Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913354,2880TG53.8% 91.5 / 124.4 78V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (8/28);  new base G (42/0);  total (50/28)
Fisher's exact test for biased strand distribution p-value = 2.48e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.78e-14

ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGG  >  NC_000913/354138‑354434
                                                                                                                                                       |                                                                                                                                                    
aCTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGt                                                                                                                                                      <  2:623017/151‑1 (MQ=255)
      gCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAg                                                                                                                                                 <  2:275050/150‑1 (MQ=255)
          ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATgcg                                                                                                                                            >  2:1201024/1‑151 (MQ=255)
                   tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAggg                                                                                                                                  <  1:1148816/151‑1 (MQ=255)
                      cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGTAAGAATCGCTgcacagg                                                                                                                                  >  1:1247811/1‑142 (MQ=255)
                      cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCCGAAAGGGGAg                                                                                                                               >  1:358603/1‑151 (MQ=255)
                      cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAGATGGGCTGAAAGGGCAg                                                                                                                               >  2:924502/1‑151 (MQ=255)
                       ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAAAGGGCTGAAAGGGCggg                                                                                                                              >  2:1476233/1‑151 (MQ=255)
                        gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAggg                                                                                                                             >  2:1227959/1‑150 (MQ=255)
                          tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTTGGGGGAAAGATGCGCTGAAAGGGCCggggg                                                                                                                           >  2:1567580/1‑151 (MQ=255)
                          tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtgg                                                                                                                           <  2:48433/151‑1 (MQ=255)
                             cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTTGGGGGGAAGATGCGCTGTAAAGGCCgggggggg                                                                                                                        >  2:372041/1‑151 (MQ=255)
                              aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAAATGCGCTGGAAGGGGAggggggggg                                                                                                                       >  2:953544/1‑150 (MQ=255)
                              aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCTGGAAGGGGAggggggggg                                                                                                                       >  1:606428/1‑150 (MQ=255)
                               cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAgggggcgggg                                                                                                                      >  2:1119738/1‑149 (MQ=255)
                               cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAGATGGGCTGAAAGGGGAgggggggggg                                                                                                                      >  2:546273/1‑149 (MQ=255)
                               cTTTATTCCCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAAAGGGCAGAAAGGGccaggggggggg                                                                                                                      >  2:1678951/1‑140 (MQ=255)
                               cTTTATTATCGGGCGAACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTACAGAAATCCGGGCGTTGCCGAAGTGGCGGGGGGGGGGGGGGAAAACGGGCGGAAAggggggggggggggg                                                                                                                      >  1:450083/1‑139 (MQ=255)
                                        cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTc                                                                                                            <  1:247180/151‑1 (MQ=255)
                                          ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCa                                                                                                           <  1:1541517/150‑1 (MQ=255)
                                                   aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAag                                                                                                  <  2:772909/150‑1 (MQ=255)
                                                    cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGCGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGATAATGCGCCGAAAGGGCGGGCGGTGGCGGCGGTTGTCCTTCCGCAagaa                                                                                                >  1:1077998/1‑150 (MQ=255)
                                                    cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGATGGGCCGAAAGGGCAGGGGGGGGGGGGGTTTGTTATTTCGAAagag                                                                                                >  2:1310654/1‑151 (MQ=255)
                                                      aTGTAATTAACGTTGCTGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTTGTTGGGGGGAAAGTTTCGCCGCAAGGGGGCGGGGCGGGGGGGTTTGTCATTCCGCAaaagag                                                                                              >  1:1683102/1‑151 (MQ=255)
                                                        gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGAATGGCTGGAAGGGGCGGGGGGGGGGGGGGTTGGTCTTTCGAAAagagggg                                                                                             >  1:618089/1‑148 (MQ=255)
                                                        gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGGGCTGGAAGGGGAGGGGGGGGGGGGGGTTGTCATTTCGGAAAAGAGCg                                                                                            >  2:565503/1‑151 (MQ=255)
                                                        gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATTGGCTGAAAGGGGCGGGGGGGGGGGGGGTTGTCATTTCGAAagagaggg                                                                                            >  1:200141/1‑149 (MQ=255)
                                                        gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGCGCTGGAAGGGGCGGGGGCGGGGGGGGTTGTTATTTCGCAAAAGAGCg                                                                                            >  1:1457550/1‑151 (MQ=255)
                                                         tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAAGGGAGGGGGGGGGGGGGGTTGTCATTTCGAAAGAGAGGGa                                                                                           >  1:715735/1‑151 (MQ=255)
                                                         tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAGATGGGCTGAAAGGGGCGGGGGGGGGGGGGGTTTTCATTCCGGAagaggggga                                                                                           >  2:848244/1‑146 (MQ=255)
                                                         tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCTGGAAGGGGCGGGGGCGGTGGGGGTTGTCATTTCCAAAGAGAGCGa                                                                                           >  1:134709/1‑151 (MQ=255)
                                                          aaTTAGCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTGCAGCCAGCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                                                                                          <  1:1672301/151‑1 (MQ=255)
                                                          aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGAc                                                                                          <  2:1135625/151‑1 (MQ=255)
                                                          aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCCGTAAGGGGAGGGGGCGGGGGGGGTTGTCATTCCGCAAGAGAGCGa                                                                                           >  1:1605730/1‑150 (MQ=255)
                                                          aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAATGGCTGGGAGGGCAGGGGGGGGGGGGGGTTGTGAttttggcaagaaaggca                                                                                          >  1:1159140/1‑136 (MQ=255)
                                                          aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGTTGGGGGGGAAGATGGCCTGAAAGGGGCGGGGGTGGGGGCGTTTGGCATTTCGGAAGAGAGCGaa                                                                                          >  2:1218620/1‑150 (MQ=255)
                                                             tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAAATGCGCTGAAAGGGCAGGGGGGGGGGGGGGTTTTCATTTCGAAAGAGAGGGACAgg                                                                                       >  2:89963/1‑150 (MQ=255)
                                                               aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGTAAAATGCCGTGGAACGGGAGGGGGCGGGGGCGGTTTTTCTTTCCCaaaataggggaaggc                                                                                       >  2:266841/1‑138 (MQ=255)
                                                                   tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                                                                                 <  1:1227366/151‑1 (MQ=255)
                                                                     ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                <  1:976815/150‑1 (MQ=255)
                                                                     ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                <  2:828186/150‑1 (MQ=255)
                                                                     ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                <  1:1610675/150‑1 (MQ=255)
                                                                     ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                <  1:115855/150‑1 (MQ=255)
                                                                     ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCCCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                <  1:1545774/150‑1 (MQ=255)
                                                                      cGGGCATCGGCTGGGGACGCGCGAGGTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                                                                              <  2:39301/151‑1 (MQ=255)
                                                                      cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                                                                              <  2:618357/151‑1 (MQ=255)
                                                                            tCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGGGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAt                                                                        <  2:465179/151‑1 (MQ=255)
                                                                              ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAACGTGAtgt                                                                      >  2:1258128/1‑151 (MQ=255)
                                                                              ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCGGGAAGGGGAGGGGGGGGGGGCGGTTGTCATTCCGAAAGAGAGGGACAGTCTGGGAGATCGTGgtgt                                                                      >  1:591941/1‑151 (MQ=255)
                                                                                             agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCTGGAAGGGGCGGTGGGGGGGGCGTTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGCGGACTCGCCaga                                                       >  1:1699753/1‑151 (MQ=255)
                                                                                               aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                      <  2:941442/150‑1 (MQ=255)
                                                                                               aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga                                                     <  1:344851/151‑1 (MQ=255)
                                                                                                  gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACCCGCCAGAGAAgg                                                  >  2:984852/1‑151 (MQ=255)
                                                                                                  gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGGGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTTATTCCGGAAGAGAGGGACAGGCTGGAAGAACGGGATGTGGGGCACTCGCAAGaggagg                                                  >  2:641387/1‑151 (MQ=255)
                                                                                                       gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGAAGCGCTGAAAGGGCAGGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGGGCGCTCGCCAGAGAAGGCGttt                                             >  2:1153237/1‑151 (MQ=255)
                                                                                                       gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGTGGCGGTGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGGGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                             >  1:447128/1‑151 (MQ=255)
                                                                                                         gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAGATTCGCTGGAAGGGGAGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGGGCACTCCCCAGAGAAGGCGATTAt                                           >  1:846120/1‑151 (MQ=255)
                                                                                                         gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGAAGCGCTGAAAGGGAAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGGGACAGTCTGGAAGAACGGGGTGGGGCGCACTCGCAAGAGAAGGGGATTAt                                           >  2:1257301/1‑151 (MQ=255)
                                                                                                          tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTAt                                           <  1:947273/150‑1 (MQ=255)
                                                                                                           aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGTGGAAAAAGGGCCTGAAAGGCCGGGGGGGGGGGGGGTTGGCATTTCCAAAGAGAGCGCCAGTCTGGGAGATCTCTGTGGGGCGCACTCCCAAGAGAAGGCgggtgtgg                                         >  1:877241/1‑144 (MQ=255)
                                                                                                           aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGGGCACTCGCAAGAGAAGGGGATTATgg                                         >  2:209441/1‑151 (MQ=255)
                                                                                                           aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGGTGGGGGGGAAGATGCGCCGAAAGGGGAGGTGGGGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAATCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGGAGGGGATTATgg                                         >  1:1316825/1‑151 (MQ=255)
                                                                                                                    cATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCGCTGGAAGGGGGGGGGGGGGGGGCGTTTGTCATTTCGGAAGAGAGCGACAGGTTGGAAGACCGTGATGTGGGGCACTCGCAAGAGAAGGGGAGTTTGGCGCtggtgg                                >  1:1116150/1‑151 (MQ=255)
                                                                                                                      tCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGAc                              <  2:869214/151‑1 (MQ=255)
                                                                                                                              ttGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAt                      <  2:1670632/151‑1 (MQ=255)
                                                                                                                               tGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCTGGAAGGGGCGGGGGGGGGGGGGGTTGTCCTTTCCAAAGAGAGCGGCAGGCTGGGAGATCGTGATGGTGCGCACTCGCAAGAGAAGGCGGTTATGGGGCTGGGGGACAGCCAGAtt                     >  1:106216/1‑151 (MQ=255)
                                                                                                                                 ccGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGTGCTGGTGGACAGCCAGATTg                    <  2:47973/150‑1 (MQ=255)
                                                                                                                                 ccGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTg                    <  1:565274/150‑1 (MQ=255)
                                                                                                                                  cGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGc                  <  1:1531118/151‑1 (MQ=255)
                                                                                                                                    aaGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGTTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGGTTTTGGGGCTGGGGGGCAGCCCGATTGGCaa                >  2:1175293/1‑151 (MQ=255)
                                                                                                                                       tGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAAc               <  2:608781/149‑1 (MQ=255)
                                                                                                                                       tGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACtt             <  1:622687/151‑1 (MQ=255)
                                                                                                                                        ggCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACttt            >  1:1610414/1‑151 (MQ=255)
                                                                                                                                         gCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg           >  2:477803/1‑151 (MQ=255)
                                                                                                                                         gCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg           >  2:252030/1‑151 (MQ=255)
                                                                                                                                         gCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg           >  1:414156/1‑151 (MQ=255)
                                                                                                                                             tggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg       >  1:1515428/1‑151 (MQ=255)
                                                                                                                                                 ttGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGccc    <  2:976772/151‑1 (MQ=255)
                                                                                                                                                    gggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg  >  2:853349/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                    
ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGG  >  NC_000913/354138‑354434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: