Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913657,6760AC48.2% 147.8 / 40.4 115V88G (GTG→GGG) flcfluoride efflux channel, dual topology membrane protein
Reads supporting (aligned to +/- strand):  ref base A (36/23);  new base C (0/55);  total (36/79)
Fisher's exact test for biased strand distribution p-value = 2.43e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

CGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTG  >  NC_000913/657527‑657823
                                                                                                                                                     |                                                                                                                                                   
cGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAacac                                                                                                                                                    <  1:1709022/151‑1 (MQ=255)
cGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAacac                                                                                                                                                    <  2:1221875/151‑1 (MQ=255)
cGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAaca                                                                                                                                                     >  1:363531/1‑150 (MQ=255)
      aTCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTc                                                                                                                                              <  1:1730102/151‑1 (MQ=255)
           cGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCg                                                                                                                                         <  2:802847/151‑1 (MQ=255)
             ggTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGaa                                                                                                                                       >  2:1033101/1‑151 (MQ=255)
             ggTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGaa                                                                                                                                       >  2:1114121/1‑151 (MQ=255)
               ttttttttGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGaaaa                                                                                                                                     >  1:615024/1‑151 (MQ=255)
                tttttttGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAAt                                                                                                                                    >  2:865007/1‑151 (MQ=255)
                  tttttGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGt                                                                                                                                  >  1:1344778/1‑151 (MQ=255)
                      tGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTgag                                                                                                                              <  2:598309/151‑1 (MQ=255)
                       ggTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTgaga                                                                                                                             >  2:1295187/1‑151 (MQ=255)
                        gTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTgaga                                                                                                                             <  1:1158016/150‑1 (MQ=255)
                        gTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTgaga                                                                                                                             <  2:738514/150‑1 (MQ=255)
                        gTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGaa                                                                                                                            >  2:260873/1‑151 (MQ=255)
                          cTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAgg                                                                                                                          <  1:405714/151‑1 (MQ=255)
                            ttAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGgtt                                                                                                                        >  2:650711/1‑151 (MQ=255)
                              aGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGgttgt                                                                                                                      >  1:403496/1‑151 (MQ=255)
                               gtgtGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGgttgtt                                                                                                                     >  2:338713/1‑151 (MQ=255)
                                 gtgCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAg                                                                                                                   >  1:720778/1‑151 (MQ=255)
                                 gtgCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAg                                                                                                                   >  1:1614587/1‑151 (MQ=255)
                                 gtgCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAg                                                                                                                   >  1:718019/1‑151 (MQ=255)
                                 gtgCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAg                                                                                                                   >  2:1728242/1‑151 (MQ=255)
                                  tgCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAg                                                                                                                   <  1:467354/150‑1 (MQ=255)
                                  tgCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAg                                                                                                                   <  2:916790/150‑1 (MQ=255)
                                    cGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCTGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGAc                                                                                                                 <  2:510265/150‑1 (MQ=255)
                                    cGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGAcc                                                                                                                <  2:1590127/151‑1 (MQ=255)
                                      gTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCg                                                                                                               >  2:1684823/1‑150 (MQ=255)
                                            gCCAAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATCCCCAGCAAAAGCGGCCCTCTGGTAACAAAAACCCCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACaa                                                                                                         <  1:1087864/150‑1 (MQ=255)
                                                aaaaCAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATc                                                                                                     >  1:1193920/1‑150 (MQ=255)
                                                      gCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCggtggt                                                                                              >  2:133996/1‑151 (MQ=255)
                                                        cAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGa                                                                                            >  1:456304/1‑151 (MQ=255)
                                                          gAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGAtt                                                                                          >  1:1602502/1‑151 (MQ=255)
                                                             tGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTaaa                                                                                       >  2:1371958/1‑150 (MQ=255)
                                                              gCCGGTGCGGTCAGGGAAAAAGCCCCCAGAAGGTTACCAAAACCTTTCAGAAATGCCCAGCAAAAGGGGCCTTTTTGAACCAAAACCCCCCCTTCGGCGAAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAAt                                                                                       <  1:1132902/150‑1 (MQ=255)
                                                               ccggTGCGGTAATGGCAAAAGACCCCAAAAGGTTGACAAAAACGTTCAGTAATGCCCAGCAAAAGCGGCCCTCTTGTAACAAAAACCCCCCTTCTGCCAAAAATTTTGAAAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATAc                                                                                     <  2:1594849/148‑1 (MQ=255)
                                                               ccAGTGCGGTCATGGAAAAAGCCCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAAAAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATAc                                                                                     <  2:288565/151‑1 (MQ=255)
                                                               ccAGTGCGGTCAGGGAAAAAGCCCCCAGAAGTTTGACAAAAACGTTCAGTAATCCCCAGCAAAAGCGGCCCTTTTGTAACAAAAACCCCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATAc                                                                                     <  2:759361/151‑1 (MQ=255)
                                                               ccAGTGCGGTAAGGGCAAAAGACCCCAGAAGGTTGACGAAACCGTTCAGTAATGCCCAGCAAAGGCGGCCCTCTTGAAACAAAAACCCCACTCCGGCCAAAAAGGTTGAGAAGGTTTTTAGACCGCCACAAAATCCGGTGGTGATTAATAc                                                                                     <  1:385275/151‑1 (MQ=255)
                                                               ccAGGGCGGTCAGGGAAAAATCCCCCAAAAGGTTAACAAAACCGTACAGTAAGGCCTAGCCAACGCGGCCGTTTTGAACCAAAAACCCCTCTTCGCGCAAAAATGTTAAGAAGTTTTTTAGCCCGCCACAAAATCCGGTGGTGATTAATAc                                                                                     <  1:1181337/151‑1 (MQ=255)
                                                                  gTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACttt                                                                                  >  2:149310/1‑151 (MQ=255)
                                                                     cGGTCATGGCAAAAGCCCCCAGAAGGTTGACAAAAACGTTCAGTAATGCCCAGCAAAAGCGGCCCTCTTGTAACAAAAACCCCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTcc                                                                                <  2:93712/150‑1 (MQ=255)
                                                                        tCATGGCAAAAGCCCCCAGAAGGTTGACAAAAACTTTCAGTAATGCCCAGCAAAGGCGGCCCTCTTGTAACAAAAACCCCCCTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCacac                                                                            <  2:1720183/151‑1 (MQ=255)
                                                                        tCATGGCAAAAGCCCCCAGAAGGTTGACAAAAACGTTCAGTAAGGCCCAGCCAAAGCGGCCCTTTTGTAAAAAAACCCCCCCTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCacac                                                                            <  2:409694/151‑1 (MQ=255)
                                                                        tCAGGGCAAAAGACCCAAGAAGGTTTACAAAACCTTTCATTAATGCCCGTCAAAAGGGGCCCTCTTTAAAAAAAAACACCACTCCCCCCAAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCacac                                                                            <  2:1247486/151‑1 (MQ=255)
                                                                        tCAGGGAAAAAGACCCCAGAAGGTTGACAAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACCCCACTTCTGCCAAAAATGTTGAGAAGGTTGTTAAACCGCCACAAAATCCGGTGGTGATTAATACTTTCCacac                                                                            <  2:247320/151‑1 (MQ=255)
                                                                        tCACGGAAAAACCCCCCGGAAGGTTAAAAAAAACTTTCAGAAATCCCCACCAAAAGCGCCCCCCTTGAAACAAAACCACCCCTCCTGCCAAAAATGTGGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCcaca                                                                             <  2:643184/150‑1 (MQ=255)
                                                                           tGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTgg                                                                         >  1:936961/1‑151 (MQ=255)
                                                                            ggaaaaaGCCCCCAGAAGGTTGACGAAACCGTTCAGTAAGCCCCACCCAAAGCGGCCCTCTTGTAAAAAAACCACCACTCCGGCGAAAAATGTTGAGAAGGTTTTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACt                                                                           <  1:925692/145‑1 (MQ=255)
                                                                             gCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGAt                                                                       >  1:334033/1‑151 (MQ=255)
                                                                             gCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGAt                                                                       >  1:293236/1‑151 (MQ=255)
                                                                                aaaGCCCCCAGAAGTTTGACGAAACCTTCCAGTAATCCCCAGCCAAGGCGGCCTTCTTTTAACAAAAACCCCCCTTCTGCCAAAAATGTTGAGAAGTTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCaa                                                                    <  1:560897/151‑1 (MQ=255)
                                                                                   gACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAGAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGt                                                                 >  1:679053/1‑151 (MQ=255)
                                                                                    cccccAGAAGGTTGACAAAACCGTTCAGTAATGCCCAGCAAAAGCGGCCCTTTTGAAACAAAAACCCCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATg                                                                  <  1:598640/148‑1 (MQ=255)
                                                                                    cccccAGAAGGTTGACAAAAACGTTCAGTAATGCCCAGCAAAAGCGGCCCTCTTATAACAAAAACCCCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGt                                                                 <  1:1423495/149‑1 (MQ=255)
                                                                                    aCCCCAGAAGGTTGACAAAAACGTTCAGTAATGCCCAGCAAAGGCGGCCCTTTTGAAAAAAAAACCCCCCTTCTGCCAAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATg                                                                  <  2:711795/149‑1 (MQ=255)
                                                                                    aCCCAAGAAGGTTGAAGAAACCGTTCAGTAATGCCCAGCCAAAGCGCCCCTCTTGAAAAAAAACCCCCACTTCTGCGAAAAATGTTGAGAGGTTTTTAAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATg                                                                  <  1:1420772/149‑1 (MQ=255)
                                                                                              gTTGACGAAAACGTTCAGTAATCCCCAGCCAAGGCGCCCCTCTTGTAACAAAAACCCCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATcc                                                        <  1:1209896/149‑1 (MQ=255)
                                                                                                    aaaacctttCATTAATGCCCACCCAAGGCGGCCCTCTTATAACAAAAACCCCACTTCTGCCGAAATTTTTGAAAAGGTTGTTAAACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAAc                                                <  1:1683967/144‑1 (MQ=255)
                                                                                                    aaaacCGTTCACAAAGGCCGGGCAAAAGCGGCCCTCTAATAACAAAAACACCCCTTCCGCGAAAAATGTTGGGAGGGTTGTTAGTCCGCCACAAAATCCGGTGGTGATTAATACTTTCCACAATGGATCAATGTTCGTCATCCTGCTGAAc                                                <  2:705130/150‑1 (MQ=255)
                                                                                                    aaaaaCTTTCAGTAATGCCCAGCCAAAGCGGCCCTTTTGAAAAAAAAACCCCCCTTCGGCCAAAAATTTTGAAAGGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAAc                                                <  2:666990/150‑1 (MQ=255)
                                                                                                    aaaaaCTTTCAGTAATGCCCACCCAAAGCGGCCCTCTTGTAAAAAAACCCCCACTTCTCCCAAAAATGTTGAAAAGGTTTTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAAc                                                <  2:1376760/150‑1 (MQ=255)
                                                                                                    aaaaaCGTTCATTAATGCCCAGCAAAAGCGGCCTTCTTTAAAAAAAACCCCCACTTCGGCCAAAAATGTTGAGAGGGTTGTCAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAAc                                                <  1:1057663/150‑1 (MQ=255)
                                                                                                    aaaaaCGTTCAGTAATCCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACCCCACTTCTGCCGAAAATTTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAAc                                                <  1:1167988/150‑1 (MQ=255)
                                                                                                    aaaaaCGTTCAGAAATGCCCAGCCAAAGCGGCCCTCTGGTAAAAAAAACCCCACTTCTGCCAAAAATGTTGAGAAGTTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAAc                                                <  2:511764/150‑1 (MQ=255)
                                                                                                    aaaaaCGTTCAGAAATGCCCAGCAAAAGCGGCCCTCTTGAAAAAAAAACCCCACTTCTGCCGAAAATTTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAAc                                                <  2:1117833/150‑1 (MQ=255)
                                                                                                      aacctttCATTAATCCCCAGCCGACGCGGCCCTTTTTTAACAAAAACCCCCCTCCTGCCGAAATTGTTGAGATGTTTTTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCa                                              <  1:1312448/146‑1 (MQ=255)
                                                                                                      aaaCGTTCAGTAATGCCCAGCAAAGGCGGCCCTCTTGTAACAAAAACCCCCCTTCTGCCAAAAATGTTGAGAAGGTTTTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCa                                              <  2:721146/151‑1 (MQ=255)
                                                                                                        aCGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATg                                            >  2:1273656/1‑151 (MQ=255)
                                                                                                          gTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCg                                          >  2:959783/1‑151 (MQ=255)
                                                                                                          gTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCg                                          >  2:651073/1‑151 (MQ=255)
                                                                                                               gTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtc                                      >  2:732805/1‑150 (MQ=255)
                                                                                                                tAATGCCCAGCCAAAGCGGCCCTCTTGTAAAAAAAACCCCACTTCGGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcc                                     <  2:830105/150‑1 (MQ=255)
                                                                                                                aaaTCCCCAGCAAAGGCGGCCTTCTTGTAACAAAAACCCCACTTCGGCAAAAAATGTTGAAAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcc                                     <  1:945992/149‑1 (MQ=255)
                                                                                                                    ccccAGCCAAAGCGGCCCTCTTGAAAAAAAAACCCCCCTTCTGCCAAAAATGTTGAAAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattc                                <  1:1682338/150‑1 (MQ=255)
                                                                                                                     cccgccaaaaGCGCCCCTTTTGAAAAAAAAACCCCCCTTCCGCCAAAAATGTTGAAAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattcc                               <  2:665920/144‑1 (MQ=255)
                                                                                                                     cccAGCCAGAGCGGCCCTTTTATACAAAAAACCCCACTCCTGCCGAAATTGTTGAGAAGTTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattc                                <  1:47971/150‑1 (MQ=255)
                                                                                                                     cccAGCCAAGGCGCCCCTTTTGAAAAAAAAACCCCACTTCTGCCAAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattcc                               <  2:1317040/151‑1 (MQ=255)
                                                                                                                     cccAGCCAAAGCGGCCCTCTTTAAAAAAAAACCCCCCTTCTGCCAAAAATGTTGAGAAGGTTTTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattc                                <  2:973460/150‑1 (MQ=255)
                                                                                                                     cccAGCCAAAGCGGCCCTCTTGTAACAAAACCCCCACTTCGGCCAAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattcc                               <  2:1136455/151‑1 (MQ=255)
                                                                                                                     cccACCAAAGGCGGCCCTCTTGAAACAAAAACCCCCCTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattc                                <  2:58511/150‑1 (MQ=255)
                                                                                                                     cccACCAAAAGCGGCCCTCTTGAAACAAAACCCCCCCTTCTCCCGAAAATTTTAAGAGGGTTGTAAAACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattcc                               <  2:635632/151‑1 (MQ=255)
                                                                                                                     cccACCAAAAGCGCCCCTTTTGAAAAAAAAACCCCCCTTCTGCCAAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattc                                <  2:403641/150‑1 (MQ=255)
                                                                                                                     ccaaGCCAAAGCGGCCCTCTTGTAACAAAAACCCCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattcc                               <  1:1087173/148‑1 (MQ=255)
                                                                                                                       cggcaaaaGCGGCCCTTTTTAAACAAAACCCCCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattccta                             <  2:1260426/146‑1 (MQ=255)
                                                                                                                       ccgcaaaaGCGGCCCTCTTGAAAAAAAACCCCCCCTTCTGCCAAAAATTTTGAGAAGGTTTTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattccta                             <  2:377932/146‑1 (MQ=255)
                                                                                                                       cacccAAGGCGGCCCTTTTGAAACAAAAACCCCCCTTCTGCCAAAAATGTTGAGAAGTTTGTTAGCCCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattccta                             <  2:363647/148‑1 (MQ=255)
                                                                                                                         gCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGCTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTatg                           >  1:606968/1‑151 (MQ=255)
                                                                                                                         cccAAAGCGGCCCTTTTGTAACAAAAACACCACTTCTGCCAAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTatg                           <  1:1523187/150‑1 (MQ=255)
                                                                                                                          caaaaGCGGCCCTTTTGTAACAAAAACCCCCCTTCTGCCGAAAATGTTGAAAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTatga                          <  2:287363/149‑1 (MQ=255)
                                                                                                                            aaggggccccTTTTGTAAAAAAAACCCCACTTCGCCCAAAAATTTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTatgatg                        <  2:733647/144‑1 (MQ=255)
                                                                                                                            aaaGCGGCCCTCTTTTAAAAAAAACCCCCCTTCTGCGGAAAATTTTGAGAAGGTTTTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTatgatg                        <  2:965832/151‑1 (MQ=255)
                                                                                                                            aaaGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTatgatg                        <  1:322599/151‑1 (MQ=255)
                                                                                                                            aaaGCGGCCCTCTTGAAACAAAAACCCCCCTTCTGCCGAAAATTTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTatgatg                        <  2:1642391/151‑1 (MQ=255)
                                                                                                                            aaaGCGCCCCTCTTGAACCAAAACCCCCCCTTCCGCCAAAAATGTTGAGAAGTTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTatgatg                        <  2:1383736/151‑1 (MQ=255)
                                                                                                                                   cccTCTTGAAAAAAAAACCCCACTTCCGCCAAAAATGTTGAGAAGGTTTTTAAACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGcc                  <  2:564220/150‑1 (MQ=255)
                                                                                                                                    ccTCTTGTAACAAAAACCCCCCTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGcccc                <  2:286661/151‑1 (MQ=255)
                                                                                                                                      tctTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCaa              >  2:1555534/1‑151 (MQ=255)
                                                                                                                                             aCAAAACCCCCACGTCGGCGGAAATGGTTGAGAAGGTCGTAGGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCAATGATGAATGCCCCAATCAGGtt       <  1:1412961/151‑1 (MQ=255)
                                                                                                                                              cAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGttt      >  1:180686/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTG  >  NC_000913/657527‑657823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑