Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,338,928 | C→A | L107F (TTG→TTT) | mlaE ← | ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,338,928 | 0 | C | A | 98.3% | 226.8 / ‑6.5 | 58 | L107F (TTG→TTT) | mlaE | ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein |
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (19/38); minor base G (1/0); total (20/38) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.45e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTGGTAATGAAATAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAACACGCCAGAAACCACAAT > NC_000913/3338780‑3339074 | gTGGTAATGAAATAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCaaaaaa > 2:140691/1‑151 (MQ=255) gAAATAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCgg > 2:777413/1‑151 (MQ=255) aaaTAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCgg > 2:502643/1‑150 (MQ=255) aaaTAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCgg > 1:173602/1‑150 (MQ=255) ccccAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAacca > 2:725187/1‑150 (MQ=255) aaaCGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGtt < 2:560797/151‑1 (MQ=255) ggAGAAATACCCCGACGCATGGGTTCCCCAGCCATCGTCTACATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGACCCAGCACGACCGGCAAAAAACAACGCGGCAACCACCGGCACCAGTTCACGc < 1:167049/150‑1 (MQ=255) ggAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGc < 2:387088/150‑1 (MQ=255) agaAATAGCCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGGTGCACTGTAGCGCGCGTCAGGCCGTTTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAAGCGGCAACCGCCGGCACGAGTTCACGct < 1:32375/149‑2 (MQ=255) gaAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAACACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTa > 2:372358/1‑151 (MQ=255) aaaTAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAg > 1:511951/1‑151 (MQ=255) tAACCCGACTCATCGTATCTACCGCCATCATCTCCATTCTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCCCGCCCGGCAAAAAACAACGCGGCAACCGCCGGCCCCAGTTCACGCAGTAGCGa < 1:79499/151‑1 (MQ=255) ccGACGCAGCGGACCCACCGCCATCAACTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAAc < 2:393189/151‑1 (MQ=255) aCGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGc < 2:676240/150‑1 (MQ=255) gCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAg > 1:31342/1‑151 (MQ=255) cGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAg < 2:31345/150‑1 (MQ=255) tCCACCGCCATCATCTCCATACTGGAGAGGTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAc < 1:29951/151‑1 (MQ=255) ccccGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCACCCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCAACAGCATAccc < 1:6455/149‑1 (MQ=255) ccATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGAc < 2:266895/150‑1 (MQ=255) ccATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGAc < 2:482427/150‑1 (MQ=255) ccATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGAc < 2:741031/150‑1 (MQ=255) tctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGttt > 2:465978/1‑150 (MQ=255) tctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGttt > 1:424103/1‑150 (MQ=255) tctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGttt > 2:486749/1‑150 (MQ=255) ncATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCg > 2:721041/2‑150 (MQ=255) ccATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAGAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGCAGCGATAACGCCACAAGCATACCCAGACTGGTTTccc > 1:443037/1‑149 (MQ=255) ccATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGc < 1:802588/151‑1 (MQ=255) agagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAg < 1:690072/150‑1 (MQ=255) agagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAg < 1:404687/150‑1 (MQ=255) gagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGCAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTg > 1:101683/1‑151 (MQ=255) agTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTATCGCCGAGCCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCGCTATAAGt < 2:507773/149‑1 (MQ=255) agTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGt < 2:383304/149‑1 (MQ=255) agTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTg < 1:86175/150‑1 (MQ=255) agTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTg < 2:33970/150‑1 (MQ=255) ttGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTgg < 2:552976/149‑1 (MQ=255) gCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAg > 2:548488/1‑151 (MQ=255) gCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAg > 2:5018/1‑151 (MQ=255) ctGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGaan < 2:139397/151‑2 (MQ=255) ctGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGaa > 1:769492/1‑150 (MQ=255) gTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCa > 1:441396/1‑151 (MQ=255) cgcgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGAt < 2:431002/150‑1 (MQ=255) cgcgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGAt < 1:707141/150‑1 (MQ=255) cATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAAc > 2:664527/1‑149 (MQ=255) cTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGGAGCCCCAacac < 1:368388/151‑1 (MQ=255) cTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAacac < 1:597133/151‑1 (MQ=255) cTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAacac < 1:434153/151‑1 (MQ=255) cTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAacac < 2:262227/151‑1 (MQ=255) cTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAacac < 2:209078/151‑1 (MQ=255) cTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGATCACGCAGTAGCGATAACACCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAacac < 1:146121/151‑1 (MQ=255) ggTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATa < 2:225047/74‑1 (MQ=255) ggTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATa > 1:224987/1‑74 (MQ=255) cgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATg < 2:117321/151‑1 (MQ=255) aaCCAGCACGCCCGGCACAAACCAACGCGGCAACCGCCGTGTCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTAGTTCCCGCGCTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATCCCGATGAacac < 1:329936/151‑1 (MQ=255) aaCCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAaca > 1:34421/1‑150 (MQ=255) cACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAACACGCCAg < 2:80445/150‑1 (MQ=255) cGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAACACGCCAGaaa < 2:666373/151‑1 (MQ=255) cccGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCGCCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAACACGCCAGAAAcc < 1:412116/151‑1 (MQ=255) cAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAACACGCCAGAAACCACaat < 2:213664/151‑1 (MQ=255) cAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAACACGCCAGAAACCACaa < 1:113847/150‑1 (MQ=255) | GTGGTAATGAAATAACCCCAGCCCAGAAACGGGGAGAAATAACCCGACGCAGCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAGATAACCTTGCAGCCCCAACACCATTCCGATGAACACGCCAGAAACCACAAT > NC_000913/3338780‑3339074 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |