Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	571,444	G→A	*184* (TAG→TAA)	ybcL →	inactive polymorphonuclear leukocyte migration suppressor, DLP12 prophage, UPF0098 family secreted protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	571,444	0	G	A	100.0%	263.6 / NA	80	*184* (TAG→TAA)	ybcL	inactive polymorphonuclear leukocyte migration suppressor, DLP12 prophage, UPF0098 family secreted protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/40);  total (40/40)

GTTTAAAGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAGGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGTTAT  >  NC_000913/571312‑571578                                                                                                                                     |                                                                                                                                       gTTTAAAGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTgag                                                                                                                                  >  1:265131/1‑139 (MQ=255)    tAAAGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGt                                                                                                                               <  1:65823/139‑1 (MQ=255)            gggCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGc                                                                                                                       >  2:334936/1‑139 (MQ=255)            gggCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGc                                                                                                                       >  2:53559/1‑139 (MQ=255)            gggCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGc                                                                                                                       >  1:234768/1‑139 (MQ=255)            gggCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGc                                                                                                                       >  2:455962/1‑139 (MQ=255)            gggCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGc                                                                                                                       >  1:568286/1‑139 (MQ=255)            gggCTCTAAAAACTGAAAAGATTCCTGTAGATTATAACTCCAGCGGAACGTAAGATGGTTATATGCTTAATGCTAATAAAATCGCAACAGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGAATGCTgga                                                                                                                       >  1:537429/1‑138 (MQ=255)             ggCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCa                                                                                                                      <  1:51830/139‑1 (MQ=255)             ggCTCTAAAAACTGAAAAGATCCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCa                                                                                                                      <  2:600618/139‑1 (MQ=255)              gCTCTATAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCaa                                                                                                                     <  2:49634/139‑1 (MQ=255)                  tAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAgg                                                                                                                 >  1:206964/1‑139 (MQ=255)                   aaaaaCTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGt                                                                                                                >  2:386947/1‑139 (MQ=255)                   aaaaaCTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGt                                                                                                                <  1:455962/139‑1 (MQ=255)                   aaaaaCTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGt                                                                                                                >  2:711138/1‑139 (MQ=255)                       aCTGAGAAGATTCTTGTAGATTCCAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGTTAACACCAGTTTATTAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGa                                                                                                            <  1:527935/139‑1 (MQ=255)                       aCTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGa                                                                                                            <  1:386947/139‑1 (MQ=255)                         tGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACt                                                                                                          <  2:653107/139‑1 (MQ=255)                         tGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACt                                                                                                          <  1:754022/139‑1 (MQ=255)                            aaaGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTgaga                                                                                                                                 >  1:785407/1‑113 (MQ=255)                            aaaGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTgaga                                                                                                                                 <  2:785407/113‑1 (MQ=255)                                  tCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAg                                                                                                 >  1:636468/1‑139 (MQ=255)                                    cTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGAt                                                                                               <  2:568286/139‑1 (MQ=255)                                            tCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGaaa                                                                                       >  1:66642/1‑139 (MQ=255)                                                aCTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACg                                                                                   <  1:595533/139‑1 (MQ=255)                                                 cTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACgg                                                                                  <  1:185338/139‑1 (MQ=255)                                                  tCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGa                                                                                 <  1:699153/139‑1 (MQ=255)                                                  tCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGa                                                                                 <  2:330197/139‑1 (MQ=255)                                                    cAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGAGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATg                                                                               >  2:530780/1‑139 (MQ=255)                                                     aGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACt                                                                                                          <  2:38052/111‑1 (MQ=255)                                                     aGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACt                                                                                                          >  1:38052/1‑111 (MQ=255)                                                            cGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCa                                                                                                                      <  1:682277/92‑1 (MQ=255)                                                            cGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCa                                                                                                                      >  2:682277/1‑92 (MQ=255)                                                            cGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAAca                                                                       >  1:449678/1‑139 (MQ=255)                                                                aGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAAcatcat                                                                   <  2:265131/139‑1 (MQ=255)                                                                aGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAAcatcat                                                                   <  2:449678/139‑1 (MQ=255)                                                                  ttGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATga                                                                 <  1:334936/139‑1 (MQ=255)                                                                  ttGGTTATATACTTAATGCCAATAAAATCGCAATCGCTGAGATAACACCAGTTTACAAGATGAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACCAACGTAAATATCGAACCAATACGGATGCATAAACATAATga                                                                 <  1:439032/139‑1 (MQ=255)                                                                        atatGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCa                                                           <  2:66642/139‑1 (MQ=255)                                                                         tatGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCAt                                                          <  2:718258/139‑1 (MQ=255)                                                                         tatGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATACATAAACATCATGAGATTCAt                                                          >  2:411747/1‑139 (MQ=255)                                                                           tGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTCAGGTGAGAGTATGCTGGCAAGAGGTAAGACTCACTTAAAGATCGAAGAAATACGGATGCATAAACATCATgat                                                                <  2:491587/131‑2 (MQ=255)                                                                           tGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATgag                                                                >  1:491587/1‑131 (MQ=255)                                                                           tGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAg                                                        >  1:149517/1‑139 (MQ=255)                                                                                aaTGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCAt                                                          <  1:80378/132‑1 (MQ=255)                                                                                aaTGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCAt                                                          >  2:80378/1‑132 (MQ=255)                                                                                aaTGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTa                                                   >  2:350423/1‑139 (MQ=255)                                                                                     taataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATga                                                                 >  2:125442/1‑120 (MQ=255)                                                                                     taataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATga                                                                 <  1:125442/120‑1 (MQ=255)                                                                                       ataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGa                                                                                                            >  2:167314/1‑75 (MQ=255)                                                                                       ataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGa                                                                                                            <  1:167314/75‑1 (MQ=255)                                                                                       ataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGa                                                                                 >  2:553954/1‑102 (MQ=255)                                                                                       ataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGa                                                                                 <  1:553954/102‑1 (MQ=255)                                                                                       ataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAAcatcat                                                                   <  2:9557/116‑1 (MQ=255)                                                                                       ataaAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAAcatcat                                                                   >  1:9557/1‑116 (MQ=255)                                                                                              cGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGt                                                     >  2:463022/1‑123 (MQ=255)                                                                                              cGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGt                                                     <  1:463022/123‑1 (MQ=255)                                                                                                  aCCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATga                                                                 >  2:592494/1‑107 (MQ=255)                                                                                                  aCCGCTGAGATAACACCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATga                                                                 <  1:592494/107‑1 (MQ=255)                                                                                                      cTGAGATAACACCAGTTTATGAGATAAAGTAAGGAGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTtgtg                             <  1:530780/139‑1 (MQ=255)                                                                                                               cacCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCg                    >  2:18825/1‑139 (MQ=255)                                                                                                               cacCAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCg                    >  2:163288/1‑139 (MQ=255)                                                                                                                  cAGTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCa                 <  1:350423/139‑1 (MQ=255)                                                                                                                    gTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAAcatc                                                                     <  2:153637/85‑1 (MQ=255)                                                                                                                    gTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAAcatc                                                                     >  1:153637/1‑85 (MQ=255)                                                                                                                    gTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAgg               >  2:207206/1‑139 (MQ=255)                                                                                                                    gTTTATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTAGCCGTCAgg               >  1:486059/1‑139 (MQ=255)                                                                                                                     tttATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATa                                                                                     >  1:308140/1‑68 (MQ=255)                                                                                                                     tttATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATa                                                                                     <  2:308140/68‑1 (MQ=255)                                                                                                                     tttATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACgg                                                                                  <  1:481843/71‑1 (MQ=255)                                                                                                                     tttATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACgg                                                                                  >  2:481843/1‑71 (MQ=255)                                                                                                                      ttATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGga             <  2:751472/139‑1 (MQ=255)                                                                                                                       tATGAGATAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGgaa            >  1:515076/1‑139 (MQ=255)                                                                                                                          gagaTAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGgaaaga         >  2:52635/1‑139 (MQ=255)                                                                                                                            gaTAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTtg                               >  1:131089/1‑115 (MQ=255)                                                                                                                            gaTAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTtg                               <  2:131089/115‑1 (MQ=255)                                                                                                                             aTAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCa                                                                             >  1:82119/1‑68 (MQ=255)                                                                                                                             aTAAAGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCa                                                                             <  2:82119/68‑1 (MQ=255)                                                                                                                               aaaGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGtt    <  2:515076/139‑1 (MQ=255)                                                                                                                                 aGTAAGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGTTAt  >  2:260600/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                       GTTTAAAGTATGGGCTCTAAAAACTGAAAAGATTCCTGTAGATTCTAACTCCAGCGGAGCGTTAGTTGGTTATATGCTTAATGCTAATAAAATCGCAACCGCTGAGATAACACCAGTTTATGAGATAAAGTAGGGTGAGAGTATGCTGGCAAGAGGTAAGACTAACTTAAAGATCGAAGAAATACGGATGCATAAACATCATGAGATTCATAGGGTTAAGCCTCTTATGCCAGCTTTGTGTCGTATCCGTCAGGGAAAGAAAGTTAT  >  NC_000913/571312‑571578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑