Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	544,754	C→T	R180H (CGT→CAT)	allC ←	allantoate amidohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	544,754	0	C	T	100.0%	96.6 / NA	31	R180H (CGT→CAT)	allC	allantoate amidohydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/8);  total (23/8)

CCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCA  >  NC_000913/544641‑544887                                                                                                                  |                                                                                                                                         ccGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAag                                                                                                              >  1:275622/1‑139 (MQ=255)        acgacgCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAAt                                                                                                       >  1:737119/1‑139 (MQ=255)            cgCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGc                                                                                                   >  1:385090/1‑139 (MQ=255)             gCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCa                                                                                                  <  2:708391/139‑1 (MQ=255)                                caccacGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACa                                                                               <  2:737119/139‑1 (MQ=255)                                   cacGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACaaaa                                                                            >  1:680765/1‑139 (MQ=255)                                                            tCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt                                                            <  2:99100/130‑1 (MQ=255)                                                            tCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt                                                            >  1:99100/1‑130 (MQ=255)                                                              cAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTc                                                 >  1:777042/1‑139 (MQ=255)                                                                 cacacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGGAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGc                                              <  1:353495/139‑1 (MQ=255)                                                                 cacacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGc                                              >  2:455489/1‑139 (MQ=255)                                                                      aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtc                                         >  1:429025/1‑139 (MQ=255)                                                                           tGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt                                                            <  1:684777/115‑1 (MQ=255)                                                                           tGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt                                                            >  2:684777/1‑115 (MQ=255)                                                                                  tatGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGc                             >  1:780323/1‑139 (MQ=255)                                                                                  tatGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGc                             >  1:787572/1‑139 (MQ=255)                                                                                      cAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGc                                                              >  2:591066/1‑102 (MQ=255)                                                                                      cAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGc                                                              <  1:591066/102‑1 (MQ=255)                                                                                       aGTTCAACAAAGGTTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGcc                        >  1:55818/1‑139 (MQ=255)                                                                                       aGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGcc                        >  1:294009/1‑139 (MQ=255)                                                                                       aGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGcc                        >  1:467376/1‑139 (MQ=255)                                                                                       aGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGcc                        >  2:637628/1‑139 (MQ=255)                                                                                          tCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGCTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGcc                        >  1:287909/1‑139 (MQ=255)                                                                                          tCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCaa                     >  2:762695/1‑139 (MQ=255)                                                                                          tCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCaa                     >  1:111525/1‑139 (MQ=255)                                                                                                   gCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATAtttt            >  2:284340/1‑139 (MQ=255)                                                                                                   gCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATAtttt            >  1:372176/1‑139 (MQ=255)                                                                                                     tttAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTa          <  2:467376/139‑1 (MQ=255)                                                                                                            tCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGcccc   <  2:429025/139‑1 (MQ=255)                                                                                                             ccTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCa  >  2:702507/1‑139 (MQ=255)                                                                                                             acTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCa  >  2:32003/2‑139 (MQ=255)                                                                                                                  |                                                                                                                                         CCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCA  >  NC_000913/544641‑544887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑