Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,370,044 (T)9→8 intergenic (+41/‑172) pspE → / → ycjM thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,370,0360T.100.0% 158.0 / NA 38intergenic (+33/‑180)pspE/ycjMthiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (17/21);  total (17/21)

GTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTG  >  NC_000913/1369904‑1370169
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gTCAGGGCAAGCAAAAGAGGTCTTTAGGGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCTTGAAAGACATGGCAATGCCGAAGGTCAAATTTTATAAGAATTCATCGTGTTCGGTGCTTATGCGCCGttttttt                                                                                                                                 <  1:265185/139‑1 (MQ=255)
gTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGttttttt                                                                                                                                 >  1:2414/1‑139 (MQ=255)
      gcaagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTc                                                                                                                          >  1:427480/1‑139 (MQ=255)
       caagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTca                                                                                                                         <  1:168693/139‑1 (MQ=255)
       caagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTca                                                                                                                         <  1:50890/139‑1 (MQ=255)
        aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTcac                                                                                                                        <  1:316962/139‑1 (MQ=255)
        aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTcac                                                                                                                        <  2:708881/139‑1 (MQ=255)
        aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTcac                                                                                                                        <  2:256861/139‑1 (MQ=255)
        aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTcac                                                                                                                        <  2:440458/139‑1 (MQ=255)
          gcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCacac                                                                                                                      >  2:128020/1‑139 (MQ=255)
                   aTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTAc                                                                                                             >  1:39799/1‑139 (MQ=255)
                     ccTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAATGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACAt                                                                                                           <  2:367798/139‑1 (MQ=255)
                              gaTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGa                                                                                                  <  1:585191/139‑1 (MQ=255)
                               aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGat                                                                                                 >  1:8927/1‑139 (MQ=255)
                                        aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                        <  1:416666/139‑1 (MQ=255)
                                         cccACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTcac                                                                                                                        <  1:560489/106‑1 (MQ=255)
                                         cccACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTcac                                                                                                                        >  2:560489/1‑106 (MQ=255)
                                           cACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAg                                                                                     <  2:463170/139‑1 (MQ=255)
                                             cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   >  1:564049/1‑139 (MQ=255)
                                             cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   >  2:272426/1‑139 (MQ=255)
                                              gTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTc                                                                                                                          <  1:54700/99‑1 (MQ=255)
                                              gTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTc                                                                                                                          >  2:54700/1‑99 (MQ=255)
                                              gTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTc                                                                                  >  2:213400/1‑139 (MQ=255)
                                                        ccGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTc                                                                                                                          >  2:74397/1‑89 (MQ=255)
                                                        ccGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTc                                                                                                                          <  1:74397/89‑1 (MQ=255)
                                                        ccGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACa                                                                                                            <  2:292755/103‑1 (MQ=255)
                                                        ccGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACa                                                                                                            >  1:292755/1‑103 (MQ=255)
                                                              gCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTc                                                                  >  1:701310/1‑139 (MQ=255)
                                                                   aaaGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATa                                                                           <  2:333878/125‑1 (MQ=255)
                                                                   aaaGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATa                                                                           >  1:333878/1‑125 (MQ=255)
                                                                          tCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaaa                                                      >  1:645750/1‑139 (MQ=255)
                                                                                       ggTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGt                                         <  2:564049/139‑1 (MQ=255)
                                                                                          cAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGAc                                      <  2:39799/139‑1 (MQ=255)
                                                                                                  aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAAccc                              >  1:250003/1‑139 (MQ=255)
                                                                                                      gAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtgt                          <  2:2414/139‑1 (MQ=255)
                                                                                                         ttCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                        >  1:694906/1‑74 (MQ=255)
                                                                                                         ttCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                        <  2:694906/74‑1 (MQ=255)
                                                                                                          tCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTc                      >  2:123501/1‑139 (MQ=255)
                                                                                                                           tATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtttt     <  1:213400/139‑1 (MQ=255)
                                                                                                                              gcgcCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTg  <  1:128020/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                     
GTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTG  >  NC_000913/1369904‑1370169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: