Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,640,9810CT49.5% 47.0 / 72.6 100F40F (TTC→TTT) dtpBdipeptide and tripeptide permease B
Reads supporting (aligned to +/- strand):  ref base C (10/40);  new base T (49/0);  total (60/40)
Fisher's exact test for biased strand distribution p-value = 1.49e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

TCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACCTGCT‑GGGGACCAAACGCACCATTGTTCTTGGAGCAC  >  NC_000913/3640834‑3641126
                                                                                                                                                     |                                                                                                                                                        
       ggTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTCT‑TTtaaa                                                                                                                                               >  1:1071463/1‑150 (MQ=255)
       ggTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑Gttttttttgtaaaa                                                                                                                                                >  1:314010/1‑143 (MQ=255)
        gTATTGGAAGGTTAATGAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACAGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTTG‑TTtaaaa                                                                                                                                              >  1:1661671/1‑149 (MQ=255)
        gTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTTG‑TTTAAC‑a                                                                                                                                             >  2:603262/1‑151 (MQ=255)
        gTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTTC‑TTTAAC‑a                                                                                                                                             >  1:1466861/1‑151 (MQ=255)
        gTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTCG‑TTtaaaa                                                                                                                                              >  2:782729/1‑149 (MQ=255)
        gTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑GTTTTTTTG‑TTtaaaa                                                                                                                                              >  2:602509/1‑149 (MQ=255)
        gTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑GTTTTTTCT‑TTtaaaa                                                                                                                                              >  2:911916/1‑149 (MQ=255)
        gTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑GTTTTTTCT‑TTAAAC‑a                                                                                                                                             >  1:928751/1‑151 (MQ=255)
        gTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑GTTTTTTCG‑TTtaaaa                                                                                                                                              >  2:1071124/1‑149 (MQ=255)
        gTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑GTTTTTTCG‑TTtaaa                                                                                                                                               >  1:1108134/1‑149 (MQ=255)
         tATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑Ag                                                                                                                                            <  1:487505/151‑1 (MQ=255)
          aTTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGc                                                                                                                                           <  1:1346947/151‑1 (MQ=255)
           ttGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTTG‑TTtaaaaa                                                                                                                                             >  1:299204/1‑146 (MQ=255)
             ggAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGT‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTg                                                                                                                                        >  2:1586669/1‑151 (MQ=255)
                  gTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTTG‑TTTAAC‑AGCCTgggatt                                                                                                                                   >  1:746813/1‑147 (MQ=255)
                  gTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTTG‑TTTAAA‑AGATTGGGttt                                                                                                                                   >  2:455564/1‑150 (MQ=255)
                  gTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGAtt                                                                                                                                    <  2:311281/150‑1 (MQ=255)
                  gTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGAt                                                                                                                                     <  2:1487950/149‑1 (MQ=255)
                  gTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑GTTTTTTTT‑TTTAAC‑AACTTGGGtt                                                                                                                                    >  1:1664330/1‑150 (MQ=255)
                  gTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑GTTTTTTTG‑TTAAAA‑AACTTGGGttt                                                                                                                                   >  2:477851/1‑150 (MQ=255)
                  gTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑GTTTCTTTG‑TTAAAA‑AGCtttggttt                                                                                                                                   >  2:1169450/1‑145 (MQ=255)
                      ataaataTGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTTG‑TTTAAA‑AGCTTTGGTtcttg                                                                                                                                >  1:775530/1‑148 (MQ=255)
                        aaataTGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCCTTT‑TTTAAA‑AGCTTgtgtttcttcc                                                                                                                              >  1:1565024/1‑140 (MQ=255)
                         aataTGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑GTTTTTTTG‑TTTAAA‑AACTTGGGTTTT‑CGCCag                                                                                                                           >  2:223636/1‑151 (MQ=255)
                          atatGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTTG‑TTTAAA‑AACTTGGGTTTT‑CGCaaag                                                                                                                          >  1:738389/1‑149 (MQ=255)
                           tatGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGG‑TTTTATTGG‑TTAAA‑‑AACCTGGGTTTT‑CGCCagag                                                                                                                         >  2:1685241/1‑151 (MQ=255)
                            atGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGc                                                                                                                        <  2:603723/151‑1 (MQ=255)
                              gAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCCGGGCGTACTGGC‑GGCTTTTTTTTG‑TTAAAA‑ACCTTGG‑Ttct‑cccccgggccg                                                                                                                      >  2:1685490/1‑141 (MQ=255)
                              gAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCC‑TTTAAA‑AGCCTTGGTTTT‑CCCAAAAGCA‑g                                                                                                                     >  2:437151/1‑151 (MQ=255)
                                aTACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGc                                                                                                                   <  1:603001/151‑1 (MQ=255)
                                 tACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCt                                                                                                                  <  1:929325/151‑1 (MQ=255)
                                   caacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGG‑TTTTTTTGG‑TTAAA‑‑AACTTGGGTTTT‑CGCAAGAGCA‑GGCctt                                                                                                                >  2:110458/1‑151 (MQ=255)
                                   caacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTTG‑TTTAAA‑AGCTTTGATTTT‑CGCAAGAGGA‑GGCttt                                                                                                                >  2:24789/1‑151 (MQ=255)
                                   caacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTTG‑TTTAAA‑AACTTGGGTTTT‑CGCCAGAGCA‑GGCttt                                                                                                                >  2:408164/1‑151 (MQ=255)
                                   caacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTCG‑TTTAAA‑AGCTTGGGTTTT‑CGCCAGAGCA‑GGCttt                                                                                                                >  2:143293/1‑151 (MQ=255)
                                   caacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTCC‑TTTAAC‑AGGCTGGAGTCT‑CGCCAGAGCA‑GGCttt                                                                                                                >  1:730126/1‑151 (MQ=255)
                                   caacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑GTTTTTTTT‑TTTAAC‑AGCTTTGGTTTT‑CGCCAGAGCA‑ggggtt                                                                                                                >  2:1394721/1‑147 (MQ=255)
                                   caacaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑GTTTCTTCG‑GTAAAA‑AGTTTGGGATAT‑TGCAAGAGAA‑GGGttt                                                                                                                >  1:1728852/1‑151 (MQ=255)
                                     acaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTTT‑TTTAAA‑AGCTTTGGTTTT‑TGCCAAAGAA‑GGGttttt                                                                                                              >  2:1398813/1‑150 (MQ=255)
                                     acaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTTG‑GTTAAA‑AGGTTGGGTTTT‑TGCCCAAGCA‑GGGttttt                                                                                                              >  2:581383/1‑150 (MQ=255)
                                     acaacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAA‑AGCTTGGATTCT‑CGCCAGAGCA‑GGGttttt                                                                                                              >  2:927216/1‑150 (MQ=255)
                                       aacaCCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTc                                                                                                            <  2:1621097/151‑1 (MQ=255)
                                         cacCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑GTTTTTTTG‑TTTAAA‑AACTTGGGTTTT‑CGCCAGAGCAGGGCttttttg                                                                                                            >  1:1700522/1‑147 (MQ=255)
                                         cacCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGCGCAGGGCGTACTGGC‑GG‑TTTTTTTTG‑TTTAAA‑AACTTGGGTTCT‑CGCAAGAGGAGGGCTTTTTTc                                                                                                            >  2:1200641/1‑150 (MQ=255)
                                          acCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTCG‑TTTAAA‑AGCTTGGGTTTT‑CGCAAGAGAA‑GGGTTTTTTCa                                                                                                           >  1:1406949/1‑149 (MQ=255)
                                           cccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGG‑TTTTTTTCT‑TTAAA‑‑AACTTGGGTTTT‑CCCAAGAGCA‑GGCTTTTTTTACtt                                                                                                        >  1:191029/1‑151 (MQ=255)
                                           cccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTTGTTTAAAA‑ACCTTGG‑TTCT‑CCCCAGAACAGGGCTTTTTTcc                                                                                                           >  2:1122242/1‑148 (MQ=255)
                                           cccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTTG‑TTAAAA‑AACTTGGTTTCTCCGCAA‑AGCAGGGCTTTTTTcctt                                                                                                         >  2:728441/1‑148 (MQ=255)
                                           cccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTTAACAACCTTGGGTT‑T‑CCCCAGAGCA‑ggggtttttttctt                                                                                                        >  1:150731/1‑139 (MQ=255)
                                           cccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑GTTTTTTCG‑TTTAAA‑AACTTGGGTTTT‑CGCCAGAGGA‑GGGCTTTTTCACtt                                                                                                        >  2:135576/1‑151 (MQ=255)
                                           cccATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑GTTTCGTCG‑TTAAAC‑ACTCTGGGTTCT‑CGAAATAACA‑CGGTTTTgctgtgt                                                                                                        >  1:901120/1‑145 (MQ=255)
                                                      gctgcAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTTG‑TTTAAA‑AACCTGGGTTCT‑CGCCAAAGGA‑GGCTTTTTTTCCTTTTTGGGGtttt                                                                                             >  2:486914/1‑151 (MQ=255)
                                                       ctgcAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGG‑TTTTTTTGT‑TTACTA‑ACCTTGGTTTCC‑TCCAGGGGAA‑GGCTTTTTCCACTTTTGTGGCtttt                                                                                             >  1:1137228/1‑151 (MQ=255)
                                                         gcagcaACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑GTTTTTTTG‑TTAAAA‑AACTTTGGTTTT‑CGCAAGAGGA‑GGCTTTTTTTAATTTTGGGGGttttt                                                                                            >  2:101490/1‑148 (MQ=255)
                                                           agcaACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTTG‑TTTAAC‑AACTTTGGTTCT‑CGCCAGAGGA‑GGGTTTTGTTACTTTTGGTGCTTTttctgg                                                                                        >  1:1499171/1‑150 (MQ=255)
                                                             caACCTCGCCCATTTTTCATGATCTTTGTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTgcgc                                                                                      <  1:370576/151‑1 (MQ=255)
                                                                  tCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTc                                                                                 <  1:1472799/151‑1 (MQ=255)
                                                                  tCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTc                                                                                 <  1:259818/151‑1 (MQ=255)
                                                                  tCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTc                                                                                 <  2:258807/151‑1 (MQ=255)
                                                                    gCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTCG‑TTTAAA‑AGCTTGGGTTTT‑CGCAAGAGCA‑GGGTTTTTTTACTTTTTGGGCTTTTTCTTCGCTGGGCtt                                                                               >  2:1061035/1‑150 (MQ=255)
                                                                       cATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATgg                                                                            <  2:1311379/151‑1 (MQ=255)
                                                                       cATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATgg                                                                            <  2:1665171/151‑1 (MQ=255)
                                                                       cATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATgg                                                                            <  2:271205/151‑1 (MQ=255)
                                                                         tttttCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGcc                                                                          <  1:408017/151‑1 (MQ=255)
                                                                                  aTCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTcc                                                                  <  2:652662/150‑1 (MQ=255)
                                                                                   tCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCAt                                                                <  1:223674/151‑1 (MQ=255)
                                                                                      tttttGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTTG‑TTTAAC‑AACTTGGGGTTT‑CGCAAGAGGA‑GGCGTTTTTTCCTTTTTGGGCTTTTTTTTCGCTGGGCTCTGGCCTCCTTTTCCTTgg                                                             >  1:1686354/1‑151 (MQ=255)
                                                                                               aGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGGGTACTGGC‑GG‑TTTTTTTTG‑GTTAAA‑AGCTTGTGATCT‑CGAGACAGAA‑GGAGTTTTTGTCTTTTGGGGTTTTTTGTGGGCTGGTCTGTGGGCCCTTTTTCCTTggcgggcgtgg                                                    >  1:1079038/1‑145 (MQ=255)
                                                                                                gTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑CGTTCTTTG‑GTTAAA‑AGGTTGGGTTTT‑CCCCAGAGGA‑GGCGGTTGTCACCTTTGGTGGTTTTGCTGCGCTGGTTTCTTGCCTCTTTTTCCTTtgcgggaatgc                                                    >  2:924756/1‑144 (MQ=255)
                                                                                                 ttATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGt                                                    <  1:1050199/149‑1 (MQ=255)
                                                                                                   aTGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑GTTTTTTTG‑TTTAAA‑AGATTGGGTTCT‑CGCCAGAGCA‑GGCTTTTTTTACTTTTTGGGGTTTTTCTTCGCTGGGCTATGGGCTCATTTTCATTTGCGGGTAtttttgg                                                >  1:1224277/1‑145 (MQ=255)
                                                                                                       gAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTCC‑TTTAAC‑AGCTTGGATTTT‑CGCAAGAGCC‑GGCGTTTTTCCCTTTTTGTGCTTTTTTTTCGCTGGGCTATGGCCTCCTTTTCCTTggcgggtcttttgggggc                                             >  1:1632428/1‑137 (MQ=255)
                                                                                                       gAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGac                                           <  2:1574556/150‑1 (MQ=255)
                                                                                                          cGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGaccacc                                       <  1:1559250/151‑1 (MQ=255)
                                                                                                             ttCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACctgc                                    <  1:1181471/151‑1 (MQ=255)
                                                                                                             ttCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACctgc                                    <  1:143247/151‑1 (MQ=255)
                                                                                                               cGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACctgct‑g                                 <  1:1195071/151‑1 (MQ=255)
                                                                                                               cGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACctgct‑g                                 <  2:217373/151‑1 (MQ=255)
                                                                                                               cGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACctgct‑g                                 <  2:1477766/151‑1 (MQ=255)
                                                                                                                ggCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTTG‑TTAAAA‑AGCTTGGGTTCT‑CGCCAGAGCA‑GGGTTTTGTTACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCCCCTTTCCATTGGGGGCGATTT‑C‑CGGGACCACCCGCC‑gg                                >  1:1144268/1‑151 (MQ=255)
                                                                                                                    actacGGCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTCC‑TTTAAA‑AGCTTGGGTTCT‑CGCCAAAGCA‑GGCTTTTTTCACTTTTTGTGCGTTTTCTCCGCTGGGCTTTGGGCCCCTTTTCATTTGCGGCCTTTT‑C‑GGGGgccaccccctcgggg                              >  2:308893/1‑141 (MQ=255)
                                                                                                                    actacGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACCTGCT‑GGGGAc                            <  1:270083/151‑1 (MQ=255)
                                                                                                                          gCGTGCAGGGCGTACTGGC‑GG‑TTTTTTTCG‑TTAAAC‑AGCTTGGAGTCT‑CGCAAGAGCC‑GGGTTTTGTTACCTTTTGTGGTTTTTCTGCGCTGGGCTATGGCCTCCTTTTCATTTGGGGCTATTT‑C‑GGCGACCCCCCGCT‑GGGGAGCaaaa                       >  1:1299031/1‑149 (MQ=255)
                                                                                                                          gCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACCTGCT‑GGGGACCAAAc                       <  1:1070570/150‑1 (MQ=255)
                                                                                                                           cGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACCTGCT‑GGGGACCAAACg                      <  1:20587/150‑1 (MQ=255)
                                                                                                                           cGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACCTGCT‑GGGGACCAAACGc                     <  2:305791/151‑1 (MQ=255)
                                                                                                                            gTGCAGGGCGTACTGGC‑GG‑TTTTTTTCG‑TTAAAC‑AGCTTGGGTTTT‑CGCAAGAGCA‑GGGGTTTGTCACTTTTTGTGGTTTTGCTGCGCTGGTCTATGGCCTCATTTTCATTGGCGGGCATGT‑CGGGGGCCCACCCCCT‑GGGGGCAAAAcc                      >  1:592876/1‑149 (MQ=255)
                                                                                                                                 gggCGTACTGGC‑GG‑TTTTTTTCG‑TTTAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTTACTTTTTGTGCTTTTTCTGCGCTGGGCTATGGCCTCATTTTCATTGGCGGGCATTT‑C‑GGCGGCCCCCCGCTGGGGGCCCAAACCCAcatt                >  1:631778/1‑148 (MQ=255)
                                                                                                                                 gggCGTACTGGC‑GG‑TTTTTTTCG‑TTAAAC‑AGCTTGGATTTT‑CGCAAGAGCA‑GGCTTTTTTTACTTTTTGGGCTTTTTCTGCGCTGGGCTATGGCCTCCTTTTCCTTGGCGGCTCTTTGG‑GGGGCCCCCCTGTT‑GGGGGCCaaagccccc                  >  2:1254514/1‑143 (MQ=255)
                                                                                                                                 gggCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAACAGCA‑GGCTTTTTTCACTTTTGGTGCTTTTTCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGGTATTT‑C‑GGCGGCCCCCCGCT‑GGGGGCCAAAAGCACCAtt               >  2:1046165/1‑151 (MQ=255)
                                                                                                                                  ggCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACCTGCT‑GGGGACCAAACGCACCAt                <  2:776120/149‑1 (MQ=255)
                                                                                                                                      tACTGGC‑GT‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTTTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACCTGCT‑GGGGACCAAACGCACCATTGTTc           <  2:1729748/150‑1 (MQ=255)
                                                                                                                                      tACTGGC‑GG‑TTTTCTTCG‑TTAATC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACCTGCT‑GGGGACCAAACGCACCATTGTTc           <  1:1320924/150‑1 (MQ=255)
                                                                                                                                      tACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACCTGCT‑GGGGACCAAACGCACCATTGTTc           <  1:1642347/150‑1 (MQ=255)
                                                                                                                                      tACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACCTGCT‑GGGGACCAAACGCACCATTGTTc           <  1:418738/150‑1 (MQ=255)
                                                                                                                                      tACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACCTGCT‑GGGGACCAAACGCACCATTGTTc           <  1:1019826/150‑1 (MQ=255)
                                                                                                                                       aCTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACCTGCT‑GGGGACCAAACGCACCATTGTTCtt         <  1:110426/151‑1 (MQ=255)
                                                                                                                                            c‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACCTGCT‑GGGGACCAAACGCACCATTGTTCTTGGAGc    <  2:1407611/151‑1 (MQ=255)
                                                                                                                                                 ttttCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACCTGCT‑GGGGACCAAACGCACCATTGTTCTTGGAGCAc  <  1:728069/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                        
TCAGGATGGTATTGGAAGGTTAATAAATATGAATACAACAACACCCATGGGGATGCTGCAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGC‑GG‑TTTTCTTCG‑TTAAAC‑AGCTTGGATTCT‑CGCAAGAGCA‑GGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTCATTTCCATTGGCGGCTATGT‑C‑GGCGACCACCTGCT‑GGGGACCAAACGCACCATTGTTCTTGGAGCAC  >  NC_000913/3640834‑3641126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑