Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 66,447 | T→C | E35G (GAG→GGG) | araD ← | L‑ribulose‑5‑phosphate 4‑epimerase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 66,447 | 0 | T | C | 100.0% | 90.5 / NA | 26 | E35G (GAG→GGG) | araD | L‑ribulose‑5‑phosphate 4‑epimerase |
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/12); total (14/12) |
TCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTT > NC_000913/66324‑66582 | tCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGgcgc > 1:388699/1‑139 (MQ=255) aggaggGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATCTCGTCAGCGGTCATGACGCTGTAATCGACGCCGGTAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGAc < 2:258310/139‑1 (MQ=255) gaggGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCACGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGt < 1:465616/139‑1 (MQ=255) gCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGcc < 1:269287/139‑1 (MQ=255) gCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGcc < 2:250565/139‑1 (MQ=255) tCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACg > 2:727357/1‑120 (MQ=255) tCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACg < 1:727357/120‑1 (MQ=255) ttCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTtg > 2:215901/1‑139 (MQ=255) tGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTgag < 2:464225/139‑1 (MQ=255) tGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTgag < 2:388699/139‑1 (MQ=255) tGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTg < 2:101440/110‑1 (MQ=255) tGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTg > 1:101440/1‑110 (MQ=255) gTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGAtcttct < 1:357282/139‑1 (MQ=255) cGCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGCCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGtt > 1:565544/1‑139 (MQ=255) gCCGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTg > 1:594254/1‑139 (MQ=255) cGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCg > 1:720235/1‑66 (MQ=255) cGGAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCg < 2:720235/66‑1 (MQ=255) gAAGGTTTGATCACAAAGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTc > 1:539091/1‑139 (MQ=255) aaaGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATg > 1:802389/1‑139 (MQ=255) aaaGACGCCGCGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATg > 2:103312/1‑139 (MQ=255) aaGACGCCGAGCCCGCGATCACCGGTGCTGACGTTGCCCCATGTGAGCGTGTCCAGGTTGCGGTTTGTCAGCGCCAGGTTGGCTTTTAATAGCTGGCGTTTGATATATACTAACATGTTGACTCCTTCATGCCGGAttc > 1:512039/1‑137 (MQ=255) cgccgcGCCCGCGATCAACGGCGCTGACGTTGCCCCAtg < 2:733998/39‑1 (MQ=255) cgccgcGCCCGCGATCAACGGCGCTGACGTTGCCCCAtg > 1:733998/1‑39 (MQ=255) ccgcGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGTGCCAGGTTGGCTTCTAATACCTGGCGTTTgag < 1:476069/98‑1 (MQ=255) ccgcGCCCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTgag > 2:476069/1‑98 (MQ=255) cgcCCGCGATCAACGACGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGAGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCtt > 1:534448/1‑139 (MQ=255) | TCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTT > NC_000913/66324‑66582 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |