Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT54.4% 23.5 / 147.6 125S62F (TCT→TTT) bglJbgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (13/44);  new base T (68/0);  total (81/44)
Fisher's exact test for biased strand distribution p-value = 2.00e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.36e-13

Only 100 of 140 total aligned reads displayed.

CTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAC  >  NC_000913/4604196‑4604492
                                                                                                                                                     |                                                                                                                                                      
cTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TTCTtttttttt                                                                                                                                                      >  1:591741/1‑151 (MQ=255)
cTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATCCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATtttttttt                                                                                                                                                      >  2:429098/1‑151 (MQ=255)
  cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATtttttctttt                                                                                                                                                    >  1:1698685/1‑151 (MQ=255)
    tAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑GCCttttttttttttt                                                                                                                                                  >  1:1471847/1‑150 (MQ=255)
      gAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCAttttttttttttttt                                                                                                                                                >  1:620923/1‑148 (MQ=255)
              cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATgaga                                                                                                                                        <  2:897682/151‑1 (MQ=255)
                tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAGGCTCCATACCTATGCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAg                                                                                                                                      <  1:1440296/151‑1 (MQ=255)
                tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAg                                                                                                                                      <  1:1331853/151‑1 (MQ=255)
                 cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTCTTCCATgcggagg                                                                                                                                     >  1:466105/1‑145 (MQ=255)
                    gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTTTTCCATGAGAAGTGAg                                                                                                                                  >  2:184045/1‑151 (MQ=255)
                    gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑GCATTTTTTTTTTTTTTGTCTTGTGAAGGGAg                                                                                                                                  >  1:1199020/1‑151 (MQ=255)
                       tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc                                                                                                                               <  2:622261/151‑1 (MQ=255)
                       tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑CCATTTTTTTTTTTTTTGCCCTGAGAAGTGAggg                                                                                                                                >  1:1509108/1‑148 (MQ=255)
                         gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTTCCCTGAGAAGTGAgcgcgg                                                                                                                             >  1:283165/1‑149 (MQ=255)
                         gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTTCCATTAGAAGGGAGGGCag                                                                                                                             >  2:434431/1‑151 (MQ=255)
                         gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTTCCATGGGAAATGAGCGCag                                                                                                                             >  2:1765297/1‑151 (MQ=255)
                         gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATtttttc                                                                                                                                                        <  2:963733/124‑1 (MQ=255)
                         gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCATGAGAAGAGAGCGCag                                                                                                                             >  1:1429464/1‑151 (MQ=255)
                         gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑ACATTTTTTTTTTTTTTGTCATTAGGAGAGAGCGCag                                                                                                                             >  1:1565182/1‑151 (MQ=255)
                               gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCTTTTTTTTTTTTTTTTCCATTAGGAGTGAGCGGAGgggggg                                                                                                                       >  2:1696231/1‑151 (MQ=255)
                               gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCATTAGAAGGGAGCGCAGgggggg                                                                                                                       >  2:1308141/1‑151 (MQ=255)
                               gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTCTGCCATGAGAAGGGAGCGCAGAGAggg                                                                                                                       >  1:140269/1‑151 (MQ=255)
                               gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑ACATTTTTTCTTTTTCTGCCAAGAGAAGGGAGGGCGGAGAggg                                                                                                                       >  2:1439148/1‑151 (MQ=255)
                                 aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCCGAGAggggt                                                                                                                     >  2:1337639/1‑149 (MQ=255)
                                       agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATc                                                                                                                 <  2:345142/149‑1 (MQ=255)
                                       agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATc                                                                                                                 <  1:800606/149‑1 (MQ=255)
                                       agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCt                                                                                                                <  1:79972/150‑1 (MQ=255)
                                       agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCt                                                                                                                <  2:735901/150‑1 (MQ=255)
                                           tATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCc‑t                                                                                                          <  1:381201/151‑1 (MQ=255)
                                            aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTTCATGGGGAGGGAGCGCCGAGGGGGGGTATTTTTCc‑tt                                                                                                         >  2:496077/1‑150 (MQ=255)
                                                  aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCc‑tgact                                                                                                      <  1:922245/148‑1 (MQ=255)
                                                     gTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCTTTTTTTTTTTTTTTTTCCAGAGAAGTGGGGGCCGAGAGGGGGTATCTTGGC‑TTAATGaa                                                                                                   >  2:279671/1‑148 (MQ=255)
                                                           gggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTCTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTCTTTTTCC‑TGTCTGAACTGGCGAtt                                                                                          >  1:1559795/1‑151 (MQ=255)
                                                              tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAg                                                                                      <  1:631614/151‑1 (MQ=255)
                                                              tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAg                                                                                      <  2:1237310/151‑1 (MQ=255)
                                                              tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATCATCTTGCC‑TGACTGAACTGGCGATT‑AAg                                                                                      <  1:1244672/151‑1 (MQ=255)
                                                                 cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTTCCCTGAGAAGTGGGGGCCGGGAGGGGTTTTTTTTCC‑TGACTTGACTTGCGGTT‑TAGttt                                                                                   >  2:474781/1‑151 (MQ=255)
                                                                 cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTGCCCTTGGAAGGGAGCGCAGAGGGGGGTTTTTTTTCC‑TTACTGAAATGGGGGTT‑AAGttt                                                                                   >  2:250658/1‑151 (MQ=255)
                                                                 cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTCTTCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCC‑TGACTGAACTGGCGATT‑TAGttt                                                                                   >  1:495358/1‑151 (MQ=255)
                                                                 cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGCGAGGGATTATCTTGCC‑TGACTGAACCGGCGATT‑AAGttt                                                                                   >  1:374227/1‑151 (MQ=255)
                                                                 cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTTTGCCCTGAGGAGTGGGGGGCGAGAGGGGTTATCTTTCC‑TTATTGAACTGGGGATT‑TAGgtt                                                                                   >  1:645994/1‑151 (MQ=255)
                                                                 cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGcc                                                                                                            <  2:1540821/128‑1 (MQ=255)
                                                                 cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑GCATTTTTTTTTTTTCTGCCATGAGAAGTGGGCGCAGGGAGGGGTTATCTTTCC‑TGACTGAACTGGCGGTT‑TAGttt                                                                                   >  1:1210110/1‑151 (MQ=255)
                                                                   cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCCGAGAGGGGTTTTTTTGCC‑TGACTGGAATGGCGATT‑AAGtttt                                                                                  >  1:1093175/1‑149 (MQ=255)
                                                                   cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCCGAGAGGGGTTTTTTTGCC‑TGACTGAACTGGGGGTT‑TAGtttt                                                                                  >  2:791786/1‑149 (MQ=255)
                                                                   cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCCTGAGAAGAGGGGGCAGGGGGGGGGTTTTTTGCC‑TTACTTTACTgggggtttaggttt                                                                                   >  1:832733/1‑138 (MQ=255)
                                                                   cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑GCATTTTTTTTTTTTTTGCCCTGAGGAGTGAGCGCCGAGGGGGATTTTTTTGCC‑CGAATGGAATGGCGGTT‑TAGATTc                                                                                  >  1:829831/1‑150 (MQ=255)
                                                                          gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCATGAGGAGGGAGCCCCGGGAGGGGTTATCTTGTC‑TGACTGAACTGGCGGTT‑AAGATTTCGCGttc                                                                           >  1:1544960/1‑148 (MQ=255)
                                                                          gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCCGAGAGGGGTTATCTTGCC‑TGACTGAAATGGCGATT‑AAGTTTCCGCGTAc                                                                           >  2:1543334/1‑150 (MQ=255)
                                                                           cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCTTTTTTTTTTTTTCTGCCCTGAGAAGGGAGGGCCGAGAGGGGTTTTCTTTCC‑TTACTGAACTGGCGGTT‑AAGTTTCCGCGTAcc                                                                          >  2:31739/1‑150 (MQ=255)
                                                                           cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTTCCCTGAGAAGTGGGGGCCGAGAGGGGTTTTCTTGCC‑TTACTTAACTGGGGGTT‑TAGTTTTCCCGTAcc                                                                          >  2:1656994/1‑150 (MQ=255)
                                                                           cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCCGAGAGGGGTTTTTTTGCC‑TGNNTGAACTGGCGATT‑AAGGTTTCGCGTAcc                                                                          >  1:1600967/1‑150 (MQ=255)
                                                                           cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGGCGAGAGGGGTTATCTTTCC‑CGGATTAACTGGGGAGT‑AAGTTTTCGCGTAcc                                                                          >  1:1408663/1‑150 (MQ=255)
                                                                           cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGGGGGAATATCTTGCC‑CGACTGAACTGGCGGTT‑TAGGTTCCGCGtcac                                                                          >  1:762067/1‑147 (MQ=255)
                                                                           cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTCTTCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCC‑TGACTGAAACGGCGATT‑TAGTTTCCCCGTAcc                                                                          >  1:282686/1‑150 (MQ=255)
                                                                           cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑GCATTTTTTTTTTTTTTGCCATTAGGAGTGAGCGCAGAGAGGGGTTATCTTGCC‑TGACTGAACTGGCGATT‑TAGGTTTCGCGGAcc                                                                          >  1:843210/1‑150 (MQ=255)
                                                                            tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTCTTTTTTTTTTTTTTTGCC‑CGAGAAGGGAGGGCCGAGAGGGGTTTTTTTTCCTTGATTG‑ACTGGCGATT‑TAGGTTTCGCGGACCCg                                                                        >  2:1401978/1‑151 (MQ=255)
                                                                                tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTTTCCTGAGAAGGGAGCGCCGAGGGGGGTTTTTTTTCC‑TTACTGAAACGGCGGTT‑TAGGTTTCGCGGAACCGGCgg                                                                    >  1:1773692/1‑150 (MQ=255)
                                                                                tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTGCCCTGAGAAGGGGGGGCAGGGAGGGAGTATCTTTCC‑TGACTGAAACGGCGATT‑AAGTTTTCGCGGACCCcgcgg                                                                    >  1:753567/1‑150 (MQ=255)
                                                                                tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCCTTAGAAGGGAGGGCAGAGAGGGGTTATCTTTCC‑TGACTGAAATGGCGGTT‑AAGTTTCCGCGTACCCCGCGt                                                                    >  2:141005/1‑151 (MQ=255)
                                                                                tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCC‑TGACTGAACTGGCGGTT‑AAGTTTTCGCGTAACCGGCGt                                                                    >  1:950600/1‑151 (MQ=255)
                                                                                tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGCGCAGAGAGGGGTTATCTTTCC‑TGACTGAACTGGCGGTT‑AAGTTTCCCCGTACCCGGCGt                                                                    >  2:1605005/1‑151 (MQ=255)
                                                                                tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTCTGCCCTTGGAAGGGAGGGCAGAGGGGGGTTATCTTTCC‑TTGCTGAACCGGCGATT‑TAGTTTTCGCGGACCCGGCgg                                                                    >  2:1232020/1‑150 (MQ=255)
                                                                                 aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTTTTTTTTTGCCCTGAGAAGGGAGGGCCGAGGGGGGTTATCTTTCC‑TGACTGAAATGGCGGTT‑AAGGTTTCCCGGACCCGGCggt                                                                   >  2:615651/1‑149 (MQ=255)
                                                                                 aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCCTTTTTTCTTTTTCTTCCCTGAGAAGTGGGCGCAGAGGGGGGTTATCTTGCC‑TGACTGAAATGGCGATT‑AAGTTTTCGCGGACCCGGCGtt                                                                   >  2:268712/1‑151 (MQ=255)
                                                                                 aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCATGGGAAGAGGGCGCCGAGGGGGGTTTTTTTGCC‑TGGCTGAAATGGGGATT‑TAGGTTTCGCGGACCCGGCggt                                                                   >  2:1261054/1‑149 (MQ=255)
                                                                                 aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCCTGAGAAGGGGGCGCAGAGGGGGGTTATCTTGCC‑TTACTTGACCTGCGGTT‑AAGTTTTCCCGGACCCGGCggt                                                                   >  2:1670117/1‑149 (MQ=255)
                                                                                     ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGACTATCTTGCC‑TTCTTTAACTGGCGCTT‑TAGGTTTCCCGGTCCCGGCGGTTTGt                                                               >  1:72146/1‑151 (MQ=255)
                                                                                     ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCATGGGAAGGGAGCGCAAAGAGGGGGTTTCTTGGC‑TGAATGAAAAGGGGATT‑AAGTTTCCGCGGAACCCGCGgtttgg                                                               >  2:374458/1‑148 (MQ=255)
                                                                                     ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTTTTCCCTGAGAAGTGAGGGCAGAGAGGGGTTTTCTTTCC‑TGACTGAACTGGCGATT‑TAGTTTCCCCGGACCCCGCGGTTAGt                                                               >  1:1754023/1‑151 (MQ=255)
                                                                                      taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGtt                                                              <  1:1687110/151‑1 (MQ=255)
                                                                                          aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑GCCTTTTTTTTTTTTCTTCCCAGAGAAGGGGGCGCCGGGAGGGGTTTTTTTTGC‑TTGCTTGACTGGGGGTT‑AAGGTTCCGCGTACCCGGGGttttggttttg                                                          >  2:804002/1‑143 (MQ=255)
                                                                                           gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCATGGGAAGGGAGCGCAGAGAGGGGTTTTCTTGCC‑TGACTGAACTGGCGGTT‑TAGTTTCCGCGGACCCGGCGTTTAGTTTTTg                                                          >  2:1487699/1‑150 (MQ=255)
                                                                                                gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                                     <  2:1070352/150‑1 (MQ=255)
                                                                                                 aGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTCTTCCCTGAGAAGGGGGCGCAGGGGGGGGTTATCTTTCC‑TGACTTAACTTGCGGTT‑AAGTTTCCTCGGTCCCCGGGTTTTGTTTTTGTggggtg                                                   >  2:70713/1‑147 (MQ=255)
                                                                                                  gTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑ACCTTTTTTTTTTTTTTCCCATGTAAAATGTGCGCCGGGGGGGGTTTTTTTTTC‑CGGCTGCACTGGGGAAT‑AAATTTCTGCCGGCCCGGCGgttttgttttgtggctg                                                    >  1:1537097/1‑135 (MQ=255)
                                                                                                    cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTCTTCCCTGAGAAGGGAGGGCCGAGAGGGGTTATCTTGCC‑TGGCTGAAATGGGGATT‑AAGGTTTCCCGGACCCGGGGGTTTGTTTTTTCGggtggtga                                                >  2:851808/1‑151 (MQ=255)
                                                                                                      tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCGTCTTTTCTTTTTTTGCCCCTAGAAAAGGGTGCAGAGGGGGGTTATTTTTCC‑TGACTGGACTGGTGGTT‑GTGTTTCCCCGCAACCGGCGGTTCGTTGTTGccggtgttggtg                                              >  1:1370408/1‑140 (MQ=255)
                                                                                                       ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGatat                                             <  2:1728859/151‑1 (MQ=255)
                                                                                                       ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGatat                                             <  2:843153/151‑1 (MQ=255)
                                                                                                         cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑GCATTTTTTTTTTTTTTGCCATGAGAAGGGAGGGCAGGGAGGGGTTATCTTGCC‑TTACTGAAATGGCGGTT‑AAGTTTTCCCGTACCCGGCGTTTTGTTTTTTCGGGTGATTATAttt                                           >  2:84526/1‑150 (MQ=255)
                                                                                                            gATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGa                                          <  2:897865/149‑1 (MQ=255)
                                                                                                             aTGCCATGTCGCGGATCTCGTTTGCGGCGG‑TTATTTTTTTTTTTTTTGCCATGGGAAGGGAGGGCGGGGGGGGGTTTTCTTGCC‑TGACTGAAATGGGGGTT‑AAATTTCCGCGGACCCGGGGGTTTGGTATTGGGGGTGGTGGAATTTAAg                                        >  2:890950/1‑150 (MQ=255)
                                                                                                             aTGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGGGGGGGGGTTTTTTGCC‑TGGCTGAACTGGGGATT‑AAATTTCCGCGTACCCGGCGGTTTGTTATTGCGGGTGATGATAtttgagg                                       >  1:1159384/1‑146 (MQ=255)
                                                                                                              tGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCt                                      <  2:627375/151‑1 (MQ=255)
                                                                                                              tGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCt                                      <  2:244724/151‑1 (MQ=255)
                                                                                                               gCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTTTTTTTTTGCCACTAGAAGGGGGCGCAGAGAGGGGTTATCTTGCC‑TGACTGAAATGGGGATTAAAGTTTCC‑CGTACCCCGGGTTTTTTTTTTTGGGGTGATGATATTTTAGCt                                      >  2:273143/1‑150 (MQ=255)
                                                                                                                   tGTCGCGGATCTCGTTTGCGGCGG‑GCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATTTTTCC‑TTACTGAACTGGCGATT‑TAGGTTTCGCGGACCCCGGGTTTTGGTTTTGCGGGTGATGATATTTGAACTCgggg                                 >  1:331886/1‑149 (MQ=255)
                                                                                                                        cgGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAtt                             <  1:553610/150‑1 (MQ=255)
                                                                                                                        cgGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCACGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAtt                             <  1:1229333/150‑1 (MQ=255)
                                                                                                                          gATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTgg                           <  2:1199431/150‑1 (MQ=255)
                                                                                                                          gATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTgg                           <  1:7429/150‑1 (MQ=255)
                                                                                                                                 tttGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGt                   <  1:1143683/151‑1 (MQ=255)
                                                                                                                                     cggcgg‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGcc               <  2:1159934/151‑1 (MQ=255)
                                                                                                                                       gcgg‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCa              <  1:1231996/150‑1 (MQ=255)
                                                                                                                                            tCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAc          <  2:9284/150‑1 (MQ=255)
                                                                                                                                              aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGc       <  2:18493/151‑1 (MQ=255)
                                                                                                                                                   ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc  <  1:730635/151‑1 (MQ=255)
                                                                                                                                                   ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc  <  1:474614/151‑1 (MQ=255)
                                                                                                                                                   ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc  <  2:1281139/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑TCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCC‑TGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAC  >  NC_000913/4604196‑4604492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑