Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	789,464	G→A	I172I (ATC→ATT)	galK ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	789,464	0	G	A	100.0%	54.3 / NA	18	I172I (ATC→ATT)	galK	galactokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/8);  total (10/8)

GATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACT  >  NC_000913/789350‑789598                                                                                                                   |                                                                                                                                       gatgatgaCGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAAc                                                                                                                <  2:188445/139‑1 (MQ=255)         cgacAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTc                                                                                                        >  1:666468/1‑139 (MQ=255)                 acacCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCt                                                                                                >  1:639661/1‑139 (MQ=255)                    ccTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGAc                                                                                             >  2:471665/1‑139 (MQ=255)                               ggAAACTGCTTTGGTCCCCAGTGAGCGGCAATTGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGcgcg                                                                                  >  1:56084/1‑139 (MQ=255)                               ggAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCATTAAGcgcg                                                                                  >  2:512207/1‑139 (MQ=255)                                  aaCTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGAtt                                                                               <  1:471665/139‑1 (MQ=255)                                          tGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCt                                                                                                    <  1:288559/110‑1 (MQ=255)                                          tGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCt                                                                                                    >  2:288559/1‑110 (MQ=255)                                           ggTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCg                                                                      >  1:442028/1‑139 (MQ=255)                                             tCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTc                                                                    >  2:459495/1‑139 (MQ=255)                                                                     gCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGctgctg                                            <  1:512207/139‑1 (MQ=255)                                                                                    tcttGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGAc                             >  1:600092/1‑139 (MQ=255)                                                                                        gCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACcgcg                         <  2:102687/139‑1 (MQ=255)                                                                                                     aTTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAg            <  2:442028/139‑1 (MQ=255)                                                                                                        aGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCgg         <  2:600092/139‑1 (MQ=255)                                                                                                               ccATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACt  >  2:266257/1‑139 (MQ=255)                                                                                                               ccATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACt  <  2:56084/139‑1 (MQ=255)                                                                                                                   |                                                                                                                                       GATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACT  >  NC_000913/789350‑789598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑