Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	677,889	G→A	A159T (GCC→ACC)	djlB →	putative HscC co‑chaperone, uncharacterized J domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	677,889	0	G	A	100.0%	122.2 / NA	38	A159T (GCC→ACC)	djlB	putative HscC co‑chaperone, uncharacterized J domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/17);  total (21/17)

GTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTG  >  NC_000913/677753‑678014                                                                                                                                         |                                                                                                                              gTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATAcc                                                                                                                             >  1:203577/1‑139 (MQ=255) gTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATAcc                                                                                                                             >  2:275239/1‑139 (MQ=255)         gCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGCGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGaa                                                                                                                     >  2:630544/1‑139 (MQ=255)                cGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAAt                                                                                                              >  1:328937/1‑139 (MQ=255)                         cacTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAAc                                                                                                     >  2:211102/1‑139 (MQ=255)                                           cGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCAt                                                                                   <  1:211102/139‑1 (MQ=255)                                             aGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCt                                                                                 >  1:31004/1‑139 (MQ=255)                                             aGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCt                                                                                 <  2:203577/139‑1 (MQ=255)                                                 cGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGccc                                                                             >  1:348300/1‑139 (MQ=255)                                                 cGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGccc                                                                             >  1:674587/1‑139 (MQ=255)                                                                aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGttttt                                                                                                        >  2:792709/1‑97 (MQ=255)                                                                aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGttttt                                                                                                        <  1:792709/97‑1 (MQ=255)                                                                aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAAt                                                              >  1:31707/1‑139 (MQ=255)                                                                aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAAt                                                              >  2:63659/1‑139 (MQ=255)                                                                aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAAt                                                              >  2:59167/1‑139 (MQ=255)                                                                aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAAt                                                              >  2:590777/1‑139 (MQ=255)                                                                aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAAt                                                              >  1:273085/1‑139 (MQ=255)                                                                  aaaGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGa                                                            <  2:31004/139‑1 (MQ=255)                                                                       ttGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCa                                                       <  2:297748/139‑1 (MQ=255)                                                                                   cAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTAcc                                           <  1:637877/139‑1 (MQ=255)                                                                                     ggaggaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCaa                                         >  2:325487/1‑139 (MQ=255)                                                                                         gaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGt                                     <  2:674587/139‑1 (MQ=255)                                                                                         gaAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGt                                     >  2:641020/1‑139 (MQ=255)                                                                                          aaaaTACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGtt                                    >  1:388799/1‑139 (MQ=255)                                                                                                   gggAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGCGAATGTTTTTAACTTCCAGACTATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCATATGTTCGCTCGGAt                           >  2:490357/1‑139 (MQ=255)                                                                                                    ggAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGAtt                          >  1:448159/1‑139 (MQ=255)                                                                                                        aTAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGt                      <  1:347107/139‑1 (MQ=255)                                                                                                         tAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTc                     <  2:388799/139‑1 (MQ=255)                                                                                                           gACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCAt                   <  2:392811/139‑1 (MQ=255)                                                                                                                    gaaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGa                                                                                              >  1:102963/1‑55 (MQ=255)                                                                                                                    gaaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGa                                                                                              <  2:102963/55‑1 (MQ=255)                                                                                                                     aaCTGAAATCCTGTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGAAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCcaca         <  1:550888/139‑1 (MQ=255)                                                                                                                      aCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACaa        >  2:255302/1‑139 (MQ=255)                                                                                                                          gAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGc    <  2:448159/139‑1 (MQ=255)                                                                                                                          gAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGc    <  1:63659/139‑1 (MQ=255)                                                                                                                            aTCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTg  <  1:641020/139‑1 (MQ=255)                                                                                                                            aTCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTg  <  2:323292/139‑1 (MQ=255)                                                                                                                            aTCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTg  >  2:779926/1‑139 (MQ=255)                                                                                                                            aTCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTg  <  2:273085/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                              GTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTG  >  NC_000913/677753‑678014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑