Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,443,2770AC45.1% 126.0 / 50.5 91V274G (GTA→GGA) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (47/3);  new base C (0/41);  total (47/44)
Fisher's exact test for biased strand distribution p-value = 9.97e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATA  >  NC_000913/3443128‑3443424
                                                                                                                                                         |                                                                                                                                                   
cAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTa                                                                                                                                                     >  1:157470/1‑150 (MQ=255)
   aCGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAccc                                                                                                                                                  <  2:970741/150‑1 (MQ=255)
    cGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGCAGCGAAGATTGCCGGGATTAcccc                                                                                                                                                 >  1:622861/1‑150 (MQ=255)
       aTAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGcc                                                                                                                                             <  1:249438/151‑1 (MQ=255)
          gagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCata                                                                                                                                          >  2:344317/1‑151 (MQ=255)
          gagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCata                                                                                                                                          >  2:1037765/1‑151 (MQ=255)
          gagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCata                                                                                                                                          >  1:526597/1‑151 (MQ=255)
          gagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCata                                                                                                                                          >  2:733253/1‑151 (MQ=255)
          gagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCata                                                                                                                                          >  2:868902/1‑151 (MQ=255)
            gTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATAtt                                                                                                                                        >  2:925395/1‑151 (MQ=255)
                cacaTAAAGGGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCa                                                                                                                                      >  1:669480/1‑149 (MQ=255)
                cacaTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCa                                                                                                                                      >  2:145910/1‑149 (MQ=255)
                 acaTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACtt                                                                                                                                   >  2:631791/1‑151 (MQ=255)
                  caTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACttt                                                                                                                                  >  2:1206837/1‑151 (MQ=255)
                  aaaaaggCGGTCCCCCGGGCTGAAAACACAGAAAATTGTTGTCCAGCCATTCCCAACCATCCCCCCCCCCGAACCATGCCGGGGTGGTCCCCGGAAAAAAAATAATTCGGGAAGAGAAGTTCGCGGTAATCCCCCCCGCCATATTCACttt                                                                                                                                  <  1:641501/145‑1 (MQ=255)
                          ggTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTa                                                                                                                          >  1:1305433/1‑151 (MQ=255)
                           gTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTaa                                                                                                                         >  1:442327/1‑151 (MQ=255)
                           gTTCCCCAGGCTGAAAACACAGCAAATTTGTTGCCAGCAAGTCCCACCCAGTCCCGCCCCCGACCCATGCGGGGATGGTCGCCGGAAAAAAAATAATCCTGGAAGGGAATTTGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTa                                                                                                                          <  1:289804/150‑1 (MQ=255)
                                ccagccagCAAATCCAGCGAATTTGTTGTCAGCCATTCCCAACCAACCCCCCCCCAAACCAATGCCGCGATGTTCGCCGGAAACAAAATAATACTGGAAGCAAATTTGGCCGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtgtg                                                                                                                    <  1:1281094/151‑1 (MQ=255)
                                     cgaaaaaaACAGCAAAATTGTTGTCGGCCATTCCCACCCAGTCCCGCCCCCAAACCAGGACGCGGTGGTCGCGGGGAACAAAATAATAGGGGAGGGGAAGTTGGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc                                                                                                               <  1:405138/147‑1 (MQ=255)
                                     cgaaaaaTACAGCGAATTTGTTGTCAGCCATTCCCAACCATCCCCCCCCCCGAACCAGGACGCGGGGGTCGCCGGAAAAAAAATAAGACGGGAAGCGAAAATGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc                                                                                                               <  2:346504/147‑1 (MQ=255)
                                     cTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGct                                                                                                                >  2:396120/1‑150 (MQ=255)
                                     cTGAAAATACAGAAAAATTGTTGTCAGCCAGTCCCACCCAGTCCCCCCCCCGAACCATGACGCGAGGGTCGCGGGAAACAAAATAATACGGGAAGGAAAGTTTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc                                                                                                               <  2:876885/151‑1 (MQ=255)
                                       gCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGct                                                                                                                >  1:1247191/1‑148 (MQ=255)
                                          aaaaCAGCAAATTTGTTGTCAGCCATTCCCACCCAGTCCCCCCCCCAACCCAGGCCGGGAGGGCCGCGGGAAAAAAAAAAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgc                                                                                                          <  2:526817/148‑1 (MQ=255)
                                             acggcaaatttgttgCCAGCCATTTCCACCCAGTCCCGCCCCCGAACAATGGCGCGATGGCCGCCGGAAAAAAAATAATCCGGGAAGCAAAGTTGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagc                                                                                                       <  2:391116/142‑1 (MQ=255)
                                                 gaaatttgttgTCAGCCAGTCCCAACAAGCCCCCCCCCCGAACCATGACGGGAGGGCCGCCGGGAAAAAAAAAATACGGGAAGCGAATATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                     <  2:1132314/144‑1 (MQ=255)
                                                 caaatttgttgCCAGCCAGCACCAACCAGTCCCGCCCCCAACCAATGACGCGATGGTCCCCGGAAACAAAATAATGTTGGAAGCGAATTTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                     <  1:147878/144‑1 (MQ=255)
                                                 aaaatttgttgCCACCCAGTCCAACCCAGCCCCCCCCCCAACCCAGGACGCGATGGCCGCGGGAAACAAAAAAATCGGGAAAGGAAATATGGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                     <  1:1693306/144‑1 (MQ=255)
                                                  gaaattttttgccggCCAGTCCCACCCAGTCCCCCCCCCAAACCATGACGCGATGGCCGCCGGGAAAAAAAAAATCCGGGAAGCGAAGATGGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                     <  2:175539/135‑1 (MQ=255)
                                                   ataTTGTTGGCGGCCAGTTCCACCAAGTCCCGCCCCGGACCCGTGGCGCGGTGGTCCCCGGAAACAAAATAATAGTGGAAGCGAAGTTTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                                 <  1:497010/149‑1 (MQ=255)
                                                   aatttgttgTCAGCCAGTTCCACCCAGCCCCCCCCCCGAACCATGCCGCGTTGGTCGCGGGAAAAAAAAAAATACTGAAAGGAAAGATTGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                                 <  2:115992/148‑1 (MQ=255)
                                                   aaaTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGa                                                                                                  >  2:158615/1‑150 (MQ=255)
                                                   aaaTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGa                                                                                                  >  1:797242/1‑150 (MQ=255)
                                                   aaaTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGa                                                                                                  >  2:1334348/1‑150 (MQ=255)
                                                    attttttgtCAGCCTGTCCCACCCAGTCCCCCCCCCGAACCATTACGCGATGTTCGCCGGGAACAAAATAATCCTGGAAGCAAAGATTGCCGGGTTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGaca                                                                                                <  1:946868/146‑1 (MQ=255)
                                                    atttgttgTCAGCCAGTCCCACCCACCCCCCCCCCCAACCAAGGACGCGATGGTCGCCGAGAACAAAATAATACGGGAGGGAAATATGGCGGGGTTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGaca                                                                                                <  1:370147/149‑1 (MQ=255)
                                                    aaTTTTTTTCACCCAGTTCAACCCAGCCCCCCCCCGAACCCAGGACGGGATGGCCGCGGGAAACAAAATAATACGGGAAGGGAAGATTGCAGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGaca                                                                                                <  2:10596/151‑1 (MQ=255)
                                                         ttgtCAGCAAGTCCAAACCAGTCCCGCCCCCAAACCAGGAGGGGATGGTCGCCGGAAAAAAAAAAATACGGGAAGCAAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                            <  2:1190430/150‑1 (MQ=255)
                                                                        cAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGtt                                                                             >  2:1153583/1‑151 (MQ=255)
                                                                         aaCCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                            >  1:808438/1‑151 (MQ=255)
                                                                         aaCCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                            >  2:1064204/1‑151 (MQ=255)
                                                                              gTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt                                                                       >  2:1688285/1‑151 (MQ=255)
                                                                               tcccGCCCCCAACCCGGGACGCTAGGGTCGCCGGAAACAAAATAATTCTGGAAGCGAATATTGCCGGGATCCCCCCCGCTATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt                                                                       <  1:1194235/148‑1 (MQ=255)
                                                                                   ccccccGACCCAGGGCGCGTTGTTCCCCGGAAACAAAAAAATTCTGGAAGCAAAGATTGCGGTGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                                                                  <  1:400085/150‑1 (MQ=255)
                                                                                    cccccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTa                                                                 >  2:492227/1‑151 (MQ=255)
                                                                                    cccccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTa                                                                 >  1:1399916/1‑151 (MQ=255)
                                                                                     ccccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTa                                                                 >  1:1684442/1‑150 (MQ=255)
                                                                                        caaaCCATGACGGGGTGGCGGCGGGAAAAAAAATAATACTGGAAGCAAAGATTGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                             <  2:808893/149‑1 (MQ=255)
                                                                                        caaaCCATGACGCGATGGGCCCGGGGAAAAAAATAACCGGGGAAGAAAATGTTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                             <  2:1719433/149‑1 (MQ=255)
                                                                                        caaaCCAGGACGCGAGGGTCGCGGAGAAAAAAATAATGCTGGAAGAAAAGTTGGCCGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                             <  1:563798/149‑1 (MQ=255)
                                                                                          caacccatgcgggggtGGCCGCGGGAAAAAAAATAATACTGGAAGAAAATTTTGCGGGTATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                             <  1:241626/136‑1 (MQ=255)
                                                                                          caaacgatggggtgATGGGCCCGGGGAACAAAAAAAGCCGGGAAGGGAAGATCGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                             <  2:481314/138‑1 (MQ=255)
                                                                                          aaCCAGGACGGGATGGTCGCCGAAAAAAAAATAAGAGGGGAAGCGAAGAGTGCGGGGTTTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACaa                                                           <  2:1222528/151‑1 (MQ=255)
                                                                                           aCCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAAt                                                          >  1:382706/1‑151 (MQ=255)
                                                                                              cATGCCGGGAGGGTCGCCGGAAAAAAAATAATACGGGAAGGGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgc                                                        <  2:1158612/151‑1 (MQ=255)
                                                                                                    cgcgATGGTCGCGGGGAAAAAAATAATACGGGAAGCGAAGTTGTCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGt                                                   <  2:1684794/151‑1 (MQ=255)
                                                                                                               cgggaaaaaaaataatCTTGGAGGAGAAGTTTGCGGGGTTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCt                                         <  1:112198/140‑1 (MQ=255)
                                                                                                                   gAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaaca                                    >  2:1662579/1‑151 (MQ=255)
                                                                                                                   gAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaaca                                    >  2:1215207/1‑151 (MQ=255)
                                                                                                                   gAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaaca                                    >  1:652327/1‑151 (MQ=255)
                                                                                                                   gAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaaca                                    >  1:27455/1‑151 (MQ=255)
                                                                                                                   aaaaagaaaaaagggggaagggaagGTTGCCGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaaca                                    <  2:83180/136‑1 (MQ=255)
                                                                                                                      cAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACGCGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                 >  2:850233/1‑151 (MQ=255)
                                                                                                                       aGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                >  2:275576/1‑151 (MQ=255)
                                                                                                                        gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAGATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                >  1:1457831/1‑150 (MQ=255)
                                                                                                                        gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTTAACAAATa                                >  2:1065428/1‑150 (MQ=255)
                                                                                                                        gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                >  2:1722420/1‑150 (MQ=255)
                                                                                                                        gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                >  2:523244/1‑150 (MQ=255)
                                                                                                                        gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                >  1:1038937/1‑150 (MQ=255)
                                                                                                                        gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                >  2:151743/1‑150 (MQ=255)
                                                                                                                        gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                >  2:147702/1‑150 (MQ=255)
                                                                                                                        gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                >  1:1115284/1‑150 (MQ=255)
                                                                                                                        gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                >  1:54958/1‑150 (MQ=255)
                                                                                                                        gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                >  2:1145485/1‑150 (MQ=255)
                                                                                                                        gAATAATACTGGAAGCGAAGATCGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAAAGCGGCGTTGACCACGCTCAACAAATa                                >  1:369943/1‑150 (MQ=255)
                                                                                                                        aaataatGCTGGAAGAGAAGTTGGGCGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                               <  1:1119975/150‑1 (MQ=255)
                                                                                                                        aaataatATTGGAAGCGAAGTTTGCGGTGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                               <  1:258176/150‑1 (MQ=255)
                                                                                                                        aaataatACGGGAAGGAAATGTTGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                               <  1:1282133/150‑1 (MQ=255)
                                                                                                                        aaaaaatcCTGGAGGCGAAGTTCGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                               <  2:1587763/147‑1 (MQ=255)
                                                                                                                          ataataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaa                             >  1:1577967/1‑151 (MQ=255)
                                                                                                                                tgggAAGCGAAGAGTGACGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGa                       <  1:1516024/149‑1 (MQ=255)
                                                                                                                                   gacgctaattttGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAAc                     <  1:492040/140‑1 (MQ=255)
                                                                                                                                        caaagttGGCGGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTc                  <  2:1351201/144‑1 (MQ=255)
                                                                                                                                         aaGATTGGCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTg              <  1:750190/151‑1 (MQ=255)
                                                                                                                                            ttgggggagATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaa           <  1:1623366/143‑1 (MQ=255)
                                                                                                                                                ccGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaata        <  1:1273296/150‑1 (MQ=255)
                                                                                                                                                ccGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaata        <  1:331049/150‑1 (MQ=255)
                                                                                                                                                 cGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCACTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaata        >  2:1057737/1‑149 (MQ=255)
                                                                                                                                                  gggTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact      <  2:1272932/150‑1 (MQ=255)
                                                                                                                                                   ggATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaa    >  1:1605942/1‑151 (MQ=255)
                                                                                                                                                    gATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaat   >  2:960234/1‑151 (MQ=255)
                                                                                                                                                         aCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaata  >  2:1289543/1‑148 (MQ=255)
                                                                                                                                                         aCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaata  >  1:579341/1‑148 (MQ=255)
                                                                                                                                                         |                                                                                                                                                   
CAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATA  >  NC_000913/3443128‑3443424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: