Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,332,8230AG50.8% 119.3 / 192.4 132pseudogene (1580/4605 nt)yfaSpseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base A (16/49);  new base G (67/0);  total (83/49)
Fisher's exact test for biased strand distribution p-value = 1.39e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-13

Only 100 of 134 total aligned reads displayed.

GCTATTACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCG  >  NC_000913/2332675‑2332970
                                                                                                                                                     |                                                                                                                                                        
 cTATTACTGTATTGTGCGGCAGTACTTAATGGGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGgag‑a                                                                                                                                                       <  2:236744/150‑1 (MQ=255)
 cTATTACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑At                                                                                                                                                      >  2:1642499/1‑151 (MQ=255)
   aTTACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGCGACGG‑TTAACAAATAGCGGCTCGGTTTATCTGCGGCGGGGGG‑Attt                                                                                                                                                    >  1:1052590/1‑151 (MQ=255)
   aTTACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATTGGCGGCGGGGGG‑Attt                                                                                                                                                    >  2:7816/1‑151 (MQ=255)
   aTTACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑Gttt                                                                                                                                                    >  2:990216/1‑151 (MQ=255)
   aTTACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑Attt                                                                                                                                                    >  2:1153812/1‑151 (MQ=255)
   aTTACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAAACCCCGTTCAATGGGGTCCCTTTTGGTGGTGTTGACCCGTACCGCCCCGCCG‑CCCCGGCCGGGACGGTTTACCAAATACGGGCCGGGTTTTTCGGCGGGGGGGGG‑Gttt                                                                                                                                                    >  1:872833/1‑151 (MQ=255)
    ttACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGGGGGGGG‑ATTTa                                                                                                                                                   >  2:44177/1‑151 (MQ=255)
    ttACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑Gtttt                                                                                                                                                   >  1:254878/1‑150 (MQ=255)
    ttACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTa                                                                                                                                                   >  2:692395/1‑151 (MQ=255)
    ttACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑ATCACAAATAGCGGCTCGGCTTATAGGCCGCGGGGAG‑Agttt                                                                                                                                                   >  1:1488722/1‑150 (MQ=255)
       cTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACg                                                                                                                                                <  1:1620399/151‑1 (MQ=255)
        tGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGc                                                                                                                                               <  2:35351/151‑1 (MQ=255)
         gTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑GTTTTACGc                                                                                                                                               >  1:970196/1‑150 (MQ=255)
         gTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTTACGc                                                                                                                                               >  2:1243398/1‑150 (MQ=255)
         gTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGGTTTTCGGCGGCGGGGGG‑ATTTTAccc                                                                                                                                               >  2:68864/1‑148 (MQ=255)
              gtgCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑GTTTTAAGCcccaag                                                                                                                                         >  2:516048/1‑149 (MQ=255)
              gtgCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGCTTATCCGCCGCGGGGGG‑ACTTAACGCCCCAt                                                                                                                                          >  1:549396/1‑150 (MQ=255)
                 cGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCATTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGAc                                                                                                                                       <  2:281963/150‑1 (MQ=255)
                 cGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGAc                                                                                                                                       <  1:783471/150‑1 (MQ=255)
                   gCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑GATTAACGCcacaatgaca                                                                                                                                     >  1:1044132/1‑144 (MQ=255)
                      gTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑GTTTAACGCCCCATGAcccccgg                                                                                                                                 >  2:1230404/1‑146 (MQ=255)
                      gTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACCCCCCATGACCCCTgg                                                                                                                                 >  2:1357750/1‑151 (MQ=255)
                        aCTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑AATTTAAGCCCCATTAGCGCCgggg                                                                                                                               >  1:810912/1‑149 (MQ=255)
                         cTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑GATTAAAGCCACAttaaccctcggcg                                                                                                                              >  1:1427603/1‑139 (MQ=255)
                         cTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTTACGCCACATTACCGCTGGCGt                                                                                                                              >  1:746874/1‑151 (MQ=255)
                         cTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGt                                                                                                                              <  1:1154918/151‑1 (MQ=255)
                          ttAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑AATTTACGCCCCAATACCGCTggggg                                                                                                                              >  1:1721812/1‑147 (MQ=255)
                            aaTGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAAAGCCACATGACCGCTGGGGGC‑gg                                                                                                                          >  2:1523875/1‑151 (MQ=255)
                              tGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGac                                                                                                                        <  1:103927/151‑1 (MQ=255)
                               gAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTAACGCCACATGACCGCCGGCGTC‑GGgca                                                                                                                       >  2:1082703/1‑151 (MQ=255)
                               gAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑AGTTAACGCCACATGACCGCTGGccgcgggca                                                                                                                        >  1:1652938/1‑143 (MQ=255)
                                  tAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGacac                                                                                                                      <  2:485001/149‑1 (MQ=255)
                                   aaTGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGt                                                                                                                   <  1:830158/151‑1 (MQ=255)
                                      gcgcCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑GTTTTACGCCCCATGACCGCCGGCGGC‑GGACACCGtttt                                                                                                                >  1:501250/1‑150 (MQ=255)
                                      gcgcCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑GATTTACGCCACATGACCGCTGGCGTC‑GGACACCGttt                                                                                                                 >  1:854477/1‑150 (MQ=255)
                                      gcgcCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTAACGCCACATGACCGCTGGGGTC‑GGGCACCGttt                                                                                                                 >  2:1216967/1‑150 (MQ=255)
                                       cgcCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTc                                                                                                                <  1:182821/150‑1 (MQ=255)
                                        gcCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATACCGGCTCGGGCTATTGGCGGCGGGGGG‑ATTTTAAAGCACATGGCCCCTGGGGTC‑CGACACCGTTTCg                                                                                                               >  2:741458/1‑150 (MQ=255)
                                         ccAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGc                                                                                                              <  2:624691/150‑1 (MQ=255)
                                                 cgTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGCGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ACTTTAAGCCCCCTGACCCCTTGGGTC‑CGGCAccccttccccgcccttc                                                                                                      >  1:1522389/1‑135 (MQ=255)
                                                 cgTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTTCCTGCCCTcc                                                                                                      >  2:1031130/1‑150 (MQ=255)
                                                     cAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGGTATTTAA‑GCCACATGAGCGCTGGCGGCTGGACA‑CGTTTCGCCGCCCTCCAGCGa                                                                                                 >  1:860356/1‑151 (MQ=255)
                                                     cAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTAAAGCCACATGACCGCCGGCGTC‑GGACACCCGTTCGCTGCCCTcccacca                                                                                                 >  1:1012645/1‑146 (MQ=255)
                                                          aGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑GTTTAACGCCCCATGACCGCTGGCGGC‑GGACACCGTTTCGCCGCCCTCCAGCGAC‑ACgg                                                                                           >  2:923560/1‑151 (MQ=255)
                                                            gATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTTAAGCCACAAGACCGCCGGCGTC‑GGAAAACCTTTCGGTGACTTCCACCAAC‑ACgggg                                                                                         >  2:330482/1‑151 (MQ=255)
                                                              tctcTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTAACGCCACCTTACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCACCGAC‑ACGGGGaa                                                                                       >  2:344912/1‑151 (MQ=255)
                                                                 ctTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTTACGCCACATTACCGCTGGCGTC‑GGGCACCGGTTTGCTGCCTTCCAGCGGC‑ACGGGGgaaag                                                                                    >  1:1287210/1‑149 (MQ=255)
                                                                 ctTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTTACGCCACATGACCGCTGGGGTC‑GGACACCGTTTCGCTGCCTTCCCGCGAC‑ACGGGGaaaag                                                                                    >  2:800521/1‑149 (MQ=255)
                                                                 ctTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTTAAGCCACATGACCGCTGGCGTC‑GGACACCGTTTTGCTGCCTTCCCGCGAC‑ACGGGGGAACg                                                                                    >  2:1039958/1‑151 (MQ=255)
                                                                 ctTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCCGCCTTCCAGCGAC‑ACGGGGAAACg                                                                                    >  2:896694/1‑151 (MQ=255)
                                                                 ctTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACg                                                                                    <  1:1353274/151‑1 (MQ=255)
                                                                      gtggtggtgACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑GATTAACCCCACATGACCGCTGGGGCC‑GGGCACCGGTTTGCTGCCTTCCCGGGAC‑ACGGGGGAAAGTcccg                                                                               >  2:1396764/1‑149 (MQ=255)
                                                                               aCGCGATATGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑Gc                                                                      <  1:1290316/150‑1 (MQ=255)
                                                                               aCGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑Gc                                                                      <  1:1322289/150‑1 (MQ=255)
                                                                                cgcgATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCAAATGACCGCTGGCGTC‑GGACAACGTTTCGCTGCCTTCCCGCGAC‑ACGGGGAAAAGGCCGGCCTAAA‑GCaa                                                                    >  2:565497/1‑151 (MQ=255)
                                                                                    aTACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCg                                                                <  2:299260/151‑1 (MQ=255)
                                                                                            cgccgTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGGGGGGGG‑ATTTTAACCCACATAACCGCTGGGGTC‑CGGCACCCCTTTGCTGCCCTCCCCCGAC‑AAGGGGGAAAGGCCCGCGTAAC‑GCGAAATGTTA‑cccg                                                        >  2:1277362/1‑148 (MQ=255)
                                                                                            cgccgTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGAc                                                       <  2:1214826/151‑1 (MQ=255)
                                                                                             gccgTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTAACCCCCCATGACCGCCTGGGTC‑GGGCACCGTTTTGGCGCCTTTCCGCGACAACGGGGAAA‑GTCCGGCATAACGGCAAACCGTTA‑cgac                                                        >  1:22150/1‑147 (MQ=255)
                                                                                              ccgTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGAcc                                                      <  1:931273/150‑1 (MQ=255)
                                                                                              ccgTCACTGGCGGAGACGG‑TTAACAAATAGCGGCCCGGTTTATCGGCGGCCGGGAG‑ATTTTACGCCACATTACCGCTGGCGTC‑GGCCACCCGTTTGCTGCCCTCCAGCGAC‑ACGGGGGAACGGCCGGCATAAC‑CGAAATCGTTA‑cccccca                                                     >  2:1138621/1‑146 (MQ=255)
                                                                                                  cACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTAAAGCCCCATGACCGCTGGGGGC‑GGGAACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAAACGTTA‑CCGACCATTGa                                                 >  1:1116024/1‑151 (MQ=255)
                                                                                                    cTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATa                                               <  2:518978/151‑1 (MQ=255)
                                                                                                    cTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATa                                               <  1:408894/151‑1 (MQ=255)
                                                                                                    cTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATa                                               <  1:919711/151‑1 (MQ=255)
                                                                                                    cTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATa                                               <  1:717807/151‑1 (MQ=255)
                                                                                                    cTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATa                                               <  2:1472511/151‑1 (MQ=255)
                                                                                                    cTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATa                                               <  1:1229810/151‑1 (MQ=255)
                                                                                                     tGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACAAGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATag                                              <  1:653183/151‑2 (MQ=255)
                                                                                                          gagaCGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGc                                          <  2:797213/150‑1 (MQ=255)
                                                                                                           agaCGG‑TTAACAAAGAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCCCATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAAttctg                                         <  1:261733/150‑4 (MQ=255)
                                                                                                             aCGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGCTg                                        <  2:1246527/149‑1 (MQ=255)
                                                                                                              cGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGGAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTTATAATTGCTGAg                                      >  2:886556/1‑150 (MQ=255)
                                                                                                              cGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTAACGCCACATGACCGCTGGCGGC‑GGACACCGTTTTGCTGCCTTCCAGCGAC‑ACGGGGAAAAGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCCTTGATAATTTCTGAg                                      >  2:975579/1‑150 (MQ=255)
                                                                                                                g‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTAACGCCACATGACCGCTTGCGGC‑GGACACCGCTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGGCCCGCATAAC‑GCAAAGCGGTACCCGACC‑TTGATTATTTCTTAgcg                                    >  1:723203/1‑150 (MQ=255)
                                                                                                                g‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTTCTGAgcg                                    >  2:553907/1‑150 (MQ=255)
                                                                                                                g‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTTATAATTGCTGAgcg                                    >  2:152124/1‑150 (MQ=255)
                                                                                                                g‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGCTGAgcg                                    <  1:1661982/150‑1 (MQ=255)
                                                                                                                g‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGGTTCGCTGCCTTCCAGCGAC‑ACGGGGGAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGCTGAgcg                                    >  1:700267/1‑150 (MQ=255)
                                                                                                                g‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑AATTAACGGCACATTACCGCCCGGGGG‑GGAAACCCGTTTGGTGGCTTCCAGAGAC‑ACGGGGAAAAGGACGGGATCAC‑CCAAAACGTTT‑ACGACCATTGATAAATGCCGAggcg                                   >  1:489337/1‑148 (MQ=255)
                                                                                                                     aCAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTAACGCCCCATGACCGCTGGCGTC‑GGACACCGGTTTGCTGCCTTCCCGCGAC‑ACGGGGGAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTTATAATTTCTGAGCGCGCaa                               >  1:1529720/1‑151 (MQ=255)
                                                                                                                     aCAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTAACGCCACATGACCGCTGGGGTC‑GGACACCGTTTCGCTGCCTTCCCGCGCA‑CCGGGGGAACGGCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGCTGGGCGGGCaa                               >  2:1195501/1‑151 (MQ=255)
                                                                                                                     aCAAATAGCGGCTCGGTTTATCGGCGGCGGGGGG‑ATTTAACGCCACAAGACCGCTGGCGTC‑GGGCACCGTTTCGCGGCCTTCCAGCGAC‑ACGGGGGAACGTCCGGCCTAAC‑CCAAAACGTTA‑CCGACCATTGATAATTGCTGAGCGCGCaa                               >  1:1717176/1‑151 (MQ=255)
                                                                                                                       aaaTAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCCGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGCC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTTCTGAGCGCGCaaa                              >  2:1479937/1‑150 (MQ=255)
                                                                                                                        aaTAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGCTGAGCGCGCAAACt                            <  2:1634967/151‑1 (MQ=255)
                                                                                                                          tAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTTACGCCCCATGACCGCTGGCGTC‑CGACACCGTTTCGCTGCCTTTCAGCGAC‑ACGGGGAAACGGCCGGCATAAC‑GCAAATCGTTT‑CCGACCATTGATAATTGCTGAGCGCGCAAACTTa                          >  1:994178/1‑151 (MQ=255)
                                                                                                                               gCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTg                      <  1:395799/150‑1 (MQ=255)
                                                                                                                               gCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTg                      <  2:1432909/150‑1 (MQ=255)
                                                                                                                                cTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGc                     <  1:1433309/150‑1 (MQ=255)
                                                                                                                                  cGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCAcg                  <  1:663431/151‑1 (MQ=255)
                                                                                                                                   ggTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGGTTCGCTGCCTTCCCGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCCTTGATAATTGCTGAGCGCGCAAACTTAACTGCAcg                  >  1:439756/1‑150 (MQ=255)
                                                                                                                                    gTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCAcgcg                >  1:944410/1‑151 (MQ=255)
                                                                                                                                    gTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGGTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCAcgcg                >  1:1236110/1‑151 (MQ=255)
                                                                                                                                            gcggcggGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCAttt         <  2:1011212/150‑1 (MQ=255)
                                                                                                                                                  gggAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTAcc   <  2:437357/150‑1 (MQ=255)
                                                                                                                                                  gggAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTAcc   <  1:1039411/150‑1 (MQ=255)
                                                                                                                                                  gggAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTAcc   <  2:39540/150‑1 (MQ=255)
                                                                                                                                                  gggAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTAcc   <  1:486304/150‑1 (MQ=255)
                                                                                                                                                  gggAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCg  >  1:870615/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                        
GCTATTACTGTATTGTGCGGCAGTACTTAATGAGTAATGCGCCAGACCGCGTTCAATGAGGATCTCTTTGGTGGTGGTGACGCGATACGCCGCGCCGTCACTGGCGGAGACGG‑TTAACAAATAGCGGCTCGGTTTATCGGCGGCGGGGAG‑ATTTAACGCCACATGACCGCTGGCGTC‑GGACACCGTTTCGCTGCCTTCCAGCGAC‑ACGGGGAAACGTCCGGCATAAC‑GCAAATCGTTA‑CCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCG  >  NC_000913/2332675‑2332970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑