Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 572,454 C→T C29C (TGC→TGT ylcH → uncharacterized protein, DLP12 prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913572,4540CT100.0% 98.3 / NA 31C29C (TGC→TGTylcHuncharacterized protein, DLP12 prophage
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/14);  total (17/14)

CGGAGCCTGAACAACTCCGGTGACTTCTGCGCTAAACGGGGACGTTTATGCGCACATACAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGCGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCTTATCTGGAGGAGGGCAAATGCTTTCGCATGGTGCT  >  NC_000913/572321‑572572
                                                                                                                                     |                                                                                                                      
cGGAGCCTGAACAACTCCGGTGACTTCTGCGCTAAACGGGGACGTTTATGCGCACATACAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAgg                                                                                                                   >  2:689002/1‑139 (MQ=255)
            aacTCCGGTGACTTCTGCGCTAAACGGGGACGTTTATGCGCACATACAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAAc                                                                                                       <  1:675439/139‑1 (MQ=255)
                ccGGTGACTTCTGCGCTAAACGGGGACGTTTATGCGCACATACAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACctct                                                                                                   >  2:629065/1‑139 (MQ=255)
                        ttCTGCGCTAAACGGGGACGTTTATGCGCACATACAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGAc                                                                                           <  2:608214/139‑1 (MQ=255)
                          cTGCGCTAAACGGGGACGTTTATGCGCACATACAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACgg                                                                                         <  2:515813/139‑1 (MQ=255)
                          cTGCGCTAAACGGGGACGTTTATGCGCACATACAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACgg                                                                                         <  2:319240/139‑1 (MQ=255)
                            gcgcTAAACGGGGACGTTTATGCGCACATACAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCa                                                                                       <  2:460172/139‑1 (MQ=255)
                                                          cAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTa                                                         >  2:150834/1‑139 (MQ=255)
                                                             tCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTAtcg                                                      >  2:136277/1‑139 (MQ=255)
                                                             tCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTAtcg                                                      >  2:713390/1‑139 (MQ=255)
                                                                 aaCTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACgg                                                                                         <  1:59331/100‑1 (MQ=255)
                                                                 aaCTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACgg                                                                                         >  2:59331/1‑100 (MQ=255)
                                                                  aCTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTAtcggtcgg                                                 <  1:150834/139‑1 (MQ=255)
                                                                       cttctCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGAt                                            >  2:558118/1‑139 (MQ=255)
                                                                             ccTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGcc                                      <  1:765307/139‑1 (MQ=255)
                                                                             ccTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGcc                                      <  2:555419/139‑1 (MQ=255)
                                                                              cTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCt                                     >  2:631810/1‑139 (MQ=255)
                                                                                tCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTATGGAGGGGAAGCGTGaa                                                                                                        >  1:220840/1‑70 (MQ=255)
                                                                                tCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTATGGAGGGGAAGCGTGaa                                                                                                        <  2:220840/70‑1 (MQ=255)
                                                                                      aTGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCa                                                                                       >  2:384910/1‑81 (MQ=255)
                                                                                      aTGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCa                                                                                       <  1:384910/81‑1 (MQ=255)
                                                                                         cAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCATAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCTTATCTggagga                          >  2:497238/1‑139 (MQ=255)
                                                                                                gCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGTCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGcc                                      >  2:497895/1‑120 (MQ=255)
                                                                                                gCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGcc                                      <  1:497895/120‑1 (MQ=255)
                                                                                                gCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCTTATCTGGAGGAGGGCaaa                   >  1:79240/1‑139 (MQ=255)
                                                                                                   ccTGCAATTCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCTTATCTGGAGGAGGGCAAATGc                >  2:567133/1‑139 (MQ=255)
                                                                                                          ttCTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCTTATCTGGAGGAGGGCAAATGCTTTCGCa         >  2:349497/1‑139 (MQ=255)
                                                                                                            cTTTGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCCCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCTTATCTGGAGGAGGGCAAATGCTTTCGCATg       <  1:553760/139‑1 (MQ=255)
                                                                                                               tGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCgg                                                                       <  2:311357/72‑1 (MQ=255)
                                                                                                               tGCATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCgg                                                                       >  1:311357/1‑72 (MQ=255)
                                                                                                                 cATCTAGCGTTTGACCTCTGTGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCTTATCTGGAGGAGGCCAAATGCTTTCGCATGGTGCt  >  2:470120/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                      
CGGAGCCTGAACAACTCCGGTGACTTCTGCGCTAAACGGGGACGTTTATGCGCACATACAATCCAAACTCTCTTCTCCCTTCACAGATGCAGAAATGCACCTGCAATTCTTTGCATCTAGCGTTTGACCTCTGCGGAGGGGAAGCGTGAACCTCTCACAAGACGGCATCAAATTACATCGCGGCAACTTCACCGCTATCGGTCGGCAGATCCAGCCTTATCTGGAGGAGGGCAAATGCTTTCGCATGGTGCT  >  NC_000913/572321‑572572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: