Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,168,350 Δ1 bp coding (483/633 nt) ycfQ ← repressor for bhsA(ycfR)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,168,3480G.100.0% 167.9 / NA 40coding (485/633 nt)ycfQrepressor for bhsA(ycfR)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (20/20);  total (20/20)

TTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTT  >  NC_000913/1168212‑1168469
                                                                                                                                        |                                                                                                                         
ttCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCgga                                                                                                                         >  2:706824/1‑138 (MQ=255)
  cGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCggatt                                                                                                                       >  1:119362/1‑136 (MQ=255)
  cGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCggatt                                                                                                                       >  2:106259/1‑136 (MQ=255)
     gCCATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTcc                                                                                                                   >  1:366400/1‑139 (MQ=255)
        aTAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCcg                                                                                                                <  2:366400/139‑1 (MQ=255)
                                       cAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCa                                                                                 <  1:629691/139‑1 (MQ=255)
                                            tttCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                            <  1:124951/139‑1 (MQ=255)
                                                      tGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCa                                                                  <  2:619033/139‑1 (MQ=255)
                                                             tCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATg                                                           >  2:725576/1‑139 (MQ=255)
                                                                                     tGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGat                                   <  1:385279/139‑1 (MQ=255)
                                                                                     tGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGat                                   <  1:493759/139‑1 (MQ=255)
                                                                                     tGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGat                                   >  1:615546/1‑139 (MQ=255)
                                                                                      gAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGata                                  <  1:725576/139‑1 (MQ=255)
                                                                                            taCAATTATGGAATTTTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAGGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCt                            >  1:534471/1‑139 (MQ=255)
                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTTTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGc                                                         >  2:137522/1‑110 (MQ=255)
                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTTTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGc                                                         <  1:137522/110‑1 (MQ=255)
                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATaaa                                                                                           >  2:639230/1‑76 (MQ=255)
                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATaaa                                                                                           <  1:639230/76‑1 (MQ=255)
                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAGAACTGCTGCAAAGTg                                                                            >  1:514966/1‑91 (MQ=255)
                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCa                                                                                 >  1:336395/1‑86 (MQ=255)
                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCa                                                                                 <  2:336395/86‑1 (MQ=255)
                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                            <  2:514966/91‑1 (MQ=255)
                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCt                            >  1:802197/1‑139 (MQ=255)
                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCt                            >  2:279942/1‑139 (MQ=255)
                                                                                                 ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                            >  2:321322/1‑86 (MQ=255)
                                                                                                 ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                            <  1:321322/86‑1 (MQ=255)
                                                                                                 ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGAttt                                                  >  2:146895/1‑112 (MQ=255)
                                                                                                 ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGAttt                                                  <  1:146895/112‑1 (MQ=255)
                                                                                                 ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATc                       >  2:319547/1‑139 (MQ=255)
                                                                                                 ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATc                       >  1:633433/1‑139 (MQ=255)
                                                                                                      gAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTgggg                  >  2:382480/1‑139 (MQ=255)
                                                                                                       aaTTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGga                 <  1:706824/139‑1 (MQ=255)
                                                                                                         ttCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGgaga               >  1:20563/1‑139 (MQ=255)
                                                                                                           cTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAgg             >  2:602606/1‑139 (MQ=255)
                                                                                                            tGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAGAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAggg            <  2:569095/139‑1 (MQ=255)
                                                                                                             gCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGggt           <  2:346620/139‑1 (MQ=255)
                                                                                                                 gATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGgtggtg       <  2:633433/139‑1 (MQ=255)
                                                                                                                 gATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGgtggtg       <  1:382480/139‑1 (MQ=255)
                                                                                                                      gtcacgtcacAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAgtt  >  1:299997/1‑139 (MQ=255)
                                                                                                                       tcacgtcacAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAAc                                                                                        <  1:303842/52‑1 (MQ=255)
                                                                                                                       tcacgtcacAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAAc                                                                                        >  2:303842/1‑52 (MQ=255)
                                                                                                                                aGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                            >  2:176127/1‑55 (MQ=255)
                                                                                                                                aGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                            <  1:176127/55‑1 (MQ=255)
                                                                                                                                        |                                                                                                                         
TTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTT  >  NC_000913/1168212‑1168469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: