Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	679,874	G→A	G123R (GGG→AGG)	ybeU →	DUF1266 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	679,874	0	G	A	100.0%	142.3 / NA	44	G123R (GGG→AGG)	ybeU	DUF1266 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/25);  total (19/25)

TCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTA  >  NC_000913/679738‑680001                                                                                                                                         |                                                                                                                                tCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATgagg                                                                                                                               <  1:193128/139‑1 (MQ=255)    ctctTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGttt                                                                                                                            <  1:676186/139‑1 (MQ=255)        tCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGt                                                                                                                        >  1:378562/1‑139 (MQ=255)               aaGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGt                                                                                                                 >  1:342482/1‑139 (MQ=255)                 gagTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCg                                                                                                               <  2:402057/139‑1 (MQ=255)                   gTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGgt                                                                                                             <  1:769627/139‑1 (MQ=255)                    tGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGgtg                                                                                                            <  1:200013/139‑1 (MQ=255)                    tGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGgtg                                                                                                            >  2:313918/1‑139 (MQ=255)                    tGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGgt                                                                                                             >  1:525755/1‑138 (MQ=255)                    tGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGgt                                                                                                             <  2:525755/138‑1 (MQ=255)                    tGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTATCTGTCACGGGTCAgtg                                                                                                            <  1:117648/139‑1 (MQ=255)                            aTTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTcaac                                                                                                    <  1:630346/139‑1 (MQ=255)                                    cTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCt                                                                                            >  2:783238/1‑139 (MQ=255)                                     tggtggCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTg                                                                                           >  2:589987/1‑139 (MQ=255)                                      ggtggCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGATGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGa                                                                                          >  1:681394/1‑139 (MQ=255)                                          gCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGga                                                                                      >  1:65356/1‑139 (MQ=255)                                                                     aaTTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcg                                                           >  2:189559/1‑139 (MQ=255)                                                                      aTTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcgc                                                          >  2:25940/1‑139 (MQ=255)                                                                               ccAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTa                                                 <  1:407762/139‑1 (MQ=255)                                                                                 aaaCCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACg                                               <  2:60264/139‑1 (MQ=255)                                                                                   aCCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAg                                             <  2:785146/139‑1 (MQ=255)                                                                                   aCCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAg                                             <  2:127119/139‑1 (MQ=255)                                                                                     cGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCt                                           <  2:95021/139‑1 (MQ=255)                                                                                     cGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCt                                           <  2:704858/139‑1 (MQ=255)                                                                                      gCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTc                                          <  2:342482/139‑1 (MQ=255)                                                                                           gtgCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCAt                                     <  1:785229/139‑1 (MQ=255)                                                                                                  ggggAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATc                              >  2:526303/1‑139 (MQ=255)                                                                                                    ggAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcgc                                                          <  1:549256/109‑1 (MQ=255)                                                                                                    ggAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcgc                                                          >  2:549256/1‑109 (MQ=255)                                                                                                      aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAg                          >  2:241550/1‑139 (MQ=255)                                                                                                      aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAg                          <  1:253564/139‑1 (MQ=255)                                                                                                      aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAg                          <  2:468081/139‑1 (MQ=255)                                                                                                           ggAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCa                                         >  1:709023/1‑119 (MQ=255)                                                                                                           ggAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCa                                         <  2:709023/119‑1 (MQ=255)                                                                                                             aaTCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATg                   <  1:30745/139‑1 (MQ=255)                                                                                                                  aaGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTa              >  1:119806/1‑139 (MQ=255)                                                                                                                   aGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTAt             >  2:627947/1‑139 (MQ=255)                                                                                                                       tGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTAttttt         <  1:589987/139‑1 (MQ=255)                                                                                                                         ggATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGc                                                                                                         >  2:166187/1‑41 (MQ=255)                                                                                                                         ggATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGc                                                                                                         <  1:166187/41‑1 (MQ=255)                                                                                                                         ggATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTcaac                                                                                                    >  1:573062/1‑46 (MQ=255)                                                                                                                         ggATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTcaac                                                                                                    <  2:573062/46‑1 (MQ=255)                                                                                                                         ggATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTcc       <  2:681394/139‑1 (MQ=255)                                                                                                                              aTGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTa  <  2:341386/139‑1 (MQ=255)                                                                                                                              aTGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTa  >  1:130026/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                TCGCTCTTCCCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTATTTTTCCGCCTA  >  NC_000913/679738‑680001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑