Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,068,254 G→A *58* (TAG→TAA ymdF → KGG family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,068,2540GA100.0% 81.1 / NA 26*58* (TAG→TAAymdFKGG family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/15);  total (11/15)

GACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGA  >  NC_000913/1068120‑1068389
                                                                                                                                      |                                                                                                                                       
gaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCgg                                                                                                                                     <  1:193904/139‑1 (MQ=255)
 aCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCggg                                                                                                                                    <  2:791594/139‑1 (MQ=255)
 aCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCggg                                                                                                                                    <  2:133860/139‑1 (MQ=255)
             cATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGAc                                                                                                                        >  2:35730/1‑139 (MQ=255)
                                    ggACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGt                                                                                               >  1:434774/1‑139 (MQ=255)
                                     gACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTc                                                                                              >  2:274141/1‑139 (MQ=255)
                                               cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCAt                                                                                    >  1:537544/1‑139 (MQ=255)
                                                             aGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAcc                                                                      <  2:434774/139‑1 (MQ=255)
                                                               aaTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  1:49954/139‑1 (MQ=255)
                                                                  gACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtg                                                                 >  2:751864/1‑139 (MQ=255)
                                                                                 tCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGAc                                                  <  2:436465/139‑1 (MQ=255)
                                                                                 tCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGAc                                                  <  1:35730/139‑1 (MQ=255)
                                                                                      aGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATg                                             <  2:537544/139‑1 (MQ=255)
                                                                                           gTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATTATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    >  1:546700/1‑111 (MQ=255)
                                                                                           gTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  2:546700/111‑1 (MQ=255)
                                                                                            tAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCt                                       >  2:647772/1‑139 (MQ=255)
                                                                                                    gtggtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGAt                               >  2:714436/1‑139 (MQ=255)
                                                                                                     tggtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCAttt                                                                                                              >  2:264657/1‑60 (MQ=255)
                                                                                                     tggtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCAttt                                                                                                              <  1:264657/60‑1 (MQ=255)
                                                                                                     tggtAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATg                              >  2:259430/1‑139 (MQ=255)
                                                                                                          agagCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGa                         <  1:78441/139‑1 (MQ=255)
                                                                                                            agCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATg                       <  1:647772/139‑1 (MQ=255)
                                                                                                                     ggCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTTATGCGCGATGCTTATCAgg              <  1:104514/139‑1 (MQ=255)
                                                                                                                     ggCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCAATTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAgg              <  2:551022/139‑1 (MQ=255)
                                                                                                                      gCAAAAGCGACAACTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGc             >  1:11842/1‑139 (MQ=255)
                                                                                                                                 aaCTAACCGGGCTAATCAATGACGAATCATTTTTGCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaaga  <  1:259430/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                       
GACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGA  >  NC_000913/1068120‑1068389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: