  1:763255/150‑1 (MQ=255)                                                                                                                         cAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTTCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGt                  >  1:150909/1‑151 (MQ=255)                                                                                                                         cAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGt                  >  2:323238/1‑151 (MQ=255)                                                                                                                                         aaTTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGttta  >  2:64445/1‑149 (MQ=255)                                                                                                                                             |                                                                                                                                                   CAACGAAGATCAATCTTATCTACTGACCAAAAAGGCCTGATAGGGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTAGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACGCAATAGGTAAACAAGGGAGGAAGTTCAGAAATGTAAATCGGGAAGGTTGT  >  NC_000913/263032‑263318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑