Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 720,946 C→T intergenic (+106/+110) ybfK → / ← kdpE uncharacterized protein/response regulator in two‑component regulatory system with KdpD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913720,9460CT100.0% 203.7 / NA 52intergenic (+106/+110)ybfK/kdpEuncharacterized protein/response regulator in two‑component regulatory system with KdpD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/48);  total (4/48)

TAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGT  >  NC_000913/720801‑721091
                                                                                                                                                 |                                                                                                                                                 
tAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTaa                                                                                                                                              <  2:578228/151‑1 (MQ=255)
   tGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAatat                                                                                                                                           <  2:914231/151‑1 (MQ=255)
   tGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAatat                                                                                                                                           <  2:395600/151‑1 (MQ=255)
     cGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATaa                                                                                                                                         <  1:610517/151‑1 (MQ=255)
     cGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATaa                                                                                                                                         <  1:1517867/151‑1 (MQ=255)
     cGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATaa                                                                                                                                         <  1:1481351/151‑1 (MQ=255)
     cGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATaa                                                                                                                                         <  2:143619/151‑1 (MQ=255)
        cTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAat                                                                                                                                      <  1:1113156/151‑1 (MQ=255)
           ttATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAatat                                                                                                                                    <  1:1176268/150‑1 (MQ=255)
                          tttaCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTtg                                                                                                                    <  1:233448/151‑1 (MQ=255)
                          tttaCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTtg                                                                                                                    <  1:721882/151‑1 (MQ=255)
                          tttaCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTtg                                                                                                                    <  2:1629021/151‑1 (MQ=255)
                                    cTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGttt                                                                                                          <  1:553453/151‑1 (MQ=255)
                                          tgaatgaaGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGAt                                                                                                     <  1:1196896/150‑1 (MQ=255)
                                          tgaatgaaGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGAt                                                                                                     <  1:227995/150‑1 (MQ=255)
                                                aaGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGc                                                                                              <  1:1372857/151‑1 (MQ=255)
                                                aaGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGc                                                                                              <  2:1423661/151‑1 (MQ=255)
                                                      aaGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtatt                                                                                        >  2:347117/1‑151 (MQ=255)
                                                      aaGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtat                                                                                         <  2:1717179/150‑1 (MQ=255)
                                                      aaGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtat                                                                                         >  1:1716352/1‑150 (MQ=255)
                                                          tatTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtatttatt                                                                                    <  1:653169/151‑1 (MQ=255)
                                                          tatTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtatttatt                                                                                    <  2:365499/151‑1 (MQ=255)
                                                          tatTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtatttatt                                                                                    <  1:1101981/151‑1 (MQ=255)
                                                           atTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATttatta                                                                                   <  1:1058940/151‑1 (MQ=255)
                                                            ttCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATttattat                                                                                  <  1:1593477/151‑1 (MQ=255)
                                                               tATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTAc                                                                               <  1:1589764/151‑1 (MQ=255)
                                                                      gAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTtta                                                                        >  2:1259393/1‑151 (MQ=255)
                                                                            cGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTtaattaat                                                                  >  1:1297014/1‑151 (MQ=255)
                                                                               ttGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATtaataa                                                                <  1:698057/150‑1 (MQ=255)
                                                                               ttGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATtaataa                                                                <  2:18396/150‑1 (MQ=255)
                                                                                   aCTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAaggc                                                           <  1:109314/151‑1 (MQ=255)
                                                                                       tgAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAaggcaggc                                                       <  1:478518/151‑1 (MQ=255)
                                                                                             tGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTAtt                                                 <  1:201146/151‑1 (MQ=255)
                                                                                             tGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTAtt                                                 <  1:718394/151‑1 (MQ=255)
                                                                                                 cAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTaaaa                                             <  1:1340858/151‑1 (MQ=255)
                                                                                                 cAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTGATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTaaaa                                             <  1:1246255/151‑1 (MQ=255)
                                                                                                  aaTAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAAt                                            <  2:1613267/151‑1 (MQ=255)
                                                                                                  aaTAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAAt                                            <  1:1412659/151‑1 (MQ=255)
                                                                                                  aaTAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAAt                                            <  1:907265/151‑1 (MQ=255)
                                                                                                  aaTAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAAt                                            <  2:948925/151‑1 (MQ=255)
                                                                                                                      aaTTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTGTTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAAcc                        <  2:1466094/151‑1 (MQ=255)
                                                                                                                      aaTTTATTTAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAAcc                        <  1:1481071/151‑1 (MQ=255)
                                                                                                                       atttatttAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCg                       <  1:1102851/151‑1 (MQ=255)
                                                                                                                       atttatttAATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCg                       <  1:981777/151‑1 (MQ=255)
                                                                                                                           atttaATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTGATATTCAAAGCATAAACCGATAg                   <  1:1406240/151‑1 (MQ=255)
                                                                                                                             ttaATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGcc                 <  1:1071550/151‑1 (MQ=255)
                                                                                                                             ttaATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGcc                 <  2:677080/151‑1 (MQ=255)
                                                                                                                             ttaATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGcc                 <  1:669192/151‑1 (MQ=255)
                                                                                                                             ttaATGATGAGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGcc                 <  1:263815/151‑1 (MQ=255)
                                                                                                                                   atgaGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATAc            <  2:1172810/150‑1 (MQ=255)
                                                                                                                                      aGCTTTTTACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCgg         <  2:1652216/150‑1 (MQ=255)
                                                                                                                                            ttACTTAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGt  <  2:150574/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGT  >  NC_000913/720801‑721091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑