Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,914,888 T→G Q216P (CAG→CCG)  proQ ← RNA chaperone, putative ProP translation regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,914,8880TG98.4% 220.5 / NA 64Q216P (CAG→CCG) proQRNA chaperone, putative ProP translation regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (39/24);  minor base C (0/1);  total (39/25)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTT  >  NC_000913/1914742‑1915026
                                                                                                                                                  |                                                                                                                                          
atCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGgga                                                                                                                                         >  2:524952/1‑150 (MQ=255)
atCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGgga                                                                                                                                         >  1:543202/1‑150 (MQ=255)
  cttctACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGggacg                                                                                                                                       >  2:782208/1‑150 (MQ=255)
      tACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGggacgcggac                                                                                                                                  <  2:423856/151‑1 (MQ=255)
          gCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCg                                                                                                                              >  2:65148/1‑151 (MQ=255)
          gCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCg                                                                                                                              >  2:598149/1‑151 (MQ=255)
               ggTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCttt                                                                                                                         >  1:589336/1‑151 (MQ=255)
                  cTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGATGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGt                                                                                                                      <  2:679813/151‑1 (MQ=255)
                       cTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGAttt                                                                                                                 >  2:590888/1‑151 (MQ=255)
                         gCTATTGCAAGCAGGCCAGCTAACGCGGTATGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCCGACGCCGTCTTTGGTGATTTc                                                                                                                >  1:669264/1‑150 (MQ=255)
                         gCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGAttt                                                                                                                 >  2:156683/1‑149 (MQ=255)
                         gCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGAttt                                                                                                                 >  1:106281/1‑149 (MQ=255)
                            aTTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAAt                                                                                                            >  1:489640/1‑151 (MQ=255)
                             ttGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTGCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATa                                                                                                           <  1:505912/151‑1 (MQ=255)
                              tGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATAc                                                                                                          <  1:123532/151‑1 (MQ=255)
                              tGCAAGCAGGCCAGCAAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATAc                                                                                                          <  1:618904/151‑1 (MQ=255)
                               gCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCAGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACg                                                                                                         >  2:559742/1‑151 (MQ=255)
                                cAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACgg                                                                                                        <  2:498203/151‑1 (MQ=255)
                                 aaGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGt                                                                                                       <  2:698377/151‑1 (MQ=255)
                                      ggCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGcat                                                                                                  >  2:597596/1‑151 (MQ=255)
                                        ccAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGcatc                                                                                                 <  1:670451/150‑1 (MQ=255)
                                           gCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGcatccat                                                                                              >  1:687518/1‑150 (MQ=255)
                                            cTAACGCGTTAAGCCTAAATAACATGTTCATGCCTCGCCCGGCCGCCGTTTCAGCACACCAGCCTCCCTTCGCGCCCAATCAAAGACATACCCGAAGTCATCCCGACGCGGATGCCGTCTTTGGTGATTTCTAATGAGGTGGcatccat                                                                                              <  1:692551/149‑1 (MQ=255)
                                            cTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATcg                                                                                            <  2:722477/151‑1 (MQ=255)
                                               aCGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGt                                                                                         <  2:466977/151‑1 (MQ=255)
                                                        gCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCg                                                                                >  2:746710/1‑151 (MQ=255)
                                                                aaCATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCAcc                                                                        <  2:297114/151‑1 (MQ=255)
                                                                   aTGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTc                                                                     <  2:766303/151‑1 (MQ=255)
                                                                    tGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCa                                                                    >  2:253382/1‑151 (MQ=255)
                                                                     gTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGcatccat                                                                                              >  2:573743/1‑124 (MQ=255)
                                                                     gTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGcatccat                                                                                              <  1:573480/124‑1 (MQ=255)
                                                                        cATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGc                                                                <  2:632039/151‑1 (MQ=255)
                                                                          tGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCtt                                                              >  2:630309/1‑151 (MQ=255)
                                                                           gCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTg                                                             >  2:702147/1‑151 (MQ=255)
                                                                                   cGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAg                                                     <  1:361558/151‑1 (MQ=255)
                                                                                    ggCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGt                                                    >  2:541155/1‑151 (MQ=255)
                                                                                         ccGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCagag                                               >  1:167569/1‑151 (MQ=255)
                                                                                         ccGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCagag                                               >  2:681659/1‑151 (MQ=255)
                                                                                         ccGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCagag                                               >  1:242977/1‑151 (MQ=255)
                                                                                                  aaCACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATCCGGTGGCATCCATCGCGTTTTGACCCGGTTTCACCTTCATGGCTTGTCCGACAGTCAgcgccgcta                                         <  1:195544/148‑6 (MQ=255)
                                                                                                  aaCACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGaaa                                         <  2:242567/148‑1 (MQ=255)
                                                                                                  aaCACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGGTTGGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATg                                       <  1:205517/150‑1 (MQ=255)
                                                                                                    cacCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCa                                    >  2:512503/1‑151 (MQ=255)
                                                                                                    cacCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCa                                    >  1:319169/1‑151 (MQ=255)
                                                                                                      ccAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGa                                  >  1:675081/1‑151 (MQ=255)
                                                                                                      ccAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGa                                  >  2:71553/1‑151 (MQ=255)
                                                                                                          gtgtTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAAc                               <  2:543454/150‑1 (MQ=255)
                                                                                                                    cgcACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGc                     <  2:617288/150‑1 (MQ=255)
                                                                                                                    cgcACAATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGc                     <  1:370959/150‑1 (MQ=255)
                                                                                                                    cgcACAATCAAAGACACACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGc                     <  1:127912/150‑1 (MQ=255)
                                                                                                                      cacaATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTg                   <  2:124262/150‑1 (MQ=255)
                                                                                                                        caATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGttc                <  2:799404/151‑1 (MQ=255)
                                                                                                                        caATCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGtt                 >  2:642246/1‑150 (MQ=255)
                                                                                                                          aTCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGttctt              >  2:665274/1‑151 (MQ=255)
                                                                                                                          aTCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGttctt              >  2:636320/1‑151 (MQ=255)
                                                                                                                          aTCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGttctt              >  1:557872/1‑151 (MQ=255)
                                                                                                                          aTCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGttctt              >  1:727997/1‑151 (MQ=255)
                                                                                                                          aTCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGttctt              >  2:104183/1‑151 (MQ=255)
                                                                                                                          aTCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGGTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGttctt              >  1:747012/1‑151 (MQ=255)
                                                                                                                          aTCAAAGACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGGGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGttctt              >  1:300006/1‑151 (MQ=255)
                                                                                                                                gACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAg        >  2:668007/1‑151 (MQ=255)
                                                                                                                                gACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGCGTGCTGTTCTTCGCGAg        >  2:196748/1‑151 (MQ=255)
                                                                                                                                gACATACCCGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGGGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAg        >  2:237442/1‑151 (MQ=255)
                                                                                                                                      cccGAATTCAGCGGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCtt  >  2:60033/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                          
ATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGCCTAAAAAACATGTTCATGCCTGGCCCGGCCTCCGTTTCAGAACACCAGGTGTTCTGCGCGCACAATCAAAGACATACCCGAATTCAGCTGGACGCGGACGCCGTCTTTGGTGATTTCTAATACGGTGGCATCCATCGCGTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTT  >  NC_000913/1914742‑1915026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑