Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,767,445 | (C)7→8 | intergenic (+90/‑265) | rnlB → / → yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,767,438 | 1 | . | C | 81.1% | 102.9 / 17.0 | 37 | intergenic (+83/‑272) | rnlB/yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
Reads supporting (aligned to +/- strand): ref base . (6/1); new base C (11/19); total (17/20) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.35e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AGCCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑C‑C‑CCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAA > NC_000913/2767301‑2767568 | aGCCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑c > 2:30810/1‑139 (MQ=255) ccTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAGTTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑c‑ca > 2:424865/1‑138 (MQ=255) ccTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑c‑c‑c < 2:695383/139‑1 (MQ=255) ccTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGACCAATATGATCAAATAGCATT‑AA‑cat > 1:36484/1‑137 (MQ=255) ctctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑c‑c‑cc < 1:451351/139‑1 (MQ=255) tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑c‑c‑ccc < 1:722855/139‑1 (MQ=255) gTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAGCTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTcaca > 1:566640/1‑139 (MQ=255) cccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGt < 2:217938/139‑1 (MQ=255) cccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑c > 2:277427/1‑123 (MQ=255) cccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑c < 1:277427/123‑1 (MQ=255) ccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGTc < 2:566640/139‑1 (MQ=255) ccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGTc < 2:118757/139‑1 (MQ=255) cGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑C‑C‑CCCCCTCACAACGTCCt > 1:444778/1‑139 (MQ=255) gTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGTATT‑AACC‑C‑CCCCCTcaca < 1:443764/126‑1 (MQ=255) gTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTcaca > 2:443764/1‑126 (MQ=255) aCGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑CAA‑ACCTCTCACAACGTTCTTCAttagg > 2:786295/1‑135 (MQ=255) ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTcaca < 2:205768/121‑1 (MQ=255) ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGt > 1:246505/1‑125 (MQ=255) ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGt < 2:246505/125‑1 (MQ=255) ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGTCCTGCATAGGGAac < 2:690669/139‑1 (MQ=255) ccAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCAAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTcaca > 1:205768/1‑121 (MQ=255) cAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGTCCTGCATAGGGAac > 1:675218/1‑138 (MQ=255) cAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGTCCTGCATAGGGAac < 2:675218/138‑1 (MQ=255) gTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGTCCTGCATAGGGAACACGt > 2:643875/1‑139 (MQ=255) tCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGTCCTGCATAGGGAACACGtt < 1:295686/139‑1 (MQ=255) ccTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGTCCTGCa > 1:282196/1‑125 (MQ=255) ccTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGTCCTGCa < 2:282196/125‑1 (MQ=255) ttttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc < 1:643875/139‑1 (MQ=255) tctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACAACTAAATTTccc < 1:424865/139‑1 (MQ=255) aaCCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTa < 2:235331/139‑1 (MQ=255) aGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑C‑C‑CCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACtt < 2:444778/139‑1 (MQ=255) gTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACtt < 1:382365/139‑1 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGTc > 2:555970/1‑67 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGTc < 1:555970/67‑1 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑C‑C‑CCCCCTCCCAACGTCCTGGATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTc > 2:117255/1‑139 (MQ=255) gTCGAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACACCGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAAGCCTatg < 1:556224/139‑1 (MQ=255) gTCCAATATGATCAAATAGCATTAAA‑C‑C‑CCCACTCACAACGTCCTGCATAGGGAACACGTATTCCCCTCTGCACCCACGACGAAATTTTCCCCCCTATAAACTTCTTAATAATGTCAACAATTATCTGGCAATCCTttg > 1:473268/1‑139 (MQ=255) gTCCAATATGATCAAATAGCATT‑AA‑C‑CACCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCCCCCACGACTAAATTTCTCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatg > 1:51426/1‑139 (MQ=255) tCCAATATGATCAAATAGCATTAAA‑C‑C‑CCCCCTCACAACGTCTTGCATAGGGAACACGTTTTCCCCTGTGCCCCCCCGACTAAATTTCCCCCCCTTTACACTTCTTAATAATGTTACCAATTTTCTGGAAATCCTatga > 2:210565/1‑139 (MQ=255) tCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatga > 2:674555/1‑139 (MQ=255) tCCAATATGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACATCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTACCCCACTTTAAAGTTTTCCATAATGTCACCAATTTTATGAAATTCCTacga > 1:428719/1‑139 (MQ=255) tCCAATATGATCAAATAGCATT‑AA‑CTC‑CCCCCTCTCAACCTCCTGCATAGGGAACACGTTTTTCCCTGAGCACACACGAATCAAATTCCCCCCCCTTAAACTTCTCAACAGTATTCCTAATTTTCTTGAAATCCTatga > 2:11790/1‑139 (MQ=255) tGATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCttt < 2:36484/139‑1 (MQ=255) gATCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc < 2:51426/139‑1 (MQ=255) tCAAATAGCATT‑AACC‑C‑CCCCCTCACAACGTTCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCAACAATTTTCTGGAAATCCTATGATGCCTTTccc > 1:442837/1‑139 (MQ=255) tCAAATAGCATT‑AA‑C‑CACCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGACACCGATTTTCTGGAACTCCTATGATGCCTTTacc > 2:715565/1‑139 (MQ=255) aGCATT‑AACC‑C‑CCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTaaa < 1:786295/139‑1 (MQ=255) | AGCCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑C‑C‑CCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAA > NC_000913/2767301‑2767568 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |