Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,240,754	(C)5→6	coding (915/1041 nt)	galS ←	galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,240,749	1	.	C	100.0%	153.9 / NA	43	A307G (GCA→GGA)	galS	galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor, mgl operon transcriptional repressor, autorepressor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (19/24);  total (19/24)

GTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑CCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGA  >  NC_000913/2240627‑2240880                                                                                                                            |                                                                                                                                    gTTACATCGCCTGATTTGTTTAGTTAGTGATCGCCGCCGCATACTGGCGTGTTGCGAGAGAATGGCTAAGCACTAACGTCGGCGGAAAACAGTGGCTGGGACGAGGAACAATAGAGCATTCTGTACCCCTGCAAGGGCa                                                                                                                      >  1:34677/1‑139 (MQ=255) gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCa                                                                                                                      >  2:83408/1‑139 (MQ=255) gTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCa                                                                                                                      >  1:268429/1‑139 (MQ=255)    aCATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGtt                                                                                                                   >  1:179594/1‑139 (MQ=255)    aCATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGtt                                                                                                                   <  2:279889/139‑1 (MQ=255)          ccTGATTTGTTGAGTTAGAGATCGCCGCCGCATTCTGGCCTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTgg                                                                                                             >  2:773666/1‑139 (MQ=255)              aTTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTaa                                                                                                         >  2:411554/1‑139 (MQ=255)                ttgttgAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAAtt                                                                                                       >  2:359457/1‑139 (MQ=255)                        ttAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCAtt                                                                                               <  2:420268/139‑1 (MQ=255)                        ttAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCAtt                                                                                               <  2:210441/139‑1 (MQ=255)                          aGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                             <  2:727402/139‑1 (MQ=255)                          aGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGa                                                                                             <  2:463070/139‑1 (MQ=255)                               tCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCaa                                                                                        <  1:146813/139‑1 (MQ=255)                                cgccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACCCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCACGGCCAGTTCGGTGGATAATTTCGCCATTGAAGCAAt                                                                                       >  1:494536/1‑139 (MQ=255)                                 gccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATg                                                                                      >  2:33237/1‑139 (MQ=255)                                  ccgccgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATgg                                                                                     >  2:174064/1‑139 (MQ=255)                                      cgcATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATa                                                                                 >  1:170493/1‑139 (MQ=255)                                           tggcgtgtgtTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            <  1:62197/133‑1 (MQ=255)                                             tGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACg                                                                          <  2:422180/139‑1 (MQ=255)                                              ggCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACgg                                                                         <  1:394273/139‑1 (MQ=255)                                               gCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            >  2:494440/1‑135 (MQ=255)                                               gCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            <  1:494440/135‑1 (MQ=255)                                                   gtTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGtt                                                                    <  1:773666/139‑1 (MQ=255)                                                                       cACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACg                                                >  2:631601/1‑139 (MQ=255)                                                                          tAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGc                                             <  2:529882/139‑1 (MQ=255)                                                                          tAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGc                                             >  1:529882/1‑139 (MQ=255)                                                                             cGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGAt                                          <  1:728220/139‑1 (MQ=255)                                                                                 ggCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGa                                      <  2:179594/139‑1 (MQ=255)                                                                                          cAGTGGCTGGCACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCg                             <  2:505927/139‑1 (MQ=255)                                                                                                ctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCg                                                             <  1:363323/101‑1 (MQ=255)                                                                                                ctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCg                                                             >  2:363323/1‑101 (MQ=255)                                                                                                ctggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCg                       <  2:237655/139‑1 (MQ=255)                                                                                                 tggcACGATGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGCATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCga                      <  2:475790/139‑1 (MQ=255)                                                                                                 tggcACGAGGATCAGTATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCga                      <  1:692878/139‑1 (MQ=255)                                                                                                 tggcACGAGGATCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCga                      <  1:631601/139‑1 (MQ=255)                                                                                                            tCAATATTGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTgagaga           <  1:359457/139‑1 (MQ=255)                                                                                                                   tGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGAt                                          >  1:545404/1‑101 (MQ=255)                                                                                                                   tGCCTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGAt                                          <  2:545404/101‑1 (MQ=255)                                                                                                                     gctgctgcCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGa  >  1:499718/2‑139 (MQ=255)                                                                                                                     ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCACTGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGa  >  1:250637/1‑139 (MQ=255)                                                                                                                     ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            >  1:625466/1‑65 (MQ=255)                                                                                                                     ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCa                                                                            <  2:625466/65‑1 (MQ=255)                                                                                                                     ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTaa      <  1:390284/135‑1 (MQ=255)                                                                                                                     ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTaa      >  2:390284/1‑135 (MQ=255)                                                                                                                     ccTGCTGCCCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGa  >  1:349336/1‑139 (MQ=255)                                                                                                                            |                                                                                                                                    GTTACATCGCCTGATTTGTTGAGTTAGTGATCGCCGCCGCATTCTGGCGTGTTGCGACAGAATGGCGACGCACTAACGTCGGCATAAAACAGTGGCTGGCACGAGGATCAATATTGCCTGCTG‑CCCCCTGCAAGGCCAGTTCGGTGGCTAATTTCGCCATTGAAGCAATGGGATAACGCACGGTCGTTAATTGCGGGTCGGTGTAACGGGCGATGGGAATATCATCGAAACCGATGATTGAGAGATGTAACGGA  >  NC_000913/2240627‑2240880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑