Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,767,445 (C)7→8 intergenic (+90/‑265) rnlB → / → yfjP CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,767,4381.C81.1% 102.9 / 17.0 37intergenic (+83/‑272)rnlB/yfjPCP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein
Reads supporting (aligned to +/- strand):  ref base . (6/1);  new base C (11/19);  total (17/20)
Fisher's exact test for biased strand distribution p-value = 3.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAA  >  NC_000913/2767301‑2767568
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aGCCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAc                                                                                                                                     >  2:30810/1‑139 (MQ=255)
  ccTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAGTTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAcca                                                                                                                                  >  2:424865/1‑138 (MQ=255)
  ccTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAccc                                                                                                                                 <  2:695383/139‑1 (MQ=255)
  ccTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGACCAATATGATCAAATAGCATTAAcat                                                                                                                                   >  1:36484/1‑137 (MQ=255)
   ctctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAcccc                                                                                                                                <  1:451351/139‑1 (MQ=255)
    tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAccccc                                                                                                                               <  1:722855/139‑1 (MQ=255)
            gTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAGCTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                        >  1:566640/1‑139 (MQ=255)
                cccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGt                                                                                                                    <  2:217938/139‑1 (MQ=255)
                cccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAc                                                                                                                                     >  2:277427/1‑123 (MQ=255)
                cccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAc                                                                                                                                     <  1:277427/123‑1 (MQ=255)
                 ccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTc                                                                                                                   <  2:566640/139‑1 (MQ=255)
                 ccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTc                                                                                                                   <  2:118757/139‑1 (MQ=255)
                  cGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCt                                                                                                                 >  1:444778/1‑139 (MQ=255)
                         gTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGTATTAACCCCCCCCTcaca                                                                                                                        <  1:443764/126‑1 (MQ=255)
                         gTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                        >  2:443764/1‑126 (MQ=255)
                           aCGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACAAACCTCTCACAACGTTCTTCAttagg                                                                                                         >  2:786295/1‑135 (MQ=255)
                              ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                        <  2:205768/121‑1 (MQ=255)
                              ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGt                                                                                                                    >  1:246505/1‑125 (MQ=255)
                              ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGt                                                                                                                    <  2:246505/125‑1 (MQ=255)
                              ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAac                                                                                                      <  2:690669/139‑1 (MQ=255)
                              ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCAAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                        >  1:205768/1‑121 (MQ=255)
                               cAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAac                                                                                                      >  1:675218/1‑138 (MQ=255)
                               cAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAac                                                                                                      <  2:675218/138‑1 (MQ=255)
                                  gTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGt                                                                                                  >  2:643875/1‑139 (MQ=255)
                                   tCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGtt                                                                                                 <  1:295686/139‑1 (MQ=255)
                                    ccTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCa                                                                                                              >  1:282196/1‑125 (MQ=255)
                                    ccTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCa                                                                                                              <  2:282196/125‑1 (MQ=255)
                                                 ttttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc                                                                                   <  1:643875/139‑1 (MQ=255)
                                                                 tctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACAACTAAATTTccc                                                                   <  1:424865/139‑1 (MQ=255)
                                                                         aaCCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTa                                                           <  2:235331/139‑1 (MQ=255)
                                                                             aGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACtt                                                      <  2:444778/139‑1 (MQ=255)
                                                                              gTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACtt                                                      <  1:382365/139‑1 (MQ=255)
                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTc                                                                                                                   >  2:555970/1‑67 (MQ=255)
                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTc                                                                                                                   <  1:555970/67‑1 (MQ=255)
                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCCCAACGTCCTGGATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTc                                          >  2:117255/1‑139 (MQ=255)
                                                                                                                 gTCGAATATGATCAAATAGCATTAACCCCCCCCTCACACCGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAAGCCTatg                   <  1:556224/139‑1 (MQ=255)
                                                                                                                 gTCCAATATGATCAAATAGCATTAAACCCCCACTCACAACGTCCTGCATAGGGAACACGTATTCCCCTCTGCACCCACGACGAAATTTTCCCCCCTATAAACTTCTTAATAATGTCAACAATTATCTGGCAATCCTttg                   >  1:473268/1‑139 (MQ=255)
                                                                                                                 gTCCAATATGATCAAATAGCATTAACCACCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCCCCCACGACTAAATTTCTCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatg                   >  1:51426/1‑139 (MQ=255)
                                                                                                                  tCCAATATGATCAAATAGCATTAAACCCCCCCTCACAACGTCTTGCATAGGGAACACGTTTTCCCCTGTGCCCCCCCGACTAAATTTCCCCCCCTTTACACTTCTTAATAATGTTACCAATTTTCTGGAAATCCTatga                  >  2:210565/1‑139 (MQ=255)
                                                                                                                  tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatga                  >  2:674555/1‑139 (MQ=255)
                                                                                                                  tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACATCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTACCCCACTTTAAAGTTTTCCATAATGTCACCAATTTTATGAAATTCCTacga                  >  1:428719/1‑139 (MQ=255)
                                                                                                                  tCCAATATGATCAAATAGCATTAACTCCCCCCTCTCAACCTCCTGCATAGGGAACACGTTTTTCCCTGAGCACACACGAATCAAATTCCCCCCCCTTAAACTTCTCAACAGTATTCCTAATTTTCTTGAAATCCTatga                  >  2:11790/1‑139 (MQ=255)
                                                                                                                         tGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCttt           <  2:36484/139‑1 (MQ=255)
                                                                                                                          gATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc          <  2:51426/139‑1 (MQ=255)
                                                                                                                            tCAAATAGCATTAACCCCCCCCTCACAACGTTCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCAACAATTTTCTGGAAATCCTATGATGCCTTTccc        >  1:442837/1‑139 (MQ=255)
                                                                                                                            tCAAATAGCATTAACCACCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGACACCGATTTTCTGGAACTCCTATGATGCCTTTacc        >  2:715565/1‑139 (MQ=255)
                                                                                                                                  aGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTaaa  <  1:786295/139‑1 (MQ=255)
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AGCCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAA  >  NC_000913/2767301‑2767568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: