Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,622,2500AG48.0% 105.6 / 57.1 103E161G (GAG→GGG) yjjJputative protein kinase
Reads supporting (aligned to +/- strand):  ref base A (10/42);  new base G (48/0);  total (61/42)
Fisher's exact test for biased strand distribution p-value = 6.96e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

ACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGAGAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCT  >  NC_000913/4622101‑4622399
                                                                                                                                                      |                                                                                                                                                       
aCCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAGGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCggggag                                                                                                                                                        <  2:538749/151‑1 (MQ=255)
aCCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCggggag                                                                                                                                                        <  2:493552/151‑1 (MQ=255)
      gggCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGGT‑GGGTGGTCGGGGGGGGGGAt                                                                                                                                                  >  2:1649954/1‑151 (MQ=255)
        gCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGGTTGGTCgggggggggatta                                                                                                                                                 >  1:698640/1‑148 (MQ=255)
        gCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGGCGGGGGGGGGGATTa                                                                                                                                                >  2:960329/1‑151 (MQ=255)
        gCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGGTGGTCGGGGGGGGGGATTa                                                                                                                                                >  2:46698/1‑151 (MQ=255)
        gCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGGGAATATACTGGCGGTT‑GGTTGGTCGGGGGGGGGGATTa                                                                                                                                                >  2:11641/1‑151 (MQ=255)
          tttttAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATc                                                                                                                                              <  2:441898/151‑1 (MQ=255)
          tttttAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATc                                                                                                                                              <  2:920738/151‑1 (MQ=255)
          tttttAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGGTGGGGGGGGGGATTAt                                                                                                                                               >  2:44592/1‑150 (MQ=255)
            tttAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAg                                                                                                                                            <  1:858921/151‑1 (MQ=255)
                 ggCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGa                                                                                                                                         <  1:1234652/149‑1 (MQ=255)
                     cgcgTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGAtt                                                                                                                                   <  1:516085/151‑1 (MQ=255)
                           gggCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGc                                                                                                                              <  1:240941/150‑1 (MQ=255)
                           gggCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCGACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGGTGGGCGGGGAGGGGAATTATCAGCGATGGATTACTGca                                                                                                                             >  1:1226677/1‑151 (MQ=255)
                                ggAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGGCCGGGCGGGGAATTATCATCGATGGTTTACCGCacaaca                                                                                                                        >  1:340333/1‑151 (MQ=255)
                                 gAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCacaac                                                                                                                         <  2:607760/149‑1 (MQ=255)
                                 gAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCacaac                                                                                                                         <  2:1119193/149‑1 (MQ=255)
                                 gAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCacaaa                                                                                                                         <  2:29748/149‑2 (MQ=255)
                                  aaGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAacac                                                                                                                       <  2:1012558/150‑1 (MQ=255)
                                  aaGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAacac                                                                                                                       <  1:1589445/150‑1 (MQ=255)
                                  aaGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAacac                                                                                                                       <  1:417647/150‑1 (MQ=255)
                                     ttAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACAcccc                                                                                                                    <  2:960148/150‑2 (MQ=255)
                                     ttAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACAccc                                                                                                                     <  1:156831/149‑1 (MQ=255)
                                     ttAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACAccc                                                                                                                     <  2:1151345/149‑1 (MQ=255)
                                     ttAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACAccc                                                                                                                     <  2:12891/149‑1 (MQ=255)
                                     ttAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACAccc                                                                                                                     <  1:1136314/149‑1 (MQ=255)
                                     ttAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACAccc                                                                                                                     <  1:605028/149‑1 (MQ=255)
                                         ccGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑gg                                                                                                               <  2:736869/150‑1 (MQ=255)
                                              cAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTc                                                                                                         <  1:561457/151‑1 (MQ=255)
                                              cAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGGTGGTCGGGGGGGGGGATTATCAGCGATGGGTTACTGCACAACACCCCGC‑GGGAAtt                                                                                                          >  2:266273/1‑150 (MQ=255)
                                                aCTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGGGGGGGATTTTCAGCGGCGGGTTACTGCACCACCCCCTGC‑GGGAATTc                                                                                                         >  2:161787/1‑149 (MQ=255)
                                                aCTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGGTGGTTGGGGGGGGGAATTATCAGCGATGGGTTTCTTCACAACACCCCGCGGGAAAtt                                                                                                          >  2:797235/1‑149 (MQ=255)
                                                aCTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGGTGGTCGGGGGGGGGGATTTTTAGGGATGGGTTTCTGCACAAAACCCCGCGGGAAAtt                                                                                                          >  2:1702898/1‑149 (MQ=255)
                                                aCTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGGTGGTCGGGGGGGGGGAATATCAGCGAAGGATTACTTCACAACACCCTGC‑GGaaaatt                                                                                                         >  2:1533342/1‑146 (MQ=255)
                                                aCTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGGTGGGCGGGGAGGGGGATTATCAAGGATGGATTTCTCGACAACACCCTGC‑GGAAATTc                                                                                                         >  1:956621/1‑149 (MQ=255)
                                                 cTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCctc                                                                                                      <  2:843620/151‑1 (MQ=255)
                                                   gAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGGCCGGGGGGGGAAATATCAGCGATGGGTTTCTGCACAACACCCCGC‑GGGAATTCct                                                                                                       >  2:396136/1‑148 (MQ=255)
                                                       ctgactgaTGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑Tc                                                                                               <  2:79459/151‑1 (MQ=255)
                                                         gactgaTGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGGCGGGGGGGGGGATTATCAGCGATGGGTTACTGCCCAACACCCCGC‑GGGAAATTCTCTgggacaa                                                                                              >  2:1418227/1‑146 (MQ=255)
                                                         gactgaTGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGGT‑GGGTGGTCGGGGAGGGGGATTATCAGCGATGGATTACTGCCCAACACCCCGC‑GGGAATTTCTCTGGG‑TCaa                                                                                             >  1:621128/1‑151 (MQ=255)
                                                            tgatgaTATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCaaaaa                                                                                          <  1:578007/151‑1 (MQ=255)
                                                             gatgatATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑TGGTGGGCGGGGAGGGGGATTATCAGGGATGGGTTAATGCAAAAAAACCCGC‑GGAAAATTCTTTGGA‑TCaaaaaa                                                                                         >  1:251871/1‑150 (MQ=255)
                                                             gatgatATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGGGGGGGATTTTCAGCGATGGATTTCTGCACCACACCCCGC‑GGGAATTCCTCTGGA‑TCcaaaaa                                                                                         >  1:927311/1‑150 (MQ=255)
                                                             gatgatATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGGGGGGGAATTTCAGCGATGGGTTTCTGCACAACACCCCGC‑GGGAATTCCTCTGGG‑TCaaaaaa                                                                                         >  2:103151/1‑150 (MQ=255)
                                                             gatgatATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGGGGGGGAATATCAGCGGTGGGTTACTGCACAACACCCTGC‑GGGAATTCCTCTGGG‑TCaaaaaa                                                                                         >  2:282542/1‑150 (MQ=255)
                                                             gatgatATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGGT‑TGGTGGTCGGGGGGGGGAATTATCAGCGATGGGTTACTGCCCAAAACCCTGC‑GGGAATTTCTCTGGA‑TCaaaaaa                                                                                         >  2:1291707/1‑150 (MQ=255)
                                                             gatgatATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGGT‑GGTTGGGCGGGGGGGGGGATTATCAGCGAGGGATTACTGGACAACACCCCGC‑GGAAATTTCTCTTGA‑TCcaaaaa                                                                                         >  1:1505076/1‑150 (MQ=255)
                                                              atgatATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAAc                                                                                         <  1:1229618/150‑1 (MQ=255)
                                                              atgatATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGGCGGGGGGGGGGATTATTAGCGATGGGTTACTGCACAAAACCCCGC‑GGAAATTTCTTTTGA‑TCaaaaaat                                                                                        >  1:1365761/1‑149 (MQ=255)
                                                              atgatATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGGCGGGGGGGGGAATTATCAGCGATGGATTACTGCACAACACCCCGC‑GGGAATTCCTCTGGG‑TCaaaaaat                                                                                        >  2:478364/1‑149 (MQ=255)
                                                               tgatATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGGGGGGAATTATCAGCGATGGATTACTGCACAAAACCCCGC‑GGGAAATTCTCCGGA‑TCaaaaaat                                                                                        >  2:4851/1‑148 (MQ=255)
                                                               tgatATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGGCGGGGGGGGGAATTATCAGGGATGGGTTACTGCACAAAACCCCGC‑GGGAAATCCTCTGGA‑TCaaaaaat                                                                                        >  2:1134446/1‑148 (MQ=255)
                                                               tgatATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGCC‑GGTTGGGCGGGGGGGGGGAATTTCCGCGATTGATTACCGCACAACACCCCGC‑GGGAATTTCTCCGGA‑ACcaaaaaa                                                                                        >  2:569491/1‑148 (MQ=255)
                                                                 atatACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑TGGTGGTCGGGGGGGGGGATTATTCGCGATGGAGTACTGCACAACACCCCGC‑GGAAAATTCTCTGGATTCaaaaatta                                                                                       >  1:884698/1‑147 (MQ=255)
                                                                 atatACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGGGGGGAATTATCAGCGATGGGTTACTGCACAAAACCCCGC‑GGGAATTCCTCTGGG‑TCAAAAAATCa                                                                                      >  2:271610/1‑150 (MQ=255)
                                                                 atatACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGGCGGGGGGGGGGATTATTAGCGATGGATTACTGCACAAAACCCTGC‑GGGAAATTCTCTGGA‑TTAAAAACTCa                                                                                      >  1:1277380/1‑150 (MQ=255)
                                                                 atatACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGGCGGGGGGGGGAATTATCAGCGATGGATTACTGCCCAAAACCCCGC‑GGGAAATCCTCTGGA‑TCAAAAAATCa                                                                                      >  2:70622/1‑150 (MQ=255)
                                                                  tataCGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCAcc                                                                                    <  2:524677/151‑1 (MQ=255)
                                                                   ataCGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCAccc                                                                                   <  2:1260157/151‑1 (MQ=255)
                                                                   ataCGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCAccc                                                                                   <  2:698960/151‑1 (MQ=255)
                                                                   ataCGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCAccc                                                                                   <  1:1352571/151‑1 (MQ=255)
                                                                      cGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGGT‑TGGTGGGCGGGGGGGGGGATTATCAGCGCTGGATTACTGCCCAAAACCCTGC‑GGAAAATTCTCTGGG‑TCAAAAACTCACCCa                                                                                  >  1:842459/1‑149 (MQ=255)
                                                                        tctcTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCACCCATTa                                                                               <  2:1577088/150‑1 (MQ=255)
                                                                        tctcTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCACCCATTAc                                                                              <  1:1398557/151‑1 (MQ=255)
                                                                            tGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCACCCATTACGAAc                                                                          <  1:1048136/151‑1 (MQ=255)
                                                                                 ggAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGGGGGGAATTATCAGCGATGGGTTACTGCACAACACCCTGC‑GGAAATTTCTCTGGG‑TCAAAAAATCACCCCTTACGaaaaa                                                                        >  2:1044788/1‑145 (MQ=255)
                                                                                 ggAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGGTGGGGGGGGGGGGGGAATAAAAACGATGGTTTACTGCCCAACAACCTTC‑GGAAATTTCCCTGGA‑TAAAAAAATTTCCCATTACGAACAGCtt                                                                     >  2:1055314/1‑150 (MQ=255)
                                                                                 ggAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATAATGGCGGTT‑GGGTGGGCGGGGCGGGGGATTTTCAGCGGTGGAATACTGAACTAAACCCCGC‑GGAAATTGCTCTGGG‑TCAAAAAACCACCCATTACgcaaag                                                                        >  2:1677182/1‑143 (MQ=255)
                                                                                  gaagaaGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGGTGGTCGGGGGGGGGGATTTTTAGCGATGGATTATCTCACAACCCCCCTC‑GGAAATTTCTCCTGG‑TCCAAAAATCACCCATTACGAACACCTgg                                                                    >  1:725281/1‑151 (MQ=255)
                                                                                   aagaagATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGGCGGGGGGGGGAATTTTCAGCCATGGGTTACTGGACCACACCCCTC‑GGAAATTCCTCTTGG‑TCAAAAAATCCCCCATTACGAACAGCTgg                                                                    >  2:1176009/1‑150 (MQ=255)
                                                                                      aagaTGTGCTCTACGCCCTGACGGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGCAATTATCAGCGATGGATTACTTCACATCTCCCTGC‑GGAAATACCTCTGGT‑ACGAAAACTCCCCCTTTTCGAGCAGCTgaaacg                                                                <  1:453197/151‑6 (MQ=255)
                                                                                      aagaTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGGGGGGAATTATTAGCGATGGATTACTGCCCAAAACCCTGC‑GGGAATTCCTTTGGA‑TCAAAAAATCACCCCTTACGCACAGCTGGCCAg                                                                >  2:1126997/1‑151 (MQ=255)
                                                                                        gaTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑TGTTGGGCGGGGAGGGGGATTATTAGCGGTGGATTACTGCCCAACACCCTGC‑GGGAATTCCTCTGGG‑TCAAAAACTCACCCATTACGAAAAACTGGCAAggg                                                              >  2:387646/1‑149 (MQ=255)
                                                                                        gaTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGGTGGTCGGGGGGGGGAATTATTAGCGATGGATTACTGCACAACACCCTGC‑GGGAATTTCTCTGGG‑TCAAAAACTCACCCCTTACGAACAGCTGGCAAGTg                                                              >  2:1414428/1‑151 (MQ=255)
                                                                                        gaTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGGTGGGCGGGGGGGGGAATTATCAGCGATGGATTACTGGACAAAACCCTGC‑GGGAATTCCTCTGGA‑TCAAAAACTCACCCATTACGAACACCTGGCAAggg                                                              >  1:447831/1‑149 (MQ=255)
                                                                                           gtgCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑TGGTGGTCGGGGGGGGGAATTATCAGCGATGGGTTTCTGCCCAACACCCCGC‑GGAAATTCCCCTTGG‑GCAAAAAATCACCCATTACAAACAGCTGGGCAGGGATg                                                           >  2:108972/1‑151 (MQ=255)
                                                                                            tactctACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGc                                                          <  2:222454/149‑1 (MQ=255)
                                                                                              ctctACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGGGGGGGATTATTACGGGTGGATTACTGCCCAACACCCTCC‑GGGAATTCCTCTGGG‑TCAAAAACCCACCCAATACAAACAGCTGGCAAGCGAAGCa                                                         >  1:925672/1‑150 (MQ=255)
                                                                                              ctctACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCAc                                                        <  1:238745/151‑1 (MQ=255)
                                                                                              ctctACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGGTGGGCGGGGGGGGGGATTATCAGCGATGGATTACTGCACAAAACCCCGC‑GGGAAATTCTCTGGA‑TCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCa                                                         >  1:1748909/1‑150 (MQ=255)
                                                                                               tctACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑TGTTGGGCGGGGGGGGGGATTATCAGCCATGGGTTTCTCCACAACACCCTGC‑GGAAATTTCTCTGGG‑TCAAAAACCCCCCCATTACCAACCGCTGGCAAGAGATGcc                                                         >  2:1413314/1‑148 (MQ=255)
                                                                                               tctACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGGCGGGGGGGGGGATTATCAGCGATGGATTACTGCACAACACCCCGC‑GGGAATTTCTCTGGG‑TCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCa                                                         >  1:1125782/1‑149 (MQ=255)
                                                                                                ctACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGGGGGGGATTATCTGCGGAGGGTTACTGCCCAAAACCCTGC‑GGGAAATTCTCTGGA‑TCAAAAAATCACCCCCTACGAACAGCTTGCAAGTGATGCAccg                                                      >  1:1217588/1‑149 (MQ=255)
                                                                                                     cccTGACCGTATTTAACGGTGAATATACTGGCGGGT‑GGTTGGTCGGGGGGGGGGATTATCAGCGATGGATTACTTGACAACACCCCTC‑GGGAATTCCTCTGGG‑TCAAAAAATCAACCATTACGAACAGCTGGGAAGAGATTCAATGGCCgg                                                 >  1:876647/1‑151 (MQ=255)
                                                                                                             gTATTTAACGGTGAATATACTGGCGGTT‑TGTTGGTTGGGGGGGGGGATTATCAGGGATGGATTACTGCCCAACACCCTGC‑GGGAAATTCTTTGTA‑ACAAAAAACCACCCAATACCAACAGCTGGCAAGAGATGGACAGGCAGGAGaaaatt                                         >  1:73772/1‑148 (MQ=255)
                                                                                                             gTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGGATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGGAATTCCTCTGGA‑TCAAAAAATCACCCATTACGAACAGCCGGCAAGTGATGCACTGGCAGGAGAAAAtg                                         >  1:76631/1‑151 (MQ=255)
                                                                                                               aTTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGGGGGGGATTTTCAGCGATGGATTACTTCCCAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAAATCACCCATTACGAACAGCTGGCAAGGGATGCACTGGCAGGGGAAATtgtg                                       >  2:209745/1‑151 (MQ=255)
                                                                                                               aTTTAACGGTGAATATACTGGCGGTT‑GGGTGGTCGGGGGGGGGAATTATCAGCGATGGGTTAATGCACAAAACCCCGC‑GGGAATTTCTCTGGA‑TTCAAAACTCACCCATTACGAAAAACCGGCAAATGAAGCACTTGCAGGGGAAATtttg                                       >  2:552990/1‑151 (MQ=255)
                                                                                                                   aaCGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGAGAAATTGTGGGtt                                   <  1:1772104/151‑1 (MQ=255)
                                                                                                                           atatACTGGCGGTT‑GGTTGGTGGGGGGGGGGGATTTTCACCGATGGATTACTGGCCAAAACCCCGC‑GGGAATTTCTCTTGA‑TCAAAAAATCACCCATTACGAACAGCTGGCAAGTGTTGCACTTGCTGGGGAAAATTTGGGTTCTTTTgcg                           >  1:217554/1‑151 (MQ=255)
                                                                                                                           atatACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTTGCAGGAGAAATTTTGGGTTCTTCTgcg                           >  1:341994/1‑151 (MQ=255)
                                                                                                                            tataCTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGAGAAATTGTGGGTTCTTCTgcgg                          <  1:921190/151‑1 (MQ=255)
                                                                                                                                      gtt‑ggttgGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGAGGAATTGTGGGTTCTTCTGCGGGCGGCGagc                 <  1:518815/150‑1 (MQ=255)
                                                                                                                                              ggtCGGGGCGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGAGAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCaaaa         >  2:323651/1‑151 (MQ=255)
                                                                                                                                              ggtCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGAGAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCaaaa         >  2:863825/1‑151 (MQ=255)
                                                                                                                                                tCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGAGAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAAtt       <  1:387522/151‑1 (MQ=255)
                                                                                                                                                tCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGAGAAATTGTGGGTTCTTCTGCAGGCGGCGAGCAGCCAAAAtt       <  2:47290/151‑1 (MQ=255)
                                                                                                                                                   gggaggggaATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGAGAAATTGAGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAAtttt     >  2:1759544/1‑149 (MQ=255)
                                                                                                                                                     gaggggaATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGAGAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTAcc   <  1:266192/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
ACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTT‑GGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGC‑GGAAATTCCTCTGGA‑TCAAAAACTCACCCATTACGAACAGCTGGCAAGTGATGCACTGGCAGGAGAAATTGTGGGTTCTTCTGCGGGCGGCGAGCAGCCAAAATTTACCT  >  NC_000913/4622101‑4622399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑