Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	540,558	0	T	G	45.6%	104.5 / 44.3	92	intergenic (+50/‑7)	allB/ybbY	allantoinase/putative uracil/xanthine transporter
Reads supporting (aligned to +/- strand):  ref base T (15/35);  new base G (42/0);  total (57/35)
Fisher's exact test for biased strand distribution p-value = 9.44e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACTTTCTGCTTTTCAGTTGCCGCAAAGTAGCCTGCTTACGCTCACGC  >  NC_000913/540409‑540706                                                                                                                                                      |                                                                                                                                                     tATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGgtg                                                                                                                                                     <  1:478265/151‑1 (MQ=255) tATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGgtg                                                                                                                                                     <  1:645891/151‑1 (MQ=255) tATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGgtg                                                                                                                                                     <  2:1322048/151‑1 (MQ=255) tATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGgtg                                                                                                                                                     <  2:1732870/151‑1 (MQ=255)      cGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTTAGGGGGGttt                                                                                                                                                >  2:1534496/1‑151 (MQ=255)        aaaaCCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTa                                                                                                                                               <  1:1084218/150‑1 (MQ=255)        aaaaCCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTa                                                                                                                                               <  2:372911/150‑1 (MQ=255)         aaaCCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATg                                                                                                                                             <  2:1084508/151‑1 (MQ=255)           aCCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGtt                                                                                                                                           <  2:111885/151‑1 (MQ=255)            ccATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTc                                                                                                                                          <  1:845768/151‑1 (MQ=255)            ccATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTc                                                                                                                                          <  1:1561328/151‑1 (MQ=255)              aTCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGGTTAGGGGTGTTTTTGTTcta                                                                                                                                        >  1:39397/1‑149 (MQ=255)              aTCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGTGTTTTTGTTCaa                                                                                                                                        >  2:1475013/1‑151 (MQ=255)               tCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGTTTAGGGGTGTTTATGTTTAAt                                                                                                                                       >  2:1546716/1‑151 (MQ=255)                cTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAAtt                                                                                                                                      <  2:103197/151‑1 (MQ=255)                      tggtgATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAg                                                                                                                                <  2:1134896/151‑1 (MQ=255)                          gatgtgatTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGGATTCTCCGGTTAAGGGGTGGTTTTGTTCCATTTTgcaggcag                                                                                                                            >  2:550643/1‑151 (MQ=255)                          gatgtgatTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGTGTTTATGTTCAATTTTtcagtcag                                                                                                                            >  1:526990/1‑151 (MQ=255)                                atTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGa                                                                                                                      <  2:184393/151‑1 (MQ=255)                                atTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTAAGGGGTGGTTATGTTCAATTTTTCAGTCAGCCGCGa                                                                                                                      >  1:1630070/1‑151 (MQ=255)                                  ttACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGGTAAGGGGTGGTTTTGTTCAATTTTGCAGGCCGCCGCGgaa                                                                                                                    >  2:93735/1‑151 (MQ=255)                                  ttACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTTAGGTGTGTTTTTGTTCAATTTTTCAGTCAGCCCCGcaa                                                                                                                    >  1:1315970/1‑151 (MQ=255)                                   tACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAg                                                                                                                   <  1:117981/151‑1 (MQ=255)                                       aCATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGTTAAGGGGTGTTTTTGTTCAATTTTTCAGGCAGCCGCGCAAGc                                                                                                                  >  1:1270999/1‑148 (MQ=255)                                       aCATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGTGTTTTTGTTCAATTTTTCCGTCAGCCGCGAAAGc                                                                                                                  >  2:972992/1‑148 (MQ=255)                                       aCATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTAAGGGGTGTTTTTGTTTAATTTTTCAGTCAGCCGCGAAAGc                                                                                                                  >  2:1721194/1‑148 (MQ=255)                                       aCATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTAAGGGGTGTTTTTGTTCAATTTTTCAGTCAGCCGCGAAAGc                                                                                                                  >  2:665420/1‑148 (MQ=255)                                        cATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTTAGGGGGGTTTTTGTTCAATTTTTCAGTCAGCCCCGAAAGc                                                                                                                  >  2:478609/1‑147 (MQ=255)                                         aTTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTAAGGGGGGTTTTTGTTTCATTTTTCCGTCCGCCCCGAAAGCCTGtt                                                                                                             >  2:1688291/1‑151 (MQ=255)                                          ttGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTa                                                                                                            <  2:245285/151‑1 (MQ=255)                                            gAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGTTAAGGGGGGTTTTTGTTTCATTTTTCAGTCAGCCGCGAAAGCCCGtttt                                                                                                           >  2:216548/1‑148 (MQ=255)                                            gAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTTAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCCCGAAAGCCTGtttt                                                                                                           >  2:1142349/1‑148 (MQ=255)                                            gAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGTGTTTTTGTTCAATTTTTCAGTCAGCCGCGCAAGCCTGTTAtt                                                                                                          >  1:1256654/1‑150 (MQ=255)                                            gAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTAt                                                                                                           >  2:174740/1‑150 (MQ=255)                                            gAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGGGGTTTTGTTTAATTTTGCCGTCAGCCGCGAAAGCCTGTTAt                                                                                                           >  2:820801/1‑150 (MQ=255)                                            gAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTTAGGGGTGTTTTTGTTTAATTTTTCAGTCAGCCGCGAAAGCCCGtttt                                                                                                           >  2:111734/1‑148 (MQ=255)                                            gAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGAATTCTCCGGTTAAGGGGTGTTTATGTTTAATTTTTCTGTCCGCCGGGAAAGGCTGtttt                                                                                                           >  2:1615070/1‑148 (MQ=255)                                            gAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGCCCTTGCGGCGGGCATTCTCCGGTTAAGGGGTGTTTTTGTTTAATTTTTCCGTCAGCCGCGAAAGCCTGtttt                                                                                                           >  2:341335/1‑148 (MQ=255)                                                 gggCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGGGTTTTTGTTTAATTTTTTAGTCCGCCGCGAAAGCCTGtttttcgggt                                                                                                     >  2:670703/1‑143 (MQ=255)                                                  ggCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGTTTAGGGGTGTTTTTGTTTAATTTTGTAGGCAGCCGCGAAAGCCTGTTTTCAGGGtt                                                                                                    >  1:315358/1‑151 (MQ=255)                                                  ggCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAgg                                                                                                       <  1:328743/148‑1 (MQ=255)                                                    cTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTAAGGTGGGTTTATGTTCAATTTTGCAGGCAGCCGCGAAAGCCTGTTTTCAGGGtttt                                                                                                  >  2:225976/1‑150 (MQ=255)                                                      tCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGGGGTTTTGTTCAATTTTTCAGTCAGCCGCGAAAGCCTGTTTTTAGGATTTTAg                                                                                                >  2:1291804/1‑151 (MQ=255)                                                           gTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTTTTCGGGTTAGGGGTGttt                                                                                                                                                >  1:1676401/1‑98 (MQ=255)                                                           gTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGttt                                                                                                                                                <  2:1677249/98‑1 (MQ=255)                                                                       ggTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGTGTTTTTTTTCAATTTTTCAGTCAGCCGCGCAAGCCTGTTATCAGGGTTTCAGTGGtttttttttttttt                                                                               >  2:1383000/1‑151 (MQ=255)                                                                       ggTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGTGTTTTTGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTTAGTGGTTTTTCTTTAtttt                                                                               >  1:1608746/1‑151 (MQ=255)                                                                        gTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGTGTTTTTGTTTAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGGTTTTTGTGGGtttttttttttttt                                                                              >  2:491275/1‑151 (MQ=255)                                                                          cAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCCTTTTCCGGTTTAGGTGTGTTTTTGTTCAATTTTGCAGGCAGCCGCGAAAGCCCGTTTTTAGGGTTTCCGTGGGTttttttttttttttt                                                                            >  2:903666/1‑150 (MQ=255)                                                                           aaTTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGc                                                                           <  2:829788/151‑1 (MQ=255)                                                                            aTTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGTTAAGGGGTGTTTTTGTTTAATTTTGCCGTCAGCCGCGAAAGCCTGTTATCAGGATTTTAGTGGGTTTTTTTTTTTTTTTTCa                                                                          >  2:1285840/1‑151 (MQ=255)                                                                            aTTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCCGGTTAGGGGTGTTTATGTTCAATTTTTCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTTAGGGGTTTTTCTTTATTTTTTTCa                                                                          >  1:1428180/1‑151 (MQ=255)                                                                                aTCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAacac                                                                      <  1:1609856/151‑1 (MQ=255)                                                                                 tCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCAATCTCCGGGTAAGGTGTGGTTTTTTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTTTTAGGGTTTTAGTGGGTTTTTTTTATTTTTTGCAACACg                                                                     >  1:823677/1‑151 (MQ=255)                                                                                  ccTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGTGTTTTTGTTTAATTTTTCCGTCAGCCGCGGAAGCCTGTTATCAGGGTTTCAGTGGGTTTTCTTTTTTTTTTGCAACACgg                                                                    >  2:1617743/1‑151 (MQ=255)                                                                                   cTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCCGGTTAGGTGGGTTTATGTTCACTTTTGCAGTTAGCCGCGAAAGCCTGTTATTTGGCTTTCAGTGGTTTTTTTTTATTTTTTTCAACACggg                                                                   >  1:570062/1‑150 (MQ=255)                                                                                        aCATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTgg                                                              <  2:483611/151‑1 (MQ=255)                                                                                        aCATCAGCAGTAATCTGGCCCCTGCAATGCCCGCCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTgg                                                              <  1:341223/151‑1 (MQ=255)                                                                                         cATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGGATTTTCCCGGTAAGGGGGGTTTTTGTTGGATTTTTGAGTCAGCCCGGAAACCCTGTTTTCAGTGGTTCTGGTGGTTTTCTTTTTTTTTTTTAACACGGTTGTGGt                                                             >  2:54584/1‑151 (MQ=255)                                                                                         cATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGt                                                             <  2:1037669/151‑1 (MQ=255)                                                                                         cATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGTGTTTTTGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGGTTTTAGTGGGTTTTCTTTATTTTTTGCAACACGGTTGTggg                                                             >  1:1102854/1‑150 (MQ=255)                                                                                          aTCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGtt                                                            <  2:1085318/151‑1 (MQ=255)                                                                                          aTCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGtt                                                            <  1:1023703/151‑1 (MQ=255)                                                                                              gcagTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGTTAAGGGGTGTTTATGTTCCATTTTTCAGTCAGCCCCGAAAGCCCGTTATCAGGGTTTCTGTGGTTTTTTTTTTTTTTTTTTAACACGGTTTTGGTtcct                                                         >  2:470539/1‑150 (MQ=255)                                                                                              gcagTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTTAGGGGTGTTTTTGTTTAATTTTTCAGTCAGCCGCGAAAGCCTGTTATCAGGGTTTCAGTGGGTTTTCTTTATTTTTTGCAAAACGGTTGTgggttct                                                         >  1:356774/1‑145 (MQ=255)                                                                                              gcagTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTAAGGGGTGGTTATGTTCAATTTTTCAGGCCGCCGCGAAAGCCTTTTATCAGGGTTTCAGGGGGTTTTTTTTTTTTTTTTCAACACGGTTGTGGTttct                                                         >  2:1668200/1‑150 (MQ=255)                                                                                              gcagTAATCTGGCCCCTGCAATGCCCGTCCTTGCCGCGGGTGTTCTTCGGGTAAGGGGTTGTTATTGTCAATTTTGCCGGCAACCGCGAAACCCTGTTATTAGGATTTCTGTTGTTTTTTTTTTTTTTTTTCCAAACGGGTTTGGAtcct                                                         >  2:347263/1‑150 (MQ=255)                                                                                               cagTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTtcctc                                                        <  1:365418/150‑1 (MQ=255)                                                                                               cagTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTtcct                                                         <  2:1738519/149‑1 (MQ=255)                                                                                                  tAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTtcctcc                                                       <  2:545998/148‑1 (MQ=255)                                                                                                  tAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTtcctcc                                                       <  2:1630887/148‑1 (MQ=255)                                                                                                   aaTCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGGATTCTCCGGGTAAGGTGTGTTTTTTTTCGATTTTTCCGGCCCCCCCGCAAGCCTGTTATTAGGATTTCAGTGGGTTTTTTTTTTTTTTTGTAACACGGGTGGGGTTCCTCCCAc                                                    >  2:791908/1‑150 (MQ=255)                                                                                                   aaTCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCCTTCTCCGGTTAAGGTGGGTTTTTGTTCAATTTTTCAGTCAGCCGCGAAAGCCTGTTTTTAGGGTTTCAGTGGTTTTTTTTTTTTTTTTTCAAAACGGGTTTGGGTCCTCCTAc                                                    >  2:1035962/1‑150 (MQ=255)                                                                                                   aaTCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTTAGGGGTGTTTTTGTTTCATTTTTCCGTTAGCCGCGAAAGACCGTTTTCCGGATTTCCGTGGTTTTTCTTTTTTTTTTGCAACACGGTTGTGGGTCCTCCTAc                                                    >  1:1438628/1‑150 (MQ=255)                                                                                                   aaTCTGGCCCCTGCAATACCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGGGTTTTTGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTTTCAGGATTTCAGTGGTTTTTTTTTTTTTTTTTCAACACGGTTGTGGTTCCTCCTACg                                                   >  2:145568/1‑151 (MQ=255)                                                                                                    aTCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGc                                                  <  1:135035/151‑1 (MQ=255)                                                                                                    aTCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGc                                                  <  2:1103413/151‑1 (MQ=255)                                                                                                     tCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTAAGGGGTGTTTTTGTTCAATTTTTCAGTCAGCCGCGAAAGCCTGTTTTCAGGATTTTCGTGGGTTTTTTTTTTTTTTTTCTAACCGGTTGTGGTTTCCCCTTCg                                                   >  2:1624105/1‑149 (MQ=255)                                                                                                          ccccTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACtt                                             <  2:1308575/150‑1 (MQ=255)                                                                                                          ccccTGCAATGCCCGTCCTTGCGGCGGGCATGCGCCGGTTAAGGTGTGTTTATGGTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGGTGTGGTTCCTCCTACGCTACtg                                             <  1:112424/150‑2 (MQ=255)                                                                                                           cccTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACTTTc                                           <  1:280178/151‑1 (MQ=255)                                                                                                           cccTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGTGTTTATGTTCAATTTTTCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGGTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACttt                                            >  1:622402/1‑150 (MQ=255)                                                                                                              tGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTAAGGTGTGTTTTTGTTCAATTTTTCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTTTTTTTTTCAACACGGTTTTTGTTCCTCCCACGCTACttttttt                                        >  1:195915/1‑147 (MQ=255)                                                                                                                   tGCCCGTCCTTGCGGCGGGCATTCTCCGGGTTAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGGAAGCCTGTTATCCGGGTTTTAGTGGTTTTTTTTTTTTTTTTTCAACACCGTTGTGGTTTCTCCTACGCTACTTTCTGCttttt                                   >  1:1463739/1‑150 (MQ=255)                                                                                                                     cccGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACTTTCTGCTTTTCAg                                 <  2:1257260/151‑1 (MQ=255)                                                                                                                         tCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTTAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTTTTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACTTTTTGCTTTTTAGTTg                              >  2:223876/1‑150 (MQ=255)                                                                                                                         tCCTTGCGGCGGGCATTCTCCGGTTAAGGGGTGTTTATGTTCAATTTTTCAGTCAGCCGCGAAAGCCTGTTATCAGGGTTTCAGTGGGTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACTTTCTTCTTTTCAGTTg                              >  1:1428401/1‑150 (MQ=255)                                                                                                                                 gcggGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACTTTCTGCTTTTCAGTTGCCGCCAAGt                     >  1:1337388/1‑151 (MQ=255)                                                                                                                                  cggGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCCGTCAGCCGCGAAAAGCTGTTATCCGGATTTCAGTGGGTTTTCTTTATTTTTTGCAACCCGGTTGTGGTTCCTCCTACGCTACTTTCTGCTTTTCAGTTGCCGCAAAGt                     <  1:79272/150‑1 (MQ=255)                                                                                                                                  cggGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACTTTCTGCTTTTCAGTTGCCGCAAAGt                     <  2:1076587/150‑1 (MQ=255)                                                                                                                                   gggCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACTTTCTGCTTTTCAGTTGCCGCAAAGTAg                   >  2:1316785/1‑151 (MQ=255)                                                                                                                                     gCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACTTTCTGCTTTTCAGTTGCCGCAAAGTAGc                  <  2:414775/150‑1 (MQ=255)                                                                                                                                      cATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACTTTCTGCTTTTCAGTTGCCGCAAAGTAGcc                 <  1:267522/150‑1 (MQ=255)                                                                                                                                      cATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACTTTCTGCTTTTCAGTTGCCGCAAAGTAGcc                 <  1:1031860/150‑1 (MQ=255)                                                                                                                                      cATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACTTTCTGCTTTTCAGTTGCCGCAAAGTAGc                  >  1:346698/1‑149 (MQ=255)                                                                                                                                               gTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACTTTCTGCTTTTCAGTTGCCGCAAAGTAGCCTGCTTACg         >  2:1596459/1‑149 (MQ=255)                                                                                                                                                     gtgtgtTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACTTTCTGCTTTTCAGTTGCCGCAAAGTAGCCTGCTTACGCt       <  1:1157212/145‑1 (MQ=255)                                                                                                                                                     gtgtgtTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACTTTCTGCTTTTCAGTTGCCGCAAAGTAGCCTGCTTACGCt       >  2:1157782/1‑145 (MQ=255)                                                                                                                                                     gtgtgtTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACTTTCTGCTTTTCAGTTGCCGCAAAGTAGCCTGCTTACGCTCACGc  <  2:527210/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     TATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGTGGTTCCTCCTACGCTACTTTCTGCTTTTCAGTTGCCGCAAAGTAGCCTGCTTACGCTCACGC  >  NC_000913/540409‑540706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑