Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,665,6980TC57.3% 33.9 / 178.8 144D38G (GAT→GGT) gadXacid resistance regulon transcriptional activator; autoactivator
Reads supporting (aligned to +/- strand):  ref base T (42/19);  new base C (0/82);  total (42/102)
Fisher's exact test for biased strand distribution p-value = 1.02e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.02e-16

Only 100 of 144 total aligned reads displayed.

ACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGT  >  NC_000913/3665550‑3665843
                                                                                                                                                           |                                                                                                                                                  
acAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATc                                                                                                                                                   >  2:259851/1‑150 (MQ=255)
   gTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCa                                                                                                                                               >  1:433220/1‑151 (MQ=255)
   gTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCa                                                                                                                                               >  1:997753/1‑151 (MQ=255)
   gTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCa                                                                                                                                               >  1:900022/1‑151 (MQ=255)
           cGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAg                                                                                                                                      >  1:1020638/1‑151 (MQ=255)
             aGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGt                                                                                                                                    >  2:1158613/1‑151 (MQ=255)
              gTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGt                                                                                                                                    >  2:904239/1‑150 (MQ=255)
              gTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGt                                                                                                                                    >  1:438405/1‑150 (MQ=255)
                          cTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTaaa                                                                                                                       >  1:1299237/1‑151 (MQ=255)
                          cTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTaaa                                                                                                                       >  1:436717/1‑151 (MQ=255)
                          cTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTaaa                                                                                                                       >  1:1030432/1‑151 (MQ=255)
                            tttttATGTGCATGAAGATTTAATGCCTCCCCCTTGAGCATCGGGAAAAATAATAAAAGCGTGCCCTTTGACACAAAAAAAAAATTTCCAACCCCTTTATCTACTAGAATTTGGCTTGCACCCGAAAAAACCGGGTCACCGCCATTAAAAt                                                                                                                     <  1:494201/151‑1 (MQ=255)
                                   gtgCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGata                                                                                                              >  1:1667521/1‑151 (MQ=255)
                                        tGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGTCATTAAAATAGCGATATTCAc                                                                                                         <  1:1163962/151‑1 (MQ=255)
                                         aaaGATTTAATCCCCCCCCCATGAGCAGCGGAAAAAAAAATGAAACCGTTCCCTTTGCCCAGAAAACAAAATTTACACCCCCCTTATCAACTGAAATTGGCTTGGCCCCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCAcc                                                                                                        <  1:60806/150‑1 (MQ=255)
                                           aattttAATCCCCCCTCCTTGACCTTGGGAAAAAATAATGAAAGCTTGTCCCTTGACCCAAATAAAAAATTTCCAACACCCTTATCTCCTCAATTTTGGCTTCCCCCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCAt                                                                                                      <  2:1525216/148‑1 (MQ=255)
                                            gATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCAtt                                                                                                     >  2:1456429/1‑151 (MQ=255)
                                             aTTTAATCCCTCCTCCTGAACCTTGGGGAAAAATAATAAAAGCGTTCCCTTGGACAGAAAAAAAAAATTTAAACCCCCTTAACCTCACCAATTTGGCTTTGCACCCCAAAAAACCAGGCCACCGCCATTAAAATAGCGATATTCACCAt                                                                                                      <  1:1013247/149‑1 (MQ=255)
                                               ttatcccctccccctTGAGCAGCGGAAAAAATAATAAAAGCGTGCCCCTTGACACAAATACAAAATTTTCACCACCCTTATCTCCTCAATTTTGTCTTGCACCCCCAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAc                                                                                                  <  1:324130/145‑1 (MQ=255)
                                                tatccccccctcctTGAGCACGGGGAAAAATAAAAAAAGGGTTCCCTTTGACACAAAAAAAAATTTTCCAACCCCTTTATCTCCCCAATTTTGGCTTCCATCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                 <  2:376732/143‑1 (MQ=255)
                                                taagccctccccctTGAGCATGGGGAAAAATAATGAAAGCGTCCCCCTTGACACAAATAAAAAATTTTCAACACACTTATCTACTCAAATTTGGCTTCCACCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                 <  2:1314846/146‑1 (MQ=255)
                                                tAATGCCTCCTCCTTGTGCAGGGGAAAAAAAAAGAAAGGCGTGCCCCTGGACAAAAAAACAAAATTTCCACCCCACTTATCTACTAAATTTGGCCTTGCACCCAAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                 <  1:1364978/151‑1 (MQ=255)
                                                tAATGCCTCCTCCTTGAGCATCGGGAAAAATAATGAAAGCGTGTCCCTTGCCACAAAAAAAAAATTTTCAACACACTTATCTCCTCGAATTTGGCTTGCACCCGAAAAAACCAGGACACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                 <  2:1362252/151‑1 (MQ=255)
                                                tAATCCCCCCTCCTGGAGAATGGGAAAAAAAAATGAAGGCTTTTCCCTTAACACAAAAAAAAAATTTTAAACCCATTTATCTCCTCAATTTTGGCTTCCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                 <  2:1710800/151‑1 (MQ=255)
                                                tAATCCCCCCTCCTGGAAAATGGGGAAAAATAATAAAGGCGTGTCCCTTGAAACAAAAAAAAAATTTTCAACACACTTTTCTCCTCAATTTTGGCTTGCACCCGAAAAACCCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                 <  2:830535/151‑1 (MQ=255)
                                                tAATCCCCCCCCCTTGAGCATGGGGAAAAATAATAAAAGCGTGCCCCTTGACACAAAAAAAAAATTTTAAACACCCTTATCTACTCGAATTTGGCTTCCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                 <  2:163250/151‑1 (MQ=255)
                                                    gCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATgg                                                                                             >  2:721796/1‑151 (MQ=255)
                                                      ccccccctTGAGCATGGGGAAAAAAAAGAAAAGCGTGCCCCTTGACACAAATAAAAAATTTCCAACCCCCTTATCTACTCAATTTTGGCTTGCACCCAAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                           <  2:711318/146‑1 (MQ=255)
                                                        cctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTCGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTgag                                                                                         >  1:1678210/1‑151 (MQ=255)
                                                          tcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                       >  2:1529700/1‑151 (MQ=255)
                                                           cctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                                                                                      >  2:909032/1‑151 (MQ=255)
                                                                accATCGGAAAAAATAATGAAAGCGTGTCCTTTACCACAAAAAAAAAATTTTCAACACCCTTATCTCCCCGAATTTGGCTTCCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                 <  2:1207647/149‑1 (MQ=255)
                                                                acaatgggaaaaaaaaaTGAAAGCGTGTCCCTTAACAAAAAAAAAAAATTTTAACCACACTTATCAACTCAATTTTGGTTTCCACCCAAAAAAACCGGGTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                 <  1:1244607/140‑1 (MQ=255)
                                                                 gaaccggaaaaaaaaaTGAACGCTTTCCCTTGGACAAGAAAAAAAATTTTCCACCACATTTATCCCCGGGAATTTGGTTTGCACCCCAAAAAACCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                 <  2:1600269/140‑1 (MQ=255)
                                                                  cagggggAAAAATAATAAAAGCGTGTCCCTTGACACAAATACAAAATTTTAAACACACTTATCTACTAAATTTTGTTTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                 <  1:1656164/145‑1 (MQ=255)
                                                                  cATGGGGAAAAATAATAAAGGCTTGTCCTTTGACACAAATACAAAATTTTCAACCCACTTATCTACTCGATTTTGGCTTGCACCCAAAAAAACCAGGACACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                 <  1:490727/149‑1 (MQ=255)
                                                                  cATCGGGAAAAAAAATGAAAGCGTGTCCCTTGACAGAAATAAAAAATTTTCAACCCCCTTATCTACCCGAATTTGGCTTGCACCCGCAAAAACCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                                 <  2:884937/149‑1 (MQ=255)
                                                                  aatggggAAAAAAAAAAAAAGCGTTTCCCTACACAAAAACAAAAAATTTAAAACACACTTATCTTCTAAAATTGGACTAACACCCAAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTa                                                                               <  1:1617574/147‑1 (MQ=255)
                                                                    tggggAAAAAAAATGAAGGCGTGTCCCTTGACACAAATAAAAAATTTTCAACCCCTTTACCTCATCAATTTTGTCTTCCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATg                                                                             <  1:153069/149‑1 (MQ=255)
                                                                    tCGGGAAAAATAATGAAAGCGTGCCCCTTGACACGAATAAAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATg                                                                             <  2:749833/151‑1 (MQ=255)
                                                                    tCGGAAAAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTAGATTTTGGCTTGCACCCGAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATg                                                                             <  1:988767/151‑1 (MQ=255)
                                                                     cggaaaaaaTAATAAAGCCGTGTCCTTTAACACGAAAACAAAATTATCAACCCACTTACCTCCTAAATTTTGCTTTCCCCCCAAAAAAACAAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                            <  1:1328611/145‑1 (MQ=255)
                                                                      gggAAAAATAATAAAGGCTTATCCCTTAACAAGAATAAAAAATTTTCAACCCCCTTTTCTAGTCAATTTTGGTTTCCTCCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                            <  1:1051011/150‑1 (MQ=255)
                                                                      gggAAAAAAAATAAAGGCTTTCCCCTTGACACAAAAACAAATTTTTAAACACCTTTCTCTTATCAAATTTGGTTTCCTCCCGAAAAACCGTGTTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                            <  2:769765/150‑1 (MQ=255)
                                                                           aaaTAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCa                                                                      >  2:1531980/1‑151 (MQ=255)
                                                                           aaaTAATGAAACGGTTTCCCTGCTCACAAATACAAAATTTTCAACCCCCTTTTCTACCGAATTTGGGCTCGCACCCGCAAAACCCGGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCa                                                                      <  2:1677450/151‑1 (MQ=255)
                                                                           aaaTAATAAAAGCGTGCCCCTAAACACGAATAAAAATTTTTCACCACTTTTTTCTCCTGAATTTTGTCTTCCCCCCGCAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGcn                                                                      <  2:456868/151‑2 (MQ=255)
                                                                              taatGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                    >  1:1164469/1‑150 (MQ=255)
                                                                               aatGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACg                                                                  >  2:1624224/1‑151 (MQ=255)
                                                                                       gCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCCCAAAAACCCGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTaa                                                           >  2:260249/1‑150 (MQ=255)
                                                                                          tttCCCTTGACCCAAAAAAAAAATTTCCACCCCCTTTATCTCCAGGATTTTGGTTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                         <  2:970002/148‑1 (MQ=255)
                                                                                          ttccccTTGACACAAAAAAAAAATTTACAACCCACTTATCTACTCAAATTTGGTTTGCACCCACAAAAACAGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                         <  1:766801/147‑1 (MQ=255)
                                                                                          tgtCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACa                                                        >  2:703975/1‑151 (MQ=255)
                                                                                          tgtCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACa                                                        >  2:335047/1‑151 (MQ=255)
                                                                                          tgccccTTAACACAAATAAAAAATTTTCAACACCTTTATCTACTCAATTTTGGCTTGCCTCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                         <  2:177252/147‑1 (MQ=255)
                                                                                           gtCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                       >  2:1239594/1‑151 (MQ=255)
                                                                                            tCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAt                                                      >  1:748523/1‑151 (MQ=255)
                                                                                                    acacAAAAAAAAAATTTTCAACACCTTTATCTACCCAATTTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATg                                               <  2:416091/151‑1 (MQ=255)
                                                                                                       caaaaaaaaaaTTTACAACACCCTTATCTCCCCAATTTTGGCTTCCACCCAAAAAACCCAGGCCCCCGCCATTAAAATAGCGATATCCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                            <  2:968318/144‑1 (MQ=255)
                                                                                                       caaaTAAAAAATTTTCAACCCCCTTATCTCCTCAATTTTGGCTTGCATCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                            <  2:1589422/149‑1 (MQ=255)
                                                                                                       caaaTAAAAAATTTCCAACACCCTTATCTACTAAATTTGGGCTTACATCCGAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATACTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                            <  1:1430696/149‑1 (MQ=255)
                                                                                                       cGAATACAAAATTTTCACCACACTTATCTCCTCGAATTTGCTTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                            <  1:1039905/151‑1 (MQ=255)
                                                                                                       aaaaaaaaaaaTTTTCAACCCCTTTTTCCTCTTGATTTTGGTTTGAATCCAAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                            <  2:1566000/144‑1 (MQ=255)
                                                                                                       aaaaaaaaaaaTTTTCAACACACTTATATCCTAGAATTTGGCTTGCACCCGAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                            <  1:1228905/144‑1 (MQ=255)
                                                                                                       aaaaaaaaaaaTTTAAAACCCCCTTATCTCCCAAATTTTGGTTTGACCCGAAAAAACCGGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                            <  1:1541442/144‑1 (MQ=255)
                                                                                                       aaaaTAAAAAATTTTCAACCCCTTTTTCTTCCCGATTTTGGCTTACCCCAGAAAAAACCGGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                            <  2:1344269/149‑1 (MQ=255)
                                                                                                        aaaaaaaaaaTTTTACAACCCATTTACCCCCTCGATTTTGGCTTGAACCCGAAAAAACCGGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                            <  2:518222/145‑1 (MQ=255)
                                                                                                        aaaTACAAAATTACCAACTCAGTTATCTACCAAATTTGGGCTGGCACCCAAAAAAACCGGGACACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                            <  1:1401642/149‑1 (MQ=255)
                                                                                                               aatttttCAACACATTTTTCTACTAGAATTTATCTTGCACCCGAAAAAACCAGGTCTCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTgta                                    <  1:692174/148‑3 (MQ=255)
                                                                                                                 aTTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATa                                  >  2:635776/1‑151 (MQ=255)
                                                                                                                    ttcaaaCACCTTTATCTACTCGATTTTGGCTTCCATCCGAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                <  1:1464387/147‑1 (MQ=255)
                                                                                                                           ccacTTATCACCTCAATTTTGGTTTCCACCCAAAAAAACGAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTa                         <  1:533192/150‑1 (MQ=255)
                                                                                                                            cacTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                        >  1:1701568/1‑151 (MQ=255)
                                                                                                                            cacTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                        >  1:974338/1‑151 (MQ=255)
                                                                                                                            cacTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                        >  2:797692/1‑151 (MQ=255)
                                                                                                                              tttATCTACTCAATTTTCGCTTGCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                      <  1:1373624/150‑1 (MQ=255)
                                                                                                                              cTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                      >  2:1374267/1‑151 (MQ=255)
                                                                                                                              cTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                      >  2:1278758/1‑151 (MQ=255)
                                                                                                                              cTTAACTACTAGAATTTGGCTTGCATCCGCAAAAACCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTACGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGGAGTGATTGCATAGTTGAATTAatt                      >  1:1574811/1‑150 (MQ=255)
                                                                                                                               ttATCTCCTCAATTTTGGCTTGCACCCCAAAAAACCGGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                     <  2:1021184/151‑1 (MQ=255)
                                                                                                                               ttATCTCCTCAATTTGGGTTTCCATCCCAAAAAACAAGCTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                     <  2:47500/151‑1 (MQ=255)
                                                                                                                               ttATCTACTCGATTTTGCCTTGCACCCACAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                     <  1:1623416/151‑1 (MQ=255)
                                                                                                                               ttATCTACTCAATTTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                     <  2:1516999/151‑1 (MQ=255)
                                                                                                                               ttATCTACTCAAATTTGGTTTCCATCCACAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                     <  1:1455728/151‑1 (MQ=255)
                                                                                                                               ttATCTACAAAATTTTGGCTTCCACCCGCAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                     <  1:321185/151‑1 (MQ=255)
                                                                                                                                tATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATAtt                    >  2:1480742/1‑151 (MQ=255)
                                                                                                                                    tcctaaattttGGCTTGCTCCCGCAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                <  2:838732/143‑1 (MQ=255)
                                                                                                                                    tactaaaaTTTGGTTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                <  1:1206360/145‑1 (MQ=255)
                                                                                                                                    tACTCGAATTTGGCTTCCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                <  2:1441888/151‑1 (MQ=255)
                                                                                                                                     tactcaaTTTGGCTTTCCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                <  1:564106/146‑1 (MQ=255)
                                                                                                                                          attttGCCTTCCACCCGAAAAACCCGGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat          <  2:847414/149‑1 (MQ=255)
                                                                                                                                          aaTTTTGCTTTGCATCCAAAAAAACCAGGCAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACa           <  1:1451158/151‑1 (MQ=255)
                                                                                                                                          aaTTTGGCTTTCCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACa           <  1:903724/151‑1 (MQ=255)
                                                                                                                                          aaTTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat          >  1:1554890/1‑151 (MQ=255)
                                                                                                                                          aaTTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat          >  2:1051194/1‑151 (MQ=255)
                                                                                                                                                 gCTTGCCTCCGAAAAAACCGGGTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc     <  2:1523661/150‑1 (MQ=255)
                                                                                                                                                 gCTTGCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc     <  2:823062/150‑1 (MQ=255)
                                                                                                                                                  tcTTGCACCCGAAAAAACCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc     <  1:797254/149‑1 (MQ=255)
                                                                                                                                                   ctttGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc     <  1:178971/148‑1 (MQ=255)
                                                                                                                                                     ttGCACCCAAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGt  <  2:1030979/151‑1 (MQ=255)
                                                                                                                                                           |                                                                                                                                                  
ACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGT  >  NC_000913/3665550‑3665843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: